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The map label for this gene is pdhC [H]

Identifier: 78778785

GI number: 78778785

Start: 379566

End: 380933

Strand: Direct

Name: pdhC [H]

Synonym: PMT9312_0400

Alternate gene names: 78778785

Gene position: 379566-380933 (Clockwise)

Preceding gene: 78778784

Following gene: 78778786

Centisome position: 22.21

GC content: 36.04

Gene sequence:

>1368_bases
ATGTCTCACGAAATATTCATGCCTGCCTTGAGTTCTACCATGACAGAGGGCAAGATTGTGGAATGGTTGAAAAATCCTGG
AGATAAGGTTGAAAGGGGTGAATCTGTCCTAGTTGTTGAATCTGATAAGGCAGATATGGATGTTGAATCTTTTCAAGATG
GATATCTTGCAGCAGTTTTAATGCCTGCTGGTAGCACTGCACCAGTAGGGGAAACTATAGGTCTTATTGTAGAAAATGAG
GATGAGATAGCTTCTGTTAAAGAACAAAATAAAGGAAATCAACCCGAAGTTTCTAGTTCGGATAAACTTGAATTGGTAAG
CAATAAAACCGAAGAAAAACCGGAAGTACATAATGAAAATGTTAAAAAAGAAGAAAAAGAAGTCGTCTTAAAGAGTGAAA
AGTCAGCCCCATCTTTTAATAGTGATCAAATTAATGCTGCTACAAGTAATGTTTCTTCAAGGGTAATTGCATCTCCAAGA
GCTAAAAAACTTGCCTCTCAAATGGGTGTTGACTTAGCAAAAGTTCACGGATCTGGACCTCACGGAAGAATTCAAGCAGA
TGATATTTTAAAAGCTAATGGTCAACCTGTATCTATTCCATGGATAGGAGAGGGTAGTTCTCCTGCAAGTATTCCTGGTG
CAAATTTACAAGTTGAAAGTAAACCAGAAACATCAGGAAATAGTTTTGGTAATCCTGGAGAAACAGTGCAATTTAATACT
CTTCAAAAAGCGGTAAATAAAAATATGGAGTCTAGTTTGGATATTCCATGTTTTAGGGTGGGATACTCTATAAACACAGA
TAAATTAGATAATTTCTACAAAAAGGTAAAACAAAATGGAGTCACTATGACTGCTTTACTAGTAAAGGCAGTTGCAAAGA
CAATTAAGAAACATCCTCAAGTTAACTCAAGTTTTTCAGAAAATGGAATTTCTTATCCAGAAAATATTAATATTGCTGTT
GCCGTCGCAATGGAAGATGGCGGATTAATAACTCCAGTTTTAAAAGAACCTTGCAATACTGATTTATTTGAATTATCTAG
GGAATGGAAAGATTTGGTAAAAAGATCAAGATCAAAACAATTAGAACCTGATGAGTACTCAACAGGAACCTTTACTTTAT
CTAACCTTGGTATGTTTGGAGTTGATAGATTTGACGCAATTCTACCCCCAGGTACTGGTGCTATTTTAGCGATAGCATCA
TCGAAACCAACTGTTGTTGCTAATAGTGATGGCTCAATATCTGTTAAAAAAATTATGCAAGTAAATCTAACCGCTGATCA
CAGAGTGATCTATGGAGCTGATGGTGCTTCATTCTTAAAAGATTTGGCTTCCCTGATAGAAAATGAGCCAGAGACACTTG
TATCTTAA

Upstream 100 bases:

>100_bases
TTTCGTATAATTAAATCAAGAGTTTAAAGGGTTTTAAATCATAAAAACTTTAGAAAGTTTGATAGAAATCTTTTAAAACT
TATGCAATAACAATTTTCTT

Downstream 100 bases:

>100_bases
ATTTAATTGATTTCTCAAATTAATAATGAAGAAAGAGATTATAAGCTTGAAGCTTATGATTACTTTCTTGATCCTTCATT
AATTGCTAGTAAACCTTCTG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENE
DEIASVKEQNKGNQPEVSSSDKLELVSNKTEEKPEVHNENVKKEEKEVVLKSEKSAPSFNSDQINAATSNVSSRVIASPR
AKKLASQMGVDLAKVHGSGPHGRIQADDILKANGQPVSIPWIGEGSSPASIPGANLQVESKPETSGNSFGNPGETVQFNT
LQKAVNKNMESSLDIPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTIKKHPQVNSSFSENGISYPENINIAV
AVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIAS
SKPTVVANSDGSISVKKIMQVNLTADHRVIYGADGASFLKDLASLIENEPETLVS

Sequences:

>Translated_455_residues
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENE
DEIASVKEQNKGNQPEVSSSDKLELVSNKTEEKPEVHNENVKKEEKEVVLKSEKSAPSFNSDQINAATSNVSSRVIASPR
AKKLASQMGVDLAKVHGSGPHGRIQADDILKANGQPVSIPWIGEGSSPASIPGANLQVESKPETSGNSFGNPGETVQFNT
LQKAVNKNMESSLDIPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTIKKHPQVNSSFSENGISYPENINIAV
AVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIAS
SKPTVVANSDGSISVKKIMQVNLTADHRVIYGADGASFLKDLASLIENEPETLVS
>Mature_454_residues
SHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENED
EIASVKEQNKGNQPEVSSSDKLELVSNKTEEKPEVHNENVKKEEKEVVLKSEKSAPSFNSDQINAATSNVSSRVIASPRA
KKLASQMGVDLAKVHGSGPHGRIQADDILKANGQPVSIPWIGEGSSPASIPGANLQVESKPETSGNSFGNPGETVQFNTL
QKAVNKNMESSLDIPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTIKKHPQVNSSFSENGISYPENINIAVA
VAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASS
KPTVVANSDGSISVKKIMQVNLTADHRVIYGADGASFLKDLASLIENEPETLVS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=458, Percent_Identity=29.4759825327511, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI203098753, Length=475, Percent_Identity=28, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI203098816, Length=475, Percent_Identity=28, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI110671329, Length=453, Percent_Identity=26.9315673289183, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI260898739, Length=158, Percent_Identity=34.1772151898734, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI19923748, Length=172, Percent_Identity=30.8139534883721, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786305, Length=298, Percent_Identity=30.5369127516779, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1786946, Length=461, Percent_Identity=22.9934924078091, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=468, Percent_Identity=30.5555555555556, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=452, Percent_Identity=23.6725663716814, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=29.0849673202614, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25146366, Length=180, Percent_Identity=33.8888888888889, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324258, Length=465, Percent_Identity=32.6881720430108, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6320352, Length=193, Percent_Identity=29.5336787564767, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321632, Length=88, Percent_Identity=40.9090909090909, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582497, Length=311, Percent_Identity=30.8681672025724, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI20129315, Length=311, Percent_Identity=30.8681672025724, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI18859875, Length=323, Percent_Identity=30.3405572755418, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24645909, Length=198, Percent_Identity=29.2929292929293, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48920; Mature: 48788

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVL
CCCCEECHHHHHHCCCCHHHHHHCCCCCHHHCCCEEEEEECCCCCCCHHHHCCCEEEEEE
MPAGSTAPVGETIGLIVENEDEIASVKEQNKGNQPEVSSSDKLELVSNKTEEKPEVHNEN
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
VKKEEKEVVLKSEKSAPSFNSDQINAATSNVSSRVIASPRAKKLASQMGVDLAKVHGSGP
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHCCCHHHHHHHHHCCEEEECCCCCC
HGRIQADDILKANGQPVSIPWIGEGSSPASIPGANLQVESKPETSGNSFGNPGETVQFNT
CCCEEHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEHHH
LQKAVNKNMESSLDIPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTIKKHPQ
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VNSSFSENGISYPENINIAVAVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQ
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
LEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASSKPTVVANSDGSISVKKIMQ
CCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEEEEE
VNLTADHRVIYGADGASFLKDLASLIENEPETLVS
EECCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVL
CCCEECHHHHHHCCCCHHHHHHCCCCCHHHCCCEEEEEECCCCCCCHHHHCCCEEEEEE
MPAGSTAPVGETIGLIVENEDEIASVKEQNKGNQPEVSSSDKLELVSNKTEEKPEVHNEN
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
VKKEEKEVVLKSEKSAPSFNSDQINAATSNVSSRVIASPRAKKLASQMGVDLAKVHGSGP
CHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHCCCHHHHHHHHHCCEEEECCCCCC
HGRIQADDILKANGQPVSIPWIGEGSSPASIPGANLQVESKPETSGNSFGNPGETVQFNT
CCCEEHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEEHHH
LQKAVNKNMESSLDIPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTIKKHPQ
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VNSSFSENGISYPENINIAVAVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRSKQ
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
LEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASSKPTVVANSDGSISVKKIMQ
CCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEEEEE
VNLTADHRVIYGADGASFLKDLASLIENEPETLVS
EECCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA