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The map label for this gene is queA

Identifier: 78778786

GI number: 78778786

Start: 380940

End: 382076

Strand: Direct

Name: queA

Synonym: PMT9312_0401

Alternate gene names: 78778786

Gene position: 380940-382076 (Clockwise)

Preceding gene: 78778785

Following gene: 78778791

Centisome position: 22.29

GC content: 30.61

Gene sequence:

>1137_bases
TTGATTTCTCAAATTAATAATGAAGAAAGAGATTATAAGCTTGAAGCTTATGATTACTTTCTTGATCCTTCATTAATTGC
TAGTAAACCTTCTGCAATAAGGCATGAATCAAGATTGATGATAGTTAGAAATAGTGTTTTAGAAGAGGACTGCTTAACTA
ATAAATTTACCAAGAATCTTTTAGATGAATTTAGAGAAGGCGATCTTGTGGTTGTAAATAATACTAAAGTAATGAAGGCA
AGGTTAAAGGTTGAATTAGAAAATAGGACGTTAGTCGAATTATTAGTTTTAGAAAGATCCGATGAATGTGTTTGGTTATG
TTTAGCAAAGCCTGCAAAAAAGTTAAAAATAAATAGAAAATTAAAATTAAAATCCCCTTCTGAACAAGATATTAATTTGA
TGGTTGATGGGGTTGATGAAGAAACTGGAGGGAGATTTATTAAATTTCCGGAAAACATAACTGATCTCAATTCAATGAAT
AACCTTCTTGATAAATACGGGGAAATTCCTCTCCCTCCTTATATAAAAAATTCCGAGGAAGAATCTTTTCACGAGAACAG
TTATCAAACTGAGTATGCAATTAATCCAGGGGCAGTTGCTGCCCCAACAGCTGGTTTACACTTAAGCAAAAGTCTTATTT
CCAATCTAAAAAAAAAAGGTGTAATAATCTTGCCGATTACTTTGCACGTGGGTTATGGAACATTTAAACCAATTGATCAA
GAAGATTTAACTAACTTAAAACTTCATAAAGAATGGGTAAGTGTTAATAAGGAAGTAGTGGAGGAAATAAAAAGAATAAA
GAAAACAGATAGAAGAATAATTGCTATTGGGACAACTAGCGTAAGAGCTCTTGAAAGTTGTTATTCTCACGAAATTAATG
ACTTTATTCCCATAGCAAAGTATGTAGATTTAGTAATTAAGCCAGGTTATAAATTTAGGGTAGTTGATGGATTATTAACT
AATTTTCATCTTCCTAAAAGTTCGTTATTACTATTAGTAAGTGCAATGATTGGTAGAGAAAGATTATTAGATTTGTATAA
AAAAGCCATAAAAGAAAAATTTAGATTCTTCTCTTATGGCGATGCTATGTATATTTCACCAGATTCATTACTGGAGAAAA
ATAGATTTAAGCTTTGA

Upstream 100 bases:

>100_bases
TGATCACAGAGTGATCTATGGAGCTGATGGTGCTTCATTCTTAAAAGATTTGGCTTCCCTGATAGAAAATGAGCCAGAGA
CACTTGTATCTTAAATTTAA

Downstream 100 bases:

>100_bases
CTGGTTCTTGAATGATTCCGCTTGGAACTTCATTAAACATAATTGATGATAAATATCTCTCTGCTAAATCAGGTAGAACA
ACTACAATAATTTTTCCTGC

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MISQINNEERDYKLEAYDYFLDPSLIASKPSAIRHESRLMIVRNSVLEEDCLTNKFTKNLLDEFREGDLVVVNNTKVMKA
RLKVELENRTLVELLVLERSDECVWLCLAKPAKKLKINRKLKLKSPSEQDINLMVDGVDEETGGRFIKFPENITDLNSMN
NLLDKYGEIPLPPYIKNSEEESFHENSYQTEYAINPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
EDLTNLKLHKEWVSVNKEVVEEIKRIKKTDRRIIAIGTTSVRALESCYSHEINDFIPIAKYVDLVIKPGYKFRVVDGLLT
NFHLPKSSLLLLVSAMIGRERLLDLYKKAIKEKFRFFSYGDAMYISPDSLLEKNRFKL

Sequences:

>Translated_378_residues
MISQINNEERDYKLEAYDYFLDPSLIASKPSAIRHESRLMIVRNSVLEEDCLTNKFTKNLLDEFREGDLVVVNNTKVMKA
RLKVELENRTLVELLVLERSDECVWLCLAKPAKKLKINRKLKLKSPSEQDINLMVDGVDEETGGRFIKFPENITDLNSMN
NLLDKYGEIPLPPYIKNSEEESFHENSYQTEYAINPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
EDLTNLKLHKEWVSVNKEVVEEIKRIKKTDRRIIAIGTTSVRALESCYSHEINDFIPIAKYVDLVIKPGYKFRVVDGLLT
NFHLPKSSLLLLVSAMIGRERLLDLYKKAIKEKFRFFSYGDAMYISPDSLLEKNRFKL
>Mature_378_residues
MISQINNEERDYKLEAYDYFLDPSLIASKPSAIRHESRLMIVRNSVLEEDCLTNKFTKNLLDEFREGDLVVVNNTKVMKA
RLKVELENRTLVELLVLERSDECVWLCLAKPAKKLKINRKLKLKSPSEQDINLMVDGVDEETGGRFIKFPENITDLNSMN
NLLDKYGEIPLPPYIKNSEEESFHENSYQTEYAINPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
EDLTNLKLHKEWVSVNKEVVEEIKRIKKTDRRIIAIGTTSVRALESCYSHEINDFIPIAKYVDLVIKPGYKFRVVDGLLT
NFHLPKSSLLLLVSAMIGRERLLDLYKKAIKEKFRFFSYGDAMYISPDSLLEKNRFKL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=38.6111111111111, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PROM9 (Q31CD3)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_396898.1
- HSSP:   O32054
- ProteinModelPortal:   Q31CD3
- SMR:   Q31CD3
- STRING:   Q31CD3
- GeneID:   3765198
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0401
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   CYSHEIN
- ProtClustDB:   CLSK921848
- BioCyc:   PMAR74546:PMT9312_0401-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 43383; Mature: 43383

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISQINNEERDYKLEAYDYFLDPSLIASKPSAIRHESRLMIVRNSVLEEDCLTNKFTKNL
CCCCCCCCCCCEEEEEEHEECCCHHHCCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHH
LDEFREGDLVVVNNTKVMKARLKVELENRTLVELLVLERSDECVWLCLAKPAKKLKINRK
HHHHCCCCEEEECCCEEEEEEEEEEECCCEEEEEEEEECCCCEEEEEECCCCHHEEECCE
LKLKSPSEQDINLMVDGVDEETGGRFIKFPENITDLNSMNNLLDKYGEIPLPPYIKNSEE
EEECCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCH
ESFHENSYQTEYAINPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
EDLTNLKLHKEWVSVNKEVVEEIKRIKKTDRRIIAIGTTSVRALESCYSHEINDFIPIAK
HHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCHHHHHHHHH
YVDLVIKPGYKFRVVDGLLTNFHLPKSSLLLLVSAMIGRERLLDLYKKAIKEKFRFFSYG
HHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
DAMYISPDSLLEKNRFKL
CEEEECHHHHHHHCCCCC
>Mature Secondary Structure
MISQINNEERDYKLEAYDYFLDPSLIASKPSAIRHESRLMIVRNSVLEEDCLTNKFTKNL
CCCCCCCCCCCEEEEEEHEECCCHHHCCCCHHHHHCCEEEEEHHHHHHHHHHHHHHHHHH
LDEFREGDLVVVNNTKVMKARLKVELENRTLVELLVLERSDECVWLCLAKPAKKLKINRK
HHHHCCCCEEEECCCEEEEEEEEEEECCCEEEEEEEEECCCCEEEEEECCCCHHEEECCE
LKLKSPSEQDINLMVDGVDEETGGRFIKFPENITDLNSMNNLLDKYGEIPLPPYIKNSEE
EEECCCCCCCCEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCH
ESFHENSYQTEYAINPGAVAAPTAGLHLSKSLISNLKKKGVIILPITLHVGYGTFKPIDQ
HHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
EDLTNLKLHKEWVSVNKEVVEEIKRIKKTDRRIIAIGTTSVRALESCYSHEINDFIPIAK
HHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCHHHHHHHHH
YVDLVIKPGYKFRVVDGLLTNFHLPKSSLLLLVSAMIGRERLLDLYKKAIKEKFRFFSYG
HHHHHCCCCCEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
DAMYISPDSLLEKNRFKL
CEEEECHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA