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The map label for this gene is vsr [H]

Identifier: 78778757

GI number: 78778757

Start: 350503

End: 350919

Strand: Direct

Name: vsr [H]

Synonym: PMT9312_0372

Alternate gene names: 78778757

Gene position: 350503-350919 (Clockwise)

Preceding gene: 78778755

Following gene: 78778758

Centisome position: 20.51

GC content: 29.74

Gene sequence:

>417_bases
ATGAGTAGTGAAGTAATGCATAAGGTTAGTGAGCAGAGATCAAGAAATATGTCTGCAATTAAATCGAAGAATACAAAGCC
TGAAATCGCGGTGAGAAAACTTCTCCATTCAATGGGATTTAGATTCAGATTGCATAGAAAAGATTTACCAGGTTCTCCTG
ATATTGTTTTGCCAAAATATAAAACAGTAATTTTTGTACATGGATGTTTCTGGCATAGGCATGAAAATTGTAAGTATGCT
TCTAATCCAAAAACAAGAAAAGAATTCTGGAAAAGTAAATTTAAAGCAAACGTAAAAAGAGACTTGGAAATTCAGGAAAA
AATAAAAAATATTGGCTGGCAATCTGTTGTTATTTGGGAATGTGAATTAACAAAAATACAGTATTTAAAAGATACTTTTT
TGAATATTAAAAATTAA

Upstream 100 bases:

>100_bases
ATAAATTACGTGGGTATGTGTGTAAACACATAATGCACGTACTGTATATAGGTACCTATAGGGTGATGGTGAGTATATTG
TTTAGAAACTATTTAGGCTG

Downstream 100 bases:

>100_bases
TTAAAAAAGTTTTTTCAAATTAGCATATAATAATTTAAAAATTTCTTCGCAAAATTGATTGATCAAGAACTCATTGATTT
TTTTAAAAACCAAATAGAAA

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: V.XorII [H]

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MSSEVMHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGFRFRLHRKDLPGSPDIVLPKYKTVIFVHGCFWHRHENCKYA
SNPKTRKEFWKSKFKANVKRDLEIQEKIKNIGWQSVVIWECELTKIQYLKDTFLNIKN

Sequences:

>Translated_138_residues
MSSEVMHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGFRFRLHRKDLPGSPDIVLPKYKTVIFVHGCFWHRHENCKYA
SNPKTRKEFWKSKFKANVKRDLEIQEKIKNIGWQSVVIWECELTKIQYLKDTFLNIKN
>Mature_137_residues
SSEVMHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGFRFRLHRKDLPGSPDIVLPKYKTVIFVHGCFWHRHENCKYAS
NPKTRKEFWKSKFKANVKRDLEIQEKIKNIGWQSVVIWECELTKIQYLKDTFLNIKN

Specific function: May nick XorII sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=123, Percent_Identity=41.4634146341463, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16460; Mature: 16329

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEVMHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGFRFRLHRKDLPGSPDIVLPKY
CCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCC
KTVIFVHGCFWHRHENCKYASNPKTRKEFWKSKFKANVKRDLEIQEKIKNIGWQSVVIWE
CEEEEEECHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEE
CELTKIQYLKDTFLNIKN
ECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSEVMHKVSEQRSRNMSAIKSKNTKPEIAVRKLLHSMGFRFRLHRKDLPGSPDIVLPKY
CHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEECCCC
KTVIFVHGCFWHRHENCKYASNPKTRKEFWKSKFKANVKRDLEIQEKIKNIGWQSVVIWE
CEEEEEECHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEE
CELTKIQYLKDTFLNIKN
ECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8078464 [H]