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The map label for this gene is def

Identifier: 78484538

GI number: 78484538

Start: 227306

End: 227815

Strand: Reverse

Name: def

Synonym: Tcr_0193

Alternate gene names: 78484538

Gene position: 227815-227306 (Counterclockwise)

Preceding gene: 78484540

Following gene: 78484537

Centisome position: 9.38

GC content: 43.53

Gene sequence:

>510_bases
ATGGATAAACTCGATATTGTTCTCTACCCTGATGAAGGATTAAGAGAGGTTTGCAAGCCCGTTCCTGAAATGACAGACGA
ACTGGATAAGCTCATTGATGAGATGTTTTACACGATGTATGACGCACCTGGGATTGGTTTAGCCGCGCCTCAGGTCGCAG
TACAACAACGCTTGATTGTGGTCGACATCTCCGAAACCAAAGATGAACCGATTGCTTTACTGAACCCGGAAATCATCAAA
ACTGCGGGCAAAATTACTTGGGAAGAAGGATGCCTTTCCATCCCGGGGATTTATGCTAAAGTCGACCGTCCATCAGACAT
TCTGGTCCGTGGCATGGATCGAGACGGAAAAACGATCGAATTTGAAGCCAATGAACTCTTAGCCGTATGCATTCAACATG
AAATTGATCATTTAAACGGCAAATTGTTTATTGACCATCTCTCTGGCTTAAAGCGGACCCGTGCAATTCAAAAATTTAAA
AAAGAAATGGCAGAACAAGCAAACTCATGA

Upstream 100 bases:

>100_bases
AAAGCCAGTCATGCAAGTTGTCTAAATCAGACATAGGTGACTCAATCCGCTATAATTTAGGGTCATTCATTCAAAACGTT
AAAAAAGTATACCTTGTATT

Downstream 100 bases:

>100_bases
CGCAGCCTCTTAGAATTATCTTCGCCGGTACCCCTGACTTTTCCGTGCCCCCCCTTAAAACCTTGATTGACTCCGAGCAT
GAGGTAGTGGCGGTTTACAC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MDKLDIVLYPDEGLREVCKPVPEMTDELDKLIDEMFYTMYDAPGIGLAAPQVAVQQRLIVVDISETKDEPIALLNPEIIK
TAGKITWEEGCLSIPGIYAKVDRPSDILVRGMDRDGKTIEFEANELLAVCIQHEIDHLNGKLFIDHLSGLKRTRAIQKFK
KEMAEQANS

Sequences:

>Translated_169_residues
MDKLDIVLYPDEGLREVCKPVPEMTDELDKLIDEMFYTMYDAPGIGLAAPQVAVQQRLIVVDISETKDEPIALLNPEIIK
TAGKITWEEGCLSIPGIYAKVDRPSDILVRGMDRDGKTIEFEANELLAVCIQHEIDHLNGKLFIDHLSGLKRTRAIQKFK
KEMAEQANS
>Mature_169_residues
MDKLDIVLYPDEGLREVCKPVPEMTDELDKLIDEMFYTMYDAPGIGLAAPQVAVQQRLIVVDISETKDEPIALLNPEIIK
TAGKITWEEGCLSIPGIYAKVDRPSDILVRGMDRDGKTIEFEANELLAVCIQHEIDHLNGKLFIDHLSGLKRTRAIQKFK
KEMAEQANS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=30.5732484076433, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1789682, Length=161, Percent_Identity=52.7950310559006, Blast_Score=176, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=31.2101910828025, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=27.3885350318471, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_THICR (Q31J84)

Other databases:

- EMBL:   CP000109
- RefSeq:   YP_390463.1
- HSSP:   P0A6K3
- ProteinModelPortal:   Q31J84
- SMR:   Q31J84
- STRING:   Q31J84
- GeneID:   3762249
- GenomeReviews:   CP000109_GR
- KEGG:   tcx:Tcr_0193
- NMPDR:   fig|317025.3.peg.166
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   RQLVDDM
- PhylomeDB:   Q31J84
- BioCyc:   TCRU317025:TCR_0193-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19010; Mature: 19010

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLDIVLYPDEGLREVCKPVPEMTDELDKLIDEMFYTMYDAPGIGLAAPQVAVQQRLIV
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCEEEE
VDISETKDEPIALLNPEIIKTAGKITWEEGCLSIPGIYAKVDRPSDILVRGMDRDGKTIE
EECCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHEEECCCCCCCEEE
FEANELLAVCIQHEIDHLNGKLFIDHLSGLKRTRAIQKFKKEMAEQANS
ECHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKLDIVLYPDEGLREVCKPVPEMTDELDKLIDEMFYTMYDAPGIGLAAPQVAVQQRLIV
CCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCEEEE
VDISETKDEPIALLNPEIIKTAGKITWEEGCLSIPGIYAKVDRPSDILVRGMDRDGKTIE
EECCCCCCCCEEEECHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHHEEECCCCCCCEEE
FEANELLAVCIQHEIDHLNGKLFIDHLSGLKRTRAIQKFKKEMAEQANS
ECHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA