Click here to switch to the map view.

The map label for this gene is ntrX [H]

Identifier: 78484533

GI number: 78484533

Start: 220822

End: 222180

Strand: Reverse

Name: ntrX [H]

Synonym: Tcr_0188

Alternate gene names: 78484533

Gene position: 222180-220822 (Counterclockwise)

Preceding gene: 78484534

Following gene: 78484532

Centisome position: 9.15

GC content: 43.78

Gene sequence:

>1359_bases
ATGAAACCAGGCAAATTACTGATCGTTGATGATGAAAAAGACATTCGTAGCCTCATGCAAGAAATTTTTTCTGAAGAAGG
TTATGAAGTTATGGTTGCCGCCAATGGGCAGCAAGCTCAAGCCATTTGGCGTGATAAAGCGCCCGATTTAATTTTTCTAG
ACATATGGATGCCCGATATTGATGGTATTTCACTTCTCAAAGAAATGATTGCCGAAAATGTTCTTGATCACAGCAGCGTC
ATAATGATGTCTGGGCACGGCACAATTGAGACCGCTATTGAAGCGACTAAGCTCGGAGCGTATGATTTTTTAGAAAAACC
ACTGTCACTCGCAAAGCTTCTGATTACAGCTGAACGGGCGATGGAACACCTTCATCTTCACAGCGAAAACCGCCATTTAA
AACAAAAATTACCGGATCAAATTTTACCGATCGGTAAAAGCAAACTCATTGTCGAACTGAGAGACTCGATTAATCGCCTC
GCAAAATACTCGATGCCCATCCTGGTTAAAGGGGAACCCGGCACGGGTAAACACCGTTTAGCCGAAGCCATTCACAAACT
GGGGGATCGCAAAGAACAGCCGTTGTTCGAAATTCATGCACCCGACTTCGATGAACAGGAGGAACTCATCCTCGGCTTGG
AAGCGGAACAAAATACCTTTCCGGGAGAGCTGATACATGCTTCTGGCGGCACCCTGATTATTTCAGATGTCGAACAACTC
AGTACCAAAGGCCAAAATTTTTTAAGCCAGCTTTTATTTGACGGCTCGTTTGTTCGTCCAGGTTCGAACAAAAAAATTGC
GCTGGATGTCCGAATTATCGCTTTAACAAAAGTCGGCTTAGACGAATTAGCGCAAAGTGGTCACTTTAGAAAAGATTTGT
TTCAGCGTTTGAATGTCATGCCTTTGGAAATCCCTTCTTTGCGCCAGCACACAGAAGACATTCCCGATTTAATTGATTAT
TTTTTGGATTACTTTCTCATGCACGAAGGACTGGAATACCGCGAATTTAATGTCGCCGCTAAAAATGTACTTCGACAATA
CAGCTGGCCTGGAAACTACCGAGAACTGCAAAATCTGGTCCAGCGTCTTCTGATTCTTGGAGAAGGAGAGGTCAATGATG
ACGAAATTCGCCGTGCACTCGAAAGCACTAAAAATGCCGATACAGTGTCCTTTTCCAGCATTAACACCACGGTTAACCTA
AAACAGGCCAAAGAGCAATTTGAAGCGGCTTATTTGAGCCAGCTGCTCCGTGAAACAGGAGGCAATGTCACGGAAACGGC
TCGATTATCAGGCGTTGAGAGAACCAACTTATATCGCAAGTTAAAAGCACTCAAAATTGACCCAAAAAATCCGAAATAA

Upstream 100 bases:

>100_bases
GACAAATTCATGTAGAATCGGAACCCGAGCAAGGAACCTGTTTTATAATAAACTTACCACAACACACTAAAGTATAGCTG
TCTAATAAAATGATCCCACT

Downstream 100 bases:

>100_bases
CTGAATAGCTTCATGCCTCGGCTTCTAAAGGTATAATCGGTTGTAATAAAAGCAAGAATTAGGGTTAATTTCAGGCTTAA
TCCAGCGTAGAATACGTAGC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKPGKLLIVDDEKDIRSLMQEIFSEEGYEVMVAANGQQAQAIWRDKAPDLIFLDIWMPDIDGISLLKEMIAENVLDHSSV
IMMSGHGTIETAIEATKLGAYDFLEKPLSLAKLLITAERAMEHLHLHSENRHLKQKLPDQILPIGKSKLIVELRDSINRL
AKYSMPILVKGEPGTGKHRLAEAIHKLGDRKEQPLFEIHAPDFDEQEELILGLEAEQNTFPGELIHASGGTLIISDVEQL
STKGQNFLSQLLFDGSFVRPGSNKKIALDVRIIALTKVGLDELAQSGHFRKDLFQRLNVMPLEIPSLRQHTEDIPDLIDY
FLDYFLMHEGLEYREFNVAAKNVLRQYSWPGNYRELQNLVQRLLILGEGEVNDDEIRRALESTKNADTVSFSSINTTVNL
KQAKEQFEAAYLSQLLRETGGNVTETARLSGVERTNLYRKLKALKIDPKNPK

Sequences:

>Translated_452_residues
MKPGKLLIVDDEKDIRSLMQEIFSEEGYEVMVAANGQQAQAIWRDKAPDLIFLDIWMPDIDGISLLKEMIAENVLDHSSV
IMMSGHGTIETAIEATKLGAYDFLEKPLSLAKLLITAERAMEHLHLHSENRHLKQKLPDQILPIGKSKLIVELRDSINRL
AKYSMPILVKGEPGTGKHRLAEAIHKLGDRKEQPLFEIHAPDFDEQEELILGLEAEQNTFPGELIHASGGTLIISDVEQL
STKGQNFLSQLLFDGSFVRPGSNKKIALDVRIIALTKVGLDELAQSGHFRKDLFQRLNVMPLEIPSLRQHTEDIPDLIDY
FLDYFLMHEGLEYREFNVAAKNVLRQYSWPGNYRELQNLVQRLLILGEGEVNDDEIRRALESTKNADTVSFSSINTTVNL
KQAKEQFEAAYLSQLLRETGGNVTETARLSGVERTNLYRKLKALKIDPKNPK
>Mature_452_residues
MKPGKLLIVDDEKDIRSLMQEIFSEEGYEVMVAANGQQAQAIWRDKAPDLIFLDIWMPDIDGISLLKEMIAENVLDHSSV
IMMSGHGTIETAIEATKLGAYDFLEKPLSLAKLLITAERAMEHLHLHSENRHLKQKLPDQILPIGKSKLIVELRDSINRL
AKYSMPILVKGEPGTGKHRLAEAIHKLGDRKEQPLFEIHAPDFDEQEELILGLEAEQNTFPGELIHASGGTLIISDVEQL
STKGQNFLSQLLFDGSFVRPGSNKKIALDVRIIALTKVGLDELAQSGHFRKDLFQRLNVMPLEIPSLRQHTEDIPDLIDY
FLDYFLMHEGLEYREFNVAAKNVLRQYSWPGNYRELQNLVQRLLILGEGEVNDDEIRRALESTKNADTVSFSSINTTVNL
KQAKEQFEAAYLSQLLRETGGNVTETARLSGVERTNLYRKLKALKIDPKNPK

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=460, Percent_Identity=32.1739130434783, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1790437, Length=449, Percent_Identity=34.5211581291759, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=31.1440677966102, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788905, Length=462, Percent_Identity=31.8181818181818, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87082152, Length=320, Percent_Identity=30.9375, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=31.8471337579618, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=32.2946175637394, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789233, Length=242, Percent_Identity=33.4710743801653, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1786524, Length=330, Percent_Identity=28.4848484848485, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789087, Length=437, Percent_Identity=26.0869565217391, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87081858, Length=299, Percent_Identity=28.7625418060201, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI87081872, Length=316, Percent_Identity=26.8987341772152, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789828, Length=238, Percent_Identity=28.9915966386555, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787229, Length=113, Percent_Identity=31.858407079646, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786784, Length=110, Percent_Identity=34.5454545454545, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789809, Length=110, Percent_Identity=28.1818181818182, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51138; Mature: 51138

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPGKLLIVDDEKDIRSLMQEIFSEEGYEVMVAANGQQAQAIWRDKAPDLIFLDIWMPDI
CCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEECCCC
DGISLLKEMIAENVLDHSSVIMMSGHGTIETAIEATKLGAYDFLEKPLSLAKLLITAERA
CHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
MEHLHLHSENRHLKQKLPDQILPIGKSKLIVELRDSINRLAKYSMPILVKGEPGTGKHRL
HHHHHHCCCCHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
AEAIHKLGDRKEQPLFEIHAPDFDEQEELILGLEAEQNTFPGELIHASGGTLIISDVEQL
HHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEECHHHH
STKGQNFLSQLLFDGSFVRPGSNKKIALDVRIIALTKVGLDELAQSGHFRKDLFQRLNVM
HHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEECCCHHHHHHCCCHHHHHHHHCCCC
PLEIPSLRQHTEDIPDLIDYFLDYFLMHEGLEYREFNVAAKNVLRQYSWPGNYRELQNLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QRLLILGEGEVNDDEIRRALESTKNADTVSFSSINTTVNLKQAKEQFEAAYLSQLLRETG
HHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHC
GNVTETARLSGVERTNLYRKLKALKIDPKNPK
CCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPGKLLIVDDEKDIRSLMQEIFSEEGYEVMVAANGQQAQAIWRDKAPDLIFLDIWMPDI
CCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEECCCC
DGISLLKEMIAENVLDHSSVIMMSGHGTIETAIEATKLGAYDFLEKPLSLAKLLITAERA
CHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
MEHLHLHSENRHLKQKLPDQILPIGKSKLIVELRDSINRLAKYSMPILVKGEPGTGKHRL
HHHHHHCCCCHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
AEAIHKLGDRKEQPLFEIHAPDFDEQEELILGLEAEQNTFPGELIHASGGTLIISDVEQL
HHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEECHHHH
STKGQNFLSQLLFDGSFVRPGSNKKIALDVRIIALTKVGLDELAQSGHFRKDLFQRLNVM
HHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEECCCHHHHHHCCCHHHHHHHHCCCC
PLEIPSLRQHTEDIPDLIDYFLDYFLMHEGLEYREFNVAAKNVLRQYSWPGNYRELQNLV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QRLLILGEGEVNDDEIRRALESTKNADTVSFSSINTTVNLKQAKEQFEAAYLSQLLRETG
HHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHC
GNVTETARLSGVERTNLYRKLKALKIDPKNPK
CCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]