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The map label for this gene is thiC

Identifier: 78484493

GI number: 78484493

Start: 179361

End: 181253

Strand: Reverse

Name: thiC

Synonym: Tcr_0148

Alternate gene names: 78484493

Gene position: 181253-179361 (Counterclockwise)

Preceding gene: 78484495

Following gene: 78484492

Centisome position: 7.47

GC content: 49.13

Gene sequence:

>1893_bases
ATGAGTACCTTTAAAAATGCACTCCCCGCATCGAACGACATCCTAACCCGTGACCCGTTACCGGCGTCCCATAAAACCTA
TATTTCAGGCGAAATTCACCCGGAAATCAAGGTGCCTATGCGAGCCATTACCCTAACCAATGGTGAAACCGTAACCGTTT
ACGACACCTCTGGCCCTTATACCGACCCGAACATAGAAATCGATGTGGCACAAGGGATTCCAACGGTTCGAAAAGACTGG
ATTGCCAACCGTAACGATGTGGAAGCGTACGAAGGCCGCATTATCGACCCAAAAGACAATGGGTATAAAAACCGCACGCA
AATGGAAACCGCCATTGCCGGCGCTTCAAGCTTGATGCGCCAACCATTGAGAGCAAAAGCCGGACAAAATGTGACTCAGC
TGCACTATGCTCGTCAAGGCATTATTACGCCTGAAATGGAGTTTATCGCTATCCGCGAAAACCAACGACGCGACATGACC
CGTCGTTATCTGAATGACCCAGAACGCGAAGCCCGCTTAAAAGGCGAAAACTTCGGTGCCAATTTACTAGAAGACATCAC
TCCTGAATTTGTTCGAAAAGAAGTGGCTGAAGGTCGTGCCGTCATTCCAGCCAACATCAATCACCCAGAATCCGAACCGA
TGATTATCGGACGAAACTTTCTGGTAAAAGTGAATGCCAACATCGGCAATTCCGCCACCACCTCTTCGATTGGCGAAGAA
GTTGAAAAAATGGTTTGGGCCACTCGCTGGGGTGCCGATACTGTCATGGACTTATCTACAGGGCAAAACATTCACACCAC
TCGTGACTGGATTTTACGAAATGCCCCCGTTCCAATTGGCACGGTACCGATTTATCAGGCACTGGAAAAGGTAAATGGCG
TTGCGGAAGACCTGACCTGGGAAATCTTCCGAGACACTTTGATTGAGCAAGCGGAACAAGGGGTCGATTACTTCACCATC
CATGCCGGCGTCCTTTTACGCTACGTGCCAATGACGGCGAAACGCGTCACCGGCATTGTCTCCCGAGGTGGGTCGATCAT
GGCGAAATGGTGTATCGCCCACCACAAAGAAAACTTTTTATACACGCACTTTGAAGATATCTGCGAAATTTTAAAACAAT
ATGATGTGTCTTTTTCTTTAGGCGATGGGCTTCGTCCTGGTTCCGTCGCGGATGCCAACGACGAAGCGCAGATTTCTGAA
CTCAAAACATTGGGCGAGCTCACTCAGATTGCTTGGAAGCACGATGTACAAGTCATCATTGAGGGGCCGGGGCATGTGCC
GATGCATATGATTAAAGAGAATATGGACAAGCAGTTGGAATATTGCCATGAAGCGCCTTTCTATACATTAGGCCCTTTAA
CAACGGATATCGCACCTGGCTACGACCACATCACCTCAGGGATTGGAGCGGCGATGATCGGCTGGTTTGGAACCGCCATG
CTGTGTTATGTAACGCCTAAAGAGCATCTTGGCTTGCCAAACCGGGATGACGTTAAAGAAGGACTGATCACCTATAAACT
GGCCGCGCATGCGGCAGACATTGCCAAAGGCCATCCCGGCGCACGCTCAAGAGATGATGCGCTCAGCCAGGCACGTTTTG
AATTCCGTTGGGAAGACCAGTTCAACCTAGGATTGGACCCTGAAAAGGCTCGCGCCTTCCATGATGAAACCTTACCAAGA
GACTCGGCTAAAGTGGCCCACTTCTGTTCGATGTGTGGCCCCAAATTCTGTTCAATGAAAATTTCACAGGAAGTGCGCGA
CTATGCGGATAAGCAAGGACTATCCACAGAAGATGCCGTCAACAAAGGGATGGAGGAAATGTCCCAAACCTTCAAAAACC
AAGGCAGTGAAGTGTACGTTAACGTCGAAGACATCACAAAAACAGGTTCTTAA

Upstream 100 bases:

>100_bases
TTCAACGGTATGACAAGTGCACAATCTTAATCACTAAGGTTTTGCCCATCTTTTTACGCTGTTTTTTTCATCCTTTGAAC
AAACGAAAAGTGAGAAAAAC

Downstream 100 bases:

>100_bases
CCCCTATTATGCACGTCGTTGCGGCCCTTGTTGTCGCAACGCGAGGAGTTGATTATGACAACACAAACCATCGGCATTGC
CGGGGCTGGACTCGTCGGTC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MSTFKNALPASNDILTRDPLPASHKTYISGEIHPEIKVPMRAITLTNGETVTVYDTSGPYTDPNIEIDVAQGIPTVRKDW
IANRNDVEAYEGRIIDPKDNGYKNRTQMETAIAGASSLMRQPLRAKAGQNVTQLHYARQGIITPEMEFIAIRENQRRDMT
RRYLNDPEREARLKGENFGANLLEDITPEFVRKEVAEGRAVIPANINHPESEPMIIGRNFLVKVNANIGNSATTSSIGEE
VEKMVWATRWGADTVMDLSTGQNIHTTRDWILRNAPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTI
HAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCIAHHKENFLYTHFEDICEILKQYDVSFSLGDGLRPGSVADANDEAQISE
LKTLGELTQIAWKHDVQVIIEGPGHVPMHMIKENMDKQLEYCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAM
LCYVTPKEHLGLPNRDDVKEGLITYKLAAHAADIAKGHPGARSRDDALSQARFEFRWEDQFNLGLDPEKARAFHDETLPR
DSAKVAHFCSMCGPKFCSMKISQEVRDYADKQGLSTEDAVNKGMEEMSQTFKNQGSEVYVNVEDITKTGS

Sequences:

>Translated_630_residues
MSTFKNALPASNDILTRDPLPASHKTYISGEIHPEIKVPMRAITLTNGETVTVYDTSGPYTDPNIEIDVAQGIPTVRKDW
IANRNDVEAYEGRIIDPKDNGYKNRTQMETAIAGASSLMRQPLRAKAGQNVTQLHYARQGIITPEMEFIAIRENQRRDMT
RRYLNDPEREARLKGENFGANLLEDITPEFVRKEVAEGRAVIPANINHPESEPMIIGRNFLVKVNANIGNSATTSSIGEE
VEKMVWATRWGADTVMDLSTGQNIHTTRDWILRNAPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTI
HAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCIAHHKENFLYTHFEDICEILKQYDVSFSLGDGLRPGSVADANDEAQISE
LKTLGELTQIAWKHDVQVIIEGPGHVPMHMIKENMDKQLEYCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAM
LCYVTPKEHLGLPNRDDVKEGLITYKLAAHAADIAKGHPGARSRDDALSQARFEFRWEDQFNLGLDPEKARAFHDETLPR
DSAKVAHFCSMCGPKFCSMKISQEVRDYADKQGLSTEDAVNKGMEEMSQTFKNQGSEVYVNVEDITKTGS
>Mature_629_residues
STFKNALPASNDILTRDPLPASHKTYISGEIHPEIKVPMRAITLTNGETVTVYDTSGPYTDPNIEIDVAQGIPTVRKDWI
ANRNDVEAYEGRIIDPKDNGYKNRTQMETAIAGASSLMRQPLRAKAGQNVTQLHYARQGIITPEMEFIAIRENQRRDMTR
RYLNDPEREARLKGENFGANLLEDITPEFVRKEVAEGRAVIPANINHPESEPMIIGRNFLVKVNANIGNSATTSSIGEEV
EKMVWATRWGADTVMDLSTGQNIHTTRDWILRNAPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTIH
AGVLLRYVPMTAKRVTGIVSRGGSIMAKWCIAHHKENFLYTHFEDICEILKQYDVSFSLGDGLRPGSVADANDEAQISEL
KTLGELTQIAWKHDVQVIIEGPGHVPMHMIKENMDKQLEYCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAML
CYVTPKEHLGLPNRDDVKEGLITYKLAAHAADIAKGHPGARSRDDALSQARFEFRWEDQFNLGLDPEKARAFHDETLPRD
SAKVAHFCSMCGPKFCSMKISQEVRDYADKQGLSTEDAVNKGMEEMSQTFKNQGSEVYVNVEDITKTGS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=626, Percent_Identity=68.8498402555911, Blast_Score=887, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_THICR (Q31JC9)

Other databases:

- EMBL:   CP000109
- RefSeq:   YP_390418.1
- STRING:   Q31JC9
- GeneID:   3762440
- GenomeReviews:   CP000109_GR
- KEGG:   tcx:Tcr_0148
- NMPDR:   fig|317025.3.peg.100
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q31JC9
- ProtClustDB:   PRK09284
- BioCyc:   TCRU317025:TCR_0148-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 70340; Mature: 70209

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: BINDING 227-227 BINDING 256-256 BINDING 285-285 BINDING 321-321 BINDING 421-421 BINDING 448-448

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKNALPASNDILTRDPLPASHKTYISGEIHPEIKVPMRAITLTNGETVTVYDTSGPY
CCCCCCCCCCCCCCEECCCCCCCCCEEECCCCCCCEECCEEEEEEECCCEEEEEECCCCC
TDPNIEIDVAQGIPTVRKDWIANRNDVEAYEGRIIDPKDNGYKNRTQMETAIAGASSLMR
CCCCEEEECCCCCCHHHHHHCCCCCCCHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
QPLRAKAGQNVTQLHYARQGIITPEMEFIAIRENQRRDMTRRYLNDPEREARLKGENFGA
HHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHCCCCCCCC
NLLEDITPEFVRKEVAEGRAVIPANINHPESEPMIIGRNFLVKVNANIGNSATTSSIGEE
HHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCCCHHHHHHHH
VEKMVWATRWGADTVMDLSTGQNIHTTRDWILRNAPVPIGTVPIYQALEKVNGVAEDLTW
HHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHH
EIFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCIAHHKENFL
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCE
YTHFEDICEILKQYDVSFSLGDGLRPGSVADANDEAQISELKTLGELTQIAWKHDVQVII
EHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
EGPGHVPMHMIKENMDKQLEYCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAM
ECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEE
LCYVTPKEHLGLPNRDDVKEGLITYKLAAHAADIAKGHPGARSRDDALSQARFEFRWEDQ
EEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEECCC
FNLGLDPEKARAFHDETLPRDSAKVAHFCSMCGPKFCSMKISQEVRDYADKQGLSTEDAV
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
NKGMEEMSQTFKNQGSEVYVNVEDITKTGS
HHHHHHHHHHHHCCCCEEEEEHHHHCCCCC
>Mature Secondary Structure 
STFKNALPASNDILTRDPLPASHKTYISGEIHPEIKVPMRAITLTNGETVTVYDTSGPY
CCCCCCCCCCCCCEECCCCCCCCCEEECCCCCCCEECCEEEEEEECCCEEEEEECCCCC
TDPNIEIDVAQGIPTVRKDWIANRNDVEAYEGRIIDPKDNGYKNRTQMETAIAGASSLMR
CCCCEEEECCCCCCHHHHHHCCCCCCCHHHCCEEECCCCCCCCCHHHHHHHHHHHHHHHH
QPLRAKAGQNVTQLHYARQGIITPEMEFIAIRENQRRDMTRRYLNDPEREARLKGENFGA
HHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHCCCCCCCC
NLLEDITPEFVRKEVAEGRAVIPANINHPESEPMIIGRNFLVKVNANIGNSATTSSIGEE
HHHHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCCCHHHHHHHH
VEKMVWATRWGADTVMDLSTGQNIHTTRDWILRNAPVPIGTVPIYQALEKVNGVAEDLTW
HHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHH
EIFRDTLIEQAEQGVDYFTIHAGVLLRYVPMTAKRVTGIVSRGGSIMAKWCIAHHKENFL
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCE
YTHFEDICEILKQYDVSFSLGDGLRPGSVADANDEAQISELKTLGELTQIAWKHDVQVII
EHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEE
EGPGHVPMHMIKENMDKQLEYCHEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFGTAM
ECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEE
LCYVTPKEHLGLPNRDDVKEGLITYKLAAHAADIAKGHPGARSRDDALSQARFEFRWEDQ
EEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEEECCC
FNLGLDPEKARAFHDETLPRDSAKVAHFCSMCGPKFCSMKISQEVRDYADKQGLSTEDAV
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
NKGMEEMSQTFKNQGSEVYVNVEDITKTGS
HHHHHHHHHHHHCCCCEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA