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The map label for this gene is thiG

Identifier: 78484490

GI number: 78484490

Start: 177199

End: 178020

Strand: Reverse

Name: thiG

Synonym: Tcr_0145

Alternate gene names: 78484490

Gene position: 178020-177199 (Counterclockwise)

Preceding gene: 78484491

Following gene: 78484489

Centisome position: 7.33

GC content: 50.0

Gene sequence:

>822_bases
ATGAACCTGACGCCTGAACACGCACAAAAATCGTCCCCCATGTCATGGACGGTGGGTGGAAAAACTCTTAACAGTCGTTT
ATTAATCGGCTCGGCCCTTTACCCATCTCCTCAAGCGATGCAAGGGGCTATTGAGGCCTCCGGTGCTGAAATCGTCACCG
TCTCATTACGTCGGCAATCGGCTGGAGATCAAGCCGGAAAAACGTTTTGGAACATGATTAAGTCTTTAAACTGCCATGTT
TTGCCCAATACCGCAGGCTGTCATTCGGCCAAAGAAGCCATCACCACGGCTCAAATGGCACGTGAAGTGTTTGAAACCAA
CTGGATCAAACTGGAAGTGATCGGCGACCAATACAACCTGCAACCTGATCCGTTCGAACTACTGAAAGCAACGGAAACTT
TAGTGAATGATGGCTTTGAAGTCTTTCCCTACACAACAGACGATTTAGTCTTAGCCCAGCGCTTAGTGGAGGCAGGGTGC
AACATTCTAATGCCTTGGGGCTCGCCGATCGGTTCGGGCAAAGGGTTGATGAACCCTTACAACCTGAACGCAATCAGACA
ACGATTTCCAGAATTAACACTGATCGTCGATGCCGGCATTGGCAAACCGTCTCACGCCGTTCAAGCACTGGAAATGGGCT
ATAATGGCATTCTGCTCAATTCTGCCGTGGCGTTATCGCCCAACCCAATCACCATGGGAAAAGCATTTAAGTCGGCCGTT
GAAGCAGGGGTTTATGCCTTTGATGCCGGCACGATGCAGGAAAGAGATTTGGCTTCGCCGTCAACCCCAGTGGTCGGCAC
GCCTTTTTGGCATCAACATTAA

Upstream 100 bases:

>100_bases
TGCAATCAATGACGCTTTCGTACCACGCAGCCAATATGAAACCACGCCGATTCAAACCGATGATCGCATCGAAATTGTCG
CCCCCATGCAAGGAGGTTAA

Downstream 100 bases:

>100_bases
CCAGGCCTGGCCGTTTTAAGGAGCCATTATGCAATTCTTATCCTGCCATTCCCCTGAAAAACCACTGGGAATTTACCCGC
TGGTGGACCGAGCCGATAAA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNLTPEHAQKSSPMSWTVGGKTLNSRLLIGSALYPSPQAMQGAIEASGAEIVTVSLRRQSAGDQAGKTFWNMIKSLNCHV
LPNTAGCHSAKEAITTAQMAREVFETNWIKLEVIGDQYNLQPDPFELLKATETLVNDGFEVFPYTTDDLVLAQRLVEAGC
NILMPWGSPIGSGKGLMNPYNLNAIRQRFPELTLIVDAGIGKPSHAVQALEMGYNGILLNSAVALSPNPITMGKAFKSAV
EAGVYAFDAGTMQERDLASPSTPVVGTPFWHQH

Sequences:

>Translated_273_residues
MNLTPEHAQKSSPMSWTVGGKTLNSRLLIGSALYPSPQAMQGAIEASGAEIVTVSLRRQSAGDQAGKTFWNMIKSLNCHV
LPNTAGCHSAKEAITTAQMAREVFETNWIKLEVIGDQYNLQPDPFELLKATETLVNDGFEVFPYTTDDLVLAQRLVEAGC
NILMPWGSPIGSGKGLMNPYNLNAIRQRFPELTLIVDAGIGKPSHAVQALEMGYNGILLNSAVALSPNPITMGKAFKSAV
EAGVYAFDAGTMQERDLASPSTPVVGTPFWHQH
>Mature_273_residues
MNLTPEHAQKSSPMSWTVGGKTLNSRLLIGSALYPSPQAMQGAIEASGAEIVTVSLRRQSAGDQAGKTFWNMIKSLNCHV
LPNTAGCHSAKEAITTAQMAREVFETNWIKLEVIGDQYNLQPDPFELLKATETLVNDGFEVFPYTTDDLVLAQRLVEAGC
NILMPWGSPIGSGKGLMNPYNLNAIRQRFPELTLIVDAGIGKPSHAVQALEMGYNGILLNSAVALSPNPITMGKAFKSAV
EAGVYAFDAGTMQERDLASPSTPVVGTPFWHQH

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=249, Percent_Identity=43.7751004016064, Blast_Score=201, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_THICR (Q31JD2)

Other databases:

- EMBL:   CP000109
- RefSeq:   YP_390415.1
- ProteinModelPortal:   Q31JD2
- SMR:   Q31JD2
- STRING:   Q31JD2
- GeneID:   3762437
- GenomeReviews:   CP000109_GR
- KEGG:   tcx:Tcr_0145
- NMPDR:   fig|317025.3.peg.97
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q31JD2
- ProtClustDB:   PRK00208
- BioCyc:   TCRU317025:TCR_0145-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 29443; Mature: 29443

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: ACT_SITE 110-110 BINDING 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTPEHAQKSSPMSWTVGGKTLNSRLLIGSALYPSPQAMQGAIEASGAEIVTVSLRRQS
CCCCCCHHHCCCCCEEEECCCEECCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCC
AGDQAGKTFWNMIKSLNCHVLPNTAGCHSAKEAITTAQMAREVFETNWIKLEVIGDQYNL
CCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
QPDPFELLKATETLVNDGFEVFPYTTDDLVLAQRLVEAGCNILMPWGSPIGSGKGLMNPY
CCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
NLNAIRQRFPELTLIVDAGIGKPSHAVQALEMGYNGILLNSAVALSPNPITMGKAFKSAV
CHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCEEEECCCCCHHHHHHHHHH
EAGVYAFDAGTMQERDLASPSTPVVGTPFWHQH
HHCEEEECCCCCCHHCCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MNLTPEHAQKSSPMSWTVGGKTLNSRLLIGSALYPSPQAMQGAIEASGAEIVTVSLRRQS
CCCCCCHHHCCCCCEEEECCCEECCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCC
AGDQAGKTFWNMIKSLNCHVLPNTAGCHSAKEAITTAQMAREVFETNWIKLEVIGDQYNL
CCCHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
QPDPFELLKATETLVNDGFEVFPYTTDDLVLAQRLVEAGCNILMPWGSPIGSGKGLMNPY
CCCHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCC
NLNAIRQRFPELTLIVDAGIGKPSHAVQALEMGYNGILLNSAVALSPNPITMGKAFKSAV
CHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEECCEEEECCCCCHHHHHHHHHH
EAGVYAFDAGTMQERDLASPSTPVVGTPFWHQH
HHCEEEECCCCCCHHCCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA