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The map label for this gene is lacA [C]

Identifier: 78358727

GI number: 78358727

Start: 3648708

End: 3649316

Strand: Direct

Name: lacA [C]

Synonym: Dde_3688

Alternate gene names: 78358727

Gene position: 3648708-3649316 (Clockwise)

Preceding gene: 78358726

Following gene: 78358728

Centisome position: 97.81

GC content: 67.0

Gene sequence:

>609_bases
ATGCTGCCGCGTCCTGAAACGCTCTGCCTGCGCGCTGCGCTGCGCGACAACGCCGAAGAACTGTGGACATGGCTTGCAGC
CGTGCCGCCCACCGCAGCGGGCACCCTGCTGCGTATGGCCATGCTGCGGCCGCTGTTCGGCAGCGGCGCGCCCCGTATGG
GAACAGGCTGCCTGCTGCGGGGCTGCCGCAACATGCACTTTGCGCAGCAGGTGCGCATCATGCCGCGCTGCAGCCTGTAC
GCCCGCGGCGGCAGCATCAGCATCGGCAGCCGCACGGCACTCAACAGCGGGGTATTTGTGGACGCCGAAGACGGCGGTAC
CATAACCATAGGCAGCGACTGCGGCATAGGGCCCGGCACCGTCATCCGTGCGGCAAACCACAGGTTCGGCAAAGCCGGTA
TTCCCATCAGCATGCAGGGGCACAGGCCGGGCCGCATAGTCATAGAGGATGACGTCTGGATAGGCGCCAACTGCACCATT
TTGCCCGATGTACGCATAGGCACCGGCGCAGTTGTGGGCGCAGGAGCAGTTGTGACGCGCGATGTGCCCCCCCATACCGT
GGCCACCGGCGTACCGGCGCGTGTCACCGGCAGCCGCTCCGACCGCTGA

Upstream 100 bases:

>100_bases
CGGCAGAACCCTGAGCGTGGACACCCCGCAGGATCTGGAAAGGGCCCGCGCCATGATGCAGCAGGATACTCTGCGCAGAG
ACTACACCGGAGCCTGACCC

Downstream 100 bases:

>100_bases
ACGCGCCATCAAACCCGTGCGCATGGCGCACAATATCTGCTTTACGGATAAACAGCATGAAAATAGCCATAACAACGTCG
TCATTCGCCCGCTTCAGCGA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MLPRPETLCLRAALRDNAEELWTWLAAVPPTAAGTLLRMAMLRPLFGSGAPRMGTGCLLRGCRNMHFAQQVRIMPRCSLY
ARGGSISIGSRTALNSGVFVDAEDGGTITIGSDCGIGPGTVIRAANHRFGKAGIPISMQGHRPGRIVIEDDVWIGANCTI
LPDVRIGTGAVVGAGAVVTRDVPPHTVATGVPARVTGSRSDR

Sequences:

>Translated_202_residues
MLPRPETLCLRAALRDNAEELWTWLAAVPPTAAGTLLRMAMLRPLFGSGAPRMGTGCLLRGCRNMHFAQQVRIMPRCSLY
ARGGSISIGSRTALNSGVFVDAEDGGTITIGSDCGIGPGTVIRAANHRFGKAGIPISMQGHRPGRIVIEDDVWIGANCTI
LPDVRIGTGAVVGAGAVVTRDVPPHTVATGVPARVTGSRSDR
>Mature_202_residues
MLPRPETLCLRAALRDNAEELWTWLAAVPPTAAGTLLRMAMLRPLFGSGAPRMGTGCLLRGCRNMHFAQQVRIMPRCSLY
ARGGSISIGSRTALNSGVFVDAEDGGTITIGSDCGIGPGTVIRAANHRFGKAGIPISMQGHRPGRIVIEDDVWIGANCTI
LPDVRIGTGAVVGAGAVVTRDVPPHTVATGVPARVTGSRSDR

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=129, Percent_Identity=34.1085271317829, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788345, Length=169, Percent_Identity=29.585798816568, Blast_Score=64, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=125, Percent_Identity=32.8, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21178; Mature: 21178

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPRPETLCLRAALRDNAEELWTWLAAVPPTAAGTLLRMAMLRPLFGSGAPRMGTGCLLR
CCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GCRNMHFAQQVRIMPRCSLYARGGSISIGSRTALNSGVFVDAEDGGTITIGSDCGIGPGT
HHCCCCHHHHHEECCCCEEEECCCEEECCCCCCCCCCEEEECCCCCEEEECCCCCCCCCC
VIRAANHRFGKAGIPISMQGHRPGRIVIEDDVWIGANCTILPDVRIGTGAVVGAGAVVTR
EEEECCCCCCCCCCCEEECCCCCCEEEEECCEEECCCCEEECCCEECCCEEEECCEEEEC
DVPPHTVATGVPARVTGSRSDR
CCCCCHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MLPRPETLCLRAALRDNAEELWTWLAAVPPTAAGTLLRMAMLRPLFGSGAPRMGTGCLLR
CCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
GCRNMHFAQQVRIMPRCSLYARGGSISIGSRTALNSGVFVDAEDGGTITIGSDCGIGPGT
HHCCCCHHHHHEECCCCEEEECCCEEECCCCCCCCCCEEEECCCCCEEEECCCCCCCCCC
VIRAANHRFGKAGIPISMQGHRPGRIVIEDDVWIGANCTILPDVRIGTGAVVGAGAVVTR
EEEECCCCCCCCCCCEEECCCCCCEEEEECCEEECCCCEEECCCEECCCEEEECCEEEEC
DVPPHTVATGVPARVTGSRSDR
CCCCCHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]