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The map label for this gene is rhmA [H]

Identifier: 78358724

GI number: 78358724

Start: 3646073

End: 3646849

Strand: Direct

Name: rhmA [H]

Synonym: Dde_3685

Alternate gene names: 78358724

Gene position: 3646073-3646849 (Clockwise)

Preceding gene: 78358723

Following gene: 78358725

Centisome position: 97.74

GC content: 64.09

Gene sequence:

>777_bases
ATGACGGAACAAACCATGCATACAGCACAGTCCATACGCGAAACCATGCAACGCGGCCTGCCCTCTGTGGGCACATGGCT
GCAGCTGCCCTCTGCCGACGTGGCCGAAATACTGGGCGCGGCAGGCTACCAGTGGGTGGCCGCCGACCTGGAGCACGGCG
CCTTCAGCCGGTCACAGCTCCCCGATGTGTTCCGTGCGGTGTCGCTGGGCGGAACGGCCCCCTTTGCCCGCGTGGCCCAC
TGCGACATGACCGGCATCAAGGCCGCGCTGGATTCCGGCGCACAGGGCCTCATTTTTCCCATGATAGAAACACGCGCCCA
GCTGGACGAAGCCATAAGCTGGGCACTCTACCCGCCTGCCGGAACACGCGGGGTGGGATTCTGCCGGGGCAACCTGTACG
GCAGAAACTTCGACCACGGCATGGCACAGGCTGCCGAAACCGTTTTTGTGGCGCAGATAGAGCACATCCGCGCCGTGGAA
AACATTGACGATATTCTGAGCCATCCGCGGCTGGACGCCATCATGGTGGGTCCCTACGACCTGTCCGGATCCATGGACTG
CACTGGCGATTTCGCCCATCCGCAATTCGCGGCCGCACTGGCCCGCATCGAGTCCGCCGCCAAAGCCCGTCAGATTCCCA
TGGGGCTGCATATCGTCTCGCCCGATCCGGCAGAGCTGGCCCGCCGCACGGCGCAAGGGTACCGCTTCATCGCTTACGGG
CTTGATGCCGTCTTTCTGTGGAATGCCGCACAGAACCCTGCCGCGGCACCCCGCTGA

Upstream 100 bases:

>100_bases
ACCTGAAACGCAGGGTGCGCACACTCATATTGACCGAAACCCAGTTCGAGGACAGCCGCGACGTGATAATGAAGCGGCCC
CACTGGAAAATTTTCTGATC

Downstream 100 bases:

>100_bases
ACCTGCAGCCGATAACGGAGTACGTCATGAAAATCACAGTATTCGGCGGTGCCGGTTTTCTCGGTTCGCATGTTTGCGAC
AAGCTGTCGGAAGCAGGCCA

Product: HPCH/HPAI aldolase family protein

Products: pyruvate; tartronate semialdehyde

Alternate protein names: KDR aldolase; 2-dehydro-3-deoxyrhamnonate aldolase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTEQTMHTAQSIRETMQRGLPSVGTWLQLPSADVAEILGAAGYQWVAADLEHGAFSRSQLPDVFRAVSLGGTAPFARVAH
CDMTGIKAALDSGAQGLIFPMIETRAQLDEAISWALYPPAGTRGVGFCRGNLYGRNFDHGMAQAAETVFVAQIEHIRAVE
NIDDILSHPRLDAIMVGPYDLSGSMDCTGDFAHPQFAAALARIESAAKARQIPMGLHIVSPDPAELARRTAQGYRFIAYG
LDAVFLWNAAQNPAAAPR

Sequences:

>Translated_258_residues
MTEQTMHTAQSIRETMQRGLPSVGTWLQLPSADVAEILGAAGYQWVAADLEHGAFSRSQLPDVFRAVSLGGTAPFARVAH
CDMTGIKAALDSGAQGLIFPMIETRAQLDEAISWALYPPAGTRGVGFCRGNLYGRNFDHGMAQAAETVFVAQIEHIRAVE
NIDDILSHPRLDAIMVGPYDLSGSMDCTGDFAHPQFAAALARIESAAKARQIPMGLHIVSPDPAELARRTAQGYRFIAYG
LDAVFLWNAAQNPAAAPR
>Mature_257_residues
TEQTMHTAQSIRETMQRGLPSVGTWLQLPSADVAEILGAAGYQWVAADLEHGAFSRSQLPDVFRAVSLGGTAPFARVAHC
DMTGIKAALDSGAQGLIFPMIETRAQLDEAISWALYPPAGTRGVGFCRGNLYGRNFDHGMAQAAETVFVAQIEHIRAVEN
IDDILSHPRLDAIMVGPYDLSGSMDCTGDFAHPQFAAALARIESAAKARQIPMGLHIVSPDPAELARRTAQGYRFIAYGL
DAVFLWNAAQNPAAAPR

Specific function: Catalyzes the reversible retro-aldol cleavage of 2-keto- 3-deoxy-L-rhamnonate (KDR) to pyruvate and lactaldehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDR aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=244, Percent_Identity=32.7868852459016, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789514, Length=233, Percent_Identity=31.3304721030043, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.20

Molecular weight: Translated: 27734; Mature: 27603

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQTMHTAQSIRETMQRGLPSVGTWLQLPSADVAEILGAAGYQWVAADLEHGAFSRSQL
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
PDVFRAVSLGGTAPFARVAHCDMTGIKAALDSGAQGLIFPMIETRAQLDEAISWALYPPA
HHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHEEEEECCC
GTRGVGFCRGNLYGRNFDHGMAQAAETVFVAQIEHIRAVENIDDILSHPRLDAIMVGPYD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
LSGSMDCTGDFAHPQFAAALARIESAAKARQIPMGLHIVSPDPAELARRTAQGYRFIAYG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCEEEEEC
LDAVFLWNAAQNPAAAPR
CCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TEQTMHTAQSIRETMQRGLPSVGTWLQLPSADVAEILGAAGYQWVAADLEHGAFSRSQL
CCHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
PDVFRAVSLGGTAPFARVAHCDMTGIKAALDSGAQGLIFPMIETRAQLDEAISWALYPPA
HHHHHHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHEEEEECCC
GTRGVGFCRGNLYGRNFDHGMAQAAETVFVAQIEHIRAVENIDDILSHPRLDAIMVGPYD
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
LSGSMDCTGDFAHPQFAAALARIESAAKARQIPMGLHIVSPDPAELARRTAQGYRFIAYG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCEEEEEC
LDAVFLWNAAQNPAAAPR
CCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-dehydro-3-deoxy-D-glucarate

Specific reaction: 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA