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The map label for this gene is amtB [C]

Identifier: 78358531

GI number: 78358531

Start: 3452034

End: 3453419

Strand: Direct

Name: amtB [C]

Synonym: Dde_3492

Alternate gene names: 78358531

Gene position: 3452034-3453419 (Clockwise)

Preceding gene: 78358530

Following gene: 78358532

Centisome position: 92.54

GC content: 61.11

Gene sequence:

>1386_bases
ATGCACGCACCCGTATCGTCACCCCGCCATCCGGCAAAACGTTTTGCCCGCACGGCGGCTCTCTGGCTGGTTGTGGCAAC
TCTGCTCCACGCTTCACCCGCACTGGCGGAAGATGCTCCGGCAATGCTCAGCCAGCAGCATGGCAACATCCTCTGGACGC
TCATCGCCGCCATTCTGGTCATGCTCATGCAGGCCGGATTTGCCATGGTGGAATGCGGATTCACCCGTGCCAAAAACGCG
GGCAACATCCTCATGAAAAACTTTGTGGACTTTGCGGCGGGTTCACTGGTGTTCTTTCTGGTCGGCTATGCCTTCATGTT
CGGCGCCGACGCAGGCGGTTTCATCGGCACATCGGGATTTGCCCTTGCCCATGTGGACCCCGCCACAGAAGGCGGCATGT
GGGATCTGACCTTCTGGTTCTTCCAGTCGGTGTTCGCGGCCACGGCGGCCACCATCGTATCCGGCGGCATTGCCGAACGC
ACCAGATTTGCCAGCTACATCATTGTCTCCGTACTGGTGACAGGCATCATCTACCCCGTATCGGGCCACTGGGCATGGGG
CGGACTGGCCGGAAACGCCGGATGGCTTGAAGGGCTGGGCTTTGCCGACTTCGCAGGATCGACGGTTGTCCATTCCGTGG
GCGGCTGGGTTGCCCTTGCCGGGGCCATCGTGGTGGGTCCCCGTGCGGGCAAATACGGACCCGACGGCTCCGTGCGGGCC
ATACCGGGCCACAACATTCCGCTGGCGGCTCTGGGTGTGTTCATACTGTGGTTCGGCTGGTTCGGGTTCAACCCCGGCTC
CACCACCACCGCAGACGGCACCATAGGCTATATCGCCGTGAACACCAGTCTGGCAGCCTGTGCCGGTACCGTTACCGCCA
TGCTCACCGCATGGATACGGTTCGGCAAACCCGATGCATCCATAACCATGAACGGCGCTCTGGCAGGTCTGGTGGGCATC
ACGGCAGGCTGTTACGAGCTTTCCCCTTCCGGCGCCATCATCGTGGGGATCATCTGCGGCTTTGTGGTCGTGCTCTCCAT
TGAGTTTCTGGACAAGATTCTGAAGATCGACGACCCCGTGGGCGCGGTTTCCGTGCACGGCGTGTGCGGCGCCATAGGTA
CGCTGATGGTGGGGCTGCTGGCGCATCCCTCTTACGGCGGATCCACAGGCCTTGTATACGGCGGCGATGCTTCTCTGCTC
ATGGTACAGGCTCTCGGTGTGGCGGTGGTATTCGCCTGGGCCTTCGGCATGGGGCTGGTGGTGTTCCGCATTGTGGCCGC
AGTGGCGGGCCTGCGTGTCTCTCCCGAAGAAGAGCTCAAAGGTCTGGATATCACGGAACACGGTTCCGAATCGTACAACG
GATTTCAGATTTTCACCGTGGAATAG

Upstream 100 bases:

>100_bases
AAAACATATAATAACAGCATGTTATCATATTTGGCCCTCCTTTTGCTCTTACCGGAACATCGCCATTAGAACGGGCCCTG
CCCAAAAGGAGGATTATTCC

Downstream 100 bases:

>100_bases
CAGGCCAAAGGAGAACCATCATGAAATTGCTTATCGCCTATATACGCCCTGAAAAGCTCTCGACGGTGAAAAAGGCTCTT
TATGCCAGAGGCATATACGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MHAPVSSPRHPAKRFARTAALWLVVATLLHASPALAEDAPAMLSQQHGNILWTLIAAILVMLMQAGFAMVECGFTRAKNA
GNILMKNFVDFAAGSLVFFLVGYAFMFGADAGGFIGTSGFALAHVDPATEGGMWDLTFWFFQSVFAATAATIVSGGIAER
TRFASYIIVSVLVTGIIYPVSGHWAWGGLAGNAGWLEGLGFADFAGSTVVHSVGGWVALAGAIVVGPRAGKYGPDGSVRA
IPGHNIPLAALGVFILWFGWFGFNPGSTTTADGTIGYIAVNTSLAACAGTVTAMLTAWIRFGKPDASITMNGALAGLVGI
TAGCYELSPSGAIIVGIICGFVVVLSIEFLDKILKIDDPVGAVSVHGVCGAIGTLMVGLLAHPSYGGSTGLVYGGDASLL
MVQALGVAVVFAWAFGMGLVVFRIVAAVAGLRVSPEEELKGLDITEHGSESYNGFQIFTVE

Sequences:

>Translated_461_residues
MHAPVSSPRHPAKRFARTAALWLVVATLLHASPALAEDAPAMLSQQHGNILWTLIAAILVMLMQAGFAMVECGFTRAKNA
GNILMKNFVDFAAGSLVFFLVGYAFMFGADAGGFIGTSGFALAHVDPATEGGMWDLTFWFFQSVFAATAATIVSGGIAER
TRFASYIIVSVLVTGIIYPVSGHWAWGGLAGNAGWLEGLGFADFAGSTVVHSVGGWVALAGAIVVGPRAGKYGPDGSVRA
IPGHNIPLAALGVFILWFGWFGFNPGSTTTADGTIGYIAVNTSLAACAGTVTAMLTAWIRFGKPDASITMNGALAGLVGI
TAGCYELSPSGAIIVGIICGFVVVLSIEFLDKILKIDDPVGAVSVHGVCGAIGTLMVGLLAHPSYGGSTGLVYGGDASLL
MVQALGVAVVFAWAFGMGLVVFRIVAAVAGLRVSPEEELKGLDITEHGSESYNGFQIFTVE
>Mature_461_residues
MHAPVSSPRHPAKRFARTAALWLVVATLLHASPALAEDAPAMLSQQHGNILWTLIAAILVMLMQAGFAMVECGFTRAKNA
GNILMKNFVDFAAGSLVFFLVGYAFMFGADAGGFIGTSGFALAHVDPATEGGMWDLTFWFFQSVFAATAATIVSGGIAER
TRFASYIIVSVLVTGIIYPVSGHWAWGGLAGNAGWLEGLGFADFAGSTVVHSVGGWVALAGAIVVGPRAGKYGPDGSVRA
IPGHNIPLAALGVFILWFGWFGFNPGSTTTADGTIGYIAVNTSLAACAGTVTAMLTAWIRFGKPDASITMNGALAGLVGI
TAGCYELSPSGAIIVGIICGFVVVLSIEFLDKILKIDDPVGAVSVHGVCGAIGTLMVGLLAHPSYGGSTGLVYGGDASLL
MVQALGVAVVFAWAFGMGLVVFRIVAAVAGLRVSPEEELKGLDITEHGSESYNGFQIFTVE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=425, Percent_Identity=32.4705882352941, Blast_Score=204, Evalue=9e-54,
Organism=Caenorhabditis elegans, GI17550182, Length=437, Percent_Identity=35.2402745995423, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17550180, Length=424, Percent_Identity=34.9056603773585, Blast_Score=206, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=32.2429906542056, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI133931100, Length=445, Percent_Identity=30.7865168539326, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17563228, Length=185, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=28.9044289044289, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6321559, Length=419, Percent_Identity=26.7303102625298, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325396, Length=428, Percent_Identity=26.1682242990654, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=35.609756097561, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47604; Mature: 47604

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAPVSSPRHPAKRFARTAALWLVVATLLHASPALAEDAPAMLSQQHGNILWTLIAAILV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
MLMQAGFAMVECGFTRAKNAGNILMKNFVDFAAGSLVFFLVGYAFMFGADAGGFIGTSGF
HHHHCCHHEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
ALAHVDPATEGGMWDLTFWFFQSVFAATAATIVSGGIAERTRFASYIIVSVLVTGIIYPV
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECC
SGHWAWGGLAGNAGWLEGLGFADFAGSTVVHSVGGWVALAGAIVVGPRAGKYGPDGSVRA
CCCEEECCCCCCCHHHHCCCCHHHCCCHHHHHHCHHHHHHHHHEECCCCCCCCCCCCEEE
IPGHNIPLAALGVFILWFGWFGFNPGSTTTADGTIGYIAVNTSLAACAGTVTAMLTAWIR
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
FGKPDASITMNGALAGLVGITAGCYELSPSGAIIVGIICGFVVVLSIEFLDKILKIDDPV
CCCCCCCEEECHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAVSVHGVCGAIGTLMVGLLAHPSYGGSTGLVYGGDASLLMVQALGVAVVFAWAFGMGLV
CCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
VFRIVAAVAGLRVSPEEELKGLDITEHGSESYNGFQIFTVE
HHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MHAPVSSPRHPAKRFARTAALWLVVATLLHASPALAEDAPAMLSQQHGNILWTLIAAILV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
MLMQAGFAMVECGFTRAKNAGNILMKNFVDFAAGSLVFFLVGYAFMFGADAGGFIGTSGF
HHHHCCHHEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
ALAHVDPATEGGMWDLTFWFFQSVFAATAATIVSGGIAERTRFASYIIVSVLVTGIIYPV
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEECC
SGHWAWGGLAGNAGWLEGLGFADFAGSTVVHSVGGWVALAGAIVVGPRAGKYGPDGSVRA
CCCEEECCCCCCCHHHHCCCCHHHCCCHHHHHHCHHHHHHHHHEECCCCCCCCCCCCEEE
IPGHNIPLAALGVFILWFGWFGFNPGSTTTADGTIGYIAVNTSLAACAGTVTAMLTAWIR
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
FGKPDASITMNGALAGLVGITAGCYELSPSGAIIVGIICGFVVVLSIEFLDKILKIDDPV
CCCCCCCEEECHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAVSVHGVCGAIGTLMVGLLAHPSYGGSTGLVYGGDASLLMVQALGVAVVFAWAFGMGLV
CCEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
VFRIVAAVAGLRVSPEEELKGLDITEHGSESYNGFQIFTVE
HHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]