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The map label for this gene is glnB [H]

Identifier: 78358532

GI number: 78358532

Start: 3453440

End: 3453766

Strand: Direct

Name: glnB [H]

Synonym: Dde_3493

Alternate gene names: 78358532

Gene position: 3453440-3453766 (Clockwise)

Preceding gene: 78358531

Following gene: 78358536

Centisome position: 92.58

GC content: 56.27

Gene sequence:

>327_bases
ATGAAATTGCTTATCGCCTATATACGCCCTGAAAAGCTCTCGACGGTGAAAAAGGCTCTTTATGCCAGAGGCATATACGC
CATGTCCGTGACCAACGCATTGGGCAGCGGACAGCAGAAAGGCTTTACCGAAACCTATCGCGGCATTGTGACGGAAGTGA
ATCTGCTGAAAAAGGTCCGCATAGAGGTGGGGGTACCCGCGGACAAAGCGGAAGACGCTCTGGCGGCGCTGCAGACAGGT
GCCCACACCGGACGTGAGGGAGACGGCATAATCTTTGTGCAGGACGTGGCCAGAACAGTACGGGTACGGACCGGCGAAGC
CCCCTGA

Upstream 100 bases:

>100_bases
AAGAAGAGCTCAAAGGTCTGGATATCACGGAACACGGTTCCGAATCGTACAACGGATTTCAGATTTTCACCGTGGAATAG
CAGGCCAAAGGAGAACCATC

Downstream 100 bases:

>100_bases
ACATACCCCGCGGCCGCAAGGCCGCCGACTACAGGAACAACTGCCGGCTCATGAACGGTTCCGGCGGACTCCATCCGCAA
TCCCTGCAGGAAAAGGCAGC

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 108

Protein sequence:

>108_residues
MKLLIAYIRPEKLSTVKKALYARGIYAMSVTNALGSGQQKGFTETYRGIVTEVNLLKKVRIEVGVPADKAEDALAALQTG
AHTGREGDGIIFVQDVARTVRVRTGEAP

Sequences:

>Translated_108_residues
MKLLIAYIRPEKLSTVKKALYARGIYAMSVTNALGSGQQKGFTETYRGIVTEVNLLKKVRIEVGVPADKAEDALAALQTG
AHTGREGDGIIFVQDVARTVRVRTGEAP
>Mature_108_residues
MKLLIAYIRPEKLSTVKKALYARGIYAMSVTNALGSGQQKGFTETYRGIVTEVNLLKKVRIEVGVPADKAEDALAALQTG
AHTGREGDGIIFVQDVARTVRVRTGEAP

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=106, Percent_Identity=49.0566037735849, Blast_Score=94, Evalue=2e-21,
Organism=Escherichia coli, GI1786655, Length=107, Percent_Identity=43.9252336448598, Blast_Score=93, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 11660; Mature: 11660

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLIAYIRPEKLSTVKKALYARGIYAMSVTNALGSGQQKGFTETYRGIVTEVNLLKKVR
CCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IEVGVPADKAEDALAALQTGAHTGREGDGIIFVQDVARTVRVRTGEAP
HHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLLIAYIRPEKLSTVKKALYARGIYAMSVTNALGSGQQKGFTETYRGIVTEVNLLKKVR
CCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
IEVGVPADKAEDALAALQTGAHTGREGDGIIFVQDVARTVRVRTGEAP
HHCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1702507; 2878685 [H]