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The map label for this gene is opuD [H]

Identifier: 78358345

GI number: 78358345

Start: 3290827

End: 3292464

Strand: Direct

Name: opuD [H]

Synonym: Dde_3305

Alternate gene names: 78358345

Gene position: 3290827-3292464 (Clockwise)

Preceding gene: 78358343

Following gene: 78358346

Centisome position: 88.22

GC content: 55.92

Gene sequence:

>1638_bases
GTGAACGAACAAAGCAACAAAAACTCCACTCCTGAAGCAGATACGAAACCGAGCTGGTTTCATCTGGAAGTACATCCGTG
GGTCTTTTTCGGCTCCGCGGGGCTTATCTTCCTCTTCGTCGGCATCACCTTAGTTTTCCAGCATTTTCTTGGCGACGTAT
TCCAGACGATGCAGTCCTACATGTCCACCTACACGGGCTGGTTCTTCATCGCCACGATGAACATTGTACTGCTGTTTGTT
CTGCTGCTTACTGTCAGCCGCTACGGAGACATCCGACTGGGCGGTCCCGATGCCGAGCCAGAATTTTCCACCATGGCGTG
GTTTGCCATGCTGTTTTCCGCCGGCATGGGCATCGGCCTGCTGTTTTACGGAGTGGCAGAACCCATGTTCCACTATGTTG
CCAATCCGCTGACAGAACCGGGAACAACGGAAGCCGCCCGCAAAGCCATGGACATCACGTTTCTGCACTGGGGCCTGCAT
CCGTGGGCTGTGTATAGCGTTGTCGCCCTGTCGCTGGCCTTTTTTTCATTCAACAAAGGGCTGCCCCTTTCCATCCGGAC
CGCATTCTACCCCGTGCTGGGCGAGCGGATTTACGGCCCTGTGGGCAACGCCATCGATATCATGGCCACGGTGGCAACCC
TGTTCGGGGTGGCCACGTCGCTGGGTCTGGGAGTCAAGCAGGTCAATGCGGGACTGGACCATCTGTGGGGTATCGGACAG
TCCACGGGCATTCAGGTCATTCTTATTGCGGCCATCACCGCTGTTGCCACATGGAGTGTCGTCGCAGGTCTTGACAAAGG
CATCCGCCGGCTGAGTGAGATCAACATCGTTCTTGCCGGCATGCTGGCGCTGTTTGTGCTTTTTCTCGGTCCCACGCTTT
TCATCCTTAACGCCACACTGGAAAACATAGGCTATTACATTCAGTTTCTGCCGCAGCTTTCCACATGGAACGAGACATAT
GAACATACCGAGTGGCAGCACGGCTGGACCATTTTTTACTGGGCCTGGTGGATCGCCTGGTCACCTTTTGTAGGCATGTT
CATCGCCCGGGTATCTTTCGGGCGCACCATCCGCGAATTCATCATGGGAGTCATGCTGGTACCCACGTTCATCACGTTTC
TGTGGATCACCATCTTCGGCAACACAGCACTGAACATCGAAATGTTCGGGGCAGGCGGCATTGCCACCGCCGTGCAGGAA
AACCTGCCGGTATCCCTTTTCGTCATGCTGGAAAACTTCCCGCTCAGCTCTGTCACCTGCTTTCTGGGCATCGTGGTGGT
CATAACCTTCTTTGTGACCTCTTCCGACTCCGGCTCCATGGTCATTGACATCATAACTTCGGGCGGAGACCCCAACCCGC
CTGTTCTTTCACGCCTTTTCTGGGCGTTGCTCGAAGGCGTTGTGGCCGCGGTGCTGCTGATGGGCGGCGGACTGGTGGCG
CTGCAGACAGCCACCATCACCACGGGGCTGCCTTTTGCGGTCATCCTGCTCGGCATGTGCTATTCACTGCACAAAGGGCT
CAAAGAATACTCCGGCCCGCAGGAATTTACGCTGGGTATTCCCAAGCAGCAGGTACGCTTCCGGGTGAAGCGCAAACCTG
TACTGGACAGAACCTTCGGGAGGCGCAAGTTCTGGTAG

Upstream 100 bases:

>100_bases
ACGGGATACGTACCGGCTTATTCCGGTTTACGCCGGGCAGGATGCGCTCCGTGCTGAACGGAGCGTGCCGGACGGCCGCC
CCCTACACAAGGATGAGACT

Downstream 100 bases:

>100_bases
CAGCCCTGTTTCCTCAGCCTGACAAGGAGAACCATCATGCGCAGAGTTATCGTTTTTGTCGCAGCGGTGCTTATTCTGGC
AGCCGGTCCTGTCCGCGCGG

Product: choline/carnitine/betaine transport

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNEQSNKNSTPEADTKPSWFHLEVHPWVFFGSAGLIFLFVGITLVFQHFLGDVFQTMQSYMSTYTGWFFIATMNIVLLFV
LLLTVSRYGDIRLGGPDAEPEFSTMAWFAMLFSAGMGIGLLFYGVAEPMFHYVANPLTEPGTTEAARKAMDITFLHWGLH
PWAVYSVVALSLAFFSFNKGLPLSIRTAFYPVLGERIYGPVGNAIDIMATVATLFGVATSLGLGVKQVNAGLDHLWGIGQ
STGIQVILIAAITAVATWSVVAGLDKGIRRLSEINIVLAGMLALFVLFLGPTLFILNATLENIGYYIQFLPQLSTWNETY
EHTEWQHGWTIFYWAWWIAWSPFVGMFIARVSFGRTIREFIMGVMLVPTFITFLWITIFGNTALNIEMFGAGGIATAVQE
NLPVSLFVMLENFPLSSVTCFLGIVVVITFFVTSSDSGSMVIDIITSGGDPNPPVLSRLFWALLEGVVAAVLLMGGGLVA
LQTATITTGLPFAVILLGMCYSLHKGLKEYSGPQEFTLGIPKQQVRFRVKRKPVLDRTFGRRKFW

Sequences:

>Translated_545_residues
MNEQSNKNSTPEADTKPSWFHLEVHPWVFFGSAGLIFLFVGITLVFQHFLGDVFQTMQSYMSTYTGWFFIATMNIVLLFV
LLLTVSRYGDIRLGGPDAEPEFSTMAWFAMLFSAGMGIGLLFYGVAEPMFHYVANPLTEPGTTEAARKAMDITFLHWGLH
PWAVYSVVALSLAFFSFNKGLPLSIRTAFYPVLGERIYGPVGNAIDIMATVATLFGVATSLGLGVKQVNAGLDHLWGIGQ
STGIQVILIAAITAVATWSVVAGLDKGIRRLSEINIVLAGMLALFVLFLGPTLFILNATLENIGYYIQFLPQLSTWNETY
EHTEWQHGWTIFYWAWWIAWSPFVGMFIARVSFGRTIREFIMGVMLVPTFITFLWITIFGNTALNIEMFGAGGIATAVQE
NLPVSLFVMLENFPLSSVTCFLGIVVVITFFVTSSDSGSMVIDIITSGGDPNPPVLSRLFWALLEGVVAAVLLMGGGLVA
LQTATITTGLPFAVILLGMCYSLHKGLKEYSGPQEFTLGIPKQQVRFRVKRKPVLDRTFGRRKFW
>Mature_545_residues
MNEQSNKNSTPEADTKPSWFHLEVHPWVFFGSAGLIFLFVGITLVFQHFLGDVFQTMQSYMSTYTGWFFIATMNIVLLFV
LLLTVSRYGDIRLGGPDAEPEFSTMAWFAMLFSAGMGIGLLFYGVAEPMFHYVANPLTEPGTTEAARKAMDITFLHWGLH
PWAVYSVVALSLAFFSFNKGLPLSIRTAFYPVLGERIYGPVGNAIDIMATVATLFGVATSLGLGVKQVNAGLDHLWGIGQ
STGIQVILIAAITAVATWSVVAGLDKGIRRLSEINIVLAGMLALFVLFLGPTLFILNATLENIGYYIQFLPQLSTWNETY
EHTEWQHGWTIFYWAWWIAWSPFVGMFIARVSFGRTIREFIMGVMLVPTFITFLWITIFGNTALNIEMFGAGGIATAVQE
NLPVSLFVMLENFPLSSVTCFLGIVVVITFFVTSSDSGSMVIDIITSGGDPNPPVLSRLFWALLEGVVAAVLLMGGGLVA
LQTATITTGLPFAVILLGMCYSLHKGLKEYSGPQEFTLGIPKQQVRFRVKRKPVLDRTFGRRKFW

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=499, Percent_Identity=39.8797595190381, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1786224, Length=504, Percent_Identity=29.3650793650794, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788102, Length=484, Percent_Identity=30.5785123966942, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 60283; Mature: 60283

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQSNKNSTPEADTKPSWFHLEVHPWVFFGSAGLIFLFVGITLVFQHFLGDVFQTMQSY
CCCCCCCCCCCCCCCCCCEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSTYTGWFFIATMNIVLLFVLLLTVSRYGDIRLGGPDAEPEFSTMAWFAMLFSAGMGIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHH
LFYGVAEPMFHYVANPLTEPGTTEAARKAMDITFLHWGLHPWAVYSVVALSLAFFSFNKG
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCC
LPLSIRTAFYPVLGERIYGPVGNAIDIMATVATLFGVATSLGLGVKQVNAGLDHLWGIGQ
CCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHCCCC
STGIQVILIAAITAVATWSVVAGLDKGIRRLSEINIVLAGMLALFVLFLGPTLFILNATL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENIGYYIQFLPQLSTWNETYEHTEWQHGWTIFYWAWWIAWSPFVGMFIARVSFGRTIREF
HHHHHHHHHHHCHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMGVMLVPTFITFLWITIFGNTALNIEMFGAGGIATAVQENLPVSLFVMLENFPLSSVTC
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCEEEEEEEECCCHHHHHH
FLGIVVVITFFVTSSDSGSMVIDIITSGGDPNPPVLSRLFWALLEGVVAAVLLMGGGLVA
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
LQTATITTGLPFAVILLGMCYSLHKGLKEYSGPQEFTLGIPKQQVRFRVKRKPVLDRTFG
EEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCHHHHCC
RRKFW
CCCCC
>Mature Secondary Structure
MNEQSNKNSTPEADTKPSWFHLEVHPWVFFGSAGLIFLFVGITLVFQHFLGDVFQTMQSY
CCCCCCCCCCCCCCCCCCEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSTYTGWFFIATMNIVLLFVLLLTVSRYGDIRLGGPDAEPEFSTMAWFAMLFSAGMGIGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHHH
LFYGVAEPMFHYVANPLTEPGTTEAARKAMDITFLHWGLHPWAVYSVVALSLAFFSFNKG
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCC
LPLSIRTAFYPVLGERIYGPVGNAIDIMATVATLFGVATSLGLGVKQVNAGLDHLWGIGQ
CCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHCCCC
STGIQVILIAAITAVATWSVVAGLDKGIRRLSEINIVLAGMLALFVLFLGPTLFILNATL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENIGYYIQFLPQLSTWNETYEHTEWQHGWTIFYWAWWIAWSPFVGMFIARVSFGRTIREF
HHHHHHHHHHHCHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IMGVMLVPTFITFLWITIFGNTALNIEMFGAGGIATAVQENLPVSLFVMLENFPLSSVTC
HHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHCCCCEEEEEEEECCCHHHHHH
FLGIVVVITFFVTSSDSGSMVIDIITSGGDPNPPVLSRLFWALLEGVVAAVLLMGGGLVA
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
LQTATITTGLPFAVILLGMCYSLHKGLKEYSGPQEFTLGIPKQQVRFRVKRKPVLDRTFG
EEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCHHHHCC
RRKFW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]