Click here to switch to the map view.

The map label for this gene is ackA

Identifier: 78358282

GI number: 78358282

Start: 3237856

End: 3239064

Strand: Direct

Name: ackA

Synonym: Dde_3242

Alternate gene names: 78358282

Gene position: 3237856-3239064 (Clockwise)

Preceding gene: 78358281

Following gene: 78358283

Centisome position: 86.8

GC content: 56.41

Gene sequence:

>1209_bases
ATGAACGTACTGGTTATCAACGCTGGCAGCTCTTCGTGCAAATACCAGCTGATCAATATGGAATCCGAAGGCGTGCTGTG
CGCCGGTCTGGTGGAACGCATCGGCGAAGCCACCGGCAAGCTGGCCCACAAAATCGCCCCCGATACCGACTCCGAAGAAA
AGATCGTGCTGGAACAGCCCTTCCCCAATCATGTGGAAGCCATGAAAAAGGTTGTCGAGCTGATCACCGACCCTGAAAAG
GGCGTCATCAAAGACAAGTCCGAAATCTACGCCATCGGCCACCGCGTGCTGCTGGGCGGCGAAGAAATCAAGGAATCCGT
CAAGATTGACGAATGGGCAAAGGGCGTCATCCGCGATTACATTCCGCTGGGCCCCCTGCACAACCCTGCCAACCTTGCCG
GTATCGAAGTGGCCGAGGAACTGTTCCCCGGCGTGCCTTCCGTGGGCGTTTTCGACACCGAATTCCATCAGACCATGCCC
GCCAAGGCATACCTGTACCCCCTGCCGCTGGAACTGTACGAAGAGATGAAAATCCGCCGCTACGGCTTTCACGGCACCTC
GCACCGCTACGTCACCAAAAAGACAGCGGAATTTCTGGGCAAGCCGCTGGATGAAGTGAATATCATCACCTGCCATCTGG
GCAACGGCTGCTCCATGGCTGCCGTTAAAAACGGCAAGTGCGTAGACACCACCATGGGCATCACCCCGCTGGAAGGCCTG
ATGATGGGCACCCGCTGCGGCGATATCGACCCGGCCATCGTGCCCTTCCTGATGGAAAAGAAAGGTCTGACCACCGCAGA
AGCCGACACGCTGATGAACAAGCAGTCCGGCCTTAAGGGTGTATGCGGCATGAATGATATGCGCGACATACACGCCGCCG
TGGAAAAAGGTGACGAAAAAGCCAAACTGGCGCTGGACATGTTTGTCTACCGCATCAAAAAATACATCGGTGCCTTTTAT
GCAGCCCTCGGCCGTGTGGATGCCGTGGTATTCACCGCCGGTATCGGTGAAAACGACGACATCGTCCGTGCCGAAGTATG
CGAAAACATGGACGTGTTCGGCATCGCTCTGGATGCGGAAAAGAACAAGATCCGCTCCGGCGAACCCCGCAACATCGCCG
CAGAAAACAGCAAAGTGGCCGTGCTGGTGGTACCCACCAACGAAGAGCTGGAAATTGCTCAGGCTGCTGTGAACGTGCTC
AAAGGCTAA

Upstream 100 bases:

>100_bases
GCCTGACATCGTCAACACCGTCGCCATCACCGCCATTCAGGCTCAGGCGGAAAAAGGCCTTCGCTAGGCCATTTCATAGA
GTAAAAGAGGATATAGCAGA

Downstream 100 bases:

>100_bases
CAACCGGACCTGTGCCGCAAGGCATGACATAGACGCGGGGGCGGGGCTCCGGCAGCTCCGCCCCCTTTTTTCTTCCGGCA
ACGGATGCAGAGAGACACAT

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MNVLVINAGSSSCKYQLINMESEGVLCAGLVERIGEATGKLAHKIAPDTDSEEKIVLEQPFPNHVEAMKKVVELITDPEK
GVIKDKSEIYAIGHRVLLGGEEIKESVKIDEWAKGVIRDYIPLGPLHNPANLAGIEVAEELFPGVPSVGVFDTEFHQTMP
AKAYLYPLPLELYEEMKIRRYGFHGTSHRYVTKKTAEFLGKPLDEVNIITCHLGNGCSMAAVKNGKCVDTTMGITPLEGL
MMGTRCGDIDPAIVPFLMEKKGLTTAEADTLMNKQSGLKGVCGMNDMRDIHAAVEKGDEKAKLALDMFVYRIKKYIGAFY
AALGRVDAVVFTAGIGENDDIVRAEVCENMDVFGIALDAEKNKIRSGEPRNIAAENSKVAVLVVPTNEELEIAQAAVNVL
KG

Sequences:

>Translated_402_residues
MNVLVINAGSSSCKYQLINMESEGVLCAGLVERIGEATGKLAHKIAPDTDSEEKIVLEQPFPNHVEAMKKVVELITDPEK
GVIKDKSEIYAIGHRVLLGGEEIKESVKIDEWAKGVIRDYIPLGPLHNPANLAGIEVAEELFPGVPSVGVFDTEFHQTMP
AKAYLYPLPLELYEEMKIRRYGFHGTSHRYVTKKTAEFLGKPLDEVNIITCHLGNGCSMAAVKNGKCVDTTMGITPLEGL
MMGTRCGDIDPAIVPFLMEKKGLTTAEADTLMNKQSGLKGVCGMNDMRDIHAAVEKGDEKAKLALDMFVYRIKKYIGAFY
AALGRVDAVVFTAGIGENDDIVRAEVCENMDVFGIALDAEKNKIRSGEPRNIAAENSKVAVLVVPTNEELEIAQAAVNVL
KG
>Mature_402_residues
MNVLVINAGSSSCKYQLINMESEGVLCAGLVERIGEATGKLAHKIAPDTDSEEKIVLEQPFPNHVEAMKKVVELITDPEK
GVIKDKSEIYAIGHRVLLGGEEIKESVKIDEWAKGVIRDYIPLGPLHNPANLAGIEVAEELFPGVPSVGVFDTEFHQTMP
AKAYLYPLPLELYEEMKIRRYGFHGTSHRYVTKKTAEFLGKPLDEVNIITCHLGNGCSMAAVKNGKCVDTTMGITPLEGL
MMGTRCGDIDPAIVPFLMEKKGLTTAEADTLMNKQSGLKGVCGMNDMRDIHAAVEKGDEKAKLALDMFVYRIKKYIGAFY
AALGRVDAVVFTAGIGENDDIVRAEVCENMDVFGIALDAEKNKIRSGEPRNIAAENSKVAVLVVPTNEELEIAQAAVNVL
KG

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI145693184, Length=402, Percent_Identity=42.0398009950249, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1788633, Length=401, Percent_Identity=46.1346633416459, Blast_Score=311, Evalue=4e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACKA_DESDG (Q30WB0)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389731.1
- ProteinModelPortal:   Q30WB0
- SMR:   Q30WB0
- STRING:   Q30WB0
- GeneID:   3758217
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3242
- NMPDR:   fig|207559.3.peg.3006
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   TDEEYMI
- ProtClustDB:   PRK00180
- BioCyc:   DDES207559:DDE_3242-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43764; Mature: 43764

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVINAGSSSCKYQLINMESEGVLCAGLVERIGEATGKLAHKIAPDTDSEEKIVLEQP
CEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
FPNHVEAMKKVVELITDPEKGVIKDKSEIYAIGHRVLLGGEEIKESVKIDEWAKGVIRDY
CCHHHHHHHHHHHHHCCCCCCCCCCCHHEEEECCEEEECCHHHHHHCCHHHHHHHHHHHC
IPLGPLHNPANLAGIEVAEELFPGVPSVGVFDTEFHQTMPAKAYLYPLPLELYEEMKIRR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHH
YGFHGTSHRYVTKKTAEFLGKPLDEVNIITCHLGNGCSMAAVKNGKCVDTTMGITPLEGL
CCCCCCCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCEEEEECCCCCHHHH
MMGTRCGDIDPAIVPFLMEKKGLTTAEADTLMNKQSGLKGVCGMNDMRDIHAAVEKGDEK
HCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCH
AKLALDMFVYRIKKYIGAFYAALGRVDAVVFTAGIGENDDIVRAEVCENMDVFGIALDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEEHHHHHCCCCEEEEEEECC
KNKIRSGEPRNIAAENSKVAVLVVPTNEELEIAQAAVNVLKG
CCCCCCCCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNVLVINAGSSSCKYQLINMESEGVLCAGLVERIGEATGKLAHKIAPDTDSEEKIVLEQP
CEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECC
FPNHVEAMKKVVELITDPEKGVIKDKSEIYAIGHRVLLGGEEIKESVKIDEWAKGVIRDY
CCHHHHHHHHHHHHHCCCCCCCCCCCHHEEEECCEEEECCHHHHHHCCHHHHHHHHHHHC
IPLGPLHNPANLAGIEVAEELFPGVPSVGVFDTEFHQTMPAKAYLYPLPLELYEEMKIRR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEECCCHHHHHHHHHHH
YGFHGTSHRYVTKKTAEFLGKPLDEVNIITCHLGNGCSMAAVKNGKCVDTTMGITPLEGL
CCCCCCCCCEEHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCEEEEECCCCCHHHH
MMGTRCGDIDPAIVPFLMEKKGLTTAEADTLMNKQSGLKGVCGMNDMRDIHAAVEKGDEK
HCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCH
AKLALDMFVYRIKKYIGAFYAALGRVDAVVFTAGIGENDDIVRAEVCENMDVFGIALDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCEEHHHHHCCCCEEEEEEECC
KNKIRSGEPRNIAAENSKVAVLVVPTNEELEIAQAAVNVLKG
CCCCCCCCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA