Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 78358260

GI number: 78358260

Start: 3210575

End: 3211078

Strand: Direct

Name: leuD

Synonym: Dde_3220

Alternate gene names: 78358260

Gene position: 3210575-3211078 (Clockwise)

Preceding gene: 78358259

Following gene: 78358261

Centisome position: 86.07

GC content: 60.32

Gene sequence:

>504_bases
ATGCAATACAACGGCACCGCCCGCAAGGTGGGCGACCACATAGACACAGACGCAATCATTCCCGCACGGTTTCTGGTTAC
CACAGACCCCGCGCAGCTGGGCGCCAACTGCATGGAAGGCCTTGAACACGGGTGGGTGTCCCGCGTGCAGCAGGGTGACA
TTATGGTGGGCGGCAAAAACTTCGGCTGCGGTTCGTCGCGCGAGCATGCCCCCATTGCCATACTCGGTGCGGGCATGCCT
GTTGTGGTGGCGCACAGCTTTGCACGCATATTCTACCGCAACGGCTTCAACATGGGACTCATCCTCATAGAAATCGGCGA
CGACGCGGACAGAATACACGACGGCGACGTTCTGAGCGTGGATGTGGAAAACGGGCGCATCACCAATCACACCACGGGCG
ATGTGATCAGCTGCCCGCCTCTGCCGCCCTTTATGCACGACATTCTGGACAAAGGCGGACTGGTGCCCTACGTGCGCGAA
CGTCTGGCCGCCGGAGACGCATAA

Upstream 100 bases:

>100_bases
GAATCAGAGGTGTATCTTTCCAACCCGTCCGTGGCCGCGGCTTCCGCCGTGGCAGGCGAAATAGCCGACCCGCGCGGCCT
GTAGCAGCAAGGAGACAACG

Downstream 100 bases:

>100_bases
ATGCAGGCCGCCATGGAGTGCCTGCCCTGCATGATGGCGCAGGCTGTAAGGGTAGCCCGGCTGGCCGCCCCGGACGATGA
AGCCGTGCAGCAGAACGTGG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MQYNGTARKVGDHIDTDAIIPARFLVTTDPAQLGANCMEGLEHGWVSRVQQGDIMVGGKNFGCGSSREHAPIAILGAGMP
VVVAHSFARIFYRNGFNMGLILIEIGDDADRIHDGDVLSVDVENGRITNHTTGDVISCPPLPPFMHDILDKGGLVPYVRE
RLAAGDA

Sequences:

>Translated_167_residues
MQYNGTARKVGDHIDTDAIIPARFLVTTDPAQLGANCMEGLEHGWVSRVQQGDIMVGGKNFGCGSSREHAPIAILGAGMP
VVVAHSFARIFYRNGFNMGLILIEIGDDADRIHDGDVLSVDVENGRITNHTTGDVISCPPLPPFMHDILDKGGLVPYVRE
RLAAGDA
>Mature_167_residues
MQYNGTARKVGDHIDTDAIIPARFLVTTDPAQLGANCMEGLEHGWVSRVQQGDIMVGGKNFGCGSSREHAPIAILGAGMP
VVVAHSFARIFYRNGFNMGLILIEIGDDADRIHDGDVLSVDVENGRITNHTTGDVISCPPLPPFMHDILDKGGLVPYVRE
RLAAGDA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=108, Percent_Identity=39.8148148148148, Blast_Score=75, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320440, Length=97, Percent_Identity=41.2371134020619, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=165, Percent_Identity=32.7272727272727, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_DESDG (Q30WD2)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389709.1
- ProteinModelPortal:   Q30WD2
- SMR:   Q30WD2
- STRING:   Q30WD2
- GeneID:   3758195
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3220
- NMPDR:   fig|207559.3.peg.2943
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   PFPEFMQ
- ProtClustDB:   PRK00439
- BioCyc:   DDES207559:DDE_3220-MONOMER
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 17907; Mature: 17907

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYNGTARKVGDHIDTDAIIPARFLVTTDPAQLGANCMEGLEHGWVSRVQQGDIMVGGKN
CCCCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
FGCGSSREHAPIAILGAGMPVVVAHSFARIFYRNGFNMGLILIEIGDDADRIHDGDVLSV
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCCCCCEEEE
DVENGRITNHTTGDVISCPPLPPFMHDILDKGGLVPYVRERLAAGDA
ECCCCEECCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MQYNGTARKVGDHIDTDAIIPARFLVTTDPAQLGANCMEGLEHGWVSRVQQGDIMVGGKN
CCCCCCHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
FGCGSSREHAPIAILGAGMPVVVAHSFARIFYRNGFNMGLILIEIGDDADRIHDGDVLSV
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHCCCCCEEEE
DVENGRITNHTTGDVISCPPLPPFMHDILDKGGLVPYVRERLAAGDA
ECCCCEECCCCCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA