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The map label for this gene is leuC

Identifier: 78358259

GI number: 78358259

Start: 3209302

End: 3210558

Strand: Direct

Name: leuC

Synonym: Dde_3219

Alternate gene names: 78358259

Gene position: 3209302-3210558 (Clockwise)

Preceding gene: 78358258

Following gene: 78358260

Centisome position: 86.03

GC content: 62.29

Gene sequence:

>1257_bases
ATGGCTCATACGCTTGCGCAGAAGATACTGCAGAAGCACACAGACCAGCAGGTGGGCGAAGCAGGGCAGATAGTGCGCTG
CCGCGTTTCCATGGCGCTGGCCAACGACATCACCGCTCCGCTGGCCATCAAGTCCTTCAGGGCAATGGGTGCCGGCAAGG
TGTTTGACAAAGACAGAGTGGCCCTTGTCATGGACCACTTCACCCCGCAGAAAGATATCGATTCGGCCATTCAGGTAAAA
AACACGCGCGAATTTGCCGCGCAGATGGGCATAACCCATTATTACGAAGGCGGCGAGGCCGGTGTGGAACATGCCCTGCT
GCCCGAACTCGGCCTTGTGGGTCCGGGCGATATTGTGGTGGGCGCAGACAGCCACACCTGCACTTACGGCGGGCTGGGCG
CATTTGCCACCGGTCTCGGCTCCACCGACGTGGCCGGAGCCATGGCGCTGGGCGAAACCTGGTTCAAGGTGCCGCCGAGC
ATACGCGTTTCCTACCGCGGCACCATGCCTGCCCACGTGGGAGCAAAAGACCTTGTGTTGCAGCTCATCGGGCATATCGG
CGTTGACGGAGCGCTGTACAAGGCGCTGGAATTTGACGGCCCCGTGGTGGATGCCATGAGCGTGGAAGGCCGTATGACCA
TTGCCAATATGGCCATTGAAGCCGGCGGCAAATGCGGTCTGTTTCCCTCCGATGACCTGACGCTGGCCTACACCGCCGCC
AGAGGCCGCAACGACGAAAAGCTGAGCGCCGATCAGGACGCCGTATACGAACGCAGTCTGTCATTCGACGTTTCAGACCT
TGCACCGCAGATAGCCTGCCCTCACCTGCCCGAAAATGTTCGCCCGGTTACCGAAGTGCAGGGCGTGCAGATACATCAGG
CTGTCATCGGCTCGTGCACCAACGGCCGCATAAGCGACCTGCGCGAGGCGGCAGCCGTGCTGCGCGGGCGCAAGGTGCAC
AAAAGCGTACGCTGCATCGTGCTGCCTGCCACCCCGGGCATCTGGAAGCAGGCCCTGCGCGAAGGGCTTATCGAAACCTT
CATGGATGCAGGCTGCATAGTGGGCCCCGCCACCTGCGGCCCCTGTCTGGGCGGGCACATGGGGATTCTTGCCGACGGAG
AACGCGCCATAGCCACCACCAACCGCAACTTCCGCGGCCGCATGGGCAGCCTCGAATCAGAGGTGTATCTTTCCAACCCG
TCCGTGGCCGCGGCTTCCGCCGTGGCAGGCGAAATAGCCGACCCGCGCGGCCTGTAG

Upstream 100 bases:

>100_bases
GGTAGGCCGCGGTGCCGATGCTGATATCATCACAGCCAGTGCAAAAGCGTATCTCAATGCGCTGAACAGGCTTGCCAAAA
AAGAACAGGAGGACAAATAG

Downstream 100 bases:

>100_bases
CAGCAAGGAGACAACGATGCAATACAACGGCACCGCCCGCAAGGTGGGCGACCACATAGACACAGACGCAATCATTCCCG
CACGGTTTCTGGTTACCACA

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAHTLAQKILQKHTDQQVGEAGQIVRCRVSMALANDITAPLAIKSFRAMGAGKVFDKDRVALVMDHFTPQKDIDSAIQVK
NTREFAAQMGITHYYEGGEAGVEHALLPELGLVGPGDIVVGADSHTCTYGGLGAFATGLGSTDVAGAMALGETWFKVPPS
IRVSYRGTMPAHVGAKDLVLQLIGHIGVDGALYKALEFDGPVVDAMSVEGRMTIANMAIEAGGKCGLFPSDDLTLAYTAA
RGRNDEKLSADQDAVYERSLSFDVSDLAPQIACPHLPENVRPVTEVQGVQIHQAVIGSCTNGRISDLREAAAVLRGRKVH
KSVRCIVLPATPGIWKQALREGLIETFMDAGCIVGPATCGPCLGGHMGILADGERAIATTNRNFRGRMGSLESEVYLSNP
SVAAASAVAGEIADPRGL

Sequences:

>Translated_418_residues
MAHTLAQKILQKHTDQQVGEAGQIVRCRVSMALANDITAPLAIKSFRAMGAGKVFDKDRVALVMDHFTPQKDIDSAIQVK
NTREFAAQMGITHYYEGGEAGVEHALLPELGLVGPGDIVVGADSHTCTYGGLGAFATGLGSTDVAGAMALGETWFKVPPS
IRVSYRGTMPAHVGAKDLVLQLIGHIGVDGALYKALEFDGPVVDAMSVEGRMTIANMAIEAGGKCGLFPSDDLTLAYTAA
RGRNDEKLSADQDAVYERSLSFDVSDLAPQIACPHLPENVRPVTEVQGVQIHQAVIGSCTNGRISDLREAAAVLRGRKVH
KSVRCIVLPATPGIWKQALREGLIETFMDAGCIVGPATCGPCLGGHMGILADGERAIATTNRNFRGRMGSLESEVYLSNP
SVAAASAVAGEIADPRGL
>Mature_417_residues
AHTLAQKILQKHTDQQVGEAGQIVRCRVSMALANDITAPLAIKSFRAMGAGKVFDKDRVALVMDHFTPQKDIDSAIQVKN
TREFAAQMGITHYYEGGEAGVEHALLPELGLVGPGDIVVGADSHTCTYGGLGAFATGLGSTDVAGAMALGETWFKVPPSI
RVSYRGTMPAHVGAKDLVLQLIGHIGVDGALYKALEFDGPVVDAMSVEGRMTIANMAIEAGGKCGLFPSDDLTLAYTAAR
GRNDEKLSADQDAVYERSLSFDVSDLAPQIACPHLPENVRPVTEVQGVQIHQAVIGSCTNGRISDLREAAAVLRGRKVHK
SVRCIVLPATPGIWKQALREGLIETFMDAGCIVGPATCGPCLGGHMGILADGERAIATTNRNFRGRMGSLESEVYLSNPS
VAAASAVAGEIADPRGL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=446, Percent_Identity=29.1479820627803, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI8659555, Length=371, Percent_Identity=25.3369272237197, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=22.976501305483, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI1786259, Length=473, Percent_Identity=31.0782241014799, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=26.4957264957265, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI2367097, Length=332, Percent_Identity=26.2048192771084, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25149337, Length=447, Percent_Identity=27.5167785234899, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=351, Percent_Identity=27.3504273504274, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=285, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17568399, Length=374, Percent_Identity=24.0641711229947, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320440, Length=446, Percent_Identity=34.5291479820628, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=31.6348195329087, Blast_Score=205, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6323335, Length=448, Percent_Identity=30.5803571428571, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322261, Length=459, Percent_Identity=26.1437908496732, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571643, Length=445, Percent_Identity=26.5168539325843, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281365315, Length=446, Percent_Identity=27.3542600896861, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17864292, Length=446, Percent_Identity=27.3542600896861, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161076999, Length=410, Percent_Identity=27.5609756097561, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=25.3369272237197, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=26.4150943396226, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_DESDG (Q30WD3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389708.1
- ProteinModelPortal:   Q30WD3
- SMR:   Q30WD3
- STRING:   Q30WD3
- GeneID:   3758194
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_3219
- NMPDR:   fig|207559.3.peg.2942
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   PENTKTI
- ProtClustDB:   PRK00402
- BioCyc:   DDES207559:DDE_3219-MONOMER
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- InterPro:   IPR011823
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086
- TIGRFAMs:   TIGR02083

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 44027; Mature: 43896

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHTLAQKILQKHTDQQVGEAGQIVRCRVSMALANDITAPLAIKSFRAMGAGKVFDKDRV
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCE
ALVMDHFTPQKDIDSAIQVKNTREFAAQMGITHYYEGGEAGVEHALLPELGLVGPGDIVV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHCCCCCCCCCCCEEE
GADSHTCTYGGLGAFATGLGSTDVAGAMALGETWFKVPPSIRVSYRGTMPAHVGAKDLVL
ECCCCEEECCCHHHHHHCCCCCHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCHHHHHH
QLIGHIGVDGALYKALEFDGPVVDAMSVEGRMTIANMAIEAGGKCGLFPSDDLTLAYTAA
HHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHCCCCCCCCCCCCCEEEEEEC
RGRNDEKLSADQDAVYERSLSFDVSDLAPQIACPHLPENVRPVTEVQGVQIHQAVIGSCT
CCCCCCCCCCCHHHHHHHHCCCCHHHHCHHHCCCCCCCCCCCHHHHCCCEEHHHHHCCCC
NGRISDLREAAAVLRGRKVHKSVRCIVLPATPGIWKQALREGLIETFMDAGCIVGPATCG
CCHHHHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEECCCCCC
PCLGGHMGILADGERAIATTNRNFRGRMGSLESEVYLSNPSVAAASAVAGEIADPRGL
HHHCCCCCEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AHTLAQKILQKHTDQQVGEAGQIVRCRVSMALANDITAPLAIKSFRAMGAGKVFDKDRV
CHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCE
ALVMDHFTPQKDIDSAIQVKNTREFAAQMGITHYYEGGEAGVEHALLPELGLVGPGDIVV
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHCCCCCCCCCCCEEE
GADSHTCTYGGLGAFATGLGSTDVAGAMALGETWFKVPPSIRVSYRGTMPAHVGAKDLVL
ECCCCEEECCCHHHHHHCCCCCHHHHHHHHCCHHHCCCCCEEEEEECCCCCCCCHHHHHH
QLIGHIGVDGALYKALEFDGPVVDAMSVEGRMTIANMAIEAGGKCGLFPSDDLTLAYTAA
HHHHHCCCCHHHHHHHHCCCCEEEEEECCCCEEEEHHHHHCCCCCCCCCCCCCEEEEEEC
RGRNDEKLSADQDAVYERSLSFDVSDLAPQIACPHLPENVRPVTEVQGVQIHQAVIGSCT
CCCCCCCCCCCHHHHHHHHCCCCHHHHCHHHCCCCCCCCCCCHHHHCCCEEHHHHHCCCC
NGRISDLREAAAVLRGRKVHKSVRCIVLPATPGIWKQALREGLIETFMDAGCIVGPATCG
CCHHHHHHHHHHHHHCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEECCCCCC
PCLGGHMGILADGERAIATTNRNFRGRMGSLESEVYLSNPSVAAASAVAGEIADPRGL
HHHCCCCCEEECCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA