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The map label for this gene is nifU [H]

Identifier: 78358118

GI number: 78358118

Start: 3068055

End: 3068897

Strand: Direct

Name: nifU [H]

Synonym: Dde_3078

Alternate gene names: 78358118

Gene position: 3068055-3068897 (Clockwise)

Preceding gene: 78358117

Following gene: 78358119

Centisome position: 82.25

GC content: 56.94

Gene sequence:

>843_bases
ATGTGGGATTACACCGACAAGGTTCGAGACCACTTTCTCAACCCGCGTAACGCGGGTGCCATAGAAGACGCCAACGCCGT
GGGAGAGGTAGGAAGCCTTGCCTGCGGCGATGCTTTGAAACTATACCTTAAAATTGACGATCAGGAACGCATTGTAGATG
CGAAATTCCAGACGTTCGGCTGCGCCAGCGCCATTGCCTCTTCCTCCGCCCTGACGGAGATGATCAAAGGCATGACGGTG
GATGAAGCCATGAAGGTGACCAACAAGGACATCACCGCTTATCTGGGCGGGCTTCCCCAGCAGAAAATGCACTGCTCCGT
CATGGGGCAGGAGGCGCTGGAAGCCGCCATTAAAAATTACCGCGGCGAAGAAGTGGAACAGCACCACCACGAAGGGCAGC
TGGTCTGTAAGTGCTTCGGCGTGACCGACGAGCAGATTATCCGCAGCATACGCGAAAACAAACTGACCTCGGTGGAAGAG
GTGACCCACTTCACCAAGGCCGGCGGCGGCTGCGGCGACTGCCTGAACGACATACAGAAGCTGCTGGATGAAGAGCTGGG
CAAAACAGCCGCCTGCGAAGTTCCCGAACCGCAGCCGCGTACCCTCAGCAATGTCCAGCGCATGCAGCTGGTGGTAAAAA
CGCTGGAAGAAGATATACGCCCCAGCCTGCAGAAGGACGGCGGCGACATAGAGCTTGTGGACATTGACGGCAAGGAAGTC
AAAGTCGCCCTGCGCGGCATGTGTACCCACTGCCCTTCCAGCCAGCTCACGCTGACCAATTTTGTGCAACGTACGCTGCG
CGAACACGTGGAACCGGACATCACGGTGAAGGAGGTTTCATAA

Upstream 100 bases:

>100_bases
AAAAAAAAACGCTCCGTGCACTTCCCCCGCGCCCGTTTATTATTATATGTGAAGGGCGCAACTGTTCTGCGCACCCATCA
GCAGTGACCGGAGGATCATC

Downstream 100 bases:

>100_bases
ATGCGCACCATATATATGGACAACAACGCCACCACCGCCGTTGACCCCGCCGTGCTGGACGAAATGCTGCCGTACCTGAA
AGACCTGTACGGCAACCCTT

Product: Fe-S cluster assembly protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MWDYTDKVRDHFLNPRNAGAIEDANAVGEVGSLACGDALKLYLKIDDQERIVDAKFQTFGCASAIASSSALTEMIKGMTV
DEAMKVTNKDITAYLGGLPQQKMHCSVMGQEALEAAIKNYRGEEVEQHHHEGQLVCKCFGVTDEQIIRSIRENKLTSVEE
VTHFTKAGGGCGDCLNDIQKLLDEELGKTAACEVPEPQPRTLSNVQRMQLVVKTLEEDIRPSLQKDGGDIELVDIDGKEV
KVALRGMCTHCPSSQLTLTNFVQRTLREHVEPDITVKEVS

Sequences:

>Translated_280_residues
MWDYTDKVRDHFLNPRNAGAIEDANAVGEVGSLACGDALKLYLKIDDQERIVDAKFQTFGCASAIASSSALTEMIKGMTV
DEAMKVTNKDITAYLGGLPQQKMHCSVMGQEALEAAIKNYRGEEVEQHHHEGQLVCKCFGVTDEQIIRSIRENKLTSVEE
VTHFTKAGGGCGDCLNDIQKLLDEELGKTAACEVPEPQPRTLSNVQRMQLVVKTLEEDIRPSLQKDGGDIELVDIDGKEV
KVALRGMCTHCPSSQLTLTNFVQRTLREHVEPDITVKEVS
>Mature_280_residues
MWDYTDKVRDHFLNPRNAGAIEDANAVGEVGSLACGDALKLYLKIDDQERIVDAKFQTFGCASAIASSSALTEMIKGMTV
DEAMKVTNKDITAYLGGLPQQKMHCSVMGQEALEAAIKNYRGEEVEQHHHEGQLVCKCFGVTDEQIIRSIRENKLTSVEE
VTHFTKAGGGCGDCLNDIQKLLDEELGKTAACEVPEPQPRTLSNVQRMQLVVKTLEEDIRPSLQKDGGDIELVDIDGKEV
KVALRGMCTHCPSSQLTLTNFVQRTLREHVEPDITVKEVS

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=121, Percent_Identity=47.9338842975207, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI24307953, Length=122, Percent_Identity=46.7213114754098, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788878, Length=121, Percent_Identity=47.1074380165289, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17543474, Length=127, Percent_Identity=46.4566929133858, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324800, Length=122, Percent_Identity=47.5409836065574, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325122, Length=125, Percent_Identity=44, Blast_Score=116, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355597, Length=121, Percent_Identity=47.9338842975207, Blast_Score=120, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 30877; Mature: 30877

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDYTDKVRDHFLNPRNAGAIEDANAVGEVGSLACGDALKLYLKIDDQERIVDAKFQTFG
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHCCEEEEEEEECCHHHHHHHHHHHHH
CASAIASSSALTEMIKGMTVDEAMKVTNKDITAYLGGLPQQKMHCSVMGQEALEAAIKNY
HHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
RGEEVEQHHHEGQLVCKCFGVTDEQIIRSIRENKLTSVEEVTHFTKAGGGCGDCLNDIQK
CCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHH
LLDEELGKTAACEVPEPQPRTLSNVQRMQLVVKTLEEDIRPSLQKDGGDIELVDIDGKEV
HHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEECCCHHH
KVALRGMCTHCPSSQLTLTNFVQRTLREHVEPDITVKEVS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MWDYTDKVRDHFLNPRNAGAIEDANAVGEVGSLACGDALKLYLKIDDQERIVDAKFQTFG
CCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHCCEEEEEEEECCHHHHHHHHHHHHH
CASAIASSSALTEMIKGMTVDEAMKVTNKDITAYLGGLPQQKMHCSVMGQEALEAAIKNY
HHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHC
RGEEVEQHHHEGQLVCKCFGVTDEQIIRSIRENKLTSVEEVTHFTKAGGGCGDCLNDIQK
CCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHH
LLDEELGKTAACEVPEPQPRTLSNVQRMQLVVKTLEEDIRPSLQKDGGDIELVDIDGKEV
HHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCEEEEECCCHHH
KVALRGMCTHCPSSQLTLTNFVQRTLREHVEPDITVKEVS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]