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The map label for this gene is nifS1 [H]

Identifier: 78358119

GI number: 78358119

Start: 3068898

End: 3070088

Strand: Direct

Name: nifS1 [H]

Synonym: Dde_3079

Alternate gene names: 78358119

Gene position: 3068898-3070088 (Clockwise)

Preceding gene: 78358118

Following gene: 78358120

Centisome position: 82.27

GC content: 59.11

Gene sequence:

>1191_bases
ATGCGCACCATATATATGGACAACAACGCCACCACCGCCGTTGACCCCGCCGTGCTGGACGAAATGCTGCCGTACCTGAA
AGACCTGTACGGCAACCCTTCCAGCATGCACACCTTCGGCGGGCAGGTAGGCCGTCAGGTGGCCGAGGCCCGCGAACGCG
TGGCGCGTGCGCTGGGCGCCCAGCCCGAAGAAATTCTCTTCACCTCGTGCGGCACCGAAAGCGACAACACTGCCATCATG
TCCGCCCTGCAGGCCCAGCCTGAAAAAAAACATATCATCACCACCCGCGTGGAACACCCCGCTGTGCTTAATCTGGTACA
GCATCTGGAAAAAAAGGGGTATGAAGCCACGTATCTGAGTGTGGATGAAAAAGGACGTCTTGATCTGGATGAGCTGCGCG
GTGCCCTGCGCAAAGACACGGCCATCGTTTCCGTGATGTACGCAAACAATGAGACCGGCACCATTTTTCCCATCAAGGAA
ATTGCAGCCATCGTCAAGGAAAAGGGCGCGCTTTTTCACACAGACGCAGTGCAGGCCGTCGGCAAACTGCATATAAACGT
GCAGGAAGTGCCTGTGGATTTCCTTGCGCTTTCCGGCCACAAGCTGCACGCCCCCAAAGGTGTGGGAGCGCTGTACGTGC
GCCGCGGCACTCCGTACCGGCCTTTTCTGCGGGGCGGACATCAGGAACGCGGCCGCCGTGCCGGCACGGAGAATGTGGCC
GGCATCATTGCGCTGGGCAAAGCCTGCGAGCTGGCTTCCCTGAATATGGAAACCGAACGCACCACCGTCAAGGCGCTGCG
CGACAGGCTGGAGAACGGCCTGCTCAATGCCATACCCGAAGCCATCATCAACGGTGATACGGACAACAGACTGCCCAATA
CAACCAATATCGCCTTCAAATATGTCGAAGGCGAAGCCATTCTGCTGCTGATGGACGGACACGGCATATGTGCCAGCTCC
GGTTCGGCATGCACTTCAGGCAGCCTTGAGCCTTCGCACGTACTGCGTGCCATGGGAGTGCCGTTCACCTATGCCCACGG
CTCCATCCGGTTCAGCCTGAGCCGGTTCAACACACAGGCGGATGTGGACTACGTGGTGGACGAACTGCCGCGCATCATCG
CCCGTCTGCGTGAAATATCGCCCTACCGCAGCGGTGAAGACGCGCAGGCAAAGCCTGCGTGTACCTGCTGA

Upstream 100 bases:

>100_bases
TACCCACTGCCCTTCCAGCCAGCTCACGCTGACCAATTTTGTGCAACGTACGCTGCGCGAACACGTGGAACCGGACATCA
CGGTGAAGGAGGTTTCATAA

Downstream 100 bases:

>100_bases
AACGGCCCGGGCATACATGCATCCGAGGGGCCGGAAACGGACGCACTGCGCGTCCGGTTCCGGCCTTTTTTTTACAGCAA
ATGGAGGAAACACCGTGAAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MRTIYMDNNATTAVDPAVLDEMLPYLKDLYGNPSSMHTFGGQVGRQVAEARERVARALGAQPEEILFTSCGTESDNTAIM
SALQAQPEKKHIITTRVEHPAVLNLVQHLEKKGYEATYLSVDEKGRLDLDELRGALRKDTAIVSVMYANNETGTIFPIKE
IAAIVKEKGALFHTDAVQAVGKLHINVQEVPVDFLALSGHKLHAPKGVGALYVRRGTPYRPFLRGGHQERGRRAGTENVA
GIIALGKACELASLNMETERTTVKALRDRLENGLLNAIPEAIINGDTDNRLPNTTNIAFKYVEGEAILLLMDGHGICASS
GSACTSGSLEPSHVLRAMGVPFTYAHGSIRFSLSRFNTQADVDYVVDELPRIIARLREISPYRSGEDAQAKPACTC

Sequences:

>Translated_396_residues
MRTIYMDNNATTAVDPAVLDEMLPYLKDLYGNPSSMHTFGGQVGRQVAEARERVARALGAQPEEILFTSCGTESDNTAIM
SALQAQPEKKHIITTRVEHPAVLNLVQHLEKKGYEATYLSVDEKGRLDLDELRGALRKDTAIVSVMYANNETGTIFPIKE
IAAIVKEKGALFHTDAVQAVGKLHINVQEVPVDFLALSGHKLHAPKGVGALYVRRGTPYRPFLRGGHQERGRRAGTENVA
GIIALGKACELASLNMETERTTVKALRDRLENGLLNAIPEAIINGDTDNRLPNTTNIAFKYVEGEAILLLMDGHGICASS
GSACTSGSLEPSHVLRAMGVPFTYAHGSIRFSLSRFNTQADVDYVVDELPRIIARLREISPYRSGEDAQAKPACTC
>Mature_396_residues
MRTIYMDNNATTAVDPAVLDEMLPYLKDLYGNPSSMHTFGGQVGRQVAEARERVARALGAQPEEILFTSCGTESDNTAIM
SALQAQPEKKHIITTRVEHPAVLNLVQHLEKKGYEATYLSVDEKGRLDLDELRGALRKDTAIVSVMYANNETGTIFPIKE
IAAIVKEKGALFHTDAVQAVGKLHINVQEVPVDFLALSGHKLHAPKGVGALYVRRGTPYRPFLRGGHQERGRRAGTENVA
GIIALGKACELASLNMETERTTVKALRDRLENGLLNAIPEAIINGDTDNRLPNTTNIAFKYVEGEAILLLMDGHGICASS
GSACTSGSLEPSHVLRAMGVPFTYAHGSIRFSLSRFNTQADVDYVVDELPRIIARLREISPYRSGEDAQAKPACTC

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=45.7583547557841, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=36.3855421686747, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI48994898, Length=394, Percent_Identity=45.1776649746193, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789175, Length=400, Percent_Identity=26.5, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=387, Percent_Identity=44.4444444444444, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17533177, Length=322, Percent_Identity=33.8509316770186, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=47.3958333333333, Blast_Score=343, Evalue=2e-95,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=44.7916666666667, Blast_Score=327, Evalue=8e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43150; Mature: 43150

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIYMDNNATTAVDPAVLDEMLPYLKDLYGNPSSMHTFGGQVGRQVAEARERVARALGA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
QPEEILFTSCGTESDNTAIMSALQAQPEKKHIITTRVEHPAVLNLVQHLEKKGYEATYLS
CCHHEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
VDEKGRLDLDELRGALRKDTAIVSVMYANNETGTIFPIKEIAAIVKEKGALFHTDAVQAV
ECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEHHHHHHH
GKLHINVQEVPVDFLALSGHKLHAPKGVGALYVRRGTPYRPFLRGGHQERGRRAGTENVA
HHHEEEHHHCCEEEEEECCCEEECCCCCCEEEEECCCCCCHHHCCCCHHHHHHCCCCHHH
GIIALGKACELASLNMETERTTVKALRDRLENGLLNAIPEAIINGDTDNRLPNTTNIAFK
HHEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEE
YVEGEAILLLMDGHGICASSGSACTSGSLEPSHVLRAMGVPFTYAHGSIRFSLSRFNTQA
EECCCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCEEEEEEECCCCCC
DVDYVVDELPRIIARLREISPYRSGEDAQAKPACTC
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTIYMDNNATTAVDPAVLDEMLPYLKDLYGNPSSMHTFGGQVGRQVAEARERVARALGA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
QPEEILFTSCGTESDNTAIMSALQAQPEKKHIITTRVEHPAVLNLVQHLEKKGYEATYLS
CCHHEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
VDEKGRLDLDELRGALRKDTAIVSVMYANNETGTIFPIKEIAAIVKEKGALFHTDAVQAV
ECCCCCCCHHHHHHHHHCCCEEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEHHHHHHH
GKLHINVQEVPVDFLALSGHKLHAPKGVGALYVRRGTPYRPFLRGGHQERGRRAGTENVA
HHHEEEHHHCCEEEEEECCCEEECCCCCCEEEEECCCCCCHHHCCCCHHHHHHCCCCHHH
GIIALGKACELASLNMETERTTVKALRDRLENGLLNAIPEAIINGDTDNRLPNTTNIAFK
HHEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEE
YVEGEAILLLMDGHGICASSGSACTSGSLEPSHVLRAMGVPFTYAHGSIRFSLSRFNTQA
EECCCEEEEEECCCCEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCEEEEEEECCCCCC
DVDYVVDELPRIIARLREISPYRSGEDAQAKPACTC
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]