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The map label for this gene is ddlA [C]

Identifier: 78358082

GI number: 78358082

Start: 3033768

End: 3034817

Strand: Direct

Name: ddlA [C]

Synonym: Dde_3042

Alternate gene names: 78358082

Gene position: 3033768-3034817 (Clockwise)

Preceding gene: 78358081

Following gene: 78358083

Centisome position: 81.33

GC content: 60.86

Gene sequence:

>1050_bases
GTGCTTGTAGGCATTACATACGACCTGAAAGAAGAGTATCTCGCACGGGGCTATTCGGAAGAAGAAGCAGCTGAGTTCGA
CAGCCCCGCCACCATTGAGGGCATAGAAACCGCACTGCAGGCTCTGGGATACGAGACGCAGCGCATCGGGGCGCTTCCCG
CGCTGGCCGCTGCCCTGCTGCAGGGACGCCGCTGGGATATCGTCTTCAACATTGCCGAAGGTTGTCACGGGTTCGGGCGC
GAAAGCCAGGTGCCTGCCCTGCTTGACTACTACAGCGTGCCCTATGTGTTTTCCGACCCCATGACGCTGGGTATCTGCCT
GCATAAAGGCATGACCAAGCATATCGTGCGCGACAACGGCATTCCCACCGCAGATTTTGTGGTGATCAGCGATCCCGCAG
AAGCCGCGGCAGTGAGTCTGCCCTATCCGCTGTTTGTCAAGCCCGTGGCCGAAGGCACCGGCATAGGGGTGGGGGCCGCA
TCAAAGGTGCAGAACCCGCAGGAACTGGCCGCCGCCTGCGCCCGGCTCATCGACCGCCACAGACAGCCCGTGCTGGTGGA
AACGTTTCTGTGCGGCCGTGAACTGACCGTGGGTATCACCGGTACCGGAGCAGGAGCCAGAACACTGGGCGCTCTGGAGG
TGGTATTTGCTCCGGCAGCCGAATCGGATGTGTACGGATATGTGAACAAAGCCGAGTATCTGGAGCGCGTGCAGTATCAT
CTGGCCGATGATGCCGATGCGCAGAAGGCCCGCCATGTGGCACTGGCAGCGTGGCGTGCGCTGGGATGCCGCGACGGCGG
ACGCGTTGACCTGCGCTTTGACGCCCGCGGCGTGCCCAATTTCATTGAAGTGAATCCTCTGGCGGGCCTGAATCCTGTGA
ATTCTGACCTGCCCATCATGTGCTACAAGGCCGGACTGCGCTACGAGCAGCTTATCGGACAGATAATGGAGTCGGCGGTG
GAACGTCTGAAAACGGACACAGGCCAGCGTTTTGTGCCTGCCGTAACGGCGGCGGCATACACCGGACATACGGGGACGGT
GGCGGAATAA

Upstream 100 bases:

>100_bases
ATGAAGGCGGCCTGTACCGCTCGTATGACCCCGCGGTGCCCCTTGCCGCACACAACGTGCACAACGCCGGCCCCGCAGGC
TGCGCAGACGGAGACATGCC

Downstream 100 bases:

>100_bases
TATGCGTGTAGCCGTTGTATACAACGCTGTGCAGCCCGACGGCAGACCCGATCAGGAAGACACCCTGCTTCAGGCGCGTG
CGGTGCACGACGCCCTGATA

Product: D-alanine--D-alanine ligase

Products: ADP; phosphate; D-alanyl-D-alanine

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MLVGITYDLKEEYLARGYSEEEAAEFDSPATIEGIETALQALGYETQRIGALPALAAALLQGRRWDIVFNIAEGCHGFGR
ESQVPALLDYYSVPYVFSDPMTLGICLHKGMTKHIVRDNGIPTADFVVISDPAEAAAVSLPYPLFVKPVAEGTGIGVGAA
SKVQNPQELAAACARLIDRHRQPVLVETFLCGRELTVGITGTGAGARTLGALEVVFAPAAESDVYGYVNKAEYLERVQYH
LADDADAQKARHVALAAWRALGCRDGGRVDLRFDARGVPNFIEVNPLAGLNPVNSDLPIMCYKAGLRYEQLIGQIMESAV
ERLKTDTGQRFVPAVTAAAYTGHTGTVAE

Sequences:

>Translated_349_residues
MLVGITYDLKEEYLARGYSEEEAAEFDSPATIEGIETALQALGYETQRIGALPALAAALLQGRRWDIVFNIAEGCHGFGR
ESQVPALLDYYSVPYVFSDPMTLGICLHKGMTKHIVRDNGIPTADFVVISDPAEAAAVSLPYPLFVKPVAEGTGIGVGAA
SKVQNPQELAAACARLIDRHRQPVLVETFLCGRELTVGITGTGAGARTLGALEVVFAPAAESDVYGYVNKAEYLERVQYH
LADDADAQKARHVALAAWRALGCRDGGRVDLRFDARGVPNFIEVNPLAGLNPVNSDLPIMCYKAGLRYEQLIGQIMESAV
ERLKTDTGQRFVPAVTAAAYTGHTGTVAE
>Mature_349_residues
MLVGITYDLKEEYLARGYSEEEAAEFDSPATIEGIETALQALGYETQRIGALPALAAALLQGRRWDIVFNIAEGCHGFGR
ESQVPALLDYYSVPYVFSDPMTLGICLHKGMTKHIVRDNGIPTADFVVISDPAEAAAVSLPYPLFVKPVAEGTGIGVGAA
SKVQNPQELAAACARLIDRHRQPVLVETFLCGRELTVGITGTGAGARTLGALEVVFAPAAESDVYGYVNKAEYLERVQYH
LADDADAQKARHVALAAWRALGCRDGGRVDLRFDARGVPNFIEVNPLAGLNPVNSDLPIMCYKAGLRYEQLIGQIMESAV
ERLKTDTGQRFVPAVTAAAYTGHTGTVAE

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=279, Percent_Identity=29.7491039426523, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786280, Length=258, Percent_Identity=29.0697674418605, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4

Molecular weight: Translated: 37500; Mature: 37500

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVGITYDLKEEYLARGYSEEEAAEFDSPATIEGIETALQALGYETQRIGALPALAAALL
CEEEEEEHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHH
QGRRWDIVFNIAEGCHGFGRESQVPALLDYYSVPYVFSDPMTLGICLHKGMTKHIVRDNG
CCCCEEEEEEECHHCCCCCCCCCCCHHHHHHCCCEEECCCCEEEHHHHCCCHHHHHHCCC
IPTADFVVISDPAEAAAVSLPYPLFVKPVAEGTGIGVGAASKVQNPQELAAACARLIDRH
CCCCCEEEEECCCCHHEEECCCCEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHC
RQPVLVETFLCGRELTVGITGTGAGARTLGALEVVFAPAAESDVYGYVNKAEYLERVQYH
CCCEEEEHHHCCCEEEEEEECCCCCHHHHHHEEEEEECCCCCCHHHCCCHHHHHHHHHHH
LADDADAQKARHVALAAWRALGCRDGGRVDLRFDARGVPNFIEVNPLAGLNPVNSDLPIM
HCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCEE
CYKAGLRYEQLIGQIMESAVERLKTDTGQRFVPAVTAAAYTGHTGTVAE
EEHHCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLVGITYDLKEEYLARGYSEEEAAEFDSPATIEGIETALQALGYETQRIGALPALAAALL
CEEEEEEHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHH
QGRRWDIVFNIAEGCHGFGRESQVPALLDYYSVPYVFSDPMTLGICLHKGMTKHIVRDNG
CCCCEEEEEEECHHCCCCCCCCCCCHHHHHHCCCEEECCCCEEEHHHHCCCHHHHHHCCC
IPTADFVVISDPAEAAAVSLPYPLFVKPVAEGTGIGVGAASKVQNPQELAAACARLIDRH
CCCCCEEEEECCCCHHEEECCCCEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHC
RQPVLVETFLCGRELTVGITGTGAGARTLGALEVVFAPAAESDVYGYVNKAEYLERVQYH
CCCEEEEHHHCCCEEEEEEECCCCCHHHHHHEEEEEECCCCCCHHHCCCHHHHHHHHHHH
LADDADAQKARHVALAAWRALGCRDGGRVDLRFDARGVPNFIEVNPLAGLNPVNSDLPIM
HCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCEE
CYKAGLRYEQLIGQIMESAVERLKTDTGQRFVPAVTAAAYTGHTGTVAE
EEHHCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-alanine

Specific reaction: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: (3-Amino-2-oxoalkyl) phosphonic acids; alpha-Aminophosphonamidic acid; D-(1-Aminoethyl) phosphonic acid in presence of ATP; D-Cycloserine; Tabtoxinine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]