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The map label for this gene is hipO [H]

Identifier: 78358073

GI number: 78358073

Start: 3020408

End: 3021565

Strand: Direct

Name: hipO [H]

Synonym: Dde_3033

Alternate gene names: 78358073

Gene position: 3020408-3021565 (Clockwise)

Preceding gene: 78358072

Following gene: 78358074

Centisome position: 80.97

GC content: 57.94

Gene sequence:

>1158_bases
ATGCCTGTGAAGCCGGAAATCGAGGCCCGTTTCGAAGAGCTGAAAAGCATACGCCAGTACCTGCACTCCTGTCCCGAAGT
GGGTCTGGAAACAGTGAATACCGCAGCGTTTGTAAAAAAACAGCTGGACGGACTGGGCATCGGATATGAAGACATCGGCG
TCAACTCGCTGCTGGCCAAAGTGGAAGGCACTGCTCCCGGCGTGACAGTGGCCTTCCGGGCCGATATGGACGGACTGGAA
ACCTGCGAAGAAACCGGCCTGCCGTATGCTTCGCAGACCTGTGGCCGCATGCATGCGTGCGGCCACGACGGCCACACCGC
CACGCTGCTGGCTTTTGCCGGGTATCTGGCGCAGCACCGCGATTTCAAAGGAACCGTGCTGCTGTTGTTCCAGTCCGGAG
AAGAAGGCTACGGCGGAGCGCTGGAAGTCATCAAAGACGGTCTGTTTGAAAAATACAGCATCGACTACATGTTCGGCATG
CACAACTGGCCGCCTTACGGCGAAAACCAGATGATAGTGCACAAAGGCACCGCCATGGCCTCCGAAGACCGCTTCGATCT
GGTCATACGCGGCAAAAGCGGACACGCCTCCGTTCCGCACGCCTGCAACGAACCTTTCGCCGCAGTGGCCGACTTCATAA
AAAACGCGCAGAGCATTGTTGCCCGGCGCATTTCGGCACATGACAAAGGCGTCATAAGCATCACACAGGTACACGGCGGC
AGCGCCTACAACATCATTCCCGACGAAGTGACCATACGCGGCAACGTGCGTACCACCGACCCGCGCGTGCAGGACCTCAT
CGAGGAATCGCTGGCACAGCTGGCGCAGGGACTCGAGGTGACCTACGGCGTAAAAGCCGCCTTCACCTACCACCGCAAGC
ACCCGCCGGTCATCAACTCCACGCCGGATATGGCCATAGCCGCCGCGGCGCGTGTGGTGGGACAGGAAAACGTGCTCACT
GAAGAACTGCCCGCCATGGGCAGCGAAGACTACGCCTTTTACATGCAGAAAACCAAAGGGTGTTTTGTATGGATAGGCAA
CGGCACGGATTCCGCGCTGATACATAACAGCAAGTACGACTTCAACGACAAGATCATTCTTCTGGGAGCTTCGCTGTTTG
CGGAGTTGCTGGACGAGGTGCTGTCGGCACAGCCGTAA

Upstream 100 bases:

>100_bases
ATGGCGTATCGTTGTGGTCAGCCTGTTTGTAATGGCAGGAACCTATGTGGGTTCCGCAGCCATCGCCCACGTTGTGCTGA
AATATCTTGGAGAGATATAA

Downstream 100 bases:

>100_bases
CCTGCACAGTCACACTCAGGGGCTGTCCGGCTGCACGACGCCCCGCGGCGCTGAGCGCCGCAGACAGTGCACGCCGCCGC
GGCCCGCGCGGCACGGCGCC

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MPVKPEIEARFEELKSIRQYLHSCPEVGLETVNTAAFVKKQLDGLGIGYEDIGVNSLLAKVEGTAPGVTVAFRADMDGLE
TCEETGLPYASQTCGRMHACGHDGHTATLLAFAGYLAQHRDFKGTVLLLFQSGEEGYGGALEVIKDGLFEKYSIDYMFGM
HNWPPYGENQMIVHKGTAMASEDRFDLVIRGKSGHASVPHACNEPFAAVADFIKNAQSIVARRISAHDKGVISITQVHGG
SAYNIIPDEVTIRGNVRTTDPRVQDLIEESLAQLAQGLEVTYGVKAAFTYHRKHPPVINSTPDMAIAAAARVVGQENVLT
EELPAMGSEDYAFYMQKTKGCFVWIGNGTDSALIHNSKYDFNDKIILLGASLFAELLDEVLSAQP

Sequences:

>Translated_385_residues
MPVKPEIEARFEELKSIRQYLHSCPEVGLETVNTAAFVKKQLDGLGIGYEDIGVNSLLAKVEGTAPGVTVAFRADMDGLE
TCEETGLPYASQTCGRMHACGHDGHTATLLAFAGYLAQHRDFKGTVLLLFQSGEEGYGGALEVIKDGLFEKYSIDYMFGM
HNWPPYGENQMIVHKGTAMASEDRFDLVIRGKSGHASVPHACNEPFAAVADFIKNAQSIVARRISAHDKGVISITQVHGG
SAYNIIPDEVTIRGNVRTTDPRVQDLIEESLAQLAQGLEVTYGVKAAFTYHRKHPPVINSTPDMAIAAAARVVGQENVLT
EELPAMGSEDYAFYMQKTKGCFVWIGNGTDSALIHNSKYDFNDKIILLGASLFAELLDEVLSAQP
>Mature_384_residues
PVKPEIEARFEELKSIRQYLHSCPEVGLETVNTAAFVKKQLDGLGIGYEDIGVNSLLAKVEGTAPGVTVAFRADMDGLET
CEETGLPYASQTCGRMHACGHDGHTATLLAFAGYLAQHRDFKGTVLLLFQSGEEGYGGALEVIKDGLFEKYSIDYMFGMH
NWPPYGENQMIVHKGTAMASEDRFDLVIRGKSGHASVPHACNEPFAAVADFIKNAQSIVARRISAHDKGVISITQVHGGS
AYNIIPDEVTIRGNVRTTDPRVQDLIEESLAQLAQGLEVTYGVKAAFTYHRKHPPVINSTPDMAIAAAARVVGQENVLTE
ELPAMGSEDYAFYMQKTKGCFVWIGNGTDSALIHNSKYDFNDKIILLGASLFAELLDEVLSAQP

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=344, Percent_Identity=27.6162790697674, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41886; Mature: 41755

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKPEIEARFEELKSIRQYLHSCPEVGLETVNTAAFVKKQLDGLGIGYEDIGVNSLLAK
CCCCCHHHHHHHHHHHHHHHHHHCHHHCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHH
VEGTAPGVTVAFRADMDGLETCEETGLPYASQTCGRMHACGHDGHTATLLAFAGYLAQHR
HCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DFKGTVLLLFQSGEEGYGGALEVIKDGLFEKYSIDYMFGMHNWPPYGENQMIVHKGTAMA
CCCCEEEEEEECCCCCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCEEEEECCCCCC
SEDRFDLVIRGKSGHASVPHACNEPFAAVADFIKNAQSIVARRISAHDKGVISITQVHGG
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
SAYNIIPDEVTIRGNVRTTDPRVQDLIEESLAQLAQGLEVTYGVKAAFTYHRKHPPVINS
EEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECEEEEEEECCCCCCCCCC
TPDMAIAAAARVVGQENVLTEELPAMGSEDYAFYMQKTKGCFVWIGNGTDSALIHNSKYD
CCCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCEEEECCCCC
FNDKIILLGASLFAELLDEVLSAQP
CCCCEEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVKPEIEARFEELKSIRQYLHSCPEVGLETVNTAAFVKKQLDGLGIGYEDIGVNSLLAK
CCCCHHHHHHHHHHHHHHHHHHCHHHCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHH
VEGTAPGVTVAFRADMDGLETCEETGLPYASQTCGRMHACGHDGHTATLLAFAGYLAQHR
HCCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DFKGTVLLLFQSGEEGYGGALEVIKDGLFEKYSIDYMFGMHNWPPYGENQMIVHKGTAMA
CCCCEEEEEEECCCCCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCEEEEECCCCCC
SEDRFDLVIRGKSGHASVPHACNEPFAAVADFIKNAQSIVARRISAHDKGVISITQVHGG
CCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
SAYNIIPDEVTIRGNVRTTDPRVQDLIEESLAQLAQGLEVTYGVKAAFTYHRKHPPVINS
EEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECEEEEEEECCCCCCCCCC
TPDMAIAAAARVVGQENVLTEELPAMGSEDYAFYMQKTKGCFVWIGNGTDSALIHNSKYD
CCCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCEEEECCCCC
FNDKIILLGASLFAELLDEVLSAQP
CCCCEEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]