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The map label for this gene is prfA

Identifier: 78358024

GI number: 78358024

Start: 2964600

End: 2965673

Strand: Direct

Name: prfA

Synonym: Dde_2984

Alternate gene names: 78358024

Gene position: 2964600-2965673 (Clockwise)

Preceding gene: 78358023

Following gene: 78358025

Centisome position: 79.47

GC content: 56.98

Gene sequence:

>1074_bases
ATGTTTGCCAAGCTTGAGCACCTGGAACGTCAGTTTGAAGATCTGGAGCAGCAGCTCAGTTCGCCGGAAGTTTTCGGCGA
TCAGGAACGCTACCGCAAACTGACAAAAGCACACTCCGATCTCAAAGAGATTGTGGAGGTTTTCCGCGTCTACCGCCAGC
TGAATGAAGAACTGGCAGGTAACAAGGAAATGCTGCGTGACAGCGACCCCGAAATTCAGGCCATGGCCAGAGAGGAAATA
GACGCCATTGAAAAGCGTCTGCCCGAAATGGAACATGAGCTGAAGCTGCTGCTGCTGCCCAAGGACCCGCTGGATGAAAA
GAACGTGGTTCTGGAAATACGGGCCGGCACCGGCGGTGAAGAAGCAGCCCTGTTCGCTGCCGACCTTTTCCGCATGTACC
TGCGCTATGCCGAAGACATGGGCTGGCGCGTGGAAGTGCTGAGCAGCTCGGAAACCGACTCCGGCGGCTTCAAGGAAATT
ATCGGGCTTATTTCCGGCGATAAAGTTTACAGCCGCATGAAATTTGAATCCGGAACCCACAGAGTGCAGCGCGTGCCCGC
CACCGAAACGCAGGGGCGTATCCATACGTCCGCAGCCACGGTTGCCGTTATGCCCGAAGCCGACGAAGTGGAACTGGACA
TGAAGCCCGAAGATCTGCGTTTTGACGTCTACCGTTCTTCCGGCCCCGGCGGCCAGTCTGTGAACACCACCGACTCCGCC
GTGCGCGTGACGCATATCCCCACGGGCATCACCGTGGCGTGTCAGGATGAAAAATCGCAGCACAAAAACAAGGCGAAAGG
GCTTAAGATTCTTGCCTCCCGCCTGCTGCAGGCTCAGGAAGACAAACGGCACGAAGAACTGGCAGAACAGCGCCGCAGCC
TTGTGGGCACGGGAGACAGATCGGGCCGCATACGCACCTACAATTTCCCGCAGGGGCGCATAACTGACCACCGTATCAAC
CTCACCCTGTACAAGCTGGACGCGTTCATGGAAGGTCAGATAGGCGACATGCTCGATGCCCTGATCACCCACGCTCAGAC
CGAAGCTCTCAAGGCGCAGGCCAACGTGCATTAA

Upstream 100 bases:

>100_bases
GCCAGATAGAAGTGGCCATAGTGGCGCTGCGCGAAGCACTGGGCCCGGACGCCGGTGAAGAAATCACGACGCCAGAATAC
ACCTATTCGGAGTAATTTCG

Downstream 100 bases:

>100_bases
TCATGTGCACCAGCGTTACCCCCGCTTCCCGCACCATCCGCGACACTCTTACCGCCTTCTCGGCCCTGCTGGCCGGGAAG
GCGGTTGATTCTCCGCGGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFAKLEHLERQFEDLEQQLSSPEVFGDQERYRKLTKAHSDLKEIVEVFRVYRQLNEELAGNKEMLRDSDPEIQAMAREEI
DAIEKRLPEMEHELKLLLLPKDPLDEKNVVLEIRAGTGGEEAALFAADLFRMYLRYAEDMGWRVEVLSSSETDSGGFKEI
IGLISGDKVYSRMKFESGTHRVQRVPATETQGRIHTSAATVAVMPEADEVELDMKPEDLRFDVYRSSGPGGQSVNTTDSA
VRVTHIPTGITVACQDEKSQHKNKAKGLKILASRLLQAQEDKRHEELAEQRRSLVGTGDRSGRIRTYNFPQGRITDHRIN
LTLYKLDAFMEGQIGDMLDALITHAQTEALKAQANVH

Sequences:

>Translated_357_residues
MFAKLEHLERQFEDLEQQLSSPEVFGDQERYRKLTKAHSDLKEIVEVFRVYRQLNEELAGNKEMLRDSDPEIQAMAREEI
DAIEKRLPEMEHELKLLLLPKDPLDEKNVVLEIRAGTGGEEAALFAADLFRMYLRYAEDMGWRVEVLSSSETDSGGFKEI
IGLISGDKVYSRMKFESGTHRVQRVPATETQGRIHTSAATVAVMPEADEVELDMKPEDLRFDVYRSSGPGGQSVNTTDSA
VRVTHIPTGITVACQDEKSQHKNKAKGLKILASRLLQAQEDKRHEELAEQRRSLVGTGDRSGRIRTYNFPQGRITDHRIN
LTLYKLDAFMEGQIGDMLDALITHAQTEALKAQANVH
>Mature_357_residues
MFAKLEHLERQFEDLEQQLSSPEVFGDQERYRKLTKAHSDLKEIVEVFRVYRQLNEELAGNKEMLRDSDPEIQAMAREEI
DAIEKRLPEMEHELKLLLLPKDPLDEKNVVLEIRAGTGGEEAALFAADLFRMYLRYAEDMGWRVEVLSSSETDSGGFKEI
IGLISGDKVYSRMKFESGTHRVQRVPATETQGRIHTSAATVAVMPEADEVELDMKPEDLRFDVYRSSGPGGQSVNTTDSA
VRVTHIPTGITVACQDEKSQHKNKAKGLKILASRLLQAQEDKRHEELAEQRRSLVGTGDRSGRIRTYNFPQGRITDHRIN
LTLYKLDAFMEGQIGDMLDALITHAQTEALKAQANVH

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=320, Percent_Identity=40.9375, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI34577120, Length=349, Percent_Identity=39.2550143266476, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI166795305, Length=216, Percent_Identity=42.1296296296296, Blast_Score=192, Evalue=5e-49,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=55.8073654390935, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI2367172, Length=344, Percent_Identity=36.9186046511628, Blast_Score=211, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=288, Percent_Identity=37.5, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6321295, Length=358, Percent_Identity=40.5027932960894, Blast_Score=244, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=347, Percent_Identity=38.0403458213257, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_DESDG (Q30X18)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389473.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q30X18
- SMR:   Q30X18
- STRING:   Q30X18
- GeneID:   3757989
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2984
- NMPDR:   fig|207559.3.peg.3039
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   GEAGGYK
- ProtClustDB:   PRK00591
- BioCyc:   DDES207559:DDE_2984-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40449; Mature: 40449

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAKLEHLERQFEDLEQQLSSPEVFGDQERYRKLTKAHSDLKEIVEVFRVYRQLNEELAG
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKEMLRDSDPEIQAMAREEIDAIEKRLPEMEHELKLLLLPKDPLDEKNVVLEIRAGTGGE
CHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAADLFRMYLRYAEDMGWRVEVLSSSETDSGGFKEIIGLISGDKVYSRMKFESGTH
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCH
RVQRVPATETQGRIHTSAATVAVMPEADEVELDMKPEDLRFDVYRSSGPGGQSVNTTDSA
HHHCCCCCCCCCEEEECCEEEEECCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCE
VRVTHIPTGITVACQDEKSQHKNKAKGLKILASRLLQAQEDKRHEELAEQRRSLVGTGDR
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGRIRTYNFPQGRITDHRINLTLYKLDAFMEGQIGDMLDALITHAQTEALKAQANVH
CCCEEEEECCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFAKLEHLERQFEDLEQQLSSPEVFGDQERYRKLTKAHSDLKEIVEVFRVYRQLNEELAG
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NKEMLRDSDPEIQAMAREEIDAIEKRLPEMEHELKLLLLPKDPLDEKNVVLEIRAGTGGE
CHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCCEEEEEECCCCCC
EAALFAADLFRMYLRYAEDMGWRVEVLSSSETDSGGFKEIIGLISGDKVYSRMKFESGTH
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCH
RVQRVPATETQGRIHTSAATVAVMPEADEVELDMKPEDLRFDVYRSSGPGGQSVNTTDSA
HHHCCCCCCCCCEEEECCEEEEECCCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCCCE
VRVTHIPTGITVACQDEKSQHKNKAKGLKILASRLLQAQEDKRHEELAEQRRSLVGTGDR
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGRIRTYNFPQGRITDHRINLTLYKLDAFMEGQIGDMLDALITHAQTEALKAQANVH
CCCEEEEECCCCCCCCEEEEEEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA