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The map label for this gene is hemK [H]

Identifier: 78358025

GI number: 78358025

Start: 2965676

End: 2966569

Strand: Direct

Name: hemK [H]

Synonym: Dde_2985

Alternate gene names: 78358025

Gene position: 2965676-2966569 (Clockwise)

Preceding gene: 78358024

Following gene: 78358026

Centisome position: 79.5

GC content: 62.75

Gene sequence:

>894_bases
ATGTGCACCAGCGTTACCCCCGCTTCCCGCACCATCCGCGACACTCTTACCGCCTTCTCGGCCCTGCTGGCCGGGAAGGC
GGTTGATTCTCCGCGGCTTTCCGCCGAGCTGCTGCTTGCGCACGTGCTGCGCACCGACAGGCTGCAGCTGCTTGTCCGGC
GCGGGCACATGCTGACAGAAAAAGAATACGCACAGGCGGAAAAACTCATCCTGCGCAGAGCGGAGGGAGAACCTGTGGCC
TACCTGACGGGCAGCCGCGAATTCTACGGCAGGGAATTTGCTGTCTCGACGGACACGCTCATCCCCCGTCCGGACACAGA
GCTGCTGATAGACACGCTGAAAAAGGAATACCCGCCGCACGCCGCACTGCGGTTTGCCGATCTGGGCACCGGCAGCGGAT
GCATTGCCGTCAGCGTTGCAGCCGAGATGCCCTCGGCGCACGGCACTGCTGTGGATATCAGCTCCGGAGCGCTGCACACC
GCCAGGGAAAACGCGGCACGGCACCGCGTTGCCGACAGGGTAGCCTTTGTGCAGGCAGACTTCACCTCACCTCTTTTCCG
ACCCGCATCATTCGACGTGGTGCTTTCCAATCCACCCTATGTCAGCGCCACGGAATACGAGACGCTCAGCCCCGAAGTGC
GCTGCCACGAACCGCAAAGGGCGCTGGTACCTGACACGCCGGCTTCCACCGGGCTGGAACACGCGGCGGCCCTGCTGCCG
CTGGCTTTCGGCTGGCTGAAACCGGGAGGACTCTTCCTTATGGAATTCGGCTGGAAACAAGGCCCCGACATCATGGCCAT
GGTCAAAGCGCAACATGGACAGTGGACCGTTGCGGTCATCCTGCAGGACCTTGCGGGCAGAGACAGAGCCTTGTACGCAC
GTCGCTGTGATTGA

Upstream 100 bases:

>100_bases
TGGACGCGTTCATGGAAGGTCAGATAGGCGACATGCTCGATGCCCTGATCACCCACGCTCAGACCGAAGCTCTCAAGGCG
CAGGCCAACGTGCATTAATC

Downstream 100 bases:

>100_bases
TGTAAAAAAACCACATGGTGTAAAAAACACACAGTTGCGGCACAGCCTGCCTGAAAAAACATCTCATAAAATCAGCGGAC
TACAACCAATACCGCAACGG

Product: HemK family modification methylase

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MCTSVTPASRTIRDTLTAFSALLAGKAVDSPRLSAELLLAHVLRTDRLQLLVRRGHMLTEKEYAQAEKLILRRAEGEPVA
YLTGSREFYGREFAVSTDTLIPRPDTELLIDTLKKEYPPHAALRFADLGTGSGCIAVSVAAEMPSAHGTAVDISSGALHT
ARENAARHRVADRVAFVQADFTSPLFRPASFDVVLSNPPYVSATEYETLSPEVRCHEPQRALVPDTPASTGLEHAAALLP
LAFGWLKPGGLFLMEFGWKQGPDIMAMVKAQHGQWTVAVILQDLAGRDRALYARRCD

Sequences:

>Translated_297_residues
MCTSVTPASRTIRDTLTAFSALLAGKAVDSPRLSAELLLAHVLRTDRLQLLVRRGHMLTEKEYAQAEKLILRRAEGEPVA
YLTGSREFYGREFAVSTDTLIPRPDTELLIDTLKKEYPPHAALRFADLGTGSGCIAVSVAAEMPSAHGTAVDISSGALHT
ARENAARHRVADRVAFVQADFTSPLFRPASFDVVLSNPPYVSATEYETLSPEVRCHEPQRALVPDTPASTGLEHAAALLP
LAFGWLKPGGLFLMEFGWKQGPDIMAMVKAQHGQWTVAVILQDLAGRDRALYARRCD
>Mature_297_residues
MCTSVTPASRTIRDTLTAFSALLAGKAVDSPRLSAELLLAHVLRTDRLQLLVRRGHMLTEKEYAQAEKLILRRAEGEPVA
YLTGSREFYGREFAVSTDTLIPRPDTELLIDTLKKEYPPHAALRFADLGTGSGCIAVSVAAEMPSAHGTAVDISSGALHT
ARENAARHRVADRVAFVQADFTSPLFRPASFDVVLSNPPYVSATEYETLSPEVRCHEPQRALVPDTPASTGLEHAAALLP
LAFGWLKPGGLFLMEFGWKQGPDIMAMVKAQHGQWTVAVILQDLAGRDRALYARRCD

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=297, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=9e-21,
Organism=Escherichia coli, GI1787463, Length=236, Percent_Identity=37.7118644067797, Blast_Score=138, Evalue=5e-34,
Organism=Escherichia coli, GI87082085, Length=272, Percent_Identity=31.9852941176471, Blast_Score=103, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24582226, Length=264, Percent_Identity=25, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32403; Mature: 32403

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00626 RCC1_2 ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSVTPASRTIRDTLTAFSALLAGKAVDSPRLSAELLLAHVLRTDRLQLLVRRGHMLTE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KEYAQAEKLILRRAEGEPVAYLTGSREFYGREFAVSTDTLIPRPDTELLIDTLKKEYPPH
HHHHHHHHHHHHHCCCCCEEEEECCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCC
AALRFADLGTGSGCIAVSVAAEMPSAHGTAVDISSGALHTARENAARHRVADRVAFVQAD
HHEEEEECCCCCCEEEEEEEHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEEC
FTSPLFRPASFDVVLSNPPYVSATEYETLSPEVRCHEPQRALVPDTPASTGLEHAAALLP
CCCCCCCCCCEEEEECCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHH
LAFGWLKPGGLFLMEFGWKQGPDIMAMVKAQHGQWTVAVILQDLAGRDRALYARRCD
HHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCEEHHHHHHHHCCCCHHHHEECCC
>Mature Secondary Structure
MCTSVTPASRTIRDTLTAFSALLAGKAVDSPRLSAELLLAHVLRTDRLQLLVRRGHMLTE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KEYAQAEKLILRRAEGEPVAYLTGSREFYGREFAVSTDTLIPRPDTELLIDTLKKEYPPH
HHHHHHHHHHHHHCCCCCEEEEECCHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCC
AALRFADLGTGSGCIAVSVAAEMPSAHGTAVDISSGALHTARENAARHRVADRVAFVQAD
HHEEEEECCCCCCEEEEEEEHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHEEEEC
FTSPLFRPASFDVVLSNPPYVSATEYETLSPEVRCHEPQRALVPDTPASTGLEHAAALLP
CCCCCCCCCCEEEEECCCCEECCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHH
LAFGWLKPGGLFLMEFGWKQGPDIMAMVKAQHGQWTVAVILQDLAGRDRALYARRCD
HHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCEEHHHHHHHHCCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]