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The map label for this gene is phsB [H]

Identifier: 78357984

GI number: 78357984

Start: 2923426

End: 2923893

Strand: Reverse

Name: phsB [H]

Synonym: Dde_2944

Alternate gene names: 78357984

Gene position: 2923893-2923426 (Counterclockwise)

Preceding gene: 78357985

Following gene: 78357983

Centisome position: 78.38

GC content: 60.9

Gene sequence:

>468_bases
ATGAAGATTTTACGCGCTTCACGCATGGAGCGCTGCATAGGATGTCATTCCTGTTCACTGGCCTGTGCGCGTCTGGTGCA
CAAAAGGCTTTCATGGAATACGGCAGGAATCAGAATTTCCTCGGCCGGCGGTCTTTCCACGGGGTTCGAGGCGCGCACAT
GCGTGGCGTGCAATCCCGCCCCGTGTGCCACCGCCTGCCCCACCGGAGCATTTTCACAGCGCAAGGGCGGCGGTGTCAAA
GTGGACAGGTCGCTGTGCATCCGCTGTGGCAATTGTGCGGAGGCCTGTCCTGTTGAGGCCATTTATATCGATCCTGAAGA
GTCGTTTCCCTACGTCTGCATTCACTGCGGGCGCTGTGTGCCTTTCTGCCCGCATGCCTGTCTTGAGCTGGCAGATGCCG
ATGAAGGCCGGCAGGAATACGGCGGCGGGCAGGGCACCGGGACCGGTCGCGACCACGGAGGCCAGTGA

Upstream 100 bases:

>100_bases
CTGCGGCGCGCGCTGCAGGCGGTGTATCTTTTTACTGCTGCGGGTTGCCCCTGCAGCAGCATTGCAGACCGCGCTTCAGC
GGCGGTACGGAGTTACGCGT

Downstream 100 bases:

>100_bases
TGCGTATTGATTTCACATCGTCAAGAGTTCTGCATGTGGACATGGAGAGCGGGGCATCGCGGGTTATCAGTTTCGGCACC
CGCAGCGCGCATCTGGGCGG

Product: iron-sulfur cluster-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MKILRASRMERCIGCHSCSLACARLVHKRLSWNTAGIRISSAGGLSTGFEARTCVACNPAPCATACPTGAFSQRKGGGVK
VDRSLCIRCGNCAEACPVEAIYIDPEESFPYVCIHCGRCVPFCPHACLELADADEGRQEYGGGQGTGTGRDHGGQ

Sequences:

>Translated_155_residues
MKILRASRMERCIGCHSCSLACARLVHKRLSWNTAGIRISSAGGLSTGFEARTCVACNPAPCATACPTGAFSQRKGGGVK
VDRSLCIRCGNCAEACPVEAIYIDPEESFPYVCIHCGRCVPFCPHACLELADADEGRQEYGGGQGTGTGRDHGGQ
>Mature_155_residues
MKILRASRMERCIGCHSCSLACARLVHKRLSWNTAGIRISSAGGLSTGFEARTCVACNPAPCATACPTGAFSQRKGGGVK
VDRSLCIRCGNCAEACPVEAIYIDPEESFPYVCIHCGRCVPFCPHACLELADADEGRQEYGGGQGTGTGRDHGGQ

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787963, Length=144, Percent_Identity=26.3888888888889, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI87082180, Length=150, Percent_Identity=32, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI226510944, Length=165, Percent_Identity=30.3030303030303, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 16329; Mature: 16329

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
12.3 %Cys+Met (Translated Protein)
11.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
12.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILRASRMERCIGCHSCSLACARLVHKRLSWNTAGIRISSAGGLSTGFEARTCVACNPA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCC
PCATACPTGAFSQRKGGGVKVDRSLCIRCGNCAEACPVEAIYIDPEESFPYVCIHCGRCV
CCCCCCCCCCCCCCCCCCEEECHHHEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCC
PFCPHACLELADADEGRQEYGGGQGTGTGRDHGGQ
CCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKILRASRMERCIGCHSCSLACARLVHKRLSWNTAGIRISSAGGLSTGFEARTCVACNPA
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCEEEECCCC
PCATACPTGAFSQRKGGGVKVDRSLCIRCGNCAEACPVEAIYIDPEESFPYVCIHCGRCV
CCCCCCCCCCCCCCCCCCEEECHHHEEEECCCCCCCCCEEEEECCCCCCCEEEEECCCCC
PFCPHACLELADADEGRQEYGGGQGTGTGRDHGGQ
CCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur; Iron-Sulfur; Iron-Sulfur; Iron-Sulfur; Iron-Sulfur; Iron-Sulfur; Iron-Sulfur; Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]