Click here to switch to the map view.

The map label for this gene is nhaA

Identifier: 78357813

GI number: 78357813

Start: 2773064

End: 2774416

Strand: Reverse

Name: nhaA

Synonym: Dde_2771

Alternate gene names: 78357813

Gene position: 2774416-2773064 (Counterclockwise)

Preceding gene: 78357815

Following gene: 78357812

Centisome position: 74.38

GC content: 58.61

Gene sequence:

>1353_bases
ATGCAACACCACGACATACCCGGGGCCGCCCCCCAGCCCATCGACAAACTGCTGGCGCCTTTCAGGCGTTTCTTTGCCAT
CGAGGCATCCAGCGGCATTCTGCTGATGGCCGCCACCGTGCTGGCGCTGGTATGGGCAAACTCAGGCTTTTCTGCATCGT
ACCATGCGCTGTGGAGCAATAAAGTAACCGTGGGCTTCGGCGACTACGCCTTGTCCAAAGCTCTTATCCTGTGGATCAAC
GACGGTCTGATGGCCATATTTTTCTTTGTGGTCGGACTGGAGATAAAGCGCGAAATTCTGGCGGGTGAACTGGCATCGCC
CAGGCAGGCAGCGCTGCCTATTGCCGCGGCCATAGGCGGCATGCTGGTTCCCGCAGGCATTTATCTGGCGCTTAACGGCG
GTACCGCTGCCGCCAGCGGCTGGGGCGTGCCCATGGCCACCGACATTGCTTTTGCCCTTGGTATTCTTTCGCTTCTGGGT
GACCGTGTACCGCTTAGCCTCAAAGTGTTTCTTACGGCAGTGGCCATTGTGGATGATCTGGGTGCCATTCTGGTCATCGC
ATTTTTTTATACTGCAAACCTGTCGTTCAGCTTTCTCATGCTGGGGTTTGCGGCGTTTGCGGTCATGCTGCTGCTCAACT
GGCTGGGCGTGCGCAGGGTAACCCCGTACCTGCTTGTGGGGCTGGTGCTGTGGTTTGCCCTGCTGAAATCCGGCGTGCAT
GCCACCATAGCCGGCGTGCTGGGTGCCATGGCCATACCGGCCCGCTCCGCACTGGCACCGGCTGTTCTGCGCGGTACTGC
CCGTCAGGCGCTTTCCGTGTTTGAAAACGCCCTTCAGGGCGATACGCCTGTGCTTGCCAGTCAGGATAAATCGCATGCCA
TACATCATGTGGAAATGCTGACCGAAAAGGCGGGCACACCTTTGCAGCGGCTTGAGCATGCCCTGCATCCGTGGGTGGCA
TGGTTCATCATGCCTGTCTTTGCTCTTGCCAACGCAGGTGTCACCGTCAGCGCCGAAATGGTGAGCATGCTGTTTGAACC
GCTTTCCCTCGGCATATTCTTCGGCCTGCTGCTGGGCAAGCAGGGCGGCGTCACGCTGGCGGTGTGGCTGCTGGTAAAGA
CCGGTATCGCCAAGCTGCCGAAGGGTGTCGGTCTGGGGATGTACTACGGTATCGGCTGGCTTGCCGGTATCGGCTTCACC
ATGGCCATTTTCATCGCCACACTGGCGTTTGAAGACCCTGCACATATTGAGGCCGCCAAGATGAGTATTCTTTGTGCCTC
GTTTGTGGCCGGTTTCGGCGGGTATATGCTGATGCGTGTGCTGCTGCGCGGTCGCGAGCCTGAAAAGGCTTAG

Upstream 100 bases:

>100_bases
ATCTACAACGGTGCTGTTTCGTTCTGTAGTGATGCTTGGGGGATAGAAAGGGCTATGCCAGTTTCCGGTCATATCAAAGC
AACCAACGGGAGGTTTCTAC

Downstream 100 bases:

>100_bases
TATCCTCCTGGTTATGAAGGGCGCGGCAACGCGCCCTTTTTTTGTGTCTGCCGCCGGTCAGACCGGTGCGGAAGACCGGT
GCGGAAGACCGGTGCCCCGC

Product: Na+/H+ antiporter NhaA

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MQHHDIPGAAPQPIDKLLAPFRRFFAIEASSGILLMAATVLALVWANSGFSASYHALWSNKVTVGFGDYALSKALILWIN
DGLMAIFFFVVGLEIKREILAGELASPRQAALPIAAAIGGMLVPAGIYLALNGGTAAASGWGVPMATDIAFALGILSLLG
DRVPLSLKVFLTAVAIVDDLGAILVIAFFYTANLSFSFLMLGFAAFAVMLLLNWLGVRRVTPYLLVGLVLWFALLKSGVH
ATIAGVLGAMAIPARSALAPAVLRGTARQALSVFENALQGDTPVLASQDKSHAIHHVEMLTEKAGTPLQRLEHALHPWVA
WFIMPVFALANAGVTVSAEMVSMLFEPLSLGIFFGLLLGKQGGVTLAVWLLVKTGIAKLPKGVGLGMYYGIGWLAGIGFT
MAIFIATLAFEDPAHIEAAKMSILCASFVAGFGGYMLMRVLLRGREPEKA

Sequences:

>Translated_450_residues
MQHHDIPGAAPQPIDKLLAPFRRFFAIEASSGILLMAATVLALVWANSGFSASYHALWSNKVTVGFGDYALSKALILWIN
DGLMAIFFFVVGLEIKREILAGELASPRQAALPIAAAIGGMLVPAGIYLALNGGTAAASGWGVPMATDIAFALGILSLLG
DRVPLSLKVFLTAVAIVDDLGAILVIAFFYTANLSFSFLMLGFAAFAVMLLLNWLGVRRVTPYLLVGLVLWFALLKSGVH
ATIAGVLGAMAIPARSALAPAVLRGTARQALSVFENALQGDTPVLASQDKSHAIHHVEMLTEKAGTPLQRLEHALHPWVA
WFIMPVFALANAGVTVSAEMVSMLFEPLSLGIFFGLLLGKQGGVTLAVWLLVKTGIAKLPKGVGLGMYYGIGWLAGIGFT
MAIFIATLAFEDPAHIEAAKMSILCASFVAGFGGYMLMRVLLRGREPEKA
>Mature_450_residues
MQHHDIPGAAPQPIDKLLAPFRRFFAIEASSGILLMAATVLALVWANSGFSASYHALWSNKVTVGFGDYALSKALILWIN
DGLMAIFFFVVGLEIKREILAGELASPRQAALPIAAAIGGMLVPAGIYLALNGGTAAASGWGVPMATDIAFALGILSLLG
DRVPLSLKVFLTAVAIVDDLGAILVIAFFYTANLSFSFLMLGFAAFAVMLLLNWLGVRRVTPYLLVGLVLWFALLKSGVH
ATIAGVLGAMAIPARSALAPAVLRGTARQALSVFENALQGDTPVLASQDKSHAIHHVEMLTEKAGTPLQRLEHALHPWVA
WFIMPVFALANAGVTVSAEMVSMLFEPLSLGIFFGLLLGKQGGVTLAVWLLVKTGIAKLPKGVGLGMYYGIGWLAGIGFT
MAIFIATLAFEDPAHIEAAKMSILCASFVAGFGGYMLMRVLLRGREPEKA

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=428, Percent_Identity=46.7289719626168, Blast_Score=311, Evalue=4e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_DESDG (Q30XM9)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389262.1
- ProteinModelPortal:   Q30XM9
- SMR:   Q30XM9
- STRING:   Q30XM9
- GeneID:   3757794
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2771
- NMPDR:   fig|207559.3.peg.3287
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   YFALRDL
- ProtClustDB:   CLSK942510
- BioCyc:   DDES207559:DDE_2771-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 47657; Mature: 47657

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x13c1023c)-; HASH(0x13d8a288)-; HASH(0x13d90e58)-; HASH(0x1390af18)-; HASH(0x13a5de84)-; HASH(0x13bc020c)-; HASH(0x13b5c3c4)-; HASH(0x13367e14)-; HASH(0x13d36d30)-; HASH(0x13d8a2e8)-; HASH(0x13ab6c7c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHHDIPGAAPQPIDKLLAPFRRFFAIEASSGILLMAATVLALVWANSGFSASYHALWSN
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCC
KVTVGFGDYALSKALILWINDGLMAIFFFVVGLEIKREILAGELASPRQAALPIAAAIGG
CEEEEECHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
MLVPAGIYLALNGGTAAASGWGVPMATDIAFALGILSLLGDRVPLSLKVFLTAVAIVDDL
HHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GAILVIAFFYTANLSFSFLMLGFAAFAVMLLLNWLGVRRVTPYLLVGLVLWFALLKSGVH
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ATIAGVLGAMAIPARSALAPAVLRGTARQALSVFENALQGDTPVLASQDKSHAIHHVEML
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TEKAGTPLQRLEHALHPWVAWFIMPVFALANAGVTVSAEMVSMLFEPLSLGIFFGLLLGK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCC
QGGVTLAVWLLVKTGIAKLPKGVGLGMYYGIGWLAGIGFTMAIFIATLAFEDPAHIEAAK
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MSILCASFVAGFGGYMLMRVLLRGREPEKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure
MQHHDIPGAAPQPIDKLLAPFRRFFAIEASSGILLMAATVLALVWANSGFSASYHALWSN
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCC
KVTVGFGDYALSKALILWINDGLMAIFFFVVGLEIKREILAGELASPRQAALPIAAAIGG
CEEEEECHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
MLVPAGIYLALNGGTAAASGWGVPMATDIAFALGILSLLGDRVPLSLKVFLTAVAIVDDL
HHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GAILVIAFFYTANLSFSFLMLGFAAFAVMLLLNWLGVRRVTPYLLVGLVLWFALLKSGVH
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
ATIAGVLGAMAIPARSALAPAVLRGTARQALSVFENALQGDTPVLASQDKSHAIHHVEML
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
TEKAGTPLQRLEHALHPWVAWFIMPVFALANAGVTVSAEMVSMLFEPLSLGIFFGLLLGK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHCC
QGGVTLAVWLLVKTGIAKLPKGVGLGMYYGIGWLAGIGFTMAIFIATLAFEDPAHIEAAK
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MSILCASFVAGFGGYMLMRVLLRGREPEKA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA