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The map label for this gene is lipB

Identifier: 78357755

GI number: 78357755

Start: 2715204

End: 2715860

Strand: Direct

Name: lipB

Synonym: Dde_2713

Alternate gene names: 78357755

Gene position: 2715204-2715860 (Clockwise)

Preceding gene: 78357754

Following gene: 78357756

Centisome position: 72.79

GC content: 60.73

Gene sequence:

>657_bases
ATGCAGATTATCGATCTGGGGTGCATACCCTACGCCGAAGCCGTGGAAATACAGACCGGACGGGTGCAGGAGGTTGCCGC
AGGAGCGGAAAACACCCTGTACCTGCTGGAACACCCGAAGGTCATAACCTTCGGGCGCAACGGCGGCCGCGAAAACCTGC
ACGTGGACGACAGCACTCTGGCTTCCATGGGTATAGAGGTGGCGCAGACCACCCGCGGCGGCAACATAACCTGCCATTTT
CCCGGCCAGCTTGTCGCCTACCCCGTCTTCCGCATTGCGGGGCGCCCCGGTGGCATGCGGGCCCTGTTTTACAGTCTTGA
GGAAGTGGTCATCAGGACGCTGGCCCGTTTCGGGCTGCAGGCCTTCCGCAGTGAAGGCCGCCCCGGTGTCTGGATAGAAA
ACCGCAAAATATGCTCCATCGGCATTGCCATGCGCCGGTGGACCTCGTACCACGGGCTGGCACTCAACGTGTGCCGCGAT
GTAAGCCTGTTTGACATGATAACGCTGTGCGGCCTGACAGATGCCCGCGCCACGTCACTGCAGGCCGAATGTAACGATGA
CAGCATCACCCTGCAGGAGGTGAAAGATGTCTGCGCAGGAGAATTCCGCGCCGTTTTTGCGCATCCCCCCGTGGCTGCGC
ACCAGAATTCCGTGTAA

Upstream 100 bases:

>100_bases
GCAGGCAGCAGCCGCAAGCCGAAAAAAACAACAAAACCGGCACCGTCATCGGGCGGCAGACGAGGACGGTAATCCGGAAC
GCAGTGGAGACGCGCAGGCA

Downstream 100 bases:

>100_bases
CCGCACCTATACGGCTACCAGAGAGCTCATAGGCGATCTTAATCTGCATACGGTCTGCCAGTCGGCCAAGTGTCCGAACA
TGTTCGAGTGCTTTTCCAGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MQIIDLGCIPYAEAVEIQTGRVQEVAAGAENTLYLLEHPKVITFGRNGGRENLHVDDSTLASMGIEVAQTTRGGNITCHF
PGQLVAYPVFRIAGRPGGMRALFYSLEEVVIRTLARFGLQAFRSEGRPGVWIENRKICSIGIAMRRWTSYHGLALNVCRD
VSLFDMITLCGLTDARATSLQAECNDDSITLQEVKDVCAGEFRAVFAHPPVAAHQNSV

Sequences:

>Translated_218_residues
MQIIDLGCIPYAEAVEIQTGRVQEVAAGAENTLYLLEHPKVITFGRNGGRENLHVDDSTLASMGIEVAQTTRGGNITCHF
PGQLVAYPVFRIAGRPGGMRALFYSLEEVVIRTLARFGLQAFRSEGRPGVWIENRKICSIGIAMRRWTSYHGLALNVCRD
VSLFDMITLCGLTDARATSLQAECNDDSITLQEVKDVCAGEFRAVFAHPPVAAHQNSV
>Mature_218_residues
MQIIDLGCIPYAEAVEIQTGRVQEVAAGAENTLYLLEHPKVITFGRNGGRENLHVDDSTLASMGIEVAQTTRGGNITCHF
PGQLVAYPVFRIAGRPGGMRALFYSLEEVVIRTLARFGLQAFRSEGRPGVWIENRKICSIGIAMRRWTSYHGLALNVCRD
VSLFDMITLCGLTDARATSLQAECNDDSITLQEVKDVCAGEFRAVFAHPPVAAHQNSV

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=218, Percent_Identity=34.8623853211009, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI87081767, Length=172, Percent_Identity=36.046511627907, Blast_Score=107, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323268, Length=142, Percent_Identity=31.6901408450704, Blast_Score=75, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355483, Length=209, Percent_Identity=30.1435406698565, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_DESDG (Q30XT7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389204.1
- ProteinModelPortal:   Q30XT7
- SMR:   Q30XT7
- STRING:   Q30XT7
- GeneID:   3757736
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2713
- NMPDR:   fig|207559.3.peg.3066
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   CLSK704928
- BioCyc:   DDES207559:DDE_2713-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23867; Mature: 23867

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIIDLGCIPYAEAVEIQTGRVQEVAAGAENTLYLLEHPKVITFGRNGGRENLHVDDSTL
CEEEECCCCCCCCEEEECCCCHHHHHCCCCCEEEEEECCEEEEECCCCCCCCEEECHHHH
ASMGIEVAQTTRGGNITCHFPGQLVAYPVFRIAGRPGGMRALFYSLEEVVIRTLARFGLQ
HHHCCEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AFRSEGRPGVWIENRKICSIGIAMRRWTSYHGLALNVCRDVSLFDMITLCGLTDARATSL
HHHCCCCCCEEECCCEEEEHHHHHHHHCCCCCEEEEHHCCCHHHHHHHHHCCCCCHHHEE
QAECNDDSITLQEVKDVCAGEFRAVFAHPPVAAHQNSV
EEECCCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MQIIDLGCIPYAEAVEIQTGRVQEVAAGAENTLYLLEHPKVITFGRNGGRENLHVDDSTL
CEEEECCCCCCCCEEEECCCCHHHHHCCCCCEEEEEECCEEEEECCCCCCCCEEECHHHH
ASMGIEVAQTTRGGNITCHFPGQLVAYPVFRIAGRPGGMRALFYSLEEVVIRTLARFGLQ
HHHCCEEEEECCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AFRSEGRPGVWIENRKICSIGIAMRRWTSYHGLALNVCRDVSLFDMITLCGLTDARATSL
HHHCCCCCCEEECCCEEEEHHHHHHHHCCCCCEEEEHHCCCHHHHHHHHHCCCCCHHHEE
QAECNDDSITLQEVKDVCAGEFRAVFAHPPVAAHQNSV
EEECCCCCEEHHHHHHHHCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA