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The map label for this gene is lipA

Identifier: 78357756

GI number: 78357756

Start: 2715790

End: 2716665

Strand: Direct

Name: lipA

Synonym: Dde_2714

Alternate gene names: 78357756

Gene position: 2715790-2716665 (Clockwise)

Preceding gene: 78357755

Following gene: 78357771

Centisome position: 72.8

GC content: 60.96

Gene sequence:

>876_bases
ATGTCTGCGCAGGAGAATTCCGCGCCGTTTTTGCGCATCCCCCCGTGGCTGCGCACCAGAATTCCGTGTAACCGCACCTA
TACGGCTACCAGAGAGCTCATAGGCGATCTTAATCTGCATACGGTCTGCCAGTCGGCCAAGTGTCCGAACATGTTCGAGT
GCTTTTCCAGCCGCACGGCCACATTTCTCATTCTGGGCGGTACCTGCACGCGCAACTGCGCCTTCTGCAATATCGAACCG
GGTGACGTACTGCCCCCCGACGCCGGTGAACCGCAGCGCGTTGCGCTGGCTGCAGCGCGGCTGGAACTGAAGCATGTGGT
CATAACATCCGTCACCCGCGACGACCTGCCCGACGGCGGTGCCGCCCACTTTGCAGCCACCATACAGGCCGTGCGCAGCC
GGCTGCCCCGCTGCACGGTGGAAGTGCTCATACCCGACTTTCAGGGAGATGCCGCAGCCCTGCAGACAGTGCTGCAAGCC
CGCCCCGACGTGCTGAACCACAACGTGGAAACCCCGCCCGCGCATTACAGCCGCATACGCCCGCAGGCAGACTATAGTCA
GAGCCTTGAGCTGCTGCGCAGAGCACGCGCAGCCGGTTTTACCGTAAAAAGCGGACTGATGACCGGACTGGGGGAAACGG
ACGCCGAGGTGCTCGGTGTCATTGACGATCTTTGCGCCACGGGCTGCAACATAGTAACCGTGGGGCAGTATATGCGGCCT
TCGCGCAGGCACCCCGCAGTACAGCGTTATGTCCATCCCGACATGTTCGAAGAGTATGCGGCTTACGGACGCGCACGGGG
CATACCGCATATGTTCTGCGCTCCTCTGGTGCGCAGCAGCTACAATGCATCCATGTTCGTGCAGGAAAAAAACTGA

Upstream 100 bases:

>100_bases
CTGTTTGACATGATAACGCTGTGCGGCCTGACAGATGCCCGCGCCACGTCACTGCAGGCCGAATGTAACGATGACAGCAT
CACCCTGCAGGAGGTGAAAG

Downstream 100 bases:

>100_bases
CGGCAGTCAGCCGCACAGGCAGGCATATAACACAACAGCCGGATGTTTTCAGAACATCCGGCTGTCTGCATATCCGCTGT
CGGAAAAAGGTGCGCGGCGC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MSAQENSAPFLRIPPWLRTRIPCNRTYTATRELIGDLNLHTVCQSAKCPNMFECFSSRTATFLILGGTCTRNCAFCNIEP
GDVLPPDAGEPQRVALAAARLELKHVVITSVTRDDLPDGGAAHFAATIQAVRSRLPRCTVEVLIPDFQGDAAALQTVLQA
RPDVLNHNVETPPAHYSRIRPQADYSQSLELLRRARAAGFTVKSGLMTGLGETDAEVLGVIDDLCATGCNIVTVGQYMRP
SRRHPAVQRYVHPDMFEEYAAYGRARGIPHMFCAPLVRSSYNASMFVQEKN

Sequences:

>Translated_291_residues
MSAQENSAPFLRIPPWLRTRIPCNRTYTATRELIGDLNLHTVCQSAKCPNMFECFSSRTATFLILGGTCTRNCAFCNIEP
GDVLPPDAGEPQRVALAAARLELKHVVITSVTRDDLPDGGAAHFAATIQAVRSRLPRCTVEVLIPDFQGDAAALQTVLQA
RPDVLNHNVETPPAHYSRIRPQADYSQSLELLRRARAAGFTVKSGLMTGLGETDAEVLGVIDDLCATGCNIVTVGQYMRP
SRRHPAVQRYVHPDMFEEYAAYGRARGIPHMFCAPLVRSSYNASMFVQEKN
>Mature_290_residues
SAQENSAPFLRIPPWLRTRIPCNRTYTATRELIGDLNLHTVCQSAKCPNMFECFSSRTATFLILGGTCTRNCAFCNIEPG
DVLPPDAGEPQRVALAAARLELKHVVITSVTRDDLPDGGAAHFAATIQAVRSRLPRCTVEVLIPDFQGDAAALQTVLQAR
PDVLNHNVETPPAHYSRIRPQADYSQSLELLRRARAAGFTVKSGLMTGLGETDAEVLGVIDDLCATGCNIVTVGQYMRPS
RRHPAVQRYVHPDMFEEYAAYGRARGIPHMFCAPLVRSSYNASMFVQEKN

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=43.1578947368421, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=44.1295546558704, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=47.1428571428571, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32564533, Length=288, Percent_Identity=39.5833333333333, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=293, Percent_Identity=39.9317406143345, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=40.3508771929825, Blast_Score=228, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_DESDG (Q30XT6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389205.1
- ProteinModelPortal:   Q30XT6
- STRING:   Q30XT6
- GeneID:   3757737
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2714
- NMPDR:   fig|207559.3.peg.3067
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   ARCPNIT
- ProtClustDB:   PRK05481
- BioCyc:   DDES207559:DDE_2714-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32016; Mature: 31885

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQENSAPFLRIPPWLRTRIPCNRTYTATRELIGDLNLHTVCQSAKCPNMFECFSSRTA
CCCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCE
TFLILGGTCTRNCAFCNIEPGDVLPPDAGEPQRVALAAARLELKHVVITSVTRDDLPDGG
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEHHHHCCCCCCC
AAHFAATIQAVRSRLPRCTVEVLIPDFQGDAAALQTVLQARPDVLNHNVETPPAHYSRIR
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCCCHHHHHCC
PQADYSQSLELLRRARAAGFTVKSGLMTGLGETDAEVLGVIDDLCATGCNIVTVGQYMRP
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECHHHCC
SRRHPAVQRYVHPDMFEEYAAYGRARGIPHMFCAPLVRSSYNASMFVQEKN
CCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
SAQENSAPFLRIPPWLRTRIPCNRTYTATRELIGDLNLHTVCQSAKCPNMFECFSSRTA
CCCCCCCCEEECCHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHCCCCE
TFLILGGTCTRNCAFCNIEPGDVLPPDAGEPQRVALAAARLELKHVVITSVTRDDLPDGG
EEEEECCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEHHHHCCCCCCC
AAHFAATIQAVRSRLPRCTVEVLIPDFQGDAAALQTVLQARPDVLNHNVETPPAHYSRIR
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCHHHCCCCCCCCHHHHHCC
PQADYSQSLELLRRARAAGFTVKSGLMTGLGETDAEVLGVIDDLCATGCNIVTVGQYMRP
CCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEECHHHCC
SRRHPAVQRYVHPDMFEEYAAYGRARGIPHMFCAPLVRSSYNASMFVQEKN
CCCCHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA