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The map label for this gene is proB

Identifier: 78357731

GI number: 78357731

Start: 2695769

End: 2696908

Strand: Reverse

Name: proB

Synonym: Dde_2689

Alternate gene names: 78357731

Gene position: 2696908-2695769 (Counterclockwise)

Preceding gene: 161485606

Following gene: 78357730

Centisome position: 72.3

GC content: 61.05

Gene sequence:

>1140_bases
ATGGACTGGCAGACCGAGCGCGGGCAGGTTCTGGATTCCGCCCGCAGAGTGGTTATCAAGGTGGGGAGTGCGGTGCTTAC
TTCCGGCAACGGGCTGGATGTGGGCGTGGTGGATAATCTGGTGGCCCAGATTGCCGACCTGCACGGCCGCGGCGTAGATG
TGGTGCTGGTGTCCTCCGGTGCGGTTTCGGCCGGGCGTGCCGTGCTGCGGCGCTGCTGCGAGATAAAAGGCATGCCGCAC
AAGCAGGCGGCATCGGCCGTGGGGCAGAGCCGTCTCATGCACTATTATGACGAGGCGTTCGGCAGACACGGCATTATTTC
CGCGCAGATACTGCTCACCAAGGACGACCTGCGCAGCCGTCACCGTTTTCTTAACGCCCGCAACACGTTTGCGGCACTTA
TGGACTGGCATGCCGTGCCCATTGTCAATGAAAACGACACAGTGGCCGTACGCGAACTGGAATTCGGCGACAATGACAGT
CTGGCCAGTCTGCTGCTGAATGTGGTGGATGCCGACCTGTTCATCAATCTGACATCGGCCGGCGGAGTATATGCCGACAA
CCCCGACACCAACCCCGATGCGCGTGTCATGGAATGCATAGAAAATGTGCACGGGCTTAATCTGGACGTGATGTGCGGCG
GTAAAACCGCTGTGGGGTCGGGCGGCATGTATTCAAAATTGCTGGCGGCACGGCGGGCAGCACAGCTTGGGGTGCCCACG
CTTATTCTGCCCGGACGCAAGCCCGATGCCATGCTGCGGGCTTTTGCCGGTGACGAACCCGGAACATGGGTGCGACCGGA
AAAGAAAACCGTTTCGGCACGCAAATTCTGGCTGGCGTATCACGCTGATCCCACAGGATCGGTGGTGGTGGATGACGGTG
CTGTAAAAGCGCTTGAGGCGGGCAAAAGCCTGCTGCCTGCCGGAATCATCGGTGTTGAAGGGGGCTTCGGGCGTGGTGCG
CTTGTGCGTGTCACTTCTGCGGCCGGTGCCGTGGTGGCTGTGGGCCTCAGCAACTACCCCGCCGCCGACCTGCGGCGCAT
CATGGGCCACCGCACGGCGGAACTTACGGCCATTCTGGGTGACAACCAGTATCCGGAAGCCATCCACCGCGATAATATGC
TGATGGACGCCGTGGTGTGA

Upstream 100 bases:

>100_bases
TTGATTCTTCGCGCCAGATAGAGCCTGAACCCGAAGAGGAATTTGATGTGGAAGTAGTCTGGGTGCGCGAATAGTCTTTT
AGCGGAGGCGTCTGAAAGCC

Downstream 100 bases:

>100_bases
CCTGCGGCGCAGCGGTCATGATAAAGTGAGGATATATGTCTGAGACTCCGTGTGTTCTTACCATTGCCGGTTCCGATTCC
GGCGGCGGGGCCGGAATTCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MDWQTERGQVLDSARRVVIKVGSAVLTSGNGLDVGVVDNLVAQIADLHGRGVDVVLVSSGAVSAGRAVLRRCCEIKGMPH
KQAASAVGQSRLMHYYDEAFGRHGIISAQILLTKDDLRSRHRFLNARNTFAALMDWHAVPIVNENDTVAVRELEFGDNDS
LASLLLNVVDADLFINLTSAGGVYADNPDTNPDARVMECIENVHGLNLDVMCGGKTAVGSGGMYSKLLAARRAAQLGVPT
LILPGRKPDAMLRAFAGDEPGTWVRPEKKTVSARKFWLAYHADPTGSVVVDDGAVKALEAGKSLLPAGIIGVEGGFGRGA
LVRVTSAAGAVVAVGLSNYPAADLRRIMGHRTAELTAILGDNQYPEAIHRDNMLMDAVV

Sequences:

>Translated_379_residues
MDWQTERGQVLDSARRVVIKVGSAVLTSGNGLDVGVVDNLVAQIADLHGRGVDVVLVSSGAVSAGRAVLRRCCEIKGMPH
KQAASAVGQSRLMHYYDEAFGRHGIISAQILLTKDDLRSRHRFLNARNTFAALMDWHAVPIVNENDTVAVRELEFGDNDS
LASLLLNVVDADLFINLTSAGGVYADNPDTNPDARVMECIENVHGLNLDVMCGGKTAVGSGGMYSKLLAARRAAQLGVPT
LILPGRKPDAMLRAFAGDEPGTWVRPEKKTVSARKFWLAYHADPTGSVVVDDGAVKALEAGKSLLPAGIIGVEGGFGRGA
LVRVTSAAGAVVAVGLSNYPAADLRRIMGHRTAELTAILGDNQYPEAIHRDNMLMDAVV
>Mature_379_residues
MDWQTERGQVLDSARRVVIKVGSAVLTSGNGLDVGVVDNLVAQIADLHGRGVDVVLVSSGAVSAGRAVLRRCCEIKGMPH
KQAASAVGQSRLMHYYDEAFGRHGIISAQILLTKDDLRSRHRFLNARNTFAALMDWHAVPIVNENDTVAVRELEFGDNDS
LASLLLNVVDADLFINLTSAGGVYADNPDTNPDARVMECIENVHGLNLDVMCGGKTAVGSGGMYSKLLAARRAAQLGVPT
LILPGRKPDAMLRAFAGDEPGTWVRPEKKTVSARKFWLAYHADPTGSVVVDDGAVKALEAGKSLLPAGIIGVEGGFGRGA
LVRVTSAAGAVVAVGLSNYPAADLRRIMGHRTAELTAILGDNQYPEAIHRDNMLMDAVV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=303, Percent_Identity=32.3432343234323, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI62912457, Length=301, Percent_Identity=32.2259136212625, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786437, Length=369, Percent_Identity=40.3794037940379, Blast_Score=248, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17569969, Length=330, Percent_Identity=34.8484848484849, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=330, Percent_Identity=34.8484848484849, Blast_Score=139, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=412, Percent_Identity=30.3398058252427, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=28.978622327791, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21357643, Length=302, Percent_Identity=33.4437086092715, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_DESDG (Q30XW1)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389180.1
- ProteinModelPortal:   Q30XW1
- SMR:   Q30XW1
- STRING:   Q30XW1
- GeneID:   3757712
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2689
- NMPDR:   fig|207559.3.peg.3013
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   DDES207559:DDE_2689-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40265; Mature: 40265

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWQTERGQVLDSARRVVIKVGSAVLTSGNGLDVGVVDNLVAQIADLHGRGVDVVLVSSG
CCCCCCHHHHHHHHHHEEEEECCEEEECCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECC
AVSAGRAVLRRCCEIKGMPHKQAASAVGQSRLMHYYDEAFGRHGIISAQILLTKDDLRSR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHH
HRFLNARNTFAALMDWHAVPIVNENDTVAVRELEFGDNDSLASLLLNVVDADLFINLTSA
HHHHCCCHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEECC
GGVYADNPDTNPDARVMECIENVHGLNLDVMCGGKTAVGSGGMYSKLLAARRAAQLGVPT
CCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCE
LILPGRKPDAMLRAFAGDEPGTWVRPEKKTVSARKFWLAYHADPTGSVVVDDGAVKALEA
EEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEECCCCHHHHHH
GKSLLPAGIIGVEGGFGRGALVRVTSAAGAVVAVGLSNYPAADLRRIMGHRTAELTAILG
CCHHCCCCEEECCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEC
DNQYPEAIHRDNMLMDAVV
CCCCCHHHHHCCCHHHHCC
>Mature Secondary Structure
MDWQTERGQVLDSARRVVIKVGSAVLTSGNGLDVGVVDNLVAQIADLHGRGVDVVLVSSG
CCCCCCHHHHHHHHHHEEEEECCEEEECCCCCEEHHHHHHHHHHHHHCCCCEEEEEEECC
AVSAGRAVLRRCCEIKGMPHKQAASAVGQSRLMHYYDEAFGRHGIISAQILLTKDDLRSR
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHH
HRFLNARNTFAALMDWHAVPIVNENDTVAVRELEFGDNDSLASLLLNVVDADLFINLTSA
HHHHCCCHHHHHHHCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEEEEECC
GGVYADNPDTNPDARVMECIENVHGLNLDVMCGGKTAVGSGGMYSKLLAARRAAQLGVPT
CCEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHCCCCE
LILPGRKPDAMLRAFAGDEPGTWVRPEKKTVSARKFWLAYHADPTGSVVVDDGAVKALEA
EEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEECCCCHHHHHH
GKSLLPAGIIGVEGGFGRGALVRVTSAAGAVVAVGLSNYPAADLRRIMGHRTAELTAILG
CCHHCCCCEEECCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHCCCCCEEEEEEC
DNQYPEAIHRDNMLMDAVV
CCCCCHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA