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The map label for this gene is thiDE [H]

Identifier: 78357730

GI number: 78357730

Start: 2694906

End: 2695733

Strand: Reverse

Name: thiDE [H]

Synonym: Dde_2688

Alternate gene names: 78357730

Gene position: 2695733-2694906 (Counterclockwise)

Preceding gene: 78357731

Following gene: 78357729

Centisome position: 72.27

GC content: 60.87

Gene sequence:

>828_bases
ATGTCTGAGACTCCGTGTGTTCTTACCATTGCCGGTTCCGATTCCGGCGGCGGGGCCGGAATTCAGGCCGACCTGAAGAC
CATGACGGTGCTGGGCGGCTTCGGCATGAGTGTCATTACAGCGCTTACCGCGCAGAACGGTCTGGGTGTCACCGGCATTC
ATGCCCCCGATGCTGATTTTGTACGCCTGCAGCTGGAAACGGTGCTGGAAGGCTTTCCCGTGCGCGCTGCAAAGACCGGC
ATGCTTTTTTCTGCGCCTATTATCGAGGCTGTGGCCGATGTGCTGGAAGCTTTGCCGCAGGAGGTACGTTTTCCTCTGGT
GGTGGACCCTGTGTGCGTGAGCCAGAGCGGACACCGTCTGCTGCAGGAGGATGCAGTGGAGGCGCTGAAAAAACGCATGC
TGCCGCTGGCCTATCTGCTGACGCCCAACCGGCCCGAGGCCGAACTGCTGGCCGGTATGGCGGTGGAGACCAAAGAAGAT
GTGGCAGAGGCAATCCGCCGTCTGCTGGATATGGGGCCGCGTGCCGTGCTGCTCAAGGGCGGCCACTTTGAGGGCACCGA
CACCCTGACCGACTGGCTGGGGCTGCCCAACAGAGAGCCTGTGCCCATGCCGCAGACAAAGGTTGATACGCCCAACAACC
ACGGAACAGGTTGCACGCTTTCGGCTGCCATCGCCACTATGCTGGCAAAGGGGCATGTGCTCGACAGGGCCATTCTGAAA
GCCCAGCAGTATCTCAACCTCTGCCTGCGTGAAAGCTATGCCCCCGGTAAGGGGTTCGGACCGCCCAATCATGCGGCAGT
TGTTGCGGGAACACTGTTGCAGGACTGA

Upstream 100 bases:

>100_bases
TGGGTGACAACCAGTATCCGGAAGCCATCCACCGCGATAATATGCTGATGGACGCCGTGGTGTGACCTGCGGCGCAGCGG
TCATGATAAAGTGAGGATAT

Downstream 100 bases:

>100_bases
TTCTGCATTGTTTCTGCGTACTATCGCTGTTGCTAACTATTAAGACCTGCCCTGCAGGTCTTTTTTTATACACAGCGCCG
GAGTGCTTGGAAATGCTGTT

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSETPCVLTIAGSDSGGGAGIQADLKTMTVLGGFGMSVITALTAQNGLGVTGIHAPDADFVRLQLETVLEGFPVRAAKTG
MLFSAPIIEAVADVLEALPQEVRFPLVVDPVCVSQSGHRLLQEDAVEALKKRMLPLAYLLTPNRPEAELLAGMAVETKED
VAEAIRRLLDMGPRAVLLKGGHFEGTDTLTDWLGLPNREPVPMPQTKVDTPNNHGTGCTLSAAIATMLAKGHVLDRAILK
AQQYLNLCLRESYAPGKGFGPPNHAAVVAGTLLQD

Sequences:

>Translated_275_residues
MSETPCVLTIAGSDSGGGAGIQADLKTMTVLGGFGMSVITALTAQNGLGVTGIHAPDADFVRLQLETVLEGFPVRAAKTG
MLFSAPIIEAVADVLEALPQEVRFPLVVDPVCVSQSGHRLLQEDAVEALKKRMLPLAYLLTPNRPEAELLAGMAVETKED
VAEAIRRLLDMGPRAVLLKGGHFEGTDTLTDWLGLPNREPVPMPQTKVDTPNNHGTGCTLSAAIATMLAKGHVLDRAILK
AQQYLNLCLRESYAPGKGFGPPNHAAVVAGTLLQD
>Mature_274_residues
SETPCVLTIAGSDSGGGAGIQADLKTMTVLGGFGMSVITALTAQNGLGVTGIHAPDADFVRLQLETVLEGFPVRAAKTGM
LFSAPIIEAVADVLEALPQEVRFPLVVDPVCVSQSGHRLLQEDAVEALKKRMLPLAYLLTPNRPEAELLAGMAVETKEDV
AEAIRRLLDMGPRAVLLKGGHFEGTDTLTDWLGLPNREPVPMPQTKVDTPNNHGTGCTLSAAIATMLAKGHVLDRAILKA
QQYLNLCLRESYAPGKGFGPPNHAAVVAGTLLQD

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=260, Percent_Identity=41.5384615384615, Blast_Score=168, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6325378, Length=279, Percent_Identity=32.258064516129, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324517, Length=280, Percent_Identity=36.4285714285714, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6324997, Length=283, Percent_Identity=35.3356890459364, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29005; Mature: 28874

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETPCVLTIAGSDSGGGAGIQADLKTMTVLGGFGMSVITALTAQNGLGVTGIHAPDADF
CCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCHH
VRLQLETVLEGFPVRAAKTGMLFSAPIIEAVADVLEALPQEVRFPLVVDPVCVSQSGHRL
HHEEHHHHHCCCCCCHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCHHH
LQEDAVEALKKRMLPLAYLLTPNRPEAELLAGMAVETKEDVAEAIRRLLDMGPRAVLLKG
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEC
GHFEGTDTLTDWLGLPNREPVPMPQTKVDTPNNHGTGCTLSAAIATMLAKGHVLDRAILK
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
AQQYLNLCLRESYAPGKGFGPPNHAAVVAGTLLQD
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SETPCVLTIAGSDSGGGAGIQADLKTMTVLGGFGMSVITALTAQNGLGVTGIHAPDADF
CCCCEEEEEECCCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCCCCHH
VRLQLETVLEGFPVRAAKTGMLFSAPIIEAVADVLEALPQEVRFPLVVDPVCVSQSGHRL
HHEEHHHHHCCCCCCHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECHHHHCCCCHHH
LQEDAVEALKKRMLPLAYLLTPNRPEAELLAGMAVETKEDVAEAIRRLLDMGPRAVLLKG
HHHHHHHHHHHHCCCEEEEECCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEC
GHFEGTDTLTDWLGLPNREPVPMPQTKVDTPNNHGTGCTLSAAIATMLAKGHVLDRAILK
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHH
AQQYLNLCLRESYAPGKGFGPPNHAAVVAGTLLQD
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA