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The map label for this gene is metF [H]

Identifier: 78357568

GI number: 78357568

Start: 2545720

End: 2546670

Strand: Reverse

Name: metF [H]

Synonym: Dde_2525

Alternate gene names: 78357568

Gene position: 2546670-2545720 (Counterclockwise)

Preceding gene: 78357569

Following gene: 78357565

Centisome position: 68.27

GC content: 56.57

Gene sequence:

>951_bases
ATGCATCCCGCCGGCGTAACGTGGCAGCATACAACAGCAACGGCGCATCTGCGCAGTAACAAGGATTCAGACGTGAAAAT
CAAAGACCTGATCGGACAGCATGACGGCCCGTTTTATTCTCTGGAATATTTTCCTCCGAAAAATCCGGAGATGTGGCCGA
AATTTTTTGCCAACGTCGGCAGGCTGATGGCTCTGAACCCTGTGTTCTGCTCCGTGACTTATGGCGCGGGCGGGGGCACG
CAGGACAACACGTTCGAGATTACCCGCCGGTTCAAGCAGGACCTTGATCTGGAACCCATGCCGCACCTGACCTGCGTGGG
CGCCACCAAAGATAAGATACGCGGCTTTGTCAGTCAGCTGAGAGATATCGGCGTGGACAATGTGCTTGCCCTGCGCGGAG
ATGCTCCGGCTGATGCCGGTTTCAGCTGGGACGACGGTGAGTTCAGACATGCTTCCGACCTTGTGGAGTTTGTGAACGCC
GAGTTTCCGGAAATGTGTGTGGGTGTTGCCGGATATCCCACCGCACATCCGGAATCTGCCAGCTTTGCAGAGGATCTGCA
CTGGACAAAGGCGAAAATTGACGCGGGCAGCGATTTCATTGTCTGCCAGCTGCTGTTTGATGTACGCGAATATTTTCATT
TTGTGGCCCAGTTGCGGGCCATGGGGGTGACCAGTCCTGTTCTGCCCGGTATTCTGCCTATCCAGTCTCTCGAGTCGGTG
AGGCGTATTCTTTCGTTGTGCGGAGCCAATATTCCCGGCAGATTTTATCTGCAGCTGGAAGAGGCCGACGCCAAGGGCGG
GGCGGCCGCCGTGAAAGAACTGGGCACAAAGTTCGCCGCGGATATGATCCGGCGGCTGGTGGACGGCGGGGTACCCGGTG
TGCACCTGTATACACTGAATCAGGCGGAAACCTGCCTGCGTATAGCCGAAATGGCAGGCCCGCTGCGATAA

Upstream 100 bases:

>100_bases
TGATTGTCCACCCCGCGCGGGGGTAAAGTAAATATTACAATAGGTCGATATTGACATATAAGGCTATCTTGATATATCGT
TCGTTCATGAGGGACGCACT

Downstream 100 bases:

>100_bases
AACATGCTTTGTGCCGCTGTTCCTTCAGCGGGCTGATCTGGTACAGCTGAAATGAAATCCGGCTCCGTTTTTAAAACGGA
GCCGGATTTTTTGTGCTCTG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MHPAGVTWQHTTATAHLRSNKDSDVKIKDLIGQHDGPFYSLEYFPPKNPEMWPKFFANVGRLMALNPVFCSVTYGAGGGT
QDNTFEITRRFKQDLDLEPMPHLTCVGATKDKIRGFVSQLRDIGVDNVLALRGDAPADAGFSWDDGEFRHASDLVEFVNA
EFPEMCVGVAGYPTAHPESASFAEDLHWTKAKIDAGSDFIVCQLLFDVREYFHFVAQLRAMGVTSPVLPGILPIQSLESV
RRILSLCGANIPGRFYLQLEEADAKGGAAAVKELGTKFAADMIRRLVDGGVPGVHLYTLNQAETCLRIAEMAGPLR

Sequences:

>Translated_316_residues
MHPAGVTWQHTTATAHLRSNKDSDVKIKDLIGQHDGPFYSLEYFPPKNPEMWPKFFANVGRLMALNPVFCSVTYGAGGGT
QDNTFEITRRFKQDLDLEPMPHLTCVGATKDKIRGFVSQLRDIGVDNVLALRGDAPADAGFSWDDGEFRHASDLVEFVNA
EFPEMCVGVAGYPTAHPESASFAEDLHWTKAKIDAGSDFIVCQLLFDVREYFHFVAQLRAMGVTSPVLPGILPIQSLESV
RRILSLCGANIPGRFYLQLEEADAKGGAAAVKELGTKFAADMIRRLVDGGVPGVHLYTLNQAETCLRIAEMAGPLR
>Mature_316_residues
MHPAGVTWQHTTATAHLRSNKDSDVKIKDLIGQHDGPFYSLEYFPPKNPEMWPKFFANVGRLMALNPVFCSVTYGAGGGT
QDNTFEITRRFKQDLDLEPMPHLTCVGATKDKIRGFVSQLRDIGVDNVLALRGDAPADAGFSWDDGEFRHASDLVEFVNA
EFPEMCVGVAGYPTAHPESASFAEDLHWTKAKIDAGSDFIVCQLLFDVREYFHFVAQLRAMGVTSPVLPGILPIQSLESV
RRILSLCGANIPGRFYLQLEEADAKGGAAAVKELGTKFAADMIRRLVDGGVPGVHLYTLNQAETCLRIAEMAGPLR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=32.9787234042553, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=35.036496350365, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI25147436, Length=295, Percent_Identity=33.8983050847458, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI25147441, Length=295, Percent_Identity=33.8983050847458, Blast_Score=167, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6325234, Length=308, Percent_Identity=32.4675324675325, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321313, Length=298, Percent_Identity=31.5436241610738, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24662658, Length=220, Percent_Identity=25.4545454545455, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34685; Mature: 34685

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPAGVTWQHTTATAHLRSNKDSDVKIKDLIGQHDGPFYSLEYFPPKNPEMWPKFFANVG
CCCCCCEEECCCEEEEECCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHH
RLMALNPVFCSVTYGAGGGTQDNTFEITRRFKQDLDLEPMPHLTCVGATKDKIRGFVSQL
HHHEECCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHH
RDIGVDNVLALRGDAPADAGFSWDDGEFRHASDLVEFVNAEFPEMCVGVAGYPTAHPESA
HHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
SFAEDLHWTKAKIDAGSDFIVCQLLFDVREYFHFVAQLRAMGVTSPVLPGILPIQSLESV
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHCHHHHHHH
RRILSLCGANIPGRFYLQLEEADAKGGAAAVKELGTKFAADMIRRLVDGGVPGVHLYTLN
HHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
QAETCLRIAEMAGPLR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHPAGVTWQHTTATAHLRSNKDSDVKIKDLIGQHDGPFYSLEYFPPKNPEMWPKFFANVG
CCCCCCEEECCCEEEEECCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHH
RLMALNPVFCSVTYGAGGGTQDNTFEITRRFKQDLDLEPMPHLTCVGATKDKIRGFVSQL
HHHEECCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHH
RDIGVDNVLALRGDAPADAGFSWDDGEFRHASDLVEFVNAEFPEMCVGVAGYPTAHPESA
HHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCC
SFAEDLHWTKAKIDAGSDFIVCQLLFDVREYFHFVAQLRAMGVTSPVLPGILPIQSLESV
HHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHCHHHHHHH
RRILSLCGANIPGRFYLQLEEADAKGGAAAVKELGTKFAADMIRRLVDGGVPGVHLYTLN
HHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
QAETCLRIAEMAGPLR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]