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The map label for this gene is glyA

Identifier: 78357475

GI number: 78357475

Start: 2453220

End: 2454458

Strand: Direct

Name: glyA

Synonym: Dde_2432

Alternate gene names: 78357475

Gene position: 2453220-2454458 (Clockwise)

Preceding gene: 78357474

Following gene: 78357476

Centisome position: 65.77

GC content: 60.61

Gene sequence:

>1239_bases
ATGGACGAACTGCTTATTCAGGATCCGGAAGTCGGTCGCGCCATTGTTCAGGAAGTGGAACGTCAGACCGGCAAGCTGGA
ACTCATCGCCTCTGAAAACTTTGTGTCCCCCGCAGTCCGCGCCGCACAGGGCAGCGTGCTGACCCATAAATACGCCGAAG
GATACCCCGGCAAGCGGTATTACGGCGGTTGCGAATTTGTCGACGTGGCGGAAAACCTCGCCATCGACCGCGCCTGCGAA
ATATTCGGCGCCCAGTACGCCAACGTCCAGCCCCACTCCGGCAGCCAGGCCAATATGGCCGTATACTTTTCCGCGCTGAC
TCCCGGCGACACCATTCTGGCCATGGATCTGTCGCACGGCGGACACCTGACCCACGGCAGCCCCGTCAACTTTTCGGGCC
GGTTCTACAATGTGGTGTTCTACGGTGTCTCGCGCGAAACCGGCTGCATAGATTATGACAGCGTGGCAGAACTGGCACGC
GAGCACCGCCCCGCCATGATTGTGGCCGGTGCCAGCGCCTACTCCCGCATCATCGATTTTGCACGGTTCCGCGCCATAGC
CGACGAAGTGGGTTCCCTGCTTATGGTCGACATGGCCCACATAGCCGGTCTGGTGGCCGCCGGTCTGCATCCTTCCCCTG
TGGGCACGGCGCACTTCACCACCACAACCACGCACAAAACCCTGCGCGGTCCGCGTGGCGGCATGATTCTGTCGGACGAA
GAAGCCGCCAAAAAACTGAACAGCCAGATTTTCCCCGGCATTCAGGGCGGCCCGCTCATGCATGTCATCGCCGCCAAGGC
TGTGGCCTTCGGCGAAGCGCTGCGTCCCGAATTCGGCGCCTACCAGAAGCAGGTGGTCGCCAACGCCGCCAAACTGGCCG
CCACCCTGACCGATGCCGGTTTTGAACTGGTTTCCGGCGGCACCGACAACCACCTGATGCTGGTGGACCTGACAAACAAG
GATATCACCGGTAAAGATGCCCAGCATGCGCTTGACCTTGCGGGCATCACCGCCAACAAAAACACGGTGCCTTTTGAAAC
CCGCTCGCCTTTCGTGACTTCGGGCATCCGGCTGGGTACCCCTGCCCTGACCACCCGCGGCATGAAAGAAGCTGAAATGG
TCAAAGTGGCCGGCTGGATAATCGACGCGCTGGGCAATATCGGCAACGAAACCCGTCTGGCGGAAATAAGCCGCGACGTG
GAAAAGTTCGCCCGTCAGTTCCCGCTGTTCCACTGGTAA

Upstream 100 bases:

>100_bases
TGCATGCTGTTCAAGCGCTGGGAAGGCTAATACCGAATCCCTCCGCCGCGCATCCCCCTGCGGGGTGCGCGGCATAACCA
CAACGCGGAGAGTGGAAACC

Downstream 100 bases:

>100_bases
CAGCAGACAAAGCAACATTGCAGCGGGGCGGTTTGACAAAACCGCCCCGCTATATTCACTGCTAAAGCCGCACACAGCGC
GGCACACGGATATCACGCAT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MDELLIQDPEVGRAIVQEVERQTGKLELIASENFVSPAVRAAQGSVLTHKYAEGYPGKRYYGGCEFVDVAENLAIDRACE
IFGAQYANVQPHSGSQANMAVYFSALTPGDTILAMDLSHGGHLTHGSPVNFSGRFYNVVFYGVSRETGCIDYDSVAELAR
EHRPAMIVAGASAYSRIIDFARFRAIADEVGSLLMVDMAHIAGLVAAGLHPSPVGTAHFTTTTTHKTLRGPRGGMILSDE
EAAKKLNSQIFPGIQGGPLMHVIAAKAVAFGEALRPEFGAYQKQVVANAAKLAATLTDAGFELVSGGTDNHLMLVDLTNK
DITGKDAQHALDLAGITANKNTVPFETRSPFVTSGIRLGTPALTTRGMKEAEMVKVAGWIIDALGNIGNETRLAEISRDV
EKFARQFPLFHW

Sequences:

>Translated_412_residues
MDELLIQDPEVGRAIVQEVERQTGKLELIASENFVSPAVRAAQGSVLTHKYAEGYPGKRYYGGCEFVDVAENLAIDRACE
IFGAQYANVQPHSGSQANMAVYFSALTPGDTILAMDLSHGGHLTHGSPVNFSGRFYNVVFYGVSRETGCIDYDSVAELAR
EHRPAMIVAGASAYSRIIDFARFRAIADEVGSLLMVDMAHIAGLVAAGLHPSPVGTAHFTTTTTHKTLRGPRGGMILSDE
EAAKKLNSQIFPGIQGGPLMHVIAAKAVAFGEALRPEFGAYQKQVVANAAKLAATLTDAGFELVSGGTDNHLMLVDLTNK
DITGKDAQHALDLAGITANKNTVPFETRSPFVTSGIRLGTPALTTRGMKEAEMVKVAGWIIDALGNIGNETRLAEISRDV
EKFARQFPLFHW
>Mature_412_residues
MDELLIQDPEVGRAIVQEVERQTGKLELIASENFVSPAVRAAQGSVLTHKYAEGYPGKRYYGGCEFVDVAENLAIDRACE
IFGAQYANVQPHSGSQANMAVYFSALTPGDTILAMDLSHGGHLTHGSPVNFSGRFYNVVFYGVSRETGCIDYDSVAELAR
EHRPAMIVAGASAYSRIIDFARFRAIADEVGSLLMVDMAHIAGLVAAGLHPSPVGTAHFTTTTTHKTLRGPRGGMILSDE
EAAKKLNSQIFPGIQGGPLMHVIAAKAVAFGEALRPEFGAYQKQVVANAAKLAATLTDAGFELVSGGTDNHLMLVDLTNK
DITGKDAQHALDLAGITANKNTVPFETRSPFVTSGIRLGTPALTTRGMKEAEMVKVAGWIIDALGNIGNETRLAEISRDV
EKFARQFPLFHW

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=448, Percent_Identity=45.9821428571429, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862350, Length=448, Percent_Identity=45.9821428571429, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862348, Length=448, Percent_Identity=45.9821428571429, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI19923315, Length=448, Percent_Identity=45.9821428571429, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=44.8660714285714, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI22547186, Length=460, Percent_Identity=45.6521739130435, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI22547189, Length=445, Percent_Identity=44.2696629213483, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1788902, Length=406, Percent_Identity=60.5911330049261, Blast_Score=488, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=47.6426799007444, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=47.6426799007444, Blast_Score=340, Evalue=6e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=46.1346633416459, Blast_Score=350, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=407, Percent_Identity=43.7346437346437, Blast_Score=319, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24640005, Length=460, Percent_Identity=45, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI221329721, Length=460, Percent_Identity=45, Blast_Score=372, Evalue=1e-103,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_DESDG (Q30YL7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388924.1
- ProteinModelPortal:   Q30YL7
- SMR:   Q30YL7
- STRING:   Q30YL7
- GeneID:   3757448
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2432
- NMPDR:   fig|207559.3.peg.2790
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   NIGNETR
- ProtClustDB:   PRK00011
- BioCyc:   DDES207559:DDE_2432-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 44218; Mature: 44218

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELLIQDPEVGRAIVQEVERQTGKLELIASENFVSPAVRAAQGSVLTHKYAEGYPGKRY
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCEEEEHHHCCCCCCCC
YGGCEFVDVAENLAIDRACEIFGAQYANVQPHSGSQANMAVYFSALTPGDTILAMDLSHG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGRFYNVVFYGVSRETGCIDYDSVAELAREHRPAMIVAGASAYSRIIDF
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHH
ARFRAIADEVGSLLMVDMAHIAGLVAAGLHPSPVGTAHFTTTTTHKTLRGPRGGMILSDE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHCCCCCCEEECCH
EAAKKLNSQIFPGIQGGPLMHVIAAKAVAFGEALRPEFGAYQKQVVANAAKLAATLTDAG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
FELVSGGTDNHLMLVDLTNKDITGKDAQHALDLAGITANKNTVPFETRSPFVTSGIRLGT
HHEECCCCCCEEEEEEECCCCCCCCCHHHHHHHEEEECCCCCCCCCCCCCHHCCCEECCC
PALTTRGMKEAEMVKVAGWIIDALGNIGNETRLAEISRDVEKFARQFPLFHW
CHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDELLIQDPEVGRAIVQEVERQTGKLELIASENFVSPAVRAAQGSVLTHKYAEGYPGKRY
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCEEEEHHHCCCCCCCC
YGGCEFVDVAENLAIDRACEIFGAQYANVQPHSGSQANMAVYFSALTPGDTILAMDLSHG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEECCC
GHLTHGSPVNFSGRFYNVVFYGVSRETGCIDYDSVAELAREHRPAMIVAGASAYSRIIDF
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHH
ARFRAIADEVGSLLMVDMAHIAGLVAAGLHPSPVGTAHFTTTTTHKTLRGPRGGMILSDE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHCCCCCCEEECCH
EAAKKLNSQIFPGIQGGPLMHVIAAKAVAFGEALRPEFGAYQKQVVANAAKLAATLTDAG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
FELVSGGTDNHLMLVDLTNKDITGKDAQHALDLAGITANKNTVPFETRSPFVTSGIRLGT
HHEECCCCCCEEEEEEECCCCCCCCCHHHHHHHEEEECCCCCCCCCCCCCHHCCCEECCC
PALTTRGMKEAEMVKVAGWIIDALGNIGNETRLAEISRDVEKFARQFPLFHW
CHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA