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The map label for this gene is nfo

Identifier: 78357445

GI number: 78357445

Start: 2423710

End: 2424594

Strand: Reverse

Name: nfo

Synonym: Dde_2402

Alternate gene names: 78357445

Gene position: 2424594-2423710 (Counterclockwise)

Preceding gene: 78357448

Following gene: 78357443

Centisome position: 65.0

GC content: 62.49

Gene sequence:

>885_bases
ATGGAAACTATATCACGTCAGAACAAGCCGCAACAGCAGTTGATCGGGGCGCATATGTCCGCGGCGGGCGGCGTGCACAA
GGCCGTTGAGCGCGCAGAGGCAGCAGGAGCCACAGCGCTGCAGGTGTTTACACGCAACCAGCGTCAGTGGAACGCCCCGC
CGCTGGAAGAGCATGACGCAGAGCTGTTTACGGCGGCCGTGCGCCGCTGGGGCGGATATCCGGTATCGTCACATGCATCG
TATCTGATAAACCCCGCTGCGGACACGCCGGAAGGGGCTGAGCGCTCGGTGGCGCTGATGGCTGACGAACTGCAGCGTGC
GGGCAGACTGGGCATTGAAATGGTTGTGGTGCACCCCGGAGCGCATAAAGGACAGGGAGAAGCAGCCGGTGCCGCGCTGG
CGGCGGCGCGTCTGGATGAAGCGCTGGAACGCTGCGGCAGTCCCGCGGTTATGGTGCTGCTGGAAAACACGGCGGGGCAG
GGCACCATGCTGGGGGCGTCATTTGCCGGACTGGCGGCTGTCATTGAAGCTTCGCGCATGCCGGAACGCTATGGCATATG
CCTTGATACGGCGCATGCATTCGGTGCCGGATATGATCTGCGAGACGCGGCATTATATGATGCTGCCATACGTGAACTGG
ATGTCTGTGCGGGACTGGACAGGCTGCGGCTGGTGCATGCCAACGACTCGCTGACCGGACTGGGCAGCCGCAGAGACAGG
CATACCCACATAGGGCAGGGAGAACTGGGCGAAGCGGCTTTCCGCCTGCTGATGCGTGACGAGCGGCTGCACCATGTGCC
CAAGGTGCTTGAAACCCCCAAGGGAAAAGGGCCGGAAGAAGACATGATGAATATGGCTGTGCTCCGGAGGCTTGCCTGCC
CCTAG

Upstream 100 bases:

>100_bases
TCAATTATATTTTGGGCACTGTACTGTGTTCCATACTTTGAGGAAATGAGGGGTTTCCCTCAGTACAGGGTAGGGAAAGT
CATATACCCACAGGTGGATT

Downstream 100 bases:

>100_bases
CATGCCGGTGTGCCTGCAGAAAAGGCGGGATGCGCGTTGCGGTGTAAAAAAAACGCGCCGCGTGGTGACGCGGCGCGGAT
GATTTTTTCCGGTGCAGGCG

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
METISRQNKPQQQLIGAHMSAAGGVHKAVERAEAAGATALQVFTRNQRQWNAPPLEEHDAELFTAAVRRWGGYPVSSHAS
YLINPAADTPEGAERSVALMADELQRAGRLGIEMVVVHPGAHKGQGEAAGAALAAARLDEALERCGSPAVMVLLENTAGQ
GTMLGASFAGLAAVIEASRMPERYGICLDTAHAFGAGYDLRDAALYDAAIRELDVCAGLDRLRLVHANDSLTGLGSRRDR
HTHIGQGELGEAAFRLLMRDERLHHVPKVLETPKGKGPEEDMMNMAVLRRLACP

Sequences:

>Translated_294_residues
METISRQNKPQQQLIGAHMSAAGGVHKAVERAEAAGATALQVFTRNQRQWNAPPLEEHDAELFTAAVRRWGGYPVSSHAS
YLINPAADTPEGAERSVALMADELQRAGRLGIEMVVVHPGAHKGQGEAAGAALAAARLDEALERCGSPAVMVLLENTAGQ
GTMLGASFAGLAAVIEASRMPERYGICLDTAHAFGAGYDLRDAALYDAAIRELDVCAGLDRLRLVHANDSLTGLGSRRDR
HTHIGQGELGEAAFRLLMRDERLHHVPKVLETPKGKGPEEDMMNMAVLRRLACP
>Mature_294_residues
METISRQNKPQQQLIGAHMSAAGGVHKAVERAEAAGATALQVFTRNQRQWNAPPLEEHDAELFTAAVRRWGGYPVSSHAS
YLINPAADTPEGAERSVALMADELQRAGRLGIEMVVVHPGAHKGQGEAAGAALAAARLDEALERCGSPAVMVLLENTAGQ
GTMLGASFAGLAAVIEASRMPERYGICLDTAHAFGAGYDLRDAALYDAAIRELDVCAGLDRLRLVHANDSLTGLGSRRDR
HTHIGQGELGEAAFRLLMRDERLHHVPKVLETPKGKGPEEDMMNMAVLRRLACP

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=263, Percent_Identity=41.06463878327, Blast_Score=194, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17531193, Length=282, Percent_Identity=39.3617021276596, Blast_Score=220, Evalue=6e-58,
Organism=Saccharomyces cerevisiae, GI6322735, Length=277, Percent_Identity=34.6570397111913, Blast_Score=168, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_DESDG (Q30YP7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388894.1
- ProteinModelPortal:   Q30YP7
- SMR:   Q30YP7
- STRING:   Q30YP7
- GeneID:   3757418
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2402
- NMPDR:   fig|207559.3.peg.2760
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- BioCyc:   DDES207559:DDE_2402-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31475; Mature: 31475

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METISRQNKPQQQLIGAHMSAAGGVHKAVERAEAAGATALQVFTRNQRQWNAPPLEEHDA
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHH
ELFTAAVRRWGGYPVSSHASYLINPAADTPEGAERSVALMADELQRAGRLGIEMVVVHPG
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
AHKGQGEAAGAALAAARLDEALERCGSPAVMVLLENTAGQGTMLGASFAGLAAVIEASRM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
PERYGICLDTAHAFGAGYDLRDAALYDAAIRELDVCAGLDRLRLVHANDSLTGLGSRRDR
HHHHCCEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
HTHIGQGELGEAAFRLLMRDERLHHVPKVLETPKGKGPEEDMMNMAVLRRLACP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METISRQNKPQQQLIGAHMSAAGGVHKAVERAEAAGATALQVFTRNQRQWNAPPLEEHDA
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHH
ELFTAAVRRWGGYPVSSHASYLINPAADTPEGAERSVALMADELQRAGRLGIEMVVVHPG
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCC
AHKGQGEAAGAALAAARLDEALERCGSPAVMVLLENTAGQGTMLGASFAGLAAVIEASRM
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
PERYGICLDTAHAFGAGYDLRDAALYDAAIRELDVCAGLDRLRLVHANDSLTGLGSRRDR
HHHHCCEEECHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCC
HTHIGQGELGEAAFRLLMRDERLHHVPKVLETPKGKGPEEDMMNMAVLRRLACP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA