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The map label for this gene is trmE

Identifier: 78357435

GI number: 78357435

Start: 2415283

End: 2416689

Strand: Reverse

Name: trmE

Synonym: Dde_2392

Alternate gene names: 78357435

Gene position: 2416689-2415283 (Counterclockwise)

Preceding gene: 78357436

Following gene: 78357434

Centisome position: 64.79

GC content: 61.41

Gene sequence:

>1407_bases
ATGTCACAGCATCACGGAACAGACACCATAGCCGCCGTCGCCACCCCGCTGGGGCAGGGCGGGGTGGGCATCATCCGGCT
GAGCGGTCCGCGCAGCCATGCCATACTTACAGCCCTGTTCGACTCCACATCGCCCGGTTTCAGCGGTTTCCGACCGCGGG
TGCTGCATCATGGGCACATTCATGACGGTACGCCGCAGCGCAGGATTCTGGACGATGTGCTGGCGGTGCACATGCCTGCA
CCGCGTACGTTTACCGGTGAAGATGTGGTGGAAATCAACTGCCATGGCGGACCTGCCATTCTTGCCGCGGTGCTGGATGC
GGTGTTGCGTCAGGGCGCACGGCCCGCGGCGCACGGCGAATTTACCCGACGGGCTTTTCTTAACGGTCGCATGGACCTGA
CGCAGGCTGAGGCTGTGGCCGAGATGATAGCGGCCCCGGCGGCGGAAGGCGTCCGGCTGGCGCAGGCCCGTCTTGACGGC
ATGCTTGGCAGACACATAAACGCCCTGCGCGAACGGCTTGAACTGGTACGTGTGCAGTTGTGTGTGGCGGTCGATTTTCC
AGAGGATGAAGTGGAATGTCTTGCCCCGGCCGATTTTATGCACGCAGTTGAAGAAACTGCGGATGCTATCCGCAAGCTTA
TCGGTAACTACGAGCGGGCCCGCTGCTGGCGTGAAGGAGCTCAGGTGGTGCTGGCCGGACAGGTGAATGCCGGCAAATCA
AGTCTGCTTAATGCCCTGCTGGGCAGAAAGCGGGCCATAGTGACGGACGTTCCCGGCACCACACGTGATTTTCTGGAAGA
AAGCATTACGCTGGACGGGCTGGCCGTGCGTCTGGTTGATACCGCCGGCCTGCGCGAGACAGGCGACATCGTGGAGCAGG
AAGGTGTGCGCATGAGCCGTGACCTTGCCGCTCAGGCTGATCTGGTGCTGCTGGTGGTGGATTCCCGTTTGGGCACCGGC
CCGCATGAGCAGGAACTTATGGCCGCCATGGAAGCCGGATCTGTACTTGTTGTGCTGAACAAGACCGATGCCGCGACCGA
GGATGAACTGGACAGGTGTGTCCGGTCACTTGCCGGTCGCCCTCATGTGGCTGTATCTGCCCGTGAAGGAAAGGGGATGG
ATTCTCTGGCCGCAGCCGTGCGCGAGATGGTGCTGCGCCGCAGCAACGCGCTGACCGCAGATGCTGCCGCATCGGATATG
GCACCCAACCTGCGCCAGACGGAAGCCCTGCGGCGCGCGCTTGAAGAGCTGGAAGCGCTGGCTGCCGATATCGTTTCGGG
AGTGCCGTATGACCTTGCGGGAGTCCGCCTTGAGTCTGCCTGCTCCGTTCTTTCAGAAATCACGGGCGAAACCACGCCGG
ATGATATTCTGAACAGGATTTTTGATTCGTTCTGCATCGGCAAGTGA

Upstream 100 bases:

>100_bases
CTTTCTATGTATAGTCAGCAGGTACGCCCGTGCCGTGCCGCAACGGTTTATTTCCGGCGTCCATTCAACGCCGGTTCATT
TTTTTCAGGAACACAGCATC

Downstream 100 bases:

>100_bases
TTCTGCCGCCGTGCAAGCCCGTCCGTGCAGGACGGGCTTTTTTTGTGTGCAGACAATGTGACCTGCATCACAGACAAGGC
TCTGCCGGGCGATACACTGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSQHHGTDTIAAVATPLGQGGVGIIRLSGPRSHAILTALFDSTSPGFSGFRPRVLHHGHIHDGTPQRRILDDVLAVHMPA
PRTFTGEDVVEINCHGGPAILAAVLDAVLRQGARPAAHGEFTRRAFLNGRMDLTQAEAVAEMIAAPAAEGVRLAQARLDG
MLGRHINALRERLELVRVQLCVAVDFPEDEVECLAPADFMHAVEETADAIRKLIGNYERARCWREGAQVVLAGQVNAGKS
SLLNALLGRKRAIVTDVPGTTRDFLEESITLDGLAVRLVDTAGLRETGDIVEQEGVRMSRDLAAQADLVLLVVDSRLGTG
PHEQELMAAMEAGSVLVVLNKTDAATEDELDRCVRSLAGRPHVAVSAREGKGMDSLAAAVREMVLRRSNALTADAAASDM
APNLRQTEALRRALEELEALAADIVSGVPYDLAGVRLESACSVLSEITGETTPDDILNRIFDSFCIGK

Sequences:

>Translated_468_residues
MSQHHGTDTIAAVATPLGQGGVGIIRLSGPRSHAILTALFDSTSPGFSGFRPRVLHHGHIHDGTPQRRILDDVLAVHMPA
PRTFTGEDVVEINCHGGPAILAAVLDAVLRQGARPAAHGEFTRRAFLNGRMDLTQAEAVAEMIAAPAAEGVRLAQARLDG
MLGRHINALRERLELVRVQLCVAVDFPEDEVECLAPADFMHAVEETADAIRKLIGNYERARCWREGAQVVLAGQVNAGKS
SLLNALLGRKRAIVTDVPGTTRDFLEESITLDGLAVRLVDTAGLRETGDIVEQEGVRMSRDLAAQADLVLLVVDSRLGTG
PHEQELMAAMEAGSVLVVLNKTDAATEDELDRCVRSLAGRPHVAVSAREGKGMDSLAAAVREMVLRRSNALTADAAASDM
APNLRQTEALRRALEELEALAADIVSGVPYDLAGVRLESACSVLSEITGETTPDDILNRIFDSFCIGK
>Mature_467_residues
SQHHGTDTIAAVATPLGQGGVGIIRLSGPRSHAILTALFDSTSPGFSGFRPRVLHHGHIHDGTPQRRILDDVLAVHMPAP
RTFTGEDVVEINCHGGPAILAAVLDAVLRQGARPAAHGEFTRRAFLNGRMDLTQAEAVAEMIAAPAAEGVRLAQARLDGM
LGRHINALRERLELVRVQLCVAVDFPEDEVECLAPADFMHAVEETADAIRKLIGNYERARCWREGAQVVLAGQVNAGKSS
LLNALLGRKRAIVTDVPGTTRDFLEESITLDGLAVRLVDTAGLRETGDIVEQEGVRMSRDLAAQADLVLLVVDSRLGTGP
HEQELMAAMEAGSVLVVLNKTDAATEDELDRCVRSLAGRPHVAVSAREGKGMDSLAAAVREMVLRRSNALTADAAASDMA
PNLRQTEALRRALEELEALAADIVSGVPYDLAGVRLESACSVLSEITGETTPDDILNRIFDSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=487, Percent_Identity=34.7022587268994, Blast_Score=198, Evalue=9e-51,
Organism=Homo sapiens, GI193082993, Length=476, Percent_Identity=34.453781512605, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI193082988, Length=512, Percent_Identity=33.0078125, Blast_Score=186, Evalue=5e-47,
Organism=Escherichia coli, GI2367268, Length=472, Percent_Identity=39.4067796610169, Blast_Score=265, Evalue=5e-72,
Organism=Escherichia coli, GI87082120, Length=168, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=477, Percent_Identity=28.9308176100629, Blast_Score=175, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=509, Percent_Identity=30.0589390962672, Blast_Score=212, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45550813, Length=491, Percent_Identity=31.3645621181263, Blast_Score=194, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_DESDG (Q30YQ7)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388884.1
- HSSP:   Q9WYA4
- ProteinModelPortal:   Q30YQ7
- SMR:   Q30YQ7
- STRING:   Q30YQ7
- GeneID:   3757408
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2392
- NMPDR:   fig|207559.3.peg.2750
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   DDES207559:DDE_2392-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50008; Mature: 49876

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: BINDING 26-26 BINDING 91-91 BINDING 130-130 BINDING 468-468

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHHGTDTIAAVATPLGQGGVGIIRLSGPRSHAILTALFDSTSPGFSGFRPRVLHHGHI
CCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHEECCCCCCCCCCCCCHHEECCCC
HDGTPQRRILDDVLAVHMPAPRTFTGEDVVEINCHGGPAILAAVLDAVLRQGARPAAHGE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCH
FTRRAFLNGRMDLTQAEAVAEMIAAPAAEGVRLAQARLDGMLGRHINALRERLELVRVQL
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVAVDFPEDEVECLAPADFMHAVEETADAIRKLIGNYERARCWREGAQVVLAGQVNAGKS
HEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCHH
SLLNALLGRKRAIVTDVPGTTRDFLEESITLDGLAVRLVDTAGLRETGDIVEQEGVRMSR
HHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHH
DLAAQADLVLLVVDSRLGTGPHEQELMAAMEAGSVLVVLNKTDAATEDELDRCVRSLAGR
HHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCC
PHVAVSAREGKGMDSLAAAVREMVLRRSNALTADAAASDMAPNLRQTEALRRALEELEAL
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
AADIVSGVPYDLAGVRLESACSVLSEITGETTPDDILNRIFDSFCIGK
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQHHGTDTIAAVATPLGQGGVGIIRLSGPRSHAILTALFDSTSPGFSGFRPRVLHHGHI
CCCCCCHHHHHHHCCCCCCCEEEEEECCCCCHHHHHEECCCCCCCCCCCCCHHEECCCC
HDGTPQRRILDDVLAVHMPAPRTFTGEDVVEINCHGGPAILAAVLDAVLRQGARPAAHGE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCH
FTRRAFLNGRMDLTQAEAVAEMIAAPAAEGVRLAQARLDGMLGRHINALRERLELVRVQL
HHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVAVDFPEDEVECLAPADFMHAVEETADAIRKLIGNYERARCWREGAQVVLAGQVNAGKS
HEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCCCCHH
SLLNALLGRKRAIVTDVPGTTRDFLEESITLDGLAVRLVDTAGLRETGDIVEQEGVRMSR
HHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHH
DLAAQADLVLLVVDSRLGTGPHEQELMAAMEAGSVLVVLNKTDAATEDELDRCVRSLAGR
HHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCC
PHVAVSAREGKGMDSLAAAVREMVLRRSNALTADAAASDMAPNLRQTEALRRALEELEAL
CCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHH
AADIVSGVPYDLAGVRLESACSVLSEITGETTPDDILNRIFDSFCIGK
HHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA