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The map label for this gene is rlmE

Identifier: 78357369

GI number: 78357369

Start: 2342912

End: 2343535

Strand: Reverse

Name: rlmE

Synonym: Dde_2326

Alternate gene names: 78357369

Gene position: 2343535-2342912 (Counterclockwise)

Preceding gene: 78357371

Following gene: 78357368

Centisome position: 62.83

GC content: 51.76

Gene sequence:

>624_bases
ATGAAAAAATATCGTGACCATTATTTTCTGAAGGCCAAACGGGAGAATTATCCCGCGCGGTCTGTCTATAAGCTGAAAGA
AATAGATAAACGCTTCGGCATCTTGAAGCAAGGCATGCGTGTGCTCGATCTTGGCGCGGCTCCCGGGTCATGGTCGCTTG
GCGCGGCGGAAAAAATCGGACCTTCGGGCAGGGTGCTGGCTGCCGACATCCAGACCACAGAGACTGTTTTTCCGCCCAAT
GTCACGTTTATGCAGGAAGATGTCTTTGAGCGTTCTGCAGAATTCGAGCAGGCGCTGTCCGAGACCGGTCCTTTTGACGT
CGTCATAAGTGATATGGCTCCTAAAACCACGGGCCACAAGTTCACCGATCAGGCCAGATCGTCCAATCTGTGCTTTGAAG
CGCTTGCGGTTGCCTCACTTCATCTCGTAGAAGGCGGCAGCTTTGTGGTCAAGATTTTCATGGGACCGGATGTAGAAGCG
TACGTGAAGCAGATGCGTACGCTGTTTACCGCTGTTAAGTCGTTCAAGCCCAAAAGCTCGCGCTCCGAGAGTAAGGAAAC
CTTTTATATCGGACTGGGGTTTAAAGGGCTGCCGGAGTTTTCTGAACAAGAGGGCGAAGACTGA

Upstream 100 bases:

>100_bases
ACGGTAATGCGGGGCGGACGAAAGCTGGTTGTGCGGCGTACAGTCATGAGCGGCCTTGGCGTTCGGGGCACTTTCGGATA
CAAGTGCCGCAGGTGAAGCA

Downstream 100 bases:

>100_bases
AAGGTCTTTTTCGCAGAACAGTTTTGCAATGACCGGAGCGCGCTGCGGCGCGCTGACTGGGGGCTGCCGTTGTGCCTGAC
ACGGCGGGGTGCTCCGGTGG

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKYRDHYFLKAKRENYPARSVYKLKEIDKRFGILKQGMRVLDLGAAPGSWSLGAAEKIGPSGRVLAADIQTTETVFPPN
VTFMQEDVFERSAEFEQALSETGPFDVVISDMAPKTTGHKFTDQARSSNLCFEALAVASLHLVEGGSFVVKIFMGPDVEA
YVKQMRTLFTAVKSFKPKSSRSESKETFYIGLGFKGLPEFSEQEGED

Sequences:

>Translated_207_residues
MKKYRDHYFLKAKRENYPARSVYKLKEIDKRFGILKQGMRVLDLGAAPGSWSLGAAEKIGPSGRVLAADIQTTETVFPPN
VTFMQEDVFERSAEFEQALSETGPFDVVISDMAPKTTGHKFTDQARSSNLCFEALAVASLHLVEGGSFVVKIFMGPDVEA
YVKQMRTLFTAVKSFKPKSSRSESKETFYIGLGFKGLPEFSEQEGED
>Mature_207_residues
MKKYRDHYFLKAKRENYPARSVYKLKEIDKRFGILKQGMRVLDLGAAPGSWSLGAAEKIGPSGRVLAADIQTTETVFPPN
VTFMQEDVFERSAEFEQALSETGPFDVVISDMAPKTTGHKFTDQARSSNLCFEALAVASLHLVEGGSFVVKIFMGPDVEA
YVKQMRTLFTAVKSFKPKSSRSESKETFYIGLGFKGLPEFSEQEGED

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=38.7755102040816, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=38.7755102040816, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=38.7755102040816, Blast_Score=123, Evalue=1e-28,
Organism=Homo sapiens, GI7019377, Length=208, Percent_Identity=35.0961538461538, Blast_Score=113, Evalue=9e-26,
Organism=Homo sapiens, GI194097365, Length=194, Percent_Identity=33.5051546391753, Blast_Score=107, Evalue=8e-24,
Organism=Escherichia coli, GI1789569, Length=194, Percent_Identity=37.6288659793814, Blast_Score=124, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17553474, Length=196, Percent_Identity=32.6530612244898, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17554650, Length=194, Percent_Identity=34.5360824742268, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=191, Percent_Identity=31.413612565445, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71987561, Length=191, Percent_Identity=31.413612565445, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17553860, Length=191, Percent_Identity=31.413612565445, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319535, Length=206, Percent_Identity=35.4368932038835, Blast_Score=107, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6319796, Length=193, Percent_Identity=32.6424870466321, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321302, Length=132, Percent_Identity=37.8787878787879, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356387, Length=205, Percent_Identity=35.609756097561, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647580, Length=210, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648639, Length=212, Percent_Identity=33.9622641509434, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI18859957, Length=200, Percent_Identity=30, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_DESDG (Q30YX3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388818.1
- ProteinModelPortal:   Q30YX3
- SMR:   Q30YX3
- STRING:   Q30YX3
- GeneID:   3757337
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2326
- NMPDR:   fig|207559.3.peg.620
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK705668
- BioCyc:   DDES207559:DDE_2326-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23085; Mature: 23085

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: ACT_SITE 151-151 BINDING 49-49 BINDING 51-51 BINDING 69-69 BINDING 87-87 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYRDHYFLKAKRENYPARSVYKLKEIDKRFGILKQGMRVLDLGAAPGSWSLGAAEKIG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHCC
PSGRVLAADIQTTETVFPPNVTFMQEDVFERSAEFEQALSETGPFDVVISDMAPKTTGHK
CCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
FTDQARSSNLCFEALAVASLHLVEGGSFVVKIFMGPDVEAYVKQMRTLFTAVKSFKPKSS
HHHHHCCCCHHHHHHHHHHHHEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
RSESKETFYIGLGFKGLPEFSEQEGED
CCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKYRDHYFLKAKRENYPARSVYKLKEIDKRFGILKQGMRVLDLGAAPGSWSLGAAEKIG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCHHHCC
PSGRVLAADIQTTETVFPPNVTFMQEDVFERSAEFEQALSETGPFDVVISDMAPKTTGHK
CCCCEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
FTDQARSSNLCFEALAVASLHLVEGGSFVVKIFMGPDVEAYVKQMRTLFTAVKSFKPKSS
HHHHHCCCCHHHHHHHHHHHHEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
RSESKETFYIGLGFKGLPEFSEQEGED
CCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA