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The map label for this gene is glgB

Identifier: 78357328

GI number: 78357328

Start: 2307165

End: 2309081

Strand: Reverse

Name: glgB

Synonym: Dde_2285

Alternate gene names: 78357328

Gene position: 2309081-2307165 (Counterclockwise)

Preceding gene: 78357329

Following gene: 78357327

Centisome position: 61.9

GC content: 56.91

Gene sequence:

>1917_bases
GTGGCAACACCTGTCGAGTCCGGAACCGTGCCGTGCAGTCTGGAACCGTTTGACGTGTATCTTTTCGGTCGCGGAGAGCA
TTGGGATATATACAGAGTGCTGGGCGCGCATGCCCATGCTCAGGACGGTGAAACCGGATACCGGTTTGCGGTATGGGCGC
CCAATGCGCATGCGGTAAGTCTGGTTGGGCCGTTCAATGACTGGCGTTCCGGCGATTTTCCTTTGTTTCCTGTCGGAACA
TCCGGCATCTGGGCCGGATTTGTCGCCGGCATGGAATACGGGCGGCTGTACAAGTTTGCCGTACAGGGTGCCGACGGCAC
TGTGCGTTTGAAAACAGACCCCTATGCCTTGTATTGCGAAATGCGGCCCGGCACGGCGTCATTTACTTGGTCGCTTGACA
GCTATGCGTGGAACGACGCTGCATGGATGCAGCGTAGACGTGAAGCCGGTCCGCCTTTGCAGCAGCCGGTCAGCATATAT
GAACTCCATGCCGGTTCGTGGATGCGCCGCCATGGTGAAGGGCATCCTTTTGTAAACTGGGATGAACTGGCCGGCACACT
GATACCCTATGTCCGCGACGCAGGCTTTACTCATATCGAGCTTATGCCCGTGGCTGAGCATCCGCTGGATCAGTCGTGGG
GATACCAGACCGGCGCCTACTATGCGCCCACTTCGCGCTTTGGAACGCCCGATGATTTGAGACGGTTTGTAGACCTTTGC
CACCAGCAGGGCATAGGGGTGATTCTGGACTGGGTACCCGCGCATTTTCCCAAGGATGACTGGAGTCTGGGGCGGTTTGA
CGGCACAGCCCTGTACGAGCATCTGGACCCGCGTCTCGGCGAGCATCCGGACTGGGGTACCTATGTGTTCAATTACGGAC
GGCATGAGGTGCGCAACTTTCTGTTTGCCAACGCGCTCTACTGGTTCAAGGAATTTCATGTGGACGGTTTGCGCATAGAT
GCAGTGGCATCCATGCTGTATCTTGACTATTCCCGCAAGGAAGGGGAGTGGCTGCCCAATGTGCACGGCGGCAATGAAAA
TCTGGAAGCCATTGATTTTCTGCGCGAACTGAACAGAGTGGTGCACGAGCAGTACCCCGGTGTGATGATGATAGCGGAAG
AATCCACCTCATGGCCGGGGGTGTCGCGGCCGTTGTACACGGGGGGGCTGGGGTTCACGTTTAAATGGAACATGGGCTGG
ATGCACGATACGCTGAATTATATGCGGCAGGACCCTGTGTTCAGGGCATACCAGCACAGCAGCCTGACATTTTCCATGCT
GTATGCTTTTTCCGAAAACTTTGTCCTGCCGCTTTCGCATGATGAAGTTGTGCACGGCAAAGGGGCGCTGCTTTCTAAAA
TGCCCGGCGACATGTGGCAGCAGCAGGCCAATTTGCGGTTGATGTACGCATACATGTGGGCGCATCCGGGTAAAAAACTG
CTGTTTATGGGCGGTGAGATAGGTCAGTGGAACGAGTGGAGCGAAAGCCGTGAACCGGACTGGTGCCTGCGTGAATTTCC
TGCCCACGAAGGCATACGCAATCTGGTGCGCGACCTTAACGGCATATATGCACAGGAACCGGCCATGCACCGCCACGACC
ACGACTGGTCGGGGTTCCGCTGGCTCGATTTCAGCGATTACGGATGTTCCGTCATCAGTTTTGCCCGTTTTGCGGAAGAT
AGTCCGCCTGTGATGTGGGTGTTCAATTTTACGCCGGTAGTCCGTCGCTGGTACCGTGTTCCATGCCCCAGAGCCGGAGA
ATGGCAGGAAGTGCTGAATACGGACAGCGGTTACTACGGCGGCAGCAACGTGGGCAACGGGGGCGGTGCGGTAGCCTGCA
CCGACAACTGGCACGGCGGGCATTTTATGGAGCTGACTCTGCCTCCGCTGGCGGCCGTGGCGTTAAAACCTGTGTGA

Upstream 100 bases:

>100_bases
GTCAGAAGAGCCATGGAAGTTTCATTCACATGGGAAAAAGCCGCAGTGCAGTATCTGGACATTTATCAGCAGCTGGGGTT
TGTCCCCGCGGGAGAATGAC

Downstream 100 bases:

>100_bases
TTCTGCCGGTAAAGGAATGCAGTCAGGGCATGGCCGGATACGACCGGCGCAGAGGCTTTCGGTTGTCAGTGCCGGAGCCT
CGGCTCCTGCAGTCAGGCAG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MATPVESGTVPCSLEPFDVYLFGRGEHWDIYRVLGAHAHAQDGETGYRFAVWAPNAHAVSLVGPFNDWRSGDFPLFPVGT
SGIWAGFVAGMEYGRLYKFAVQGADGTVRLKTDPYALYCEMRPGTASFTWSLDSYAWNDAAWMQRRREAGPPLQQPVSIY
ELHAGSWMRRHGEGHPFVNWDELAGTLIPYVRDAGFTHIELMPVAEHPLDQSWGYQTGAYYAPTSRFGTPDDLRRFVDLC
HQQGIGVILDWVPAHFPKDDWSLGRFDGTALYEHLDPRLGEHPDWGTYVFNYGRHEVRNFLFANALYWFKEFHVDGLRID
AVASMLYLDYSRKEGEWLPNVHGGNENLEAIDFLRELNRVVHEQYPGVMMIAEESTSWPGVSRPLYTGGLGFTFKWNMGW
MHDTLNYMRQDPVFRAYQHSSLTFSMLYAFSENFVLPLSHDEVVHGKGALLSKMPGDMWQQQANLRLMYAYMWAHPGKKL
LFMGGEIGQWNEWSESREPDWCLREFPAHEGIRNLVRDLNGIYAQEPAMHRHDHDWSGFRWLDFSDYGCSVISFARFAED
SPPVMWVFNFTPVVRRWYRVPCPRAGEWQEVLNTDSGYYGGSNVGNGGGAVACTDNWHGGHFMELTLPPLAAVALKPV

Sequences:

>Translated_638_residues
MATPVESGTVPCSLEPFDVYLFGRGEHWDIYRVLGAHAHAQDGETGYRFAVWAPNAHAVSLVGPFNDWRSGDFPLFPVGT
SGIWAGFVAGMEYGRLYKFAVQGADGTVRLKTDPYALYCEMRPGTASFTWSLDSYAWNDAAWMQRRREAGPPLQQPVSIY
ELHAGSWMRRHGEGHPFVNWDELAGTLIPYVRDAGFTHIELMPVAEHPLDQSWGYQTGAYYAPTSRFGTPDDLRRFVDLC
HQQGIGVILDWVPAHFPKDDWSLGRFDGTALYEHLDPRLGEHPDWGTYVFNYGRHEVRNFLFANALYWFKEFHVDGLRID
AVASMLYLDYSRKEGEWLPNVHGGNENLEAIDFLRELNRVVHEQYPGVMMIAEESTSWPGVSRPLYTGGLGFTFKWNMGW
MHDTLNYMRQDPVFRAYQHSSLTFSMLYAFSENFVLPLSHDEVVHGKGALLSKMPGDMWQQQANLRLMYAYMWAHPGKKL
LFMGGEIGQWNEWSESREPDWCLREFPAHEGIRNLVRDLNGIYAQEPAMHRHDHDWSGFRWLDFSDYGCSVISFARFAED
SPPVMWVFNFTPVVRRWYRVPCPRAGEWQEVLNTDSGYYGGSNVGNGGGAVACTDNWHGGHFMELTLPPLAAVALKPV
>Mature_637_residues
ATPVESGTVPCSLEPFDVYLFGRGEHWDIYRVLGAHAHAQDGETGYRFAVWAPNAHAVSLVGPFNDWRSGDFPLFPVGTS
GIWAGFVAGMEYGRLYKFAVQGADGTVRLKTDPYALYCEMRPGTASFTWSLDSYAWNDAAWMQRRREAGPPLQQPVSIYE
LHAGSWMRRHGEGHPFVNWDELAGTLIPYVRDAGFTHIELMPVAEHPLDQSWGYQTGAYYAPTSRFGTPDDLRRFVDLCH
QQGIGVILDWVPAHFPKDDWSLGRFDGTALYEHLDPRLGEHPDWGTYVFNYGRHEVRNFLFANALYWFKEFHVDGLRIDA
VASMLYLDYSRKEGEWLPNVHGGNENLEAIDFLRELNRVVHEQYPGVMMIAEESTSWPGVSRPLYTGGLGFTFKWNMGWM
HDTLNYMRQDPVFRAYQHSSLTFSMLYAFSENFVLPLSHDEVVHGKGALLSKMPGDMWQQQANLRLMYAYMWAHPGKKLL
FMGGEIGQWNEWSESREPDWCLREFPAHEGIRNLVRDLNGIYAQEPAMHRHDHDWSGFRWLDFSDYGCSVISFARFAEDS
PPVMWVFNFTPVVRRWYRVPCPRAGEWQEVLNTDSGYYGGSNVGNGGGAVACTDNWHGGHFMELTLPPLAAVALKPV

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=635, Percent_Identity=26.2992125984252, Blast_Score=164, Evalue=4e-40,
Organism=Escherichia coli, GI1789839, Length=625, Percent_Identity=53.12, Blast_Score=649, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=660, Percent_Identity=24.8484848484848, Blast_Score=161, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32564391, Length=527, Percent_Identity=25.2371916508539, Blast_Score=146, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320826, Length=670, Percent_Identity=25.6716417910448, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI28573410, Length=649, Percent_Identity=28.5053929121726, Blast_Score=199, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_DESDG (Q30Z14)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388777.1
- ProteinModelPortal:   Q30Z14
- SMR:   Q30Z14
- STRING:   Q30Z14
- GeneID:   3757296
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2285
- NMPDR:   fig|207559.3.peg.2169
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   PPLATIM
- ProtClustDB:   PRK05402
- BioCyc:   DDES207559:DDE_2285-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat

EC number: =2.4.1.18

Molecular weight: Translated: 72879; Mature: 72747

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: ACT_SITE 215-215 ACT_SITE 250-250 ACT_SITE 255-255 ACT_SITE 318-318 ACT_SITE 320-320 ACT_SITE 373-373 ACT_SITE 440-440 ACT_SITE 441-441

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPVESGTVPCSLEPFDVYLFGRGEHWDIYRVLGAHAHAQDGETGYRFAVWAPNAHAVS
CCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCEEE
LVGPFNDWRSGDFPLFPVGTSGIWAGFVAGMEYGRLYKFAVQGADGTVRLKTDPYALYCE
EEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEEEEEEECCCCEEEEECCCEEEEEE
MRPGTASFTWSLDSYAWNDAAWMQRRREAGPPLQQPVSIYELHAGSWMRRHGEGHPFVNW
ECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCH
DELAGTLIPYVRDAGFTHIELMPVAEHPLDQSWGYQTGAYYAPTSRFGTPDDLRRFVDLC
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
HQQGIGVILDWVPAHFPKDDWSLGRFDGTALYEHLDPRLGEHPDWGTYVFNYGRHEVRNF
HHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHH
LFANALYWFKEFHVDGLRIDAVASMLYLDYSRKEGEWLPNVHGGNENLEAIDFLRELNRV
HHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VHEQYPGVMMIAEESTSWPGVSRPLYTGGLGFTFKWNMGWMHDTLNYMRQDPVFRAYQHS
HHHHCCCEEEEEECCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHCCCHHHHHHCC
SLTFSMLYAFSENFVLPLSHDEVVHGKGALLSKMPGDMWQQQANLRLMYAYMWAHPGKKL
CCCEEEEEHHCCCCEEECCCCCEECCCCCHHHCCCCHHHHHCCCCEEEEEEEECCCCCEE
LFMGGEIGQWNEWSESREPDWCLREFPAHEGIRNLVRDLNGIYAQEPAMHRHDHDWSGFR
EEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
WLDFSDYGCSVISFARFAEDSPPVMWVFNFTPVVRRWYRVPCPRAGEWQEVLNTDSGYYG
EEECCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
GSNVGNGGGAVACTDNWHGGHFMELTLPPLAAVALKPV
CCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHEECCC
>Mature Secondary Structure 
ATPVESGTVPCSLEPFDVYLFGRGEHWDIYRVLGAHAHAQDGETGYRFAVWAPNAHAVS
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCEEE
LVGPFNDWRSGDFPLFPVGTSGIWAGFVAGMEYGRLYKFAVQGADGTVRLKTDPYALYCE
EEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEEEEEEECCCCEEEEECCCEEEEEE
MRPGTASFTWSLDSYAWNDAAWMQRRREAGPPLQQPVSIYELHAGSWMRRHGEGHPFVNW
ECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHCCCCCCCCCH
DELAGTLIPYVRDAGFTHIELMPVAEHPLDQSWGYQTGAYYAPTSRFGTPDDLRRFVDLC
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHH
HQQGIGVILDWVPAHFPKDDWSLGRFDGTALYEHLDPRLGEHPDWGTYVFNYGRHEVRNF
HHCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHH
LFANALYWFKEFHVDGLRIDAVASMLYLDYSRKEGEWLPNVHGGNENLEAIDFLRELNRV
HHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
VHEQYPGVMMIAEESTSWPGVSRPLYTGGLGFTFKWNMGWMHDTLNYMRQDPVFRAYQHS
HHHHCCCEEEEEECCCCCCCCCCCEEECCCCEEEEECCCHHHHHHHHHHCCCHHHHHHCC
SLTFSMLYAFSENFVLPLSHDEVVHGKGALLSKMPGDMWQQQANLRLMYAYMWAHPGKKL
CCCEEEEEHHCCCCEEECCCCCEECCCCCHHHCCCCHHHHHCCCCEEEEEEEECCCCCEE
LFMGGEIGQWNEWSESREPDWCLREFPAHEGIRNLVRDLNGIYAQEPAMHRHDHDWSGFR
EEECCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
WLDFSDYGCSVISFARFAEDSPPVMWVFNFTPVVRRWYRVPCPRAGEWQEVLNTDSGYYG
EEECCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCC
GSNVGNGGGAVACTDNWHGGHFMELTLPPLAAVALKPV
CCCCCCCCCEEEEECCCCCCEEEEEECCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA