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The map label for this gene is thiH

Identifier: 78357321

GI number: 78357321

Start: 2299179

End: 2300588

Strand: Reverse

Name: thiH

Synonym: Dde_2278

Alternate gene names: 78357321

Gene position: 2300588-2299179 (Counterclockwise)

Preceding gene: 78357322

Following gene: 78357320

Centisome position: 61.67

GC content: 55.6

Gene sequence:

>1410_bases
ATGTCTTTTGATTCCCGTTCCCTGCCAGGCTTCATAGACGAAGAAAAAATCGAATCTGTGATTGCCGCAACGGCCAAACC
TGACGCTGTGCGTGTGCGCGAAATTCTCGCCAAGGCACGTGAAGCAAAGGGCCTTGATGCCGAAGAGACCGCAACCCTGC
TGCAACTCGATAACGAAGAACTGGATGCGGAGCTGTTTGCCACAGCCAAAAAGGTTAAACAGACCATCTACGGTAACCGG
CTTGTTCTTTTTGCTCCTCTTTATATTACCAACGAATGCTATAACCGGTGTGCCTATTGCGGATTTAACGCCACAAACAG
CGATCTGAAGCGCCGGACTCTCTCGGAAGATGAAATCCGGGCCGAAGTGGAAGTGCTGGAACGTCTGGGGCATAAGCGCC
TGCTGCTTGTGTACGGAGAGCACCCGCGCCTTGATGCCGACTGGATGGCACGCACCATTCAGGTGGTGTATGATACTGTT
TCTGAAAAAAGCGGTGAAATCCGCCGTGTGAACATCAACTGTGCCCCGCAGACCGTGGACGGCTTCAGAAAGCTGCACGA
TGTGGGCATAGGTACCTACCAGTGTTTTCAGGAAACCTACCACAAGGCGACGTATGACAAGGCGCATCTGGGCGGTCCCA
AAAAGGATTACCTGTGGCGGTTGTATGCCATGCACCGCGCCATGGAGGCCGGCATCGACGACGTGGGCATGGGCCCCCTG
CTCGGTCTGTACGACTACCGGTTTGAGATTCTTGCACTGATGCAGCATGCCGCCGATCTGGAAAAACATTTCGGCGTAGG
CCCGCATACCATCTCTTTCCCCAGGCTGGAACCGGCCCTCAATGCCGATATGGCATTCAATCCGCCGCACCCGCTCACCG
ATTCCCAGTTTAAACGAATGGTTGCCGTGCTCCGGCTGGCAGTGCCGTATACAGGGCTTATTCTCAGCACGCGTGAAAAT
GCAGCCATGCGGCGTGAACTGCTCGAGCTGGGCGTTTCGCAGATCAGTGCGGGTTCGCGCACCTATCCGGGTGCCTACAG
CGACCCGAGCTACGACCGGCCCGATGTGCAGCAGTTCTGCGTAGGCGACAGCCGCAGTCTGGACGAGGTCATAGCAGAGC
TTGTCTCTTTGGGATACCTGCCCTCGTGGTGCACGGCCTGTTACCGTCTGGGCCGTACCGGCGAACACTTTATGGAGCTG
GCAAAAAAAGGCTTCATTCAGGAATTCTGCCATCCCAACGCGCTGCTTACCTTCAATGAATATCTGCATGACTACGCTTC
TGAATCGACACGCGAAGCGGGCAGAAAGCTTATTGAAAAAGAGGCGGCAGGCTGTCCGGAAAACAGGCGCGAGCTTGTTG
CTTCGCGTCTGCAGCGCATAGACGGCGGCGAGCGCGATTTGTACATCTGA

Upstream 100 bases:

>100_bases
GTCGAAGCCTCCACGGACGAGATAGGTTCGTTGACGGGCAGGCTGGGACGGCTGGAAAGTGTAACGGTGAAAGCGTCTCT
AGTCTGATGCCGGAGTACGT

Downstream 100 bases:

>100_bases
TCCGTTCTTTCAGGCATATACAGCGGCCGGAAGCTCCGGCTTCCGGCCGCTTAACCATTACGTGCTCTATAGCTGAAATT
TCTCTTTTCCCGGGCGGGTT

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSFDSRSLPGFIDEEKIESVIAATAKPDAVRVREILAKAREAKGLDAEETATLLQLDNEELDAELFATAKKVKQTIYGNR
LVLFAPLYITNECYNRCAYCGFNATNSDLKRRTLSEDEIRAEVEVLERLGHKRLLLVYGEHPRLDADWMARTIQVVYDTV
SEKSGEIRRVNINCAPQTVDGFRKLHDVGIGTYQCFQETYHKATYDKAHLGGPKKDYLWRLYAMHRAMEAGIDDVGMGPL
LGLYDYRFEILALMQHAADLEKHFGVGPHTISFPRLEPALNADMAFNPPHPLTDSQFKRMVAVLRLAVPYTGLILSTREN
AAMRRELLELGVSQISAGSRTYPGAYSDPSYDRPDVQQFCVGDSRSLDEVIAELVSLGYLPSWCTACYRLGRTGEHFMEL
AKKGFIQEFCHPNALLTFNEYLHDYASESTREAGRKLIEKEAAGCPENRRELVASRLQRIDGGERDLYI

Sequences:

>Translated_469_residues
MSFDSRSLPGFIDEEKIESVIAATAKPDAVRVREILAKAREAKGLDAEETATLLQLDNEELDAELFATAKKVKQTIYGNR
LVLFAPLYITNECYNRCAYCGFNATNSDLKRRTLSEDEIRAEVEVLERLGHKRLLLVYGEHPRLDADWMARTIQVVYDTV
SEKSGEIRRVNINCAPQTVDGFRKLHDVGIGTYQCFQETYHKATYDKAHLGGPKKDYLWRLYAMHRAMEAGIDDVGMGPL
LGLYDYRFEILALMQHAADLEKHFGVGPHTISFPRLEPALNADMAFNPPHPLTDSQFKRMVAVLRLAVPYTGLILSTREN
AAMRRELLELGVSQISAGSRTYPGAYSDPSYDRPDVQQFCVGDSRSLDEVIAELVSLGYLPSWCTACYRLGRTGEHFMEL
AKKGFIQEFCHPNALLTFNEYLHDYASESTREAGRKLIEKEAAGCPENRRELVASRLQRIDGGERDLYI
>Mature_468_residues
SFDSRSLPGFIDEEKIESVIAATAKPDAVRVREILAKAREAKGLDAEETATLLQLDNEELDAELFATAKKVKQTIYGNRL
VLFAPLYITNECYNRCAYCGFNATNSDLKRRTLSEDEIRAEVEVLERLGHKRLLLVYGEHPRLDADWMARTIQVVYDTVS
EKSGEIRRVNINCAPQTVDGFRKLHDVGIGTYQCFQETYHKATYDKAHLGGPKKDYLWRLYAMHRAMEAGIDDVGMGPLL
GLYDYRFEILALMQHAADLEKHFGVGPHTISFPRLEPALNADMAFNPPHPLTDSQFKRMVAVLRLAVPYTGLILSTRENA
AMRRELLELGVSQISAGSRTYPGAYSDPSYDRPDVQQFCVGDSRSLDEVIAELVSLGYLPSWCTACYRLGRTGEHFMELA
KKGFIQEFCHPNALLTFNEYLHDYASESTREAGRKLIEKEAAGCPENRRELVASRLQRIDGGERDLYI

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=336, Percent_Identity=32.7380952380952, Blast_Score=194, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53008; Mature: 52877

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDSRSLPGFIDEEKIESVIAATAKPDAVRVREILAKAREAKGLDAEETATLLQLDNEE
CCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
LDAELFATAKKVKQTIYGNRLVLFAPLYITNECYNRCAYCGFNATNSDLKRRTLSEDEIR
HHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHH
AEVEVLERLGHKRLLLVYGEHPRLDADWMARTIQVVYDTVSEKSGEIRRVNINCAPQTVD
HHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
GFRKLHDVGIGTYQCFQETYHKATYDKAHLGGPKKDYLWRLYAMHRAMEAGIDDVGMGPL
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
LGLYDYRFEILALMQHAADLEKHFGVGPHTISFPRLEPALNADMAFNPPHPLTDSQFKRM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VAVLRLAVPYTGLILSTRENAAMRRELLELGVSQISAGSRTYPGAYSDPSYDRPDVQQFC
HHHHHHHCCHHCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
VGDSRSLDEVIAELVSLGYLPSWCTACYRLGRTGEHFMELAKKGFIQEFCHPNALLTFNE
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEHHHH
YLHDYASESTREAGRKLIEKEAAGCPENRRELVASRLQRIDGGERDLYI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SFDSRSLPGFIDEEKIESVIAATAKPDAVRVREILAKAREAKGLDAEETATLLQLDNEE
CCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHH
LDAELFATAKKVKQTIYGNRLVLFAPLYITNECYNRCAYCGFNATNSDLKRRTLSEDEIR
HHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHH
AEVEVLERLGHKRLLLVYGEHPRLDADWMARTIQVVYDTVSEKSGEIRRVNINCAPQTVD
HHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
GFRKLHDVGIGTYQCFQETYHKATYDKAHLGGPKKDYLWRLYAMHRAMEAGIDDVGMGPL
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHH
LGLYDYRFEILALMQHAADLEKHFGVGPHTISFPRLEPALNADMAFNPPHPLTDSQFKRM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VAVLRLAVPYTGLILSTRENAAMRRELLELGVSQISAGSRTYPGAYSDPSYDRPDVQQFC
HHHHHHHCCHHCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
VGDSRSLDEVIAELVSLGYLPSWCTACYRLGRTGEHFMELAKKGFIQEFCHPNALLTFNE
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEHHHH
YLHDYASESTREAGRKLIEKEAAGCPENRRELVASRLQRIDGGERDLYI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]