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The map label for this gene is bioB [H]

Identifier: 78357320

GI number: 78357320

Start: 2298065

End: 2299078

Strand: Reverse

Name: bioB [H]

Synonym: Dde_2277

Alternate gene names: 78357320

Gene position: 2299078-2298065 (Counterclockwise)

Preceding gene: 78357321

Following gene: 78357319

Centisome position: 61.63

GC content: 59.76

Gene sequence:

>1014_bases
ATGACATCCAGAGATATTTTGGAAATGCTGGCTGCCACCGGCGGCGGCCCCGTGTACGCAGAGGCGCGCACCGTTGCTGA
CAGGTTTTTCGGGCGCGGCGTATATGTCCGCGGTGTTGTCGAGTTTTCAAACCATTGCAGAAAAAACTGCCATTACTGCG
GACTGCGCGTTGCCAACACAGGGCTTGAACGTTTCCGGCTGGAGCCGGAAGGCATTCTGGCGGCAGCGGCATTGGCGCGC
GAGCTGGGGGCGGGTACCGTGGTGCTCCAGTCGGGCGAAGACCTGCGCTACGACAGGCGGGTTATCGGCGATCTTGTCCG
GCGCATCAGGGATACGCTGGATGTGGCGGTGACTCTTTCGCTGGGGGATTTTGACCGGGACACATATGCCTACTGGCGTG
ACTGCGGTGCGGACAGGTATCTTCTCAAAATGGAAACTTTTGATGAAGCTCTGCATGCGCGTCTGCGCCCCGGCTGTACG
GTTGCTGACCGGCTTGCCCGCGTGGAGATGCTGCAGTCGTTGGGGTATGAAACGGGATCGGGCATCATCGTAGGTCTGCC
GGGTATGACCGACGCCATACTGGCGGAGGATATACACAGACTTTCACAGCTCGGCCTTGAAATGATTGCCGCCGGTCCGT
TCATTCCTCACCCGAGCACCCCTCTGGCTGCCCCTGTGGATCATGCCATTGAAAAAAGCCTGCTGGTGACAGCAGTTCTG
CGGCTTTTGAATCCCGGGGCCAATATTCCCGCCACAAGTGCTCTGGATGCACTGGCAGCCGATGGACGCACCCGCGGGCT
GGATGCCGGGGCCAACGTTGTCATGCCTTCGGTCACTCCCGACGCGGTGCGCGGCGGCTACTCCATATATCCGGGAAAAA
ACGCTGCAGGGCGCGATGTGCGCGATGCCGTGCACGGTCTTTTTGAACGTCTGAGAAATGCCGGGTACACCCCTGTTGCT
GACAAAGGCTTCAGCCGGATTGCCGGGGCATGCGGAGGAAATGCTGATGTCTGA

Upstream 100 bases:

>100_bases
TCCGTTCTTTCAGGCATATACAGCGGCCGGAAGCTCCGGCTTCCGGCCGCTTAACCATTACGTGCTCTATAGCTGAAATT
TCTCTTTTCCCGGGCGGGTT

Downstream 100 bases:

>100_bases
TAAAGCGCCACGCGGCGAACGGCTGGTGATTACTCTTGTGGGACGGCGTAATGCCGGTAAGTCTTCGCTTATCAACGCCC
TTGCCGGTCAGCAGATAGCC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTSRDILEMLAATGGGPVYAEARTVADRFFGRGVYVRGVVEFSNHCRKNCHYCGLRVANTGLERFRLEPEGILAAAALAR
ELGAGTVVLQSGEDLRYDRRVIGDLVRRIRDTLDVAVTLSLGDFDRDTYAYWRDCGADRYLLKMETFDEALHARLRPGCT
VADRLARVEMLQSLGYETGSGIIVGLPGMTDAILAEDIHRLSQLGLEMIAAGPFIPHPSTPLAAPVDHAIEKSLLVTAVL
RLLNPGANIPATSALDALAADGRTRGLDAGANVVMPSVTPDAVRGGYSIYPGKNAAGRDVRDAVHGLFERLRNAGYTPVA
DKGFSRIAGACGGNADV

Sequences:

>Translated_337_residues
MTSRDILEMLAATGGGPVYAEARTVADRFFGRGVYVRGVVEFSNHCRKNCHYCGLRVANTGLERFRLEPEGILAAAALAR
ELGAGTVVLQSGEDLRYDRRVIGDLVRRIRDTLDVAVTLSLGDFDRDTYAYWRDCGADRYLLKMETFDEALHARLRPGCT
VADRLARVEMLQSLGYETGSGIIVGLPGMTDAILAEDIHRLSQLGLEMIAAGPFIPHPSTPLAAPVDHAIEKSLLVTAVL
RLLNPGANIPATSALDALAADGRTRGLDAGANVVMPSVTPDAVRGGYSIYPGKNAAGRDVRDAVHGLFERLRNAGYTPVA
DKGFSRIAGACGGNADV
>Mature_336_residues
TSRDILEMLAATGGGPVYAEARTVADRFFGRGVYVRGVVEFSNHCRKNCHYCGLRVANTGLERFRLEPEGILAAAALARE
LGAGTVVLQSGEDLRYDRRVIGDLVRRIRDTLDVAVTLSLGDFDRDTYAYWRDCGADRYLLKMETFDEALHARLRPGCTV
ADRLARVEMLQSLGYETGSGIIVGLPGMTDAILAEDIHRLSQLGLEMIAAGPFIPHPSTPLAAPVDHAIEKSLLVTAVLR
LLNPGANIPATSALDALAADGRTRGLDAGANVVMPSVTPDAVRGGYSIYPGKNAAGRDVRDAVHGLFERLRNAGYTPVAD
KGFSRIAGACGGNADV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36066; Mature: 35934

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRDILEMLAATGGGPVYAEARTVADRFFGRGVYVRGVVEFSNHCRKNCHYCGLRVANT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHC
GLERFRLEPEGILAAAALARELGAGTVVLQSGEDLRYDRRVIGDLVRRIRDTLDVAVTLS
CHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
LGDFDRDTYAYWRDCGADRYLLKMETFDEALHARLRPGCTVADRLARVEMLQSLGYETGS
ECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
GIIVGLPGMTDAILAEDIHRLSQLGLEMIAAGPFIPHPSTPLAAPVDHAIEKSLLVTAVL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RLLNPGANIPATSALDALAADGRTRGLDAGANVVMPSVTPDAVRGGYSIYPGKNAAGRDV
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHCCCEEECCCCCCCCCHH
RDAVHGLFERLRNAGYTPVADKGFSRIAGACGGNADV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSRDILEMLAATGGGPVYAEARTVADRFFGRGVYVRGVVEFSNHCRKNCHYCGLRVANT
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCHHHHC
GLERFRLEPEGILAAAALARELGAGTVVLQSGEDLRYDRRVIGDLVRRIRDTLDVAVTLS
CHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEE
LGDFDRDTYAYWRDCGADRYLLKMETFDEALHARLRPGCTVADRLARVEMLQSLGYETGS
ECCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
GIIVGLPGMTDAILAEDIHRLSQLGLEMIAAGPFIPHPSTPLAAPVDHAIEKSLLVTAVL
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RLLNPGANIPATSALDALAADGRTRGLDAGANVVMPSVTPDAVRGGYSIYPGKNAAGRDV
HHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHCCCEEECCCCCCCCCHH
RDAVHGLFERLRNAGYTPVADKGFSRIAGACGGNADV
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA