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The map label for this gene is mnmE [H]

Identifier: 78357319

GI number: 78357319

Start: 2296870

End: 2298072

Strand: Reverse

Name: mnmE [H]

Synonym: Dde_2276

Alternate gene names: 78357319

Gene position: 2298072-2296870 (Counterclockwise)

Preceding gene: 78357320

Following gene: 78357310

Centisome position: 61.61

GC content: 56.94

Gene sequence:

>1203_bases
ATGTCTGATAAAGCGCCACGCGGCGAACGGCTGGTGATTACTCTTGTGGGACGGCGTAATGCCGGTAAGTCTTCGCTTAT
CAACGCCCTTGCCGGTCAGCAGATAGCCATAGTTTCCGATGTTCCCGGCACAACGACGGATCCGGTGGCAAAGCCCTATG
AGCTGCTGCCGCTGGGACCGGTTACTTTTTATGACACGGCCGGGCTGGACGATTCCGGTGAACTGGGCGAAATGCGGGTC
CGTTCTGCCAGAAAGGTGCTGGCCCGTACGGATGTCGCGCTGCTGGTTGTCAGTGAAGCCGGTATGGAAGAGGCGGAAAA
GCGGATGCTGGCCGATCTGCAGGCAATGGAAATTTCCGCGCTGGTTGTTTTTAACAAACAGGATATAGCAGATGTGCGGC
CCGAAGATGTTCGTTTTTGCCACGAGGCAGGAGTCCGCCATGTACAGGTTTCTTCCGTGGCGCAAAAGGGTATCAGCGAG
CTGAAGTCGGCCATTGTCGAAATGGTGCCCGAAGAGCTGAAAGCGGATCCAGTGCTTGTTTCCGATCTTATTTCCGAAGG
TGATACAGTTCTCTGTGTAGTCCCCATTGATCTGGCTGCCCCGAAGGGGCGTCTGATCCTGCCGCAGGTGCAGGTGCTGC
GCGATGTTCTGGACGCGGACGCCATGGGGATGGTGGTGAAGGAGCGAGAACTGGAAGCTGCTCTGGACAAGCTTGTATCG
CCGCCGGCGCTGGTGATCACTGATTCGCAGGTGGTGCTCAAAGTGGCCGGTGACGTTGACGATGACATTCCCATGACCAC
TTTTTCCACATTGTTTGCCCGCTATAAGGGTGACCTTGAGCTGCTTGTCCGAGGTGCCCGCGCCATCGACTCACTGCGCG
ACGGCGACACAGTGCTGATGTGCGAAGCCTGTTCGCACCATGCCGTTGCCGATGATATCGGCAGGGTGAAGATACCCCGC
TGGATAACACAGTACACCGGCAGAGAGCTTTCATTCGAAATGTATGCGGGGCATGATTTCCCCGAAGATCTTGAAAGATA
CGCACTGGCTGTGCACTGCGGCGGCTGCATGACCAACCGCGCCGAAATGATGCGGCGCATACGCGAATGTACACGCAGAG
GGGTGCCCGTCACAAATTACGGCGTGGCCATTTCAAAGGTGCAGGGCGTTCTGGAGCGGGTCGTCGCTCCTTTCGGGTTG
TAA

Upstream 100 bases:

>100_bases
GCCGTGCACGGTCTTTTTGAACGTCTGAGAAATGCCGGGTACACCCCTGTTGCTGACAAAGGCTTCAGCCGGATTGCCGG
GGCATGCGGAGGAAATGCTG

Downstream 100 bases:

>100_bases
CGGGGCTGTTTCGGCTTTTCCGGCGGAGCCTGTCCGTAATGCCGTTGCAGGGCTGATTTGTGTTGCCGCCGGTGCAGCGG
TGTCCCGTCAGCATCCGGCC

Product: small GTP-binding protein domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSDKAPRGERLVITLVGRRNAGKSSLINALAGQQIAIVSDVPGTTTDPVAKPYELLPLGPVTFYDTAGLDDSGELGEMRV
RSARKVLARTDVALLVVSEAGMEEAEKRMLADLQAMEISALVVFNKQDIADVRPEDVRFCHEAGVRHVQVSSVAQKGISE
LKSAIVEMVPEELKADPVLVSDLISEGDTVLCVVPIDLAAPKGRLILPQVQVLRDVLDADAMGMVVKERELEAALDKLVS
PPALVITDSQVVLKVAGDVDDDIPMTTFSTLFARYKGDLELLVRGARAIDSLRDGDTVLMCEACSHHAVADDIGRVKIPR
WITQYTGRELSFEMYAGHDFPEDLERYALAVHCGGCMTNRAEMMRRIRECTRRGVPVTNYGVAISKVQGVLERVVAPFGL

Sequences:

>Translated_400_residues
MSDKAPRGERLVITLVGRRNAGKSSLINALAGQQIAIVSDVPGTTTDPVAKPYELLPLGPVTFYDTAGLDDSGELGEMRV
RSARKVLARTDVALLVVSEAGMEEAEKRMLADLQAMEISALVVFNKQDIADVRPEDVRFCHEAGVRHVQVSSVAQKGISE
LKSAIVEMVPEELKADPVLVSDLISEGDTVLCVVPIDLAAPKGRLILPQVQVLRDVLDADAMGMVVKERELEAALDKLVS
PPALVITDSQVVLKVAGDVDDDIPMTTFSTLFARYKGDLELLVRGARAIDSLRDGDTVLMCEACSHHAVADDIGRVKIPR
WITQYTGRELSFEMYAGHDFPEDLERYALAVHCGGCMTNRAEMMRRIRECTRRGVPVTNYGVAISKVQGVLERVVAPFGL
>Mature_399_residues
SDKAPRGERLVITLVGRRNAGKSSLINALAGQQIAIVSDVPGTTTDPVAKPYELLPLGPVTFYDTAGLDDSGELGEMRVR
SARKVLARTDVALLVVSEAGMEEAEKRMLADLQAMEISALVVFNKQDIADVRPEDVRFCHEAGVRHVQVSSVAQKGISEL
KSAIVEMVPEELKADPVLVSDLISEGDTVLCVVPIDLAAPKGRLILPQVQVLRDVLDADAMGMVVKERELEAALDKLVSP
PALVITDSQVVLKVAGDVDDDIPMTTFSTLFARYKGDLELLVRGARAIDSLRDGDTVLMCEACSHHAVADDIGRVKIPRW
ITQYTGRELSFEMYAGHDFPEDLERYALAVHCGGCMTNRAEMMRRIRECTRRGVPVTNYGVAISKVQGVLERVVAPFGL

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG1160

COG function: function code R; Predicted GTPases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 43502; Mature: 43371

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKAPRGERLVITLVGRRNAGKSSLINALAGQQIAIVSDVPGTTTDPVAKPYELLPLGP
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEECCC
VTFYDTAGLDDSGELGEMRVRSARKVLARTDVALLVVSEAGMEEAEKRMLADLQAMEISA
EEEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHEEEE
LVVFNKQDIADVRPEDVRFCHEAGVRHVQVSSVAQKGISELKSAIVEMVPEELKADPVLV
EEEECCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHH
SDLISEGDTVLCVVPIDLAAPKGRLILPQVQVLRDVLDADAMGMVVKERELEAALDKLVS
HHHHCCCCEEEEEEEEECCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PPALVITDSQVVLKVAGDVDDDIPMTTFSTLFARYKGDLELLVRGARAIDSLRDGDTVLM
CCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
CEACSHHAVADDIGRVKIPRWITQYTGRELSFEMYAGHDFPEDLERYALAVHCGGCMTNR
EECCCCCCHHHHCCCEECCHHHHHHCCCCEEEEEECCCCCHHHHHHHEEEEECCCCCCCH
AEMMRRIRECTRRGVPVTNYGVAISKVQGVLERVVAPFGL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDKAPRGERLVITLVGRRNAGKSSLINALAGQQIAIVSDVPGTTTDPVAKPYELLPLGP
CCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEEECCC
VTFYDTAGLDDSGELGEMRVRSARKVLARTDVALLVVSEAGMEEAEKRMLADLQAMEISA
EEEEECCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHEEEE
LVVFNKQDIADVRPEDVRFCHEAGVRHVQVSSVAQKGISELKSAIVEMVPEELKADPVLV
EEEECCCCCCCCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCHHHHCCCCHHH
SDLISEGDTVLCVVPIDLAAPKGRLILPQVQVLRDVLDADAMGMVVKERELEAALDKLVS
HHHHCCCCEEEEEEEEECCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
PPALVITDSQVVLKVAGDVDDDIPMTTFSTLFARYKGDLELLVRGARAIDSLRDGDTVLM
CCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEE
CEACSHHAVADDIGRVKIPRWITQYTGRELSFEMYAGHDFPEDLERYALAVHCGGCMTNR
EECCCCCCHHHHCCCEECCHHHHHHCCCCEEEEEECCCCCHHHHHHHEEEEECCCCCCCH
AEMMRRIRECTRRGVPVTNYGVAISKVQGVLERVVAPFGL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA