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The map label for this gene is cysL [H]

Identifier: 78357269

GI number: 78357269

Start: 2266322

End: 2267281

Strand: Reverse

Name: cysL [H]

Synonym: Dde_2226

Alternate gene names: 78357269

Gene position: 2267281-2266322 (Counterclockwise)

Preceding gene: 78357270

Following gene: 78357268

Centisome position: 60.78

GC content: 53.12

Gene sequence:

>960_bases
ATGTGTGTAAAGCCCAATCGGCCCAATGCTCTTGCGTTATCCGTCTTGCCGATGTACTGCATAGACATGATTGATTTCAG
AAGGCTAGAAGCATTTTGCAAGGTGTATGAGTTGCGCAGCTTTTCACGAGCCGGCGATGAGCTTTTTTTGTCTCAGCCCA
CCATCAGCGCACATGTTTCTGCGCTGGAAAAGGAACTGGATGTCAGGCTGCTGGACAGAATGGGCCGCGGTGTTTTGCCC
ACGCCTGCCGGAGAAATCCTGTACCGGCATGCAATGACCGCGTTTGCCAGCCTTGAAACTGCTCATCTGGAGATAAGCCA
GTTGCAGGATATCATTACCGGTGAAGTTCCGGTCGGTGCCAGCACCATTCCAGCCCAGTACCTGTTGCCACCGGTTATGG
CCGCTTATATGCGTTCGTTTCCTGAGGTTCAGTTACAACTGCGTACAGGAGATACCGGCGAGATAATCCGTAAAGTCGGG
GCAGGTGAGCTTACGCTCGGTGTGGTAGGTGCTCATGACCAGCAGCCGGATATCGTGTTTACTCCCATTATCGATGATGA
GCTTATCATTATCGGCGCACCGGATTTTGCTCCCCGCAGCGGTAGTGTTTCTTGCGAATCGCTGGCGGATATGCCCTGGG
TCGTGCGCGAAGCAGGTTCCGGAACATGGAAGATTTTTTCCGCAGCGTTGGGAACGCATGGCATCGATTGCCGGAGTTTT
CGTCCAGTGGCCTGTGTTGACAGCTCGCAGTCTGTCATTCAATGTGTCCGCGCCGGACTGGGGGTTAGTGTCACCTCCCG
CATCGCTGTTGCGGAATTTCTTTCACGCGGCGAACTGGTTGAAATTACAGTGGACGGACTGGATGTCCGCCGCGAGTTTT
TCTGTATTTATCACTCACGGCGGCAGCTGTTTCCGGCGGCACGCCATTTTATCGAGTTTTTGACAAAAAAATGCCGTTGA

Upstream 100 bases:

>100_bases
CCGTGCGGAATATTTGTCCGCGGGGAGATAGCCTGCTTCTGCTCTCGCATATCCGGCTTGCAGGTGTGCGCGCGGCCGGT
ACTTTTTTTGTGCGCCAGTA

Downstream 100 bases:

>100_bases
ACACGACAGGCTGAAGGAAGCTCCAATGTCCCGCATCACGCTGGTTGATAAAGTAAAAGCTGCAGGCTGAGCAGCAAAGA
TAGCTCCGGGGGACCTGGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MCVKPNRPNALALSVLPMYCIDMIDFRRLEAFCKVYELRSFSRAGDELFLSQPTISAHVSALEKELDVRLLDRMGRGVLP
TPAGEILYRHAMTAFASLETAHLEISQLQDIITGEVPVGASTIPAQYLLPPVMAAYMRSFPEVQLQLRTGDTGEIIRKVG
AGELTLGVVGAHDQQPDIVFTPIIDDELIIIGAPDFAPRSGSVSCESLADMPWVVREAGSGTWKIFSAALGTHGIDCRSF
RPVACVDSSQSVIQCVRAGLGVSVTSRIAVAEFLSRGELVEITVDGLDVRREFFCIYHSRRQLFPAARHFIEFLTKKCR

Sequences:

>Translated_319_residues
MCVKPNRPNALALSVLPMYCIDMIDFRRLEAFCKVYELRSFSRAGDELFLSQPTISAHVSALEKELDVRLLDRMGRGVLP
TPAGEILYRHAMTAFASLETAHLEISQLQDIITGEVPVGASTIPAQYLLPPVMAAYMRSFPEVQLQLRTGDTGEIIRKVG
AGELTLGVVGAHDQQPDIVFTPIIDDELIIIGAPDFAPRSGSVSCESLADMPWVVREAGSGTWKIFSAALGTHGIDCRSF
RPVACVDSSQSVIQCVRAGLGVSVTSRIAVAEFLSRGELVEITVDGLDVRREFFCIYHSRRQLFPAARHFIEFLTKKCR
>Mature_319_residues
MCVKPNRPNALALSVLPMYCIDMIDFRRLEAFCKVYELRSFSRAGDELFLSQPTISAHVSALEKELDVRLLDRMGRGVLP
TPAGEILYRHAMTAFASLETAHLEISQLQDIITGEVPVGASTIPAQYLLPPVMAAYMRSFPEVQLQLRTGDTGEIIRKVG
AGELTLGVVGAHDQQPDIVFTPIIDDELIIIGAPDFAPRSGSVSCESLADMPWVVREAGSGTWKIFSAALGTHGIDCRSF
RPVACVDSSQSVIQCVRAGLGVSVTSRIAVAEFLSRGELVEITVDGLDVRREFFCIYHSRRQLFPAARHFIEFLTKKCR

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=32.0689655172414, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1788297, Length=189, Percent_Identity=30.1587301587302, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=27.7227722772277, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787879, Length=301, Percent_Identity=27.2425249169435, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787601, Length=232, Percent_Identity=25.8620689655172, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1788748, Length=283, Percent_Identity=25.4416961130742, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790399, Length=194, Percent_Identity=26.8041237113402, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082132, Length=294, Percent_Identity=24.8299319727891, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=167, Percent_Identity=29.3413173652695, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787128, Length=300, Percent_Identity=25, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790208, Length=199, Percent_Identity=27.6381909547739, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787589, Length=184, Percent_Identity=33.1521739130435, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=25.4237288135593, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789639, Length=147, Percent_Identity=30.6122448979592, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=236, Percent_Identity=26.271186440678, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789506, Length=241, Percent_Identity=22.4066390041494, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=287, Percent_Identity=21.602787456446, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081904, Length=229, Percent_Identity=24.4541484716157, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=23.9837398373984, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=172, Percent_Identity=27.3255813953488, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789440, Length=264, Percent_Identity=22.3484848484848, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=22.3107569721116, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35099; Mature: 35099

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVKPNRPNALALSVLPMYCIDMIDFRRLEAFCKVYELRSFSRAGDELFLSQPTISAHVS
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
ALEKELDVRLLDRMGRGVLPTPAGEILYRHAMTAFASLETAHLEISQLQDIITGEVPVGA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
STIPAQYLLPPVMAAYMRSFPEVQLQLRTGDTGEIIRKVGAGELTLGVVGAHDQQPDIVF
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCEEE
TPIIDDELIIIGAPDFAPRSGSVSCESLADMPWVVREAGSGTWKIFSAALGTHGIDCRSF
EEECCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCC
RPVACVDSSQSVIQCVRAGLGVSVTSRIAVAEFLSRGELVEITVDGLDVRREFFCIYHSR
CCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHH
RQLFPAARHFIEFLTKKCR
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCVKPNRPNALALSVLPMYCIDMIDFRRLEAFCKVYELRSFSRAGDELFLSQPTISAHVS
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
ALEKELDVRLLDRMGRGVLPTPAGEILYRHAMTAFASLETAHLEISQLQDIITGEVPVGA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
STIPAQYLLPPVMAAYMRSFPEVQLQLRTGDTGEIIRKVGAGELTLGVVGAHDQQPDIVF
CCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCEEEEEEECCCCCCCEEE
TPIIDDELIIIGAPDFAPRSGSVSCESLADMPWVVREAGSGTWKIFSAALGTHGIDCRSF
EEECCCCEEEEECCCCCCCCCCCCHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCCC
RPVACVDSSQSVIQCVRAGLGVSVTSRIAVAEFLSRGELVEITVDGLDVRREFFCIYHSR
CCEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHH
RQLFPAARHFIEFLTKKCR
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]