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The map label for this gene is tig

Identifier: 78357265

GI number: 78357265

Start: 2262649

End: 2263965

Strand: Reverse

Name: tig

Synonym: Dde_2222

Alternate gene names: 78357265

Gene position: 2263965-2262649 (Counterclockwise)

Preceding gene: 78357268

Following gene: 78357263

Centisome position: 60.69

GC content: 53.45

Gene sequence:

>1317_bases
ATGGAATATAATGTCGAAGACCTTTCGCCGGTTAAAAAGAAAATTACTATCACAGTGCCCGTTGAAGAAGTGAATGCCGC
TCTGGCTGCCACCATCGCCGTGTACCAGACCAGTGTGAACCTTTCCGGTTTCCGCAAGGGTAAAGTGCCTGCCAGCATCA
TCGAAGGACGCTTCCGTAAGGAAGTATACAACGAAGCCACTCAGGATCTGGTGAACGTGCATATCAACGAAGTTGTGGGT
TCGCTCGACACCCAGCCTGCTTCGCGCATCGATTTTGATGGCGGGCAGCTGGAACGTGACAAGGAATTTGTCTACACCAT
TTCTTTTGAAGTGATGCCCGTGTTTGAAATGCCTGATTATGACGGCATGGAAGTTGAGCAGGAAAAGCCCGAGGTTGACG
AAAACGAAGTCAACGCCGTGCTGGAGCGCATTCGTACCAACCTTGCCGACGTGGTTACCGTGGCAGAACACCGTCCCGCC
AAAGACGGCGAGATTGCCGTGCTGGATTTTGCCGCTTATGAAAACGGTGAGCCCATTGCCGGTGTGGCCGCAGATAATTT
TGAACTGAATCTGGGCGGCAATCAGGCGCTGGTCGATTTTGAAGAGCTGGTGAAGCGCGCCGTGCCCGGCGAAGCAACCG
AAGGCGATGTCACCTTCCCCGAAGATTTCATCAACCCCGAGTTTGCAGGCAAGACCGTCACCATGAAAGTGACCGTGCAC
GCCGTGAAAGAACGCAAGCTGCCCGAACTTGATGACGAACTGGCCAAAAAGGCCGGCGGCTTCGAGTCTTTTGATAAAAT
GCGTGAAACCGTGGAACAGTCCTATCTGGAAAGCCGCACTCAGCTGTGCAAGGCTCAGGCCCAGAAGACCATGGTGGACA
GCCTGCTCAAGACGCTGGACTTCCCTGTACCTGAATCCATGCTGGAAATGTACATGGACAGCCTGCTTGCCGAAAAGAAA
GACCGTCTTGAACGTCAGGGCAAAAGCCTTGAGTCTCTGGGCAAGTCTCCTGAAGAGCTGCGCGACGAAGTGCGCCCCGA
GGCAGAGCACATTGCCCGTACCCAGATTTTTCTGGTAAAGGCAGCCCAGAAGGAAGGCGTAACTGTTTCCGAGCAGGAAG
TGGATCGTCAGCTGCAGCAGATTGCCATGCGCAGCGGCCAGGATTTCCAGACCATGAAGGAACACTACACCCGTACCAAC
ATGATTTTTAACCTGCGTGACCGCATGCTGGCTGACAAAGCCATGGAAGCCATCTACGAAAAAGCGGCCATCAAAGAAGT
TCCCGCAAAGAAAGAAGCAGCCGAAGCCGCTGAATAG

Upstream 100 bases:

>100_bases
CCTCAGCGGACCATGCCGTTAAAAATCCCGCCGGGCGTCCGCCGGAAAGCCGGAGACCCTGAGCCAGCACAGACGCCTCT
GGCGTGTAAGGAGCGAACCC

Downstream 100 bases:

>100_bases
TTTCTGCTGTATCTGTTGTTGTTTTGCAGGGGAAGTGCACCGCACTTCCCCTGTTTTTGTCTAAAATGCGGCAGGCGCTA
TTGACTGCGGCCCGTCGCGT

Product: trigger factor

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MEYNVEDLSPVKKKITITVPVEEVNAALAATIAVYQTSVNLSGFRKGKVPASIIEGRFRKEVYNEATQDLVNVHINEVVG
SLDTQPASRIDFDGGQLERDKEFVYTISFEVMPVFEMPDYDGMEVEQEKPEVDENEVNAVLERIRTNLADVVTVAEHRPA
KDGEIAVLDFAAYENGEPIAGVAADNFELNLGGNQALVDFEELVKRAVPGEATEGDVTFPEDFINPEFAGKTVTMKVTVH
AVKERKLPELDDELAKKAGGFESFDKMRETVEQSYLESRTQLCKAQAQKTMVDSLLKTLDFPVPESMLEMYMDSLLAEKK
DRLERQGKSLESLGKSPEELRDEVRPEAEHIARTQIFLVKAAQKEGVTVSEQEVDRQLQQIAMRSGQDFQTMKEHYTRTN
MIFNLRDRMLADKAMEAIYEKAAIKEVPAKKEAAEAAE

Sequences:

>Translated_438_residues
MEYNVEDLSPVKKKITITVPVEEVNAALAATIAVYQTSVNLSGFRKGKVPASIIEGRFRKEVYNEATQDLVNVHINEVVG
SLDTQPASRIDFDGGQLERDKEFVYTISFEVMPVFEMPDYDGMEVEQEKPEVDENEVNAVLERIRTNLADVVTVAEHRPA
KDGEIAVLDFAAYENGEPIAGVAADNFELNLGGNQALVDFEELVKRAVPGEATEGDVTFPEDFINPEFAGKTVTMKVTVH
AVKERKLPELDDELAKKAGGFESFDKMRETVEQSYLESRTQLCKAQAQKTMVDSLLKTLDFPVPESMLEMYMDSLLAEKK
DRLERQGKSLESLGKSPEELRDEVRPEAEHIARTQIFLVKAAQKEGVTVSEQEVDRQLQQIAMRSGQDFQTMKEHYTRTN
MIFNLRDRMLADKAMEAIYEKAAIKEVPAKKEAAEAAE
>Mature_438_residues
MEYNVEDLSPVKKKITITVPVEEVNAALAATIAVYQTSVNLSGFRKGKVPASIIEGRFRKEVYNEATQDLVNVHINEVVG
SLDTQPASRIDFDGGQLERDKEFVYTISFEVMPVFEMPDYDGMEVEQEKPEVDENEVNAVLERIRTNLADVVTVAEHRPA
KDGEIAVLDFAAYENGEPIAGVAADNFELNLGGNQALVDFEELVKRAVPGEATEGDVTFPEDFINPEFAGKTVTMKVTVH
AVKERKLPELDDELAKKAGGFESFDKMRETVEQSYLESRTQLCKAQAQKTMVDSLLKTLDFPVPESMLEMYMDSLLAEKK
DRLERQGKSLESLGKSPEELRDEVRPEAEHIARTQIFLVKAAQKEGVTVSEQEVDRQLQQIAMRSGQDFQTMKEHYTRTN
MIFNLRDRMLADKAMEAIYEKAAIKEVPAKKEAAEAAE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=429, Percent_Identity=25.6410256410256, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_DESDG (Q30Z77)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388714.1
- ProteinModelPortal:   Q30Z77
- SMR:   Q30Z77
- STRING:   Q30Z77
- GeneID:   3757232
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2222
- NMPDR:   fig|207559.3.peg.2184
- eggNOG:   COG0544
- HOGENOM:   HBG458144
- OMA:   RFIPGFE
- ProtClustDB:   CLSK705168
- BioCyc:   DDES207559:DDE_2222-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 49153; Mature: 49153

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYNVEDLSPVKKKITITVPVEEVNAALAATIAVYQTSVNLSGFRKGKVPASIIEGRFRK
CCCCCHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
EVYNEATQDLVNVHINEVVGSLDTQPASRIDFDGGQLERDKEFVYTISFEVMPVFEMPDY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCC
DGMEVEQEKPEVDENEVNAVLERIRTNLADVVTVAEHRPAKDGEIAVLDFAAYENGEPIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCE
GVAADNFELNLGGNQALVDFEELVKRAVPGEATEGDVTFPEDFINPEFAGKTVTMKVTVH
EEEECCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEE
AVKERKLPELDDELAKKAGGFESFDKMRETVEQSYLESRTQLCKAQAQKTMVDSLLKTLD
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPVPESMLEMYMDSLLAEKKDRLERQGKSLESLGKSPEELRDEVRPEAEHIARTQIFLVK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHEEEE
AAQKEGVTVSEQEVDRQLQQIAMRSGQDFQTMKEHYTRTNMIFNLRDRMLADKAMEAIYE
ECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAIKEVPAKKEAAEAAE
HHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MEYNVEDLSPVKKKITITVPVEEVNAALAATIAVYQTSVNLSGFRKGKVPASIIEGRFRK
CCCCCHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
EVYNEATQDLVNVHINEVVGSLDTQPASRIDFDGGQLERDKEFVYTISFEVMPVFEMPDY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEECCCC
DGMEVEQEKPEVDENEVNAVLERIRTNLADVVTVAEHRPAKDGEIAVLDFAAYENGEPIA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCE
GVAADNFELNLGGNQALVDFEELVKRAVPGEATEGDVTFPEDFINPEFAGKTVTMKVTVH
EEEECCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEEE
AVKERKLPELDDELAKKAGGFESFDKMRETVEQSYLESRTQLCKAQAQKTMVDSLLKTLD
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPVPESMLEMYMDSLLAEKKDRLERQGKSLESLGKSPEELRDEVRPEAEHIARTQIFLVK
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHCCHHHHHHHHHHEEEE
AAQKEGVTVSEQEVDRQLQQIAMRSGQDFQTMKEHYTRTNMIFNLRDRMLADKAMEAIYE
ECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAAIKEVPAKKEAAEAAE
HHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA