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The map label for this gene is yfkO [H]

Identifier: 78357242

GI number: 78357242

Start: 2239295

End: 2239954

Strand: Direct

Name: yfkO [H]

Synonym: Dde_2199

Alternate gene names: 78357242

Gene position: 2239295-2239954 (Clockwise)

Preceding gene: 78357241

Following gene: 78357251

Centisome position: 60.03

GC content: 55.76

Gene sequence:

>660_bases
ATGAATGAGACAGTCCTTACCCCTTTCCTGTTCCGGCATGCCAGCAAGGATTTCGATCCCGACAGAAAAATTCCCGAAGA
CCAGTTTCTGACCATTCTGGAAGCAGGCAGACTCGCGCCCAGTTCCTTCGGCTTTGAACCGTGGAAGTTTCTGATAGTGC
AGAACCCCGCCCTGCGCGCCGAGCTGCACCGGCACACATGGGGCGGCAAAAAGCAGATTCCCAACTGCAGCCATCTGGTT
GTCTATCTGGCCCACAAACGCCCGCGTCTGCTGCCGGAAAGCGATTATATACAGTCTTCCATGCGTGAGGTGCTGCAACT
GCCCGACGACATCATAGCCCTGAAAACGGACTATTACGGCAACTTCCTGCGCAGCGATTTCGGCATGATAGATAACGAGA
TGCGGCTTTTTGAATGGTCGTGCAGGCAGGCGTACATAGCGCTGGCCAACATGATGACAGCGGCAGCCATGATGGAAATC
GACAGCTGCGCACTGGAAGGCTTTGTTGAAGCGGACCTGAACACCGCCGTGAAGCAGCACCTTCAGGTGGATACGGATCA
GTTCGGTGTTGCCTGCATGTGTGCCTTTGGCTACAGAGTGCGCCCCCCGCGGCCCAAAGCCCGCCACAGCATGGAAAACG
TGGTACGCTGGTACGATTAA

Upstream 100 bases:

>100_bases
GGCGGCAATGCATTCTGTTTTTACAGATATGCCTTTACAAGACGCGACCTGTGGCTAGAGTAACAGTCTGGCCCAATAGC
AAGCAGATACGGAGAACGCC

Downstream 100 bases:

>100_bases
TCTGCCGCAAAGACAACGGACAATGACGGGGCAGGGAACCTCTGCCGCTCCGGTACCGCATGCGGCTGTCCTGCACCGTA
ATCCGCATGCCCGCAAAGAC

Product: nitroreductase family protein

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNETVLTPFLFRHASKDFDPDRKIPEDQFLTILEAGRLAPSSFGFEPWKFLIVQNPALRAELHRHTWGGKKQIPNCSHLV
VYLAHKRPRLLPESDYIQSSMREVLQLPDDIIALKTDYYGNFLRSDFGMIDNEMRLFEWSCRQAYIALANMMTAAAMMEI
DSCALEGFVEADLNTAVKQHLQVDTDQFGVACMCAFGYRVRPPRPKARHSMENVVRWYD

Sequences:

>Translated_219_residues
MNETVLTPFLFRHASKDFDPDRKIPEDQFLTILEAGRLAPSSFGFEPWKFLIVQNPALRAELHRHTWGGKKQIPNCSHLV
VYLAHKRPRLLPESDYIQSSMREVLQLPDDIIALKTDYYGNFLRSDFGMIDNEMRLFEWSCRQAYIALANMMTAAAMMEI
DSCALEGFVEADLNTAVKQHLQVDTDQFGVACMCAFGYRVRPPRPKARHSMENVVRWYD
>Mature_219_residues
MNETVLTPFLFRHASKDFDPDRKIPEDQFLTILEAGRLAPSSFGFEPWKFLIVQNPALRAELHRHTWGGKKQIPNCSHLV
VYLAHKRPRLLPESDYIQSSMREVLQLPDDIIALKTDYYGNFLRSDFGMIDNEMRLFEWSCRQAYIALANMMTAAAMMEI
DSCALEGFVEADLNTAVKQHLQVDTDQFGVACMCAFGYRVRPPRPKARHSMENVVRWYD

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 25320; Mature: 25320

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNETVLTPFLFRHASKDFDPDRKIPEDQFLTILEAGRLAPSSFGFEPWKFLIVQNPALRA
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHH
ELHRHTWGGKKQIPNCSHLVVYLAHKRPRLLPESDYIQSSMREVLQLPDDIIALKTDYYG
HHHHHCCCCCCCCCCHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHEEEEHHHHH
NFLRSDFGMIDNEMRLFEWSCRQAYIALANMMTAAAMMEIDSCALEGFVEADLNTAVKQH
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVDTDQFGVACMCAFGYRVRPPRPKARHSMENVVRWYD
HCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNETVLTPFLFRHASKDFDPDRKIPEDQFLTILEAGRLAPSSFGFEPWKFLIVQNPALRA
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHH
ELHRHTWGGKKQIPNCSHLVVYLAHKRPRLLPESDYIQSSMREVLQLPDDIIALKTDYYG
HHHHHCCCCCCCCCCHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHEEEEHHHHH
NFLRSDFGMIDNEMRLFEWSCRQAYIALANMMTAAAMMEIDSCALEGFVEADLNTAVKQH
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVDTDQFGVACMCAFGYRVRPPRPKARHSMENVVRWYD
HCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]