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The map label for this gene is ilvB [H]

Identifier: 78357211

GI number: 78357211

Start: 2210765

End: 2212453

Strand: Reverse

Name: ilvB [H]

Synonym: Dde_2168

Alternate gene names: 78357211

Gene position: 2212453-2210765 (Counterclockwise)

Preceding gene: 78357212

Following gene: 78357210

Centisome position: 59.31

GC content: 56.96

Gene sequence:

>1689_bases
ATGGAGTCGAGCGGGGCCCGGATCCTGTTGGAGTGTCTTAAAAAGGAAGGCGTTGATGTCATTTTCGGCTACCCCGGCGG
GGCCGTGATCGACATTTACGACGAACTTCCCCGGCATGACCTGCGTCATGTGCTGGTGCGCCACGAACAGGCCGCAGTAC
ATGCCGCGGACGGTTATGCCCGTGCTTCCGGTCGAGTGGGGGTCTGTCTGGTCACTTCGGGACCCGGCGCCACCAATACC
GTGACCGGAATCGCTACAGCGTACTGCGACTCCATTCCTCTGGTGGTGCTAACCGGACAGGTGCCGCGTGCGCTGATAGG
CAATGACGCTTTTCAGGAAGTGGATATAGTGGGCATTACGCGTCCCTGCACCAAGCATAATTATCTGGTGAAGGAACTGA
GCGACTTGCCGCGTATTATCCGCGAAGCTTTTTATCTTGCCCGTTCCGGCAGGCCGGGTCCCGTTCTTGTCGACCTGCCA
AAAGATATCATGGTAGCCCGTGCAGAGTTTCAGATTCCTGAAGAAGTCAAGCTGCGCAGCTACAACCCGACATACAGACC
GAATCTTAACCAGCTGCGCAGAGCGGCGGAACTGGTTCTTTCTGCCAGACGGCCGCTGCTGTTCGCCGGTGGCGGGGTTG
TGGGGTCCGATGCCAGCGAAGAGCTTGGCTGGCTGGCCCGCACGTTGCAGATTCCGGTAACTTCAAGTCTGATGGGGCTT
GGTTCTTTTCCCGGTGACGACCCGCTCTTTCTGGGCATGCTGGGTATGCACGGCACCTTTGCCGCCAACAAGGCGGTGAA
TAGCTGTGACGTGCTTATTGCCGCCGGTGCGCGCTTTGACGACAGAGTGACAGGAAAGCTTTCGGCTTTTGCCGCCGGTG
CCAAGATTATCCACATAGACATCGACCCCACATCCATCAGAAAAAATGTGGGCGTAGAGGTGCCTGTGGTGGGCGACTGC
CGTATGGCGCTTGCTGGCATGCGCGAGATTCTTTCGGCCCGCTTTGAAGAGAACATTCCCGTACCCCGCTGGACTGAGTG
GCTTGCCACGGTGCACGATTGGGCGCAGAGTCATCCTCTGGGGTATGTGCCTTCTGAAGGTATTAAACCGCAGCAGGTTG
TGGAAACTGTTTTCGGTATTACCAGAGGCGAGTGTATTGTCACCACCGAGGTGGGGCAGAACCAGATGTGGGCCGCACAG
TTTTTCAAATACCGTAAACCGCGCACGCTGCTGACTTCCGGCGGACTAGGCACCATGGGATACGGTTTTCCGGCGGCTAT
AGGTGCGCAGATGGCGTTTCCGGGGCATCTGGTGATTGATTTTGCGGGCGACGGTTCCATTCAGATGAATATTCAGGAAC
TGGCCACAGCGGTTTGTAACAAGCTGCCGGTTAAAATAATTATTCTGAACAACGGCTATCTGGGTATGGTGCGCCAGTGG
CAGGAGTTGTTCTATCAGCGTAATTACTGTTCAACCTGCATGGAGGCCCAGCCTGATTTTGTGAAACTGGCTGAAGCCTA
CGGAGCGGAAGGGTACCGCATCACAGCACAGGCTGATCTTGAGCCGGTGTTGCGGCAGGCCCTGCAAAGCCCGGCTCCGG
CTATTGTTGATGTGCGCGTGGAGCGCGAGGAAAATGTCTATCCCATGGTTCCGGCCGGTGCGGCGCTGGACGAGATGCTG
CTTGTGTAG

Upstream 100 bases:

>100_bases
CAGTGAACAGACACATGTGAAAGAACTTAAAAAACAGAAGCTTGAAGGGAAGACAAAGCTTCTGACCATCCTTGAAAGCT
ACCGCAGTAAGGAGAACTAG

Downstream 100 bases:

>100_bases
GAGACTTCCATGCGTCATGTACTTTCCGTTCTGGTGGAAAACGAACCGGGCGTTCTTTCCCGGGTGGCCGGGCTGTTCAG
CGGCAGGGGGTTCAACATTG

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MESSGARILLECLKKEGVDVIFGYPGGAVIDIYDELPRHDLRHVLVRHEQAAVHAADGYARASGRVGVCLVTSGPGATNT
VTGIATAYCDSIPLVVLTGQVPRALIGNDAFQEVDIVGITRPCTKHNYLVKELSDLPRIIREAFYLARSGRPGPVLVDLP
KDIMVARAEFQIPEEVKLRSYNPTYRPNLNQLRRAAELVLSARRPLLFAGGGVVGSDASEELGWLARTLQIPVTSSLMGL
GSFPGDDPLFLGMLGMHGTFAANKAVNSCDVLIAAGARFDDRVTGKLSAFAAGAKIIHIDIDPTSIRKNVGVEVPVVGDC
RMALAGMREILSARFEENIPVPRWTEWLATVHDWAQSHPLGYVPSEGIKPQQVVETVFGITRGECIVTTEVGQNQMWAAQ
FFKYRKPRTLLTSGGLGTMGYGFPAAIGAQMAFPGHLVIDFAGDGSIQMNIQELATAVCNKLPVKIIILNNGYLGMVRQW
QELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITAQADLEPVLRQALQSPAPAIVDVRVEREENVYPMVPAGAALDEML
LV

Sequences:

>Translated_562_residues
MESSGARILLECLKKEGVDVIFGYPGGAVIDIYDELPRHDLRHVLVRHEQAAVHAADGYARASGRVGVCLVTSGPGATNT
VTGIATAYCDSIPLVVLTGQVPRALIGNDAFQEVDIVGITRPCTKHNYLVKELSDLPRIIREAFYLARSGRPGPVLVDLP
KDIMVARAEFQIPEEVKLRSYNPTYRPNLNQLRRAAELVLSARRPLLFAGGGVVGSDASEELGWLARTLQIPVTSSLMGL
GSFPGDDPLFLGMLGMHGTFAANKAVNSCDVLIAAGARFDDRVTGKLSAFAAGAKIIHIDIDPTSIRKNVGVEVPVVGDC
RMALAGMREILSARFEENIPVPRWTEWLATVHDWAQSHPLGYVPSEGIKPQQVVETVFGITRGECIVTTEVGQNQMWAAQ
FFKYRKPRTLLTSGGLGTMGYGFPAAIGAQMAFPGHLVIDFAGDGSIQMNIQELATAVCNKLPVKIIILNNGYLGMVRQW
QELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITAQADLEPVLRQALQSPAPAIVDVRVEREENVYPMVPAGAALDEML
LV
>Mature_562_residues
MESSGARILLECLKKEGVDVIFGYPGGAVIDIYDELPRHDLRHVLVRHEQAAVHAADGYARASGRVGVCLVTSGPGATNT
VTGIATAYCDSIPLVVLTGQVPRALIGNDAFQEVDIVGITRPCTKHNYLVKELSDLPRIIREAFYLARSGRPGPVLVDLP
KDIMVARAEFQIPEEVKLRSYNPTYRPNLNQLRRAAELVLSARRPLLFAGGGVVGSDASEELGWLARTLQIPVTSSLMGL
GSFPGDDPLFLGMLGMHGTFAANKAVNSCDVLIAAGARFDDRVTGKLSAFAAGAKIIHIDIDPTSIRKNVGVEVPVVGDC
RMALAGMREILSARFEENIPVPRWTEWLATVHDWAQSHPLGYVPSEGIKPQQVVETVFGITRGECIVTTEVGQNQMWAAQ
FFKYRKPRTLLTSGGLGTMGYGFPAAIGAQMAFPGHLVIDFAGDGSIQMNIQELATAVCNKLPVKIIILNNGYLGMVRQW
QELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITAQADLEPVLRQALQSPAPAIVDVRVEREENVYPMVPAGAALDEML
LV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=556, Percent_Identity=25.3597122302158, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=26.2068965517241, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=50.7964601769912, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=43.2142857142857, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1786717, Length=574, Percent_Identity=36.0627177700348, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=28.1867145421903, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=28.1639928698752, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17531301, Length=574, Percent_Identity=26.8292682926829, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=574, Percent_Identity=26.8292682926829, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=567, Percent_Identity=26.2786596119929, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=581, Percent_Identity=47.3321858864028, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6320816, Length=571, Percent_Identity=22.9422066549912, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=513, Percent_Identity=23.3918128654971, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=529, Percent_Identity=23.062381852552, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=503, Percent_Identity=24.8508946322068, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19922626, Length=575, Percent_Identity=26.2608695652174, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61058; Mature: 61058

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSGARILLECLKKEGVDVIFGYPGGAVIDIYDELPRHDLRHVLVRHEQAAVHAADGYA
CCCCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHCCHHHHHHHHHHHHHHHEEECCCCE
RASGRVGVCLVTSGPGATNTVTGIATAYCDSIPLVVLTGQVPRALIGNDAFQEVDIVGIT
ECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHEEEEEEEC
RPCTKHNYLVKELSDLPRIIREAFYLARSGRPGPVLVDLPKDIMVARAEFQIPEEVKLRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHEEHHHCCCCHHHEEEC
YNPTYRPNLNQLRRAAELVLSARRPLLFAGGGVVGSDASEELGWLARTLQIPVTSSLMGL
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHHHHHEECCCHHHHHHC
GSFPGDDPLFLGMLGMHGTFAANKAVNSCDVLIAAGARFDDRVTGKLSAFAAGAKIIHID
CCCCCCCCEEEEECCCCCCHHHHCCCCCCCEEEEECCCCCCCHHCHHHHHCCCCEEEEEE
IDPTSIRKNVGVEVPVVGDCRMALAGMREILSARFEENIPVPRWTEWLATVHDWAQSHPL
CCHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
GYVPSEGIKPQQVVETVFGITRGECIVTTEVGQNQMWAAQFFKYRKPRTLLTSGGLGTMG
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCC
YGFPAAIGAQMAFPGHLVIDFAGDGSIQMNIQELATAVCNKLPVKIIILNNGYLGMVRQW
CCCHHHHCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
QELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITAQADLEPVLRQALQSPAPAIVDVRV
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEEEEE
EREENVYPMVPAGAALDEMLLV
ECCCCCCEECCCCCHHHHHHCC
>Mature Secondary Structure
MESSGARILLECLKKEGVDVIFGYPGGAVIDIYDELPRHDLRHVLVRHEQAAVHAADGYA
CCCCCHHHHHHHHHHCCCEEEEECCCCCEEEHHHHCCHHHHHHHHHHHHHHHEEECCCCE
RASGRVGVCLVTSGPGATNTVTGIATAYCDSIPLVVLTGQVPRALIGNDAFQEVDIVGIT
ECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCHHEEEEEEEC
RPCTKHNYLVKELSDLPRIIREAFYLARSGRPGPVLVDLPKDIMVARAEFQIPEEVKLRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHEEHHHCCCCHHHEEEC
YNPTYRPNLNQLRRAAELVLSARRPLLFAGGGVVGSDASEELGWLARTLQIPVTSSLMGL
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCCHHHHHHHHHHEECCCHHHHHHC
GSFPGDDPLFLGMLGMHGTFAANKAVNSCDVLIAAGARFDDRVTGKLSAFAAGAKIIHID
CCCCCCCCEEEEECCCCCCHHHHCCCCCCCEEEEECCCCCCCHHCHHHHHCCCCEEEEEE
IDPTSIRKNVGVEVPVVGDCRMALAGMREILSARFEENIPVPRWTEWLATVHDWAQSHPL
CCHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
GYVPSEGIKPQQVVETVFGITRGECIVTTEVGQNQMWAAQFFKYRKPRTLLTSGGLGTMG
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCC
YGFPAAIGAQMAFPGHLVIDFAGDGSIQMNIQELATAVCNKLPVKIIILNNGYLGMVRQW
CCCHHHHCCCCCCCCEEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
QELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITAQADLEPVLRQALQSPAPAIVDVRV
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCEEEEEEE
EREENVYPMVPAGAALDEMLLV
ECCCCCCEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]