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The map label for this gene is ligA

Identifier: 78357136

GI number: 78357136

Start: 2141016

End: 2143133

Strand: Reverse

Name: ligA

Synonym: Dde_2093

Alternate gene names: 78357136

Gene position: 2143133-2141016 (Counterclockwise)

Preceding gene: 78357137

Following gene: 78357135

Centisome position: 57.45

GC content: 58.03

Gene sequence:

>2118_bases
GTGGACAGACGAGGAGCACCTGTGAAGCAGGATTCGGCCAAGGTTGAACAGCGCATGGAAGAACTGCGCACTTTGCTGGA
GTATCACGGTCACCGGTACTACGTGATGGATGAGCCCGAAATATCCGATGCGGAGTACGATGCCCTTTTCAGGGAACTGG
TGCGGCTGGAGGAGGAACACCCTGAGCATATCCATCCGGGGTCGCCCACGCATAAGGTTGGCGGGCAGATACTAGACGGA
CTGAAGCCCCGTGAGCACTCCCTGCGCATGTACAGTCTGGATAACGCATTCGGTATTGAAGAGTTCCGCGAATTTGTCGA
GCGCGTTGTCCGGCTGGAACCTGATGCTCCGCTTGAATTCTGGGTTGACCCCAAAATGGACGGGTTGGCCATGGAACTTG
TTTATGAAAACGGAGTGTTTTTGCTGGCTGTGACCCGCGGAGACGGCAGCATGGGGGAAGATGTGACCCACACCATGCGC
ACGGTACGCAATGTCCGCATGCGGCTGAATCCGGAAATTGAAGCACCCGTGCGGCTTGAAGTGCGTGGAGAGGTTATTAT
CACCCGCGCCGATTTTGAAGCGCTTAATGCGCGGCAGCAGCAGAAGGGGGGCAAACTGTTCGCCAACCCCCGCAATGCCG
CCGCAGGCTCTGTTCGCCAGCTGGATTCTTCCGTGGCGGCGGCGCGCCCTCTGCGTTTCATGGCATATGGCGTGGGGCAG
GTTGTCTGGGCGGACGGCAGACAGCGCTGGCGGACCCAATATGACATAATGCGGGGACTGCAGGAGCTTGGGTTTGCCGT
ACCTTCTCAGGGCAGACTGTGCGCTGCGCCTGCAGATGTGGAAGCCGCTTTTACCGAGCTTTCTGCCGCGCGGCATGAAC
TGCCGTTTGAAATAGACGGCGTTGTCGCCAAGCTGAACGACCTTGACCTGCAGGAGGCTCTGGGGTTTACGGCCAGGGCT
CCTCGCTGGGCCATTGCGCTTAAATTTCCCGCGCATCAGGCGCGTACCCGCCTTAAGGACATCAGAATTCAGGTGGGGCG
GACTGGTGTGATGACGCCTGTGGCCGAGCTTGAGCCGGTGACTGTGGGCGGGGTGACTGTTTCCAGCGCCACTTTGCATA
ATGAAGATGAAATACGTGCCAAGGGCCTTATGCTGGGCGATGTTGTCATTGTGCAGCGGGCGGGGGATGTGATTCCCGAA
GTGGTACGCGCGGTGCCGGAAGAACGCACCGGAGCTGAACGTGAGTATGTTTTTCCTGCCGTGTGTCCTGTCTGCGGCAG
TGCTGCCGTGCGCGGCGAAGGGGAGGCCGCCTGGCGCTGCACCAATATGCAGTGCCCTGCCGTGCGCAAGCAGGCAATCA
TTCATTTTGTTTCCAAGGCCGGTCTGGACATAGACGGAGTGGGTCGCAAGTGGATTGAGCAGCTGGTGGACAGCGGCAGG
GTGGTTTCTCCGGCTGATCTTTTCCGCATTACGCGGGAGGACCTGCTGGGCATGGAGCGCATGGGCGAAAAACTGGCTTC
CAATTTTATAGAGGCTTTTGATTCGGCACGCCACGAATCCACGCTCCAGCGTTTCATCTGCGCGCTGGGTATCCGCCATG
TGGGTGAGCAGACTGCCAGAACGCTTGCCGCCCGTTTCGGCAATATGGACGAACTGATGCGTGCCGATCAGGAGACATTG
CAGTCTCTGCGTGATATCGGCGGCGAGGTGGCCGGTTCCATACGAGCTTTTTTTGCCAACGGGCAGAACAGGGAGCTTTT
GCAGCAGTTCAAGAATCTTGGTCTGTGGCCCGAGGAGCAGCAGCCTGCAGGTGACAGCACGCCGGTGACACCGCTTGCGG
GCAAAAAAATTCTTTTTACCGGTTCGCTGGTGCGTATGACGCGCTCTGAAGCCAAAGCTATGGCAGAAAAAGCGGGGGCA
GCCGTCATGAGCGGCGTTTCCGCCAGACTTGATATACTTGTGGCCGGAGACAAGCCCGGCAGCAAGCTGGAAAAAGCCCG
TTCACTGGGAATAACGGTTCTGACGGAAGATGAATTCATCAGGCAGGCATCAGAGTCTGAAGAAATTTCCGGACAGGCTG
CAGATGACTATGAAAACAGTCTGCTGCGCGTGCAGTAG

Upstream 100 bases:

>100_bases
TGAGCGGTTTTTTAGAGACGGCGGCGCGGGCGGAAAATGAATACCTTACCGCCCGGCTTGGCCCGCAATACAACGAGCTG
CTTCAGGATATGAAGCGGCA

Downstream 100 bases:

>100_bases
GCGCCAGAGCGAGGAGGACGTCATGAGTGTTTCCGTTATTGTGATGGGTGCCGGCGGGCGTATGGGCACAACCATTACCG
GTATGGTGCGTGCTGACGGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MDRRGAPVKQDSAKVEQRMEELRTLLEYHGHRYYVMDEPEISDAEYDALFRELVRLEEEHPEHIHPGSPTHKVGGQILDG
LKPREHSLRMYSLDNAFGIEEFREFVERVVRLEPDAPLEFWVDPKMDGLAMELVYENGVFLLAVTRGDGSMGEDVTHTMR
TVRNVRMRLNPEIEAPVRLEVRGEVIITRADFEALNARQQQKGGKLFANPRNAAAGSVRQLDSSVAAARPLRFMAYGVGQ
VVWADGRQRWRTQYDIMRGLQELGFAVPSQGRLCAAPADVEAAFTELSAARHELPFEIDGVVAKLNDLDLQEALGFTARA
PRWAIALKFPAHQARTRLKDIRIQVGRTGVMTPVAELEPVTVGGVTVSSATLHNEDEIRAKGLMLGDVVIVQRAGDVIPE
VVRAVPEERTGAEREYVFPAVCPVCGSAAVRGEGEAAWRCTNMQCPAVRKQAIIHFVSKAGLDIDGVGRKWIEQLVDSGR
VVSPADLFRITREDLLGMERMGEKLASNFIEAFDSARHESTLQRFICALGIRHVGEQTARTLAARFGNMDELMRADQETL
QSLRDIGGEVAGSIRAFFANGQNRELLQQFKNLGLWPEEQQPAGDSTPVTPLAGKKILFTGSLVRMTRSEAKAMAEKAGA
AVMSGVSARLDILVAGDKPGSKLEKARSLGITVLTEDEFIRQASESEEISGQAADDYENSLLRVQ

Sequences:

>Translated_705_residues
MDRRGAPVKQDSAKVEQRMEELRTLLEYHGHRYYVMDEPEISDAEYDALFRELVRLEEEHPEHIHPGSPTHKVGGQILDG
LKPREHSLRMYSLDNAFGIEEFREFVERVVRLEPDAPLEFWVDPKMDGLAMELVYENGVFLLAVTRGDGSMGEDVTHTMR
TVRNVRMRLNPEIEAPVRLEVRGEVIITRADFEALNARQQQKGGKLFANPRNAAAGSVRQLDSSVAAARPLRFMAYGVGQ
VVWADGRQRWRTQYDIMRGLQELGFAVPSQGRLCAAPADVEAAFTELSAARHELPFEIDGVVAKLNDLDLQEALGFTARA
PRWAIALKFPAHQARTRLKDIRIQVGRTGVMTPVAELEPVTVGGVTVSSATLHNEDEIRAKGLMLGDVVIVQRAGDVIPE
VVRAVPEERTGAEREYVFPAVCPVCGSAAVRGEGEAAWRCTNMQCPAVRKQAIIHFVSKAGLDIDGVGRKWIEQLVDSGR
VVSPADLFRITREDLLGMERMGEKLASNFIEAFDSARHESTLQRFICALGIRHVGEQTARTLAARFGNMDELMRADQETL
QSLRDIGGEVAGSIRAFFANGQNRELLQQFKNLGLWPEEQQPAGDSTPVTPLAGKKILFTGSLVRMTRSEAKAMAEKAGA
AVMSGVSARLDILVAGDKPGSKLEKARSLGITVLTEDEFIRQASESEEISGQAADDYENSLLRVQ
>Mature_705_residues
MDRRGAPVKQDSAKVEQRMEELRTLLEYHGHRYYVMDEPEISDAEYDALFRELVRLEEEHPEHIHPGSPTHKVGGQILDG
LKPREHSLRMYSLDNAFGIEEFREFVERVVRLEPDAPLEFWVDPKMDGLAMELVYENGVFLLAVTRGDGSMGEDVTHTMR
TVRNVRMRLNPEIEAPVRLEVRGEVIITRADFEALNARQQQKGGKLFANPRNAAAGSVRQLDSSVAAARPLRFMAYGVGQ
VVWADGRQRWRTQYDIMRGLQELGFAVPSQGRLCAAPADVEAAFTELSAARHELPFEIDGVVAKLNDLDLQEALGFTARA
PRWAIALKFPAHQARTRLKDIRIQVGRTGVMTPVAELEPVTVGGVTVSSATLHNEDEIRAKGLMLGDVVIVQRAGDVIPE
VVRAVPEERTGAEREYVFPAVCPVCGSAAVRGEGEAAWRCTNMQCPAVRKQAIIHFVSKAGLDIDGVGRKWIEQLVDSGR
VVSPADLFRITREDLLGMERMGEKLASNFIEAFDSARHESTLQRFICALGIRHVGEQTARTLAARFGNMDELMRADQETL
QSLRDIGGEVAGSIRAFFANGQNRELLQQFKNLGLWPEEQQPAGDSTPVTPLAGKKILFTGSLVRMTRSEAKAMAEKAGA
AVMSGVSARLDILVAGDKPGSKLEKARSLGITVLTEDEFIRQASESEEISGQAADDYENSLLRVQ

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=48.0597014925373, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI87082305, Length=603, Percent_Identity=24.0464344941957, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_DESDG (Q30ZK6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388585.1
- ProteinModelPortal:   Q30ZK6
- STRING:   Q30ZK6
- GeneID:   3757101
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2093
- NMPDR:   fig|207559.3.peg.2241
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK705331
- BioCyc:   DDES207559:DDE_2093-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 78053; Mature: 78053

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 125-125 BINDING 123-123 BINDING 146-146 BINDING 184-184 BINDING 304-304 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRGAPVKQDSAKVEQRMEELRTLLEYHGHRYYVMDEPEISDAEYDALFRELVRLEEEH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PEHIHPGSPTHKVGGQILDGLKPREHSLRMYSLDNAFGIEEFREFVERVVRLEPDAPLEF
CCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEE
WVDPKMDGLAMELVYENGVFLLAVTRGDGSMGEDVTHTMRTVRNVRMRLNPEIEAPVRLE
EECCCCCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
VRGEVIITRADFEALNARQQQKGGKLFANPRNAAAGSVRQLDSSVAAARPLRFMAYGVGQ
EECCEEEEECCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
VVWADGRQRWRTQYDIMRGLQELGFAVPSQGRLCAAPADVEAAFTELSAARHELPFEIDG
EEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEECH
VVAKLNDLDLQEALGFTARAPRWAIALKFPAHQARTRLKDIRIQVGRTGVMTPVAELEPV
HHHHHCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCHHHCCCE
TVGGVTVSSATLHNEDEIRAKGLMLGDVVIVQRAGDVIPEVVRAVPEERTGAEREYVFPA
EECCEEEECCCCCCCHHHHHCCEEEHHEEEEHHHHHHHHHHHHHCCCCCCCCCCCEECHH
VCPVCGSAAVRGEGEAAWRCTNMQCPAVRKQAIIHFVSKAGLDIDGVGRKWIEQLVDSGR
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
VVSPADLFRITREDLLGMERMGEKLASNFIEAFDSARHESTLQRFICALGIRHVGEQTAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAARFGNMDELMRADQETLQSLRDIGGEVAGSIRAFFANGQNRELLQQFKNLGLWPEEQ
HHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
QPAGDSTPVTPLAGKKILFTGSLVRMTRSEAKAMAEKAGAAVMSGVSARLDILVAGDKPG
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
SKLEKARSLGITVLTEDEFIRQASESEEISGQAADDYENSLLRVQ
HHHHHHHHCCCEEEECHHHHHHCCCHHHCCCCCCCCHHCCCEECC
>Mature Secondary Structure
MDRRGAPVKQDSAKVEQRMEELRTLLEYHGHRYYVMDEPEISDAEYDALFRELVRLEEEH
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCC
PEHIHPGSPTHKVGGQILDGLKPREHSLRMYSLDNAFGIEEFREFVERVVRLEPDAPLEF
CCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEE
WVDPKMDGLAMELVYENGVFLLAVTRGDGSMGEDVTHTMRTVRNVRMRLNPEIEAPVRLE
EECCCCCCEEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEE
VRGEVIITRADFEALNARQQQKGGKLFANPRNAAAGSVRQLDSSVAAARPLRFMAYGVGQ
EECCEEEEECCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCC
VVWADGRQRWRTQYDIMRGLQELGFAVPSQGRLCAAPADVEAAFTELSAARHELPFEIDG
EEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCEECH
VVAKLNDLDLQEALGFTARAPRWAIALKFPAHQARTRLKDIRIQVGRTGVMTPVAELEPV
HHHHHCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCCHHHCCCE
TVGGVTVSSATLHNEDEIRAKGLMLGDVVIVQRAGDVIPEVVRAVPEERTGAEREYVFPA
EECCEEEECCCCCCCHHHHHCCEEEHHEEEEHHHHHHHHHHHHHCCCCCCCCCCCEECHH
VCPVCGSAAVRGEGEAAWRCTNMQCPAVRKQAIIHFVSKAGLDIDGVGRKWIEQLVDSGR
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
VVSPADLFRITREDLLGMERMGEKLASNFIEAFDSARHESTLQRFICALGIRHVGEQTAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAARFGNMDELMRADQETLQSLRDIGGEVAGSIRAFFANGQNRELLQQFKNLGLWPEEQ
HHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCC
QPAGDSTPVTPLAGKKILFTGSLVRMTRSEAKAMAEKAGAAVMSGVSARLDILVAGDKPG
CCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
SKLEKARSLGITVLTEDEFIRQASESEEISGQAADDYENSLLRVQ
HHHHHHHHCCCEEEECHHHHHHCCCHHHCCCCCCCCHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA