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The map label for this gene is ksgA

Identifier: 78357102

GI number: 78357102

Start: 2102297

End: 2103139

Strand: Reverse

Name: ksgA

Synonym: Dde_2059

Alternate gene names: 78357102

Gene position: 2103139-2102297 (Counterclockwise)

Preceding gene: 78357103

Following gene: 78357101

Centisome position: 56.38

GC content: 53.5

Gene sequence:

>843_bases
ATGATTGATAAGGAATACCAGCGTATTATGACCGAACGAACCGCATCTGCGCCACGGGCGAAGAAAAGCCTGGGCCAGAA
TTTTCTGCAGGACAAGAATATCTCAGCCAAAATAGTCGCTGCCCTGCAGATCGGCCCGGCAGATTGCGTCATTGAAATCG
GCCCGGGGCCGGGGGCATTGACTGATTTTATCCAGAAGGCTGCGCCCGCAAGTTTGTGGCTGCTGGAAAAAGATACATAC
TGGGCAGGGGAACACCGTCGCTCGGACAGCAGAACGCCGGTGGAAAAACAGGTGGTGCTCACCGATGCCCTGACGTTTCC
GTGGGAACGCCTGTCGGATGACCGTTCATGGAAGCTTATAGGAAATCTGCCCTATAACGTGGCTTCGCCCCTTATGTGGG
ACTGTCTCAGTCTTGCCGCTTTCAGCCGTGCTGTTTTTATGATTCAGAAGGAAGTCGGGGACCGCATTGTGGCCGCCCCG
CGTTCGCGGCAGTACGGCGCGCTCAGTGTATGGTTACAGAGTCATACGGTTCCCAGAAAGGAACTGATCGTGCCGCCTAC
TGTTTTCAAACCCCGGCCCAAGGTTGATTCGGCGGTTCTTTCTTTTGCACCTCTACCGCTTTCGGCCAGAAATTTCAGTC
CGGGGGCTCTTTCAGTTCTTTTAAAGATATGCTTTCAGCAGCGCAGAAAGCAATTGCAAAAAATATTAAAGCGTTACTGG
AGTGATGCAGTGTGTGGCTGGTTTGAGCAGCAGGGGTTGCCGCCCGCCGCCCGGCCTGAGGAATTGTCGCCTAACCAGTT
TCAACAACTTGCAAACCTGCTGGAATCACAGCTTGTGTCTTGA

Upstream 100 bases:

>100_bases
TGATTCGAAAAAATGATATTTTCCGCTGGGTGGATATGTTTCTGCTGGCTGCGTTTTCGCGCCATCTTGATGACTTTCCG
CCGACCTATGCCCGTAAATA

Downstream 100 bases:

>100_bases
CGCAGGGCATTGAAATATGCTTGAGTCTTTTCTGCCGATAGTGCCGGTTGCGCCTCCCAAGGTGTTCTGGTATCGGAAAA
AAAGAAGCTTGAGAAAAAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MIDKEYQRIMTERTASAPRAKKSLGQNFLQDKNISAKIVAALQIGPADCVIEIGPGPGALTDFIQKAAPASLWLLEKDTY
WAGEHRRSDSRTPVEKQVVLTDALTFPWERLSDDRSWKLIGNLPYNVASPLMWDCLSLAAFSRAVFMIQKEVGDRIVAAP
RSRQYGALSVWLQSHTVPRKELIVPPTVFKPRPKVDSAVLSFAPLPLSARNFSPGALSVLLKICFQQRRKQLQKILKRYW
SDAVCGWFEQQGLPPAARPEELSPNQFQQLANLLESQLVS

Sequences:

>Translated_280_residues
MIDKEYQRIMTERTASAPRAKKSLGQNFLQDKNISAKIVAALQIGPADCVIEIGPGPGALTDFIQKAAPASLWLLEKDTY
WAGEHRRSDSRTPVEKQVVLTDALTFPWERLSDDRSWKLIGNLPYNVASPLMWDCLSLAAFSRAVFMIQKEVGDRIVAAP
RSRQYGALSVWLQSHTVPRKELIVPPTVFKPRPKVDSAVLSFAPLPLSARNFSPGALSVLLKICFQQRRKQLQKILKRYW
SDAVCGWFEQQGLPPAARPEELSPNQFQQLANLLESQLVS
>Mature_280_residues
MIDKEYQRIMTERTASAPRAKKSLGQNFLQDKNISAKIVAALQIGPADCVIEIGPGPGALTDFIQKAAPASLWLLEKDTY
WAGEHRRSDSRTPVEKQVVLTDALTFPWERLSDDRSWKLIGNLPYNVASPLMWDCLSLAAFSRAVFMIQKEVGDRIVAAP
RSRQYGALSVWLQSHTVPRKELIVPPTVFKPRPKVDSAVLSFAPLPLSARNFSPGALSVLLKICFQQRRKQLQKILKRYW
SDAVCGWFEQQGLPPAARPEELSPNQFQQLANLLESQLVS

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=284, Percent_Identity=31.3380281690141, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI7657198, Length=196, Percent_Identity=29.0816326530612, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1786236, Length=271, Percent_Identity=35.0553505535055, Blast_Score=138, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25141369, Length=293, Percent_Identity=29.0102389078498, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25146882, Length=220, Percent_Identity=32.2727272727273, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=226, Percent_Identity=29.2035398230088, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357273, Length=286, Percent_Identity=30.4195804195804, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21358017, Length=188, Percent_Identity=30.8510638297872, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_DESDG (Q30ZP0)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388551.1
- ProteinModelPortal:   Q30ZP0
- SMR:   Q30ZP0
- STRING:   Q30ZP0
- GeneID:   3757067
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2059
- NMPDR:   fig|207559.3.peg.2296
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   DDES207559:DDE_2059-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31423; Mature: 31423

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 76-76 BINDING 102-102 BINDING 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKEYQRIMTERTASAPRAKKSLGQNFLQDKNISAKIVAALQIGPADCVIEIGPGPGAL
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCHH
TDFIQKAAPASLWLLEKDTYWAGEHRRSDSRTPVEKQVVLTDALTFPWERLSDDRSWKLI
HHHHHHCCCCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCEEEE
GNLPYNVASPLMWDCLSLAAFSRAVFMIQKEVGDRIVAAPRSRQYGALSVWLQSHTVPRK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHEEEEHHCCCCHH
ELIVPPTVFKPRPKVDSAVLSFAPLPLSARNFSPGALSVLLKICFQQRRKQLQKILKRYW
HEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDAVCGWFEQQGLPPAARPEELSPNQFQQLANLLESQLVS
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDKEYQRIMTERTASAPRAKKSLGQNFLQDKNISAKIVAALQIGPADCVIEIGPGPGAL
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEEEEECCCCCHH
TDFIQKAAPASLWLLEKDTYWAGEHRRSDSRTPVEKQVVLTDALTFPWERLSDDRSWKLI
HHHHHHCCCCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCEEEE
GNLPYNVASPLMWDCLSLAAFSRAVFMIQKEVGDRIVAAPRSRQYGALSVWLQSHTVPRK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHEEEEHHCCCCHH
ELIVPPTVFKPRPKVDSAVLSFAPLPLSARNFSPGALSVLLKICFQQRRKQLQKILKRYW
HEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SDAVCGWFEQQGLPPAARPEELSPNQFQQLANLLESQLVS
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA