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The map label for this gene is hupB [H]

Identifier: 78357101

GI number: 78357101

Start: 2101831

End: 2102103

Strand: Reverse

Name: hupB [H]

Synonym: Dde_2058

Alternate gene names: 78357101

Gene position: 2102103-2101831 (Counterclockwise)

Preceding gene: 78357102

Following gene: 78357100

Centisome position: 56.35

GC content: 49.82

Gene sequence:

>273_bases
ATGACCAAGGCTGATCTGGTGGAAAAAATTGCTGAAAAAGCGAACCTGACTAAGGCCAACGCAGAACGTTCTCTGAACGC
TTTTCTTGAATCCGTTGAGGATGTGCTGGTAAAGGAAGGCAAACTTACCCTTACCGGCTTCGGTACTTTTATGGTTGAAA
CCCGTCAGGAACGTCAGGGCCGCAACCCCCGCACGGGCGATCCCATTGTCATTCCTGCTGCCAAGGTTGTTAAGTTCCGT
CCCGGCAAACTGCTTAAAGACGCAGTAAAGTAA

Upstream 100 bases:

>100_bases
AAAAAAATCTCAAAGTGCCGGTTGCAGATATTGTGTGGGTGCGCCGGTCGTAACGCTTTAGCCTGATAATCCTGCAAACC
CTGAGAGGAGGAGACGGCAG

Downstream 100 bases:

>100_bases
CCATCGGAGGCTTTTGTCATGATGCTGCATGGTGAAACCATACAGAGCCCGCTCCCCATGGATATCCCGTGGTGGGCTCC
TGATCACTTTGTGTTTTTCG

Product: histone-like DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MTKADLVEKIAEKANLTKANAERSLNAFLESVEDVLVKEGKLTLTGFGTFMVETRQERQGRNPRTGDPIVIPAAKVVKFR
PGKLLKDAVK

Sequences:

>Translated_90_residues
MTKADLVEKIAEKANLTKANAERSLNAFLESVEDVLVKEGKLTLTGFGTFMVETRQERQGRNPRTGDPIVIPAAKVVKFR
PGKLLKDAVK
>Mature_89_residues
TKADLVEKIAEKANLTKANAERSLNAFLESVEDVLVKEGKLTLTGFGTFMVETRQERQGRNPRTGDPIVIPAAKVVKFRP
GKLLKDAVK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=51.685393258427, Blast_Score=89, Evalue=7e-20,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=48.8888888888889, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=38.8888888888889, Blast_Score=69, Evalue=7e-14,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=34.0909090909091, Blast_Score=65, Evalue=8e-13,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9925; Mature: 9794

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKADLVEKIAEKANLTKANAERSLNAFLESVEDVLVKEGKLTLTGFGTFMVETRQERQG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHEECHHHHCC
RNPRTGDPIVIPAAKVVKFRPGKLLKDAVK
CCCCCCCCEEECCHHHEEECCCHHHHHHCC
>Mature Secondary Structure 
TKADLVEKIAEKANLTKANAERSLNAFLESVEDVLVKEGKLTLTGFGTFMVETRQERQG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHEECHHHHCC
RNPRTGDPIVIPAAKVVKFRPGKLLKDAVK
CCCCCCCCEEECCHHHEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]