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The map label for this gene is dnaG [H]

Identifier: 78357096

GI number: 78357096

Start: 2096628

End: 2098358

Strand: Reverse

Name: dnaG [H]

Synonym: Dde_2053

Alternate gene names: 78357096

Gene position: 2098358-2096628 (Counterclockwise)

Preceding gene: 78357097

Following gene: 78357095

Centisome position: 56.25

GC content: 54.65

Gene sequence:

>1731_bases
ATGTCGGGTCACGATAATTCGGCTGTACAGGCCATCAAGTCCAGACTGAATCTTGCCGAGCTGGCGCGGCGATATGTTGA
ATTGCGCCGTATAGGCAACCGCTGGATGGCACCGTGTCCTTTTCATCAGGAGACCAAACCTTCTTTTTCCATCAATGAGG
AAGAGGGCTTTTTCTACTGCTTCGGTTGTCAGGCTTCCGGTGATATAATTGATTTTTACAGCCGGATAAACGGACTTGAA
TTCCGGGAGGCGTTGGAGCAGCTTGCCGAAGAGGCGGGGGTGACTCTGGGCCAGCGGCAGGCCGATCCCAGAGAAGAGCA
GGAGCGCAAGTTCCGGTCCCGCTGTTATGAAATGTACGAGCTGGCGACAAAGCATTTCCGCAGAAATCTGCAGTCCGCGG
CCGGTGCTGAGTGCCGCAGCTACATTGCCGACCGGGGAATACCCGACGAGATTGTCAGTTCTTTTGAGCTGGGCTGGAGC
AAAAGAGAGTGGGCTGCACTTTCCGATGAATTACAGCGTGCCGGGTATGCTCCCCGCGATGCCACCCGTGCCGGGCTGCT
TTCATCCAACGACTCCGGCAGAATGTATGACAGGTTCCGGGGCAGACTTATTTTTCCCATCAAAAATCTTTCGGGAAAAG
TTATTGCTTTTGGCGGCAGAATAATTAGCTCAGAAGATCAGGCGAAATATATAAACAGCAGTGACAGTACCATATACAAA
AAGGGCGACCATCTGTACGGCCTGTATCAGGCCAGAAGGTCCATATCGCAGCATAAGCGTGCTCTTTTGACAGAAGGGTA
TATGGACGTGCTGGCTTTGCACCAGTTCGGGCATACCAATGCCTGCGGCGTGCTGGGTACGGCCCTGACGGAGGCGCAGG
TAAAGCGTCTCGGCGGATTTTGTTCGCATGTTGACCTGCTTTTTGACGGTGACGGTCCCGGGCGCAAGGCCGCTTTGCGC
AGCTGCGAAATGATGCTGGTGAAAGGTATGGATTGTCGTGTGGTGCTGATGCCCGAGGGCGAAGATATCGACAGCCTGCT
GCACCAGCAGGGGGCACAGGCTTTTCGTGAATTGACGGAATCAGCTCCCGACGGGCTTGAATACTGCATCAGGACGCTAC
GGGATTCTTATTCGCCGCGGGAGATTCTGGACTGGACGGCGGCTTTTCTGCGCCAGCTCGACCGGCCCGATCTGGCAAGC
TTTTATGTGACGCGTCTTGCCGGCGGTCTGGGGCTGCAGGAAGCGGACCTGCGGGCTTCGCTTTCTTCGCCTGCAGGGCG
AAACAGACAGCAGCGTGCAGCGGGCAATCGTGTGCAGACGGGAAAGGAAAAGAAACTTGAGATGCATCTTATGGAGTTTC
TCGTCCGCTTTCCGCACCATATTTCCAACCTGCAGGCCCGCGGCGCGGAGGAAGTGCTTGTTTCGCGCTGGGCCAGAGAT
ATGTGGGATAAACTTTTGGCGTTCGGAGCCGATGAAGCGCCGAACCATTTTTCTGAAGAACAGAAAGCGCTTTGGATCAG
ATGCAGGGTTGCCAGCCCTATTCCGGCTGAGAACGAGCGGCAGGAACTTGAGGACTTATACTCCAAGTTGGCCGAACTGA
ATCAGAAAGAACAAAGCAGGATGATGCTGGCGGCACTGAGACAGCTGAGCAGTGATGCAAATTCCGAAGCGGGTTTTGAG
CTTCTTCGGGCCATGAAAGATAATCTGGGGAGATCCGATGGGCAACATTAA

Upstream 100 bases:

>100_bases
TGCGGTCGATGCTTTCCGCCTTGCACCCGAAGGGCGCGGCGGCGACGGCATGACCATTGTCGAATTGAAGTAGCCTTGCA
TTTGCGGAGATTGTGCCATT

Downstream 100 bases:

>100_bases
GGAAATCCAGCAAATCAAGACCCTGATTGCCAAAGGCAAGGTCGCGGGCTTTCTCACGTTTGAGGAAGTTAACAAAGCTT
TGCCGGCAGAGGTCAACACG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MSGHDNSAVQAIKSRLNLAELARRYVELRRIGNRWMAPCPFHQETKPSFSINEEEGFFYCFGCQASGDIIDFYSRINGLE
FREALEQLAEEAGVTLGQRQADPREEQERKFRSRCYEMYELATKHFRRNLQSAAGAECRSYIADRGIPDEIVSSFELGWS
KREWAALSDELQRAGYAPRDATRAGLLSSNDSGRMYDRFRGRLIFPIKNLSGKVIAFGGRIISSEDQAKYINSSDSTIYK
KGDHLYGLYQARRSISQHKRALLTEGYMDVLALHQFGHTNACGVLGTALTEAQVKRLGGFCSHVDLLFDGDGPGRKAALR
SCEMMLVKGMDCRVVLMPEGEDIDSLLHQQGAQAFRELTESAPDGLEYCIRTLRDSYSPREILDWTAAFLRQLDRPDLAS
FYVTRLAGGLGLQEADLRASLSSPAGRNRQQRAAGNRVQTGKEKKLEMHLMEFLVRFPHHISNLQARGAEEVLVSRWARD
MWDKLLAFGADEAPNHFSEEQKALWIRCRVASPIPAENERQELEDLYSKLAELNQKEQSRMMLAALRQLSSDANSEAGFE
LLRAMKDNLGRSDGQH

Sequences:

>Translated_576_residues
MSGHDNSAVQAIKSRLNLAELARRYVELRRIGNRWMAPCPFHQETKPSFSINEEEGFFYCFGCQASGDIIDFYSRINGLE
FREALEQLAEEAGVTLGQRQADPREEQERKFRSRCYEMYELATKHFRRNLQSAAGAECRSYIADRGIPDEIVSSFELGWS
KREWAALSDELQRAGYAPRDATRAGLLSSNDSGRMYDRFRGRLIFPIKNLSGKVIAFGGRIISSEDQAKYINSSDSTIYK
KGDHLYGLYQARRSISQHKRALLTEGYMDVLALHQFGHTNACGVLGTALTEAQVKRLGGFCSHVDLLFDGDGPGRKAALR
SCEMMLVKGMDCRVVLMPEGEDIDSLLHQQGAQAFRELTESAPDGLEYCIRTLRDSYSPREILDWTAAFLRQLDRPDLAS
FYVTRLAGGLGLQEADLRASLSSPAGRNRQQRAAGNRVQTGKEKKLEMHLMEFLVRFPHHISNLQARGAEEVLVSRWARD
MWDKLLAFGADEAPNHFSEEQKALWIRCRVASPIPAENERQELEDLYSKLAELNQKEQSRMMLAALRQLSSDANSEAGFE
LLRAMKDNLGRSDGQH
>Mature_575_residues
SGHDNSAVQAIKSRLNLAELARRYVELRRIGNRWMAPCPFHQETKPSFSINEEEGFFYCFGCQASGDIIDFYSRINGLEF
REALEQLAEEAGVTLGQRQADPREEQERKFRSRCYEMYELATKHFRRNLQSAAGAECRSYIADRGIPDEIVSSFELGWSK
REWAALSDELQRAGYAPRDATRAGLLSSNDSGRMYDRFRGRLIFPIKNLSGKVIAFGGRIISSEDQAKYINSSDSTIYKK
GDHLYGLYQARRSISQHKRALLTEGYMDVLALHQFGHTNACGVLGTALTEAQVKRLGGFCSHVDLLFDGDGPGRKAALRS
CEMMLVKGMDCRVVLMPEGEDIDSLLHQQGAQAFRELTESAPDGLEYCIRTLRDSYSPREILDWTAAFLRQLDRPDLASF
YVTRLAGGLGLQEADLRASLSSPAGRNRQQRAAGNRVQTGKEKKLEMHLMEFLVRFPHHISNLQARGAEEVLVSRWARDM
WDKLLAFGADEAPNHFSEEQKALWIRCRVASPIPAENERQELEDLYSKLAELNQKEQSRMMLAALRQLSSDANSEAGFEL
LRAMKDNLGRSDGQH

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=360, Percent_Identity=36.1111111111111, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65116; Mature: 64985

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGHDNSAVQAIKSRLNLAELARRYVELRRIGNRWMAPCPFHQETKPSFSINEEEGFFYC
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEE
FGCQASGDIIDFYSRINGLEFREALEQLAEEAGVTLGQRQADPREEQERKFRSRCYEMYE
EECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LATKHFRRNLQSAAGAECRSYIADRGIPDEIVSSFELGWSKREWAALSDELQRAGYAPRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ATRAGLLSSNDSGRMYDRFRGRLIFPIKNLSGKVIAFGGRIISSEDQAKYINSSDSTIYK
HHHHCCCCCCCCCCHHHHHCCEEEEEECCCCCCEEEECCEEECCCCHHHHCCCCCCEEEE
KGDHLYGLYQARRSISQHKRALLTEGYMDVLALHQFGHTNACGVLGTALTEAQVKRLGGF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCH
CSHVDLLFDGDGPGRKAALRSCEMMLVKGMDCRVVLMPEGEDIDSLLHQQGAQAFRELTE
HHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SAPDGLEYCIRTLRDSYSPREILDWTAAFLRQLDRPDLASFYVTRLAGGLGLQEADLRAS
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHH
LSSPAGRNRQQRAAGNRVQTGKEKKLEMHLMEFLVRFPHHISNLQARGAEEVLVSRWARD
HCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MWDKLLAFGADEAPNHFSEEQKALWIRCRVASPIPAENERQELEDLYSKLAELNQKEQSR
HHHHHHHCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MMLAALRQLSSDANSEAGFELLRAMKDNLGRSDGQH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGHDNSAVQAIKSRLNLAELARRYVELRRIGNRWMAPCPFHQETKPSFSINEEEGFFYC
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEE
FGCQASGDIIDFYSRINGLEFREALEQLAEEAGVTLGQRQADPREEQERKFRSRCYEMYE
EECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LATKHFRRNLQSAAGAECRSYIADRGIPDEIVSSFELGWSKREWAALSDELQRAGYAPRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
ATRAGLLSSNDSGRMYDRFRGRLIFPIKNLSGKVIAFGGRIISSEDQAKYINSSDSTIYK
HHHHCCCCCCCCCCHHHHHCCEEEEEECCCCCCEEEECCEEECCCCHHHHCCCCCCEEEE
KGDHLYGLYQARRSISQHKRALLTEGYMDVLALHQFGHTNACGVLGTALTEAQVKRLGGF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCH
CSHVDLLFDGDGPGRKAALRSCEMMLVKGMDCRVVLMPEGEDIDSLLHQQGAQAFRELTE
HHHEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SAPDGLEYCIRTLRDSYSPREILDWTAAFLRQLDRPDLASFYVTRLAGGLGLQEADLRAS
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHH
LSSPAGRNRQQRAAGNRVQTGKEKKLEMHLMEFLVRFPHHISNLQARGAEEVLVSRWARD
HCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MWDKLLAFGADEAPNHFSEEQKALWIRCRVASPIPAENERQELEDLYSKLAELNQKEQSR
HHHHHHHCCCCCCCCHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
MMLAALRQLSSDANSEAGFELLRAMKDNLGRSDGQH
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]