Click here to switch to the map view.

The map label for this gene is ycdT [H]

Identifier: 78357093

GI number: 78357093

Start: 2092991

End: 2094103

Strand: Reverse

Name: ycdT [H]

Synonym: Dde_2050

Alternate gene names: 78357093

Gene position: 2094103-2092991 (Counterclockwise)

Preceding gene: 78357094

Following gene: 78357085

Centisome position: 56.14

GC content: 53.19

Gene sequence:

>1113_bases
ATGAAGATTAGCCCGCTTAAAGATGTTGCCGGTTCGGTGCATGCTGTCTGCGACGGCCGCCTGCACTGCGATTTTTCTGC
TGACGGCCGTATGTGCGAAGCTCTTGACAACGCAGGAGTGCCCCGTGATGCAAAGTGGCGGACTCTTGTTTTGTACATGC
GAGGCATGCAGGAATATTCCTATCTGACAGATGAACAGAAAATGCAGGTTCAGCAACTGCTTGTTGACCTGTTGCAAAAC
AAAGATTTTTCAGACCGGAATTACGAGAGAATTCTGGAGCGTGACAGACAGATTCTTGTGGCACCCTATGAGCAGCGTCT
TAACAGGGCCATGCAGGAAACCATGGCTCTTGCCGGCGAGTTCCGCAGTATCCTGCTGCGCAGAAAAGGTGATGTGGAGC
ATCTGGAATCCCGCAGCATTCAGGCGGTGACCGAGGGCAGAGAGCCCGAAGAAGTCGTGGCTTTGCTGAAAGATACCTTT
CGTGAAGTTCTGCTTGAGATGGAGCGTGACGCCGCAGCCATGACAGAACTGACCCAGCGTGATCCTTTGAGTGGTCTGCA
TAACAGGCGCGCATTTGATGCCTTTCTTTCCGGCGCGGTTCAAAGCTGGCGCAGAGAGGGAACCCCGCTTTCTCTCATCA
TGTTGGACATTGATAATTTTAAAAATTTCAACGACGAATACGGCCACCGCATAGGGGATCAGGCCATTCAGACCGTGGCC
AGAATTTTACAGGAGGCTGTCCGCGGGCATGCTGCCGAACACGAAAAAGATTTTCTTGTTGCCCGTTACGGGGGCGAGGA
GTTTTCCGTGGTGCTCAGAGGGGCACTTGCTTCTCTGGCGGAAAAAATTGCAGAGAGCGCCCGCAGCAGCATGGAGAATT
TTAACTTTGTCATCCGCAATAACAGCGGGGAGGTGGTGCGAAGCGGCATCAGCATCACCCTGAGTGCCGGAGTGGCTTTT
GCCAGCAGTTTGTGGCGCGGCGCTTATGAGGATAATCTTATCGATTCCGCAGATCGGGCTCTTTATGAGGCCAAACGCGG
CGGACGTAATCTGGTGTTCAGGTACCGTGATGATCAGGACGGCAACAGCCGGTATGAGCTTGTGGACAGCTGA

Upstream 100 bases:

>100_bases
TGAAAGCTTCATGGCAGGCATGGCTCCGGCAAGGTCATGCGATGTCTGGCCGTTACAGTGATCATGTAGTGATAATCCTT
CTGCTGACAGGTATAGTGTT

Downstream 100 bases:

>100_bases
ACGCAGACAGCGAATTGAATAAAAAAGGCCGGGTGACCGGCCTTGATTTTTATCGGGCCCCGCCGTGGCGGGAATTGTGT
TCTGCTAGCCCAGTTTGGCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKISPLKDVAGSVHAVCDGRLHCDFSADGRMCEALDNAGVPRDAKWRTLVLYMRGMQEYSYLTDEQKMQVQQLLVDLLQN
KDFSDRNYERILERDRQILVAPYEQRLNRAMQETMALAGEFRSILLRRKGDVEHLESRSIQAVTEGREPEEVVALLKDTF
REVLLEMERDAAAMTELTQRDPLSGLHNRRAFDAFLSGAVQSWRREGTPLSLIMLDIDNFKNFNDEYGHRIGDQAIQTVA
RILQEAVRGHAAEHEKDFLVARYGGEEFSVVLRGALASLAEKIAESARSSMENFNFVIRNNSGEVVRSGISITLSAGVAF
ASSLWRGAYEDNLIDSADRALYEAKRGGRNLVFRYRDDQDGNSRYELVDS

Sequences:

>Translated_370_residues
MKISPLKDVAGSVHAVCDGRLHCDFSADGRMCEALDNAGVPRDAKWRTLVLYMRGMQEYSYLTDEQKMQVQQLLVDLLQN
KDFSDRNYERILERDRQILVAPYEQRLNRAMQETMALAGEFRSILLRRKGDVEHLESRSIQAVTEGREPEEVVALLKDTF
REVLLEMERDAAAMTELTQRDPLSGLHNRRAFDAFLSGAVQSWRREGTPLSLIMLDIDNFKNFNDEYGHRIGDQAIQTVA
RILQEAVRGHAAEHEKDFLVARYGGEEFSVVLRGALASLAEKIAESARSSMENFNFVIRNNSGEVVRSGISITLSAGVAF
ASSLWRGAYEDNLIDSADRALYEAKRGGRNLVFRYRDDQDGNSRYELVDS
>Mature_370_residues
MKISPLKDVAGSVHAVCDGRLHCDFSADGRMCEALDNAGVPRDAKWRTLVLYMRGMQEYSYLTDEQKMQVQQLLVDLLQN
KDFSDRNYERILERDRQILVAPYEQRLNRAMQETMALAGEFRSILLRRKGDVEHLESRSIQAVTEGREPEEVVALLKDTF
REVLLEMERDAAAMTELTQRDPLSGLHNRRAFDAFLSGAVQSWRREGTPLSLIMLDIDNFKNFNDEYGHRIGDQAIQTVA
RILQEAVRGHAAEHEKDFLVARYGGEEFSVVLRGALASLAEKIAESARSSMENFNFVIRNNSGEVVRSGISITLSAGVAF
ASSLWRGAYEDNLIDSADRALYEAKRGGRNLVFRYRDDQDGNSRYELVDS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=176, Percent_Identity=35.7954545454545, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=33.1460674157303, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693134, Length=202, Percent_Identity=35.1485148514851, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787816, Length=201, Percent_Identity=35.8208955223881, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87082007, Length=174, Percent_Identity=32.183908045977, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081881, Length=253, Percent_Identity=28.8537549407115, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787541, Length=178, Percent_Identity=30.8988764044944, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787802, Length=143, Percent_Identity=32.8671328671329, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=29.0502793296089, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788956, Length=193, Percent_Identity=28.4974093264249, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788381, Length=180, Percent_Identity=30.5555555555556, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41981; Mature: 41981

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISPLKDVAGSVHAVCDGRLHCDFSADGRMCEALDNAGVPRDAKWRTLVLYMRGMQEYS
CCCCCHHHHHCCHHHHCCCEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YLTDEQKMQVQQLLVDLLQNKDFSDRNYERILERDRQILVAPYEQRLNRAMQETMALAGE
HCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHH
FRSILLRRKGDVEHLESRSIQAVTEGREPEEVVALLKDTFREVLLEMERDAAAMTELTQR
HHHHHHHCCCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPLSGLHNRRAFDAFLSGAVQSWRREGTPLSLIMLDIDNFKNFNDEYGHRIGDQAIQTVA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
RILQEAVRGHAAEHEKDFLVARYGGEEFSVVLRGALASLAEKIAESARSSMENFNFVIRN
HHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
NSGEVVRSGISITLSAGVAFASSLWRGAYEDNLIDSADRALYEAKRGGRNLVFRYRDDQD
CCCHHHHCCCEEEEHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
GNSRYELVDS
CCCCCCCCCC
>Mature Secondary Structure
MKISPLKDVAGSVHAVCDGRLHCDFSADGRMCEALDNAGVPRDAKWRTLVLYMRGMQEYS
CCCCCHHHHHCCHHHHCCCEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YLTDEQKMQVQQLLVDLLQNKDFSDRNYERILERDRQILVAPYEQRLNRAMQETMALAGE
HCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHH
FRSILLRRKGDVEHLESRSIQAVTEGREPEEVVALLKDTFREVLLEMERDAAAMTELTQR
HHHHHHHCCCCHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPLSGLHNRRAFDAFLSGAVQSWRREGTPLSLIMLDIDNFKNFNDEYGHRIGDQAIQTVA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
RILQEAVRGHAAEHEKDFLVARYGGEEFSVVLRGALASLAEKIAESARSSMENFNFVIRN
HHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEC
NSGEVVRSGISITLSAGVAFASSLWRGAYEDNLIDSADRALYEAKRGGRNLVFRYRDDQD
CCCHHHHCCCEEEEHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
GNSRYELVDS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]