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The map label for this gene is hslU

Identifier: 78357057

GI number: 78357057

Start: 2056343

End: 2057692

Strand: Direct

Name: hslU

Synonym: Dde_2014

Alternate gene names: 78357057

Gene position: 2056343-2057692 (Clockwise)

Preceding gene: 78357056

Following gene: 78357058

Centisome position: 55.13

GC content: 53.11

Gene sequence:

>1350_bases
ATGAGCGTACTCACGCCCAGAGAAATCGTTTCAGAACTGGACAAGTATATAATAGGTCAGGAACAGGCAAAACGAATGGT
GGCGGTGGCACTGCGCAACCGCTGGCGCCGGCAACAGCTGGAACCTGCCATCAGAGATGAAGTGGCTCCCAAAAACATCA
TCATGATGGGCCCGACCGGTGTGGGCAAGACGGAAATCGCCAGACGGCTCGCCAGGCTTTCTTCCTCCCCCTTTCTGAAA
GTCGAAGCCACCAAGTTCACCGAAGTGGGTTATGTGGGGCGTGATGTGGAGTCCATGATCCGCGACCTGATGGAGATCAG
TGTCAATCTGGTACGGGAAGAGGAACGGGACAGTGTCAAAACCCGCGCGGAAGCCGCAGCAGAAGAACGGCTTCTTGACC
TGCTGCTCCCCTCTTCCCCTGCTGTAAACACAAGCTTTGACACCCCGCAGACACCTGCAGGCGGCGCAGAAAATACCCGC
AACAAGCTGAGAACGCTCTGGCGTGAAGGCAGGCTTGATCACAGGGAAGTGGAAATGGAAGTGGAAATGCAGTCCGGTCC
GCAAATGGATGTCATGACCATGCCGGGCATGGAGGACATCGGTTCGCAGTTCAAGGATATGTTCAGCAAGGCATTTCCGC
CTAAACGCAAACGGCGCCGCATGAAGGTGGGTGACGCCTATGCCATTCTGCTGCAGGAAGAATCGGAACGGCTCATCGAC
CATGAAAAGGTCATAGAACTGGCAAGAGAACGCGCAGAACAGACAGGCATTATCTTTCTGGATGAAATAGACAAAATAGC
CAGCAGTCAGCAAGGCGGCAAAACCGCCGACATATCGAGAGAAGGTGTCCAGCGTGACCTGCTGCCCATTGTCGAAGGCT
CTGCGGTCAATACCAAATACGGCATCATACACACTGACCATATCCTCTTCATCGGAGCGGGGGCATTCCACTACAGCAAA
CCTTCCGACCTTATTCCCGAACTGCAGGGGCGTTTTCCGTTGCGGGTGGAGCTGACGGCGCTTGGCAGCAAGGAATTTCT
CCGCATTCTTACAGAGCCGAAAAACGCCCTGACCGTACAGTACAAAGCTCTGCTGGCCACCGAAGGTGTGCAGATAGAAT
ATACCGAAGATGCGCTGGAGGCTGTGGCCGACTTTGCACAGGAAGCAAATCAGGAAACTGAAAACATCGGCGCACGTCGC
CTGTATACCATAATGGAAAAAATTCTTGCAGATCTTTCTTTTGAAGCTCCTGACAAAAGCGGAGAGCGTATAGTCATAGA
CAGGGAATATGTCCGGCAGCATCTTGCAGATATCACTGCAAACAGGGATCTTACCCGTTACATACTCTAA

Upstream 100 bases:

>100_bases
CCACGCATGCTGCTGCTGTCTCTGCCCGCCGTCCGCTTTTTTCACGGCCCGGACAGCCACACACTGCGCGCTGATATCAT
ATGTCTTTCAAGGAGCAACC

Downstream 100 bases:

>100_bases
ACCGTGATACGGAGCATACCGTGCAGAACGATGCGTTCAAATCCAAGGTTCTCATTGAATCCCTGCCTTACTTCCGCCAG
TTCAGCGGTGAAACAGTTGT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSVLTPREIVSELDKYIIGQEQAKRMVAVALRNRWRRQQLEPAIRDEVAPKNIIMMGPTGVGKTEIARRLARLSSSPFLK
VEATKFTEVGYVGRDVESMIRDLMEISVNLVREEERDSVKTRAEAAAEERLLDLLLPSSPAVNTSFDTPQTPAGGAENTR
NKLRTLWREGRLDHREVEMEVEMQSGPQMDVMTMPGMEDIGSQFKDMFSKAFPPKRKRRRMKVGDAYAILLQEESERLID
HEKVIELARERAEQTGIIFLDEIDKIASSQQGGKTADISREGVQRDLLPIVEGSAVNTKYGIIHTDHILFIGAGAFHYSK
PSDLIPELQGRFPLRVELTALGSKEFLRILTEPKNALTVQYKALLATEGVQIEYTEDALEAVADFAQEANQETENIGARR
LYTIMEKILADLSFEAPDKSGERIVIDREYVRQHLADITANRDLTRYIL

Sequences:

>Translated_449_residues
MSVLTPREIVSELDKYIIGQEQAKRMVAVALRNRWRRQQLEPAIRDEVAPKNIIMMGPTGVGKTEIARRLARLSSSPFLK
VEATKFTEVGYVGRDVESMIRDLMEISVNLVREEERDSVKTRAEAAAEERLLDLLLPSSPAVNTSFDTPQTPAGGAENTR
NKLRTLWREGRLDHREVEMEVEMQSGPQMDVMTMPGMEDIGSQFKDMFSKAFPPKRKRRRMKVGDAYAILLQEESERLID
HEKVIELARERAEQTGIIFLDEIDKIASSQQGGKTADISREGVQRDLLPIVEGSAVNTKYGIIHTDHILFIGAGAFHYSK
PSDLIPELQGRFPLRVELTALGSKEFLRILTEPKNALTVQYKALLATEGVQIEYTEDALEAVADFAQEANQETENIGARR
LYTIMEKILADLSFEAPDKSGERIVIDREYVRQHLADITANRDLTRYIL
>Mature_448_residues
SVLTPREIVSELDKYIIGQEQAKRMVAVALRNRWRRQQLEPAIRDEVAPKNIIMMGPTGVGKTEIARRLARLSSSPFLKV
EATKFTEVGYVGRDVESMIRDLMEISVNLVREEERDSVKTRAEAAAEERLLDLLLPSSPAVNTSFDTPQTPAGGAENTRN
KLRTLWREGRLDHREVEMEVEMQSGPQMDVMTMPGMEDIGSQFKDMFSKAFPPKRKRRRMKVGDAYAILLQEESERLIDH
EKVIELARERAEQTGIIFLDEIDKIASSQQGGKTADISREGVQRDLLPIVEGSAVNTKYGIIHTDHILFIGAGAFHYSKP
SDLIPELQGRFPLRVELTALGSKEFLRILTEPKNALTVQYKALLATEGVQIEYTEDALEAVADFAQEANQETENIGARRL
YTIMEKILADLSFEAPDKSGERIVIDREYVRQHLADITANRDLTRYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=253, Percent_Identity=31.2252964426877, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1790366, Length=452, Percent_Identity=53.7610619469027, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1786642, Length=102, Percent_Identity=48.0392156862745, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71982905, Length=263, Percent_Identity=29.6577946768061, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982908, Length=263, Percent_Identity=29.6577946768061, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988663, Length=258, Percent_Identity=26.7441860465116, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319704, Length=122, Percent_Identity=36.8852459016393, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24648289, Length=248, Percent_Identity=31.4516129032258, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648291, Length=248, Percent_Identity=31.4516129032258, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_DESDG (Q30ZT5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388506.1
- ProteinModelPortal:   Q30ZT5
- SMR:   Q30ZT5
- STRING:   Q30ZT5
- GeneID:   3757022
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2014
- NMPDR:   fig|207559.3.peg.746
- eggNOG:   COG1220
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   DDES207559:DDE_2014-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 50783; Mature: 50652

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 261-261 BINDING 327-327 BINDING 399-399

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLTPREIVSELDKYIIGQEQAKRMVAVALRNRWRRQQLEPAIRDEVAPKNIIMMGPTG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLARLSSSPFLKVEATKFTEVGYVGRDVESMIRDLMEISVNLVREEERDSVK
CCHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRAEAAAEERLLDLLLPSSPAVNTSFDTPQTPAGGAENTRNKLRTLWREGRLDHREVEME
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEE
VEMQSGPQMDVMTMPGMEDIGSQFKDMFSKAFPPKRKRRRMKVGDAYAILLQEESERLID
EEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECHHHHHHH
HEKVIELARERAEQTGIIFLDEIDKIASSQQGGKTADISREGVQRDLLPIVEGSAVNTKY
HHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
GIIHTDHILFIGAGAFHYSKPSDLIPELQGRFPLRVELTALGSKEFLRILTEPKNALTVQ
CEEEECCEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEE
YKALLATEGVQIEYTEDALEAVADFAQEANQETENIGARRLYTIMEKILADLSFEAPDKS
HHHHHHCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GERIVIDREYVRQHLADITANRDLTRYIL
CCEEEEEHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SVLTPREIVSELDKYIIGQEQAKRMVAVALRNRWRRQQLEPAIRDEVAPKNIIMMGPTG
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEECCCC
VGKTEIARRLARLSSSPFLKVEATKFTEVGYVGRDVESMIRDLMEISVNLVREEERDSVK
CCHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
TRAEAAAEERLLDLLLPSSPAVNTSFDTPQTPAGGAENTRNKLRTLWREGRLDHREVEME
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEE
VEMQSGPQMDVMTMPGMEDIGSQFKDMFSKAFPPKRKRRRMKVGDAYAILLQEESERLID
EEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEEECHHHHHHH
HEKVIELARERAEQTGIIFLDEIDKIASSQQGGKTADISREGVQRDLLPIVEGSAVNTKY
HHHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
GIIHTDHILFIGAGAFHYSKPSDLIPELQGRFPLRVELTALGSKEFLRILTEPKNALTVQ
CEEEECCEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEE
YKALLATEGVQIEYTEDALEAVADFAQEANQETENIGARRLYTIMEKILADLSFEAPDKS
HHHHHHCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GERIVIDREYVRQHLADITANRDLTRYIL
CCEEEEEHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA