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The map label for this gene is gacS [H]

Identifier: 78356988

GI number: 78356988

Start: 1994972

End: 1997002

Strand: Reverse

Name: gacS [H]

Synonym: Dde_1945

Alternate gene names: 78356988

Gene position: 1997002-1994972 (Counterclockwise)

Preceding gene: 78356989

Following gene: 78356987

Centisome position: 53.54

GC content: 50.42

Gene sequence:

>2031_bases
ATGTTTATCAAAGCCCCTTTCACGGGGCTTTTTTCTTTTTGTCCGTTCTCAGCTGCCCTCCGCTTCCGCGCCGTCTTGTC
TTTACGTTATTTACCCTCAGTTGTTTTATGCCGGAATATCATTTCCGCCCTGCTGTCAGCTATCTATATTGATGGCATGA
TTGCTCCTGATTTCAAGCCGTTTTTTTTAAAGAAAAGCAGAAATTCCTCAGGCGTGTTCACAGCCTTTCTCACGCTGTTC
ATTCCTGCGGTGTTTCTGTCTGCTCTGGCCATCGCGCTTTTTCTCTATGCGGATATGCAGCACCGGATCGATCTGGAAAA
AAGGCAGGAATCCGCCAGCGTGAGCAGCGCATATGACGGTATTTCCATGGAACTGAATATCGTTTTTCAGAGTATACTGA
ATGCGCTTAACAGAGGGTCTTTGAAGGAATACGCCGCGGTTCCCTCCTCTGAAAATTTTGCACGTCTGGTCTCTGACATG
TACTCCTTTGCACTGGATAACAATCAGTTTGATCAGATTCGTATGCTTGATCCCGCAGGCATGGAACTGCTGCGCATCAA
CCGGAGTGACAGCGGGCCGGTTCTGGTTCCCGAAGAGAATCTGGCTGATAAATCAAGGCGCTATTATTATAAAGATATTA
TGGAGCTGGCTCCTGACAATATCTACATATCACGTCTGGACCTTAATATTGAAAACGGTGTGGTTGAAGTCCCCTACAAG
CCTATCATCCGTATCGGGACAAAGCTGCTGGACAGAAGCGGCAAACTTCTGGGTATCATGGTTTTTAATTACCTTGCGGA
TAATATGCTGAACGGTGCCTGGATGCTGCGCAGCTCCGATCAGGGTGTGCTGATGCTGCTGGATCATGAGGGATACTGGT
TGCAGTCGGATACCCCTTCGCAGCACTGGGGGTTCATGTTCGGTGATACGCTGGGTATTAATTTTGCCCAGCGGCATCCG
GGGGTCTGGCAGCAGATGCTGCAGACGGGCTCCGGGCAGATCGCCACCTCTGACGGGGTGTTTACGTATGCAGCCGTCGC
GGCCTCTCCGGCGGGCCACACCATCTATTCCTACAAGCAGATACATGTGCCGGATAAGTATCTGCGCGACTGGTTCATTG
TTTCCTACATGCCTTTTCCGTCCATGACGGAGTTTCTGTGGGCAGAAGGACTGCCCTACTCCATGGCATTTTTTCTTGTA
TGTCTGCTGAGCATGGTCGGGACATTCATGCGGGCCGGTTTTCTGCAGGAGCGCAAAGAGGCGGTGCTTGCTTTGCGGAA
AAGCGAAGAACGTTTCAGAGGGATTGTCGAAACTTCGCACGACTGGATATGGGAGATGGATCTGAGCGGGTGGATTACCT
ATTCAAGCCCGCGCATACGGGAAATACTGGGATACAGTGTGGAAGAGGCATACGGAAAGCGTATTTTTGACCTTATGTCC
TACAATGATGCCCGCACCGCATGGGGGCATTTTATGAAAGCAGTGGAAAACGCCGCCGCCGTTGAACACCACGAAAACGC
TTTTCAGCATAAGGCCGGCCTGCGGGTGGTGCTGGAATCGAACATGGTGCCCATGGAAGGTGACTCCGGAGAACTGCTGG
GCTTCAGGGGCATTGCTCGGGACGTTACGGAAAAGAAGATTGTTCTTTCCCAGCTGGAAACGGCGCGTAAAGAGGCTGAA
AATGCCAGCCGGGCCAAAGGAGAGTTTCTGGCCCGTATGAGTCATGAAATCCGCACCCCCATGAATGCCGTCATCGGCAT
GTGCCATCTGGCGCTGAAAACAGCACTTACGCCTAAGCAATTTGATTATCTTACAAAAATACAGTCTTCCGCCAATTCGT
TGCTCGGGGTCATTAATGATGTCCTTGATTTTTCAAAAATCGAGGCCGGCAAACTGGATATGGAGCGCGTGCGGTTTAAC
CTGGAAACCACTTTGACAAATGTGGCCAGTGTCAATTCCATTGCGGCATCAGTGTTACCGGACAATGATATTGTCACCCC
TGTCTGGGACAATAGAAATGTCATCCCATGA

Upstream 100 bases:

>100_bases
CCGCAGGAACAGTCCTGCACACATGGCGAGGTAGCTCAGTTGGTTAGAGCATGCGGCTCATATCCGCAGTGTCGGGGGTT
CAATTCCCTCCCTCGCTACC

Downstream 100 bases:

>100_bases
AGACACGTGGTGACGTACTCATGAAGGCAGAGGAAGCGCGGCGAGCGTTTGTGATCAAGCAGGCAGTGGCCGGAGCGTTC
ACGGTTCGGGAGGCGGGAGA

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MFIKAPFTGLFSFCPFSAALRFRAVLSLRYLPSVVLCRNIISALLSAIYIDGMIAPDFKPFFLKKSRNSSGVFTAFLTLF
IPAVFLSALAIALFLYADMQHRIDLEKRQESASVSSAYDGISMELNIVFQSILNALNRGSLKEYAAVPSSENFARLVSDM
YSFALDNNQFDQIRMLDPAGMELLRINRSDSGPVLVPEENLADKSRRYYYKDIMELAPDNIYISRLDLNIENGVVEVPYK
PIIRIGTKLLDRSGKLLGIMVFNYLADNMLNGAWMLRSSDQGVLMLLDHEGYWLQSDTPSQHWGFMFGDTLGINFAQRHP
GVWQQMLQTGSGQIATSDGVFTYAAVAASPAGHTIYSYKQIHVPDKYLRDWFIVSYMPFPSMTEFLWAEGLPYSMAFFLV
CLLSMVGTFMRAGFLQERKEAVLALRKSEERFRGIVETSHDWIWEMDLSGWITYSSPRIREILGYSVEEAYGKRIFDLMS
YNDARTAWGHFMKAVENAAAVEHHENAFQHKAGLRVVLESNMVPMEGDSGELLGFRGIARDVTEKKIVLSQLETARKEAE
NASRAKGEFLARMSHEIRTPMNAVIGMCHLALKTALTPKQFDYLTKIQSSANSLLGVINDVLDFSKIEAGKLDMERVRFN
LETTLTNVASVNSIAASVLPDNDIVTPVWDNRNVIP

Sequences:

>Translated_676_residues
MFIKAPFTGLFSFCPFSAALRFRAVLSLRYLPSVVLCRNIISALLSAIYIDGMIAPDFKPFFLKKSRNSSGVFTAFLTLF
IPAVFLSALAIALFLYADMQHRIDLEKRQESASVSSAYDGISMELNIVFQSILNALNRGSLKEYAAVPSSENFARLVSDM
YSFALDNNQFDQIRMLDPAGMELLRINRSDSGPVLVPEENLADKSRRYYYKDIMELAPDNIYISRLDLNIENGVVEVPYK
PIIRIGTKLLDRSGKLLGIMVFNYLADNMLNGAWMLRSSDQGVLMLLDHEGYWLQSDTPSQHWGFMFGDTLGINFAQRHP
GVWQQMLQTGSGQIATSDGVFTYAAVAASPAGHTIYSYKQIHVPDKYLRDWFIVSYMPFPSMTEFLWAEGLPYSMAFFLV
CLLSMVGTFMRAGFLQERKEAVLALRKSEERFRGIVETSHDWIWEMDLSGWITYSSPRIREILGYSVEEAYGKRIFDLMS
YNDARTAWGHFMKAVENAAAVEHHENAFQHKAGLRVVLESNMVPMEGDSGELLGFRGIARDVTEKKIVLSQLETARKEAE
NASRAKGEFLARMSHEIRTPMNAVIGMCHLALKTALTPKQFDYLTKIQSSANSLLGVINDVLDFSKIEAGKLDMERVRFN
LETTLTNVASVNSIAASVLPDNDIVTPVWDNRNVIP
>Mature_676_residues
MFIKAPFTGLFSFCPFSAALRFRAVLSLRYLPSVVLCRNIISALLSAIYIDGMIAPDFKPFFLKKSRNSSGVFTAFLTLF
IPAVFLSALAIALFLYADMQHRIDLEKRQESASVSSAYDGISMELNIVFQSILNALNRGSLKEYAAVPSSENFARLVSDM
YSFALDNNQFDQIRMLDPAGMELLRINRSDSGPVLVPEENLADKSRRYYYKDIMELAPDNIYISRLDLNIENGVVEVPYK
PIIRIGTKLLDRSGKLLGIMVFNYLADNMLNGAWMLRSSDQGVLMLLDHEGYWLQSDTPSQHWGFMFGDTLGINFAQRHP
GVWQQMLQTGSGQIATSDGVFTYAAVAASPAGHTIYSYKQIHVPDKYLRDWFIVSYMPFPSMTEFLWAEGLPYSMAFFLV
CLLSMVGTFMRAGFLQERKEAVLALRKSEERFRGIVETSHDWIWEMDLSGWITYSSPRIREILGYSVEEAYGKRIFDLMS
YNDARTAWGHFMKAVENAAAVEHHENAFQHKAGLRVVLESNMVPMEGDSGELLGFRGIARDVTEKKIVLSQLETARKEAE
NASRAKGEFLARMSHEIRTPMNAVIGMCHLALKTALTPKQFDYLTKIQSSANSLLGVINDVLDFSKIEAGKLDMERVRFN
LETTLTNVASVNSIAASVLPDNDIVTPVWDNRNVIP

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=101, Percent_Identity=57.4257425742574, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI48994928, Length=172, Percent_Identity=29.6511627906977, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081816, Length=100, Percent_Identity=44, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1788713, Length=120, Percent_Identity=35, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI145693157, Length=121, Percent_Identity=38.8429752066116, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76158; Mature: 76158

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIKAPFTGLFSFCPFSAALRFRAVLSLRYLPSVVLCRNIISALLSAIYIDGMIAPDFKP
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
FFLKKSRNSSGVFTAFLTLFIPAVFLSALAIALFLYADMQHRIDLEKRQESASVSSAYDG
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
ISMELNIVFQSILNALNRGSLKEYAAVPSSENFARLVSDMYSFALDNNQFDQIRMLDPAG
CCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCC
MELLRINRSDSGPVLVPEENLADKSRRYYYKDIMELAPDNIYISRLDLNIENGVVEVPYK
CEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEECCCH
PIIRIGTKLLDRSGKLLGIMVFNYLADNMLNGAWMLRSSDQGVLMLLDHEGYWLQSDTPS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCEEECCCCCC
QHWGFMFGDTLGINFAQRHPGVWQQMLQTGSGQIATSDGVFTYAAVAASPAGHTIYSYKQ
CCCCEEECCCCCCCHHHCCCHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCCCEEEEEEE
IHVPDKYLRDWFIVSYMPFPSMTEFLWAEGLPYSMAFFLVCLLSMVGTFMRAGFLQERKE
ECCCHHHHHHEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLALRKSEERFRGIVETSHDWIWEMDLSGWITYSSPRIREILGYSVEEAYGKRIFDLMS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHC
YNDARTAWGHFMKAVENAAAVEHHENAFQHKAGLRVVLESNMVPMEGDSGELLGFRGIAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEHHHHHH
DVTEKKIVLSQLETARKEAENASRAKGEFLARMSHEIRTPMNAVIGMCHLALKTALTPKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
FDYLTKIQSSANSLLGVINDVLDFSKIEAGKLDMERVRFNLETTLTNVASVNSIAASVLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DNDIVTPVWDNRNVIP
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFIKAPFTGLFSFCPFSAALRFRAVLSLRYLPSVVLCRNIISALLSAIYIDGMIAPDFKP
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCC
FFLKKSRNSSGVFTAFLTLFIPAVFLSALAIALFLYADMQHRIDLEKRQESASVSSAYDG
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
ISMELNIVFQSILNALNRGSLKEYAAVPSSENFARLVSDMYSFALDNNQFDQIRMLDPAG
CCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCC
MELLRINRSDSGPVLVPEENLADKSRRYYYKDIMELAPDNIYISRLDLNIENGVVEVPYK
CEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCEEECCCH
PIIRIGTKLLDRSGKLLGIMVFNYLADNMLNGAWMLRSSDQGVLMLLDHEGYWLQSDTPS
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCEEECCCCCC
QHWGFMFGDTLGINFAQRHPGVWQQMLQTGSGQIATSDGVFTYAAVAASPAGHTIYSYKQ
CCCCEEECCCCCCCHHHCCCHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCCCEEEEEEE
IHVPDKYLRDWFIVSYMPFPSMTEFLWAEGLPYSMAFFLVCLLSMVGTFMRAGFLQERKE
ECCCHHHHHHEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLALRKSEERFRGIVETSHDWIWEMDLSGWITYSSPRIREILGYSVEEAYGKRIFDLMS
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHC
YNDARTAWGHFMKAVENAAAVEHHENAFQHKAGLRVVLESNMVPMEGDSGELLGFRGIAR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEHHHHHH
DVTEKKIVLSQLETARKEAENASRAKGEFLARMSHEIRTPMNAVIGMCHLALKTALTPKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
FDYLTKIQSSANSLLGVINDVLDFSKIEAGKLDMERVRFNLETTLTNVASVNSIAASVLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
DNDIVTPVWDNRNVIP
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]