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The map label for this gene is anfA [H]

Identifier: 78356678

GI number: 78356678

Start: 1686704

End: 1688434

Strand: Direct

Name: anfA [H]

Synonym: Dde_1635

Alternate gene names: 78356678

Gene position: 1686704-1688434 (Clockwise)

Preceding gene: 78356677

Following gene: 78356684

Centisome position: 45.22

GC content: 53.44

Gene sequence:

>1731_bases
GTGTGTCGGTTTCATCCGGCACGGCTGTGTGCGACTATGTTTTTTGCGGTGACTTGCCGTGAACAGCACAATGTGTTTGA
CCTGAACCATGCTGGTAGTCGACCTAGGTCTCAGCGCCGTGGCCGGACGCCGCAATCAGCACTTATTATGAACCAGATCA
ATACCCAAGATTCCAGATTACAGCCATATCTAGGCACGCTGCAGCGCATTGTTTCCGACATGGGCCCGCAGCGTCCGTTT
CAGTCTTCGCTGAAGTCATTACTGCGCACGCTGGCTGAAAATCACCATTTCAAACGCCCCCACCTTGTTATATTCGATCC
CGAAACACAAACGCTCAAGCTTAGCCTGACGCATGATCCTACCAAAGCGCAGGATGTGGAATATTCGCCGGGAGTGGGGG
TGACCGGACAGGTTTTCAGTTCCGGACAGCCTGTTATCGTGCCGAGGATGAAAGATCATCCTTCGTTTCTCAATAAAGCG
TTCGGCCGTTCGGAAGAAGAGCTGGCAACTCTGGGCTTTATATGCGTGCCTGTGCTCGGACCGGGCGAAGAAGCCTCTCC
CCGGGAGGTTATCGGCACACTGAGCGTGGACACGCCCATAACTGACCTGCCCTCTCTGGAAGGACAGTGCCGTTTTCTGC
AGGTTGTTGCCGGTATGATAGCCAACCAGGCTGCCTACCTGCAGGAAGAAATGGCCCGTCAGAAGCATATGATGACGCAG
GGCCTCATAGGCGGTGACGTTGCCGAAAACACGGTGCAGGCCGCTAATATTGTGGCTGCGTCCAAGTCCATGCGCCTTGT
GCTCAATCAGGTGGCTCAGGTTGGTCCCAGCAGGGCGACGGCTTTGCTGCGGGGCGAATCGGGCACCGGCAAAGAGCTTC
TTGCAGAGGCCATACATCAGGCCAGCCCCCGGCGTGATATGCCGCTTATCAAGCTTAACTGCGCGGCTTTACCATCCGAT
CTGGTGGAAAGCGAGCTGTTCGGCTATCAGAAGGGCGCGTTTACAGGTGCTGTCCAGAATAAAAAAGGCCTTTTTGAGCT
TGCACACAAGGGGACGCTGTTTCTTGATGAAATAGGTGAATTGAGTGCCTCGGCTCAGGCTAAAGTGCTGCGTGCCATTC
AGGAACAGGAAATTCAGCGGCTCGGCAGTGAACAGACCATTTCCGTTGATGTCCGCCTGATCTGCGCCACACACCAGCAT
CTGGAAGAGCTTGTGGAGAGCGGCAGTTTCCGTGAGGATCTTTATTACCGGATCAATGTGTTTCCGGTGTTCATTCCTCC
GCTGCGCGAACGCCGCGAAGATATTCTTCCCGTTGCTGAGCATTTTCTTAAAGAATACGCAGAGGAATATCAGAAAAGCA
TCAAGCGTATTTCCACCCCTGCCATCGACCTGCTTACACAGTATCACTGGCCCGGTAACATCCGCGAACTGAAAAACTGC
ATAGAGCGTGCAGTGCTGGTGTGTGATGAACAGGTTATCCGTACGTATCACATGCCCCCTTCGCTGCAGACAGCAGAAAG
CACAGCAACAGATTCCAGTCTGTCGTTCTGCGAGGCGGTGGCCAAGTTTGAGCAGGAACTACTGGTTGACGCGCTGAAAA
AAGCCCGTGGCAATATGTTGCAGGCTGCCCGCGATTTGCGCGTAAGCTACCGTATTGTTAACTATAAAGTTAAGAAGTAC
GGTATCGATGCCAAAAAGTTCGCTGTCAGTAAAGGACGCATGGGCAGATAG

Upstream 100 bases:

>100_bases
CCAGAGCCTGAAACAGCATATGCAGAGCGGCAGCTAGCCCTGCCTGTCATGCGGCGAGCGGACACCGGCGGCGGAGCATG
GTTGTTGCCCTGTTTCTGCC

Downstream 100 bases:

>100_bases
ACGCCTCTGGCGGATGAAAGTAAAAGCGGAGGTTCCGGCGGGAACCTCCGCTTTTTTGTATTTCGCCGGGCGGCACGCAG
CATACTGCGGGCCGGAGTAA

Product: Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MCRFHPARLCATMFFAVTCREQHNVFDLNHAGSRPRSQRRGRTPQSALIMNQINTQDSRLQPYLGTLQRIVSDMGPQRPF
QSSLKSLLRTLAENHHFKRPHLVIFDPETQTLKLSLTHDPTKAQDVEYSPGVGVTGQVFSSGQPVIVPRMKDHPSFLNKA
FGRSEEELATLGFICVPVLGPGEEASPREVIGTLSVDTPITDLPSLEGQCRFLQVVAGMIANQAAYLQEEMARQKHMMTQ
GLIGGDVAENTVQAANIVAASKSMRLVLNQVAQVGPSRATALLRGESGTGKELLAEAIHQASPRRDMPLIKLNCAALPSD
LVESELFGYQKGAFTGAVQNKKGLFELAHKGTLFLDEIGELSASAQAKVLRAIQEQEIQRLGSEQTISVDVRLICATHQH
LEELVESGSFREDLYYRINVFPVFIPPLRERREDILPVAEHFLKEYAEEYQKSIKRISTPAIDLLTQYHWPGNIRELKNC
IERAVLVCDEQVIRTYHMPPSLQTAESTATDSSLSFCEAVAKFEQELLVDALKKARGNMLQAARDLRVSYRIVNYKVKKY
GIDAKKFAVSKGRMGR

Sequences:

>Translated_576_residues
MCRFHPARLCATMFFAVTCREQHNVFDLNHAGSRPRSQRRGRTPQSALIMNQINTQDSRLQPYLGTLQRIVSDMGPQRPF
QSSLKSLLRTLAENHHFKRPHLVIFDPETQTLKLSLTHDPTKAQDVEYSPGVGVTGQVFSSGQPVIVPRMKDHPSFLNKA
FGRSEEELATLGFICVPVLGPGEEASPREVIGTLSVDTPITDLPSLEGQCRFLQVVAGMIANQAAYLQEEMARQKHMMTQ
GLIGGDVAENTVQAANIVAASKSMRLVLNQVAQVGPSRATALLRGESGTGKELLAEAIHQASPRRDMPLIKLNCAALPSD
LVESELFGYQKGAFTGAVQNKKGLFELAHKGTLFLDEIGELSASAQAKVLRAIQEQEIQRLGSEQTISVDVRLICATHQH
LEELVESGSFREDLYYRINVFPVFIPPLRERREDILPVAEHFLKEYAEEYQKSIKRISTPAIDLLTQYHWPGNIRELKNC
IERAVLVCDEQVIRTYHMPPSLQTAESTATDSSLSFCEAVAKFEQELLVDALKKARGNMLQAARDLRVSYRIVNYKVKKY
GIDAKKFAVSKGRMGR
>Mature_576_residues
MCRFHPARLCATMFFAVTCREQHNVFDLNHAGSRPRSQRRGRTPQSALIMNQINTQDSRLQPYLGTLQRIVSDMGPQRPF
QSSLKSLLRTLAENHHFKRPHLVIFDPETQTLKLSLTHDPTKAQDVEYSPGVGVTGQVFSSGQPVIVPRMKDHPSFLNKA
FGRSEEELATLGFICVPVLGPGEEASPREVIGTLSVDTPITDLPSLEGQCRFLQVVAGMIANQAAYLQEEMARQKHMMTQ
GLIGGDVAENTVQAANIVAASKSMRLVLNQVAQVGPSRATALLRGESGTGKELLAEAIHQASPRRDMPLIKLNCAALPSD
LVESELFGYQKGAFTGAVQNKKGLFELAHKGTLFLDEIGELSASAQAKVLRAIQEQEIQRLGSEQTISVDVRLICATHQH
LEELVESGSFREDLYYRINVFPVFIPPLRERREDILPVAEHFLKEYAEEYQKSIKRISTPAIDLLTQYHWPGNIRELKNC
IERAVLVCDEQVIRTYHMPPSLQTAESTATDSSLSFCEAVAKFEQELLVDALKKARGNMLQAARDLRVSYRIVNYKVKKY
GIDAKKFAVSKGRMGR

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=317, Percent_Identity=46.0567823343849, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1790437, Length=303, Percent_Identity=48.1848184818482, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI87082117, Length=290, Percent_Identity=48.2758620689655, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1788905, Length=312, Percent_Identity=44.5512820512821, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1789087, Length=356, Percent_Identity=40.4494382022472, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=40.6940063091483, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI87082152, Length=341, Percent_Identity=39.5894428152493, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790299, Length=331, Percent_Identity=40.1812688821752, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1787583, Length=356, Percent_Identity=38.2022471910112, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=42.8571428571429, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=38.871473354232, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=40.7079646017699, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI87081858, Length=297, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 64231; Mature: 64231

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRFHPARLCATMFFAVTCREQHNVFDLNHAGSRPRSQRRGRTPQSALIMNQINTQDSRL
CCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCHHHHHHHHCCCCHHHC
QPYLGTLQRIVSDMGPQRPFQSSLKSLLRTLAENHHFKRPHLVIFDPETQTLKLSLTHDP
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCC
TKAQDVEYSPGVGVTGQVFSSGQPVIVPRMKDHPSFLNKAFGRSEEELATLGFICVPVLG
CCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHEEECCC
PGEEASPREVIGTLSVDTPITDLPSLEGQCRFLQVVAGMIANQAAYLQEEMARQKHMMTQ
CCCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIGGDVAENTVQAANIVAASKSMRLVLNQVAQVGPSRATALLRGESGTGKELLAEAIHQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCHHHHHHHHHH
ASPRRDMPLIKLNCAALPSDLVESELFGYQKGAFTGAVQNKKGLFELAHKGTLFLDEIGE
CCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHH
LSASAQAKVLRAIQEQEIQRLGSEQTISVDVRLICATHQHLEELVESGSFREDLYYRINV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCEEEEEEE
FPVFIPPLRERREDILPVAEHFLKEYAEEYQKSIKRISTPAIDLLTQYHWPGNIRELKNC
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHH
IERAVLVCDEQVIRTYHMPPSLQTAESTATDSSLSFCEAVAKFEQELLVDALKKARGNML
HHHHHHHHCHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
QAARDLRVSYRIVNYKVKKYGIDAKKFAVSKGRMGR
HHHHHHHHEEEEEEEEEHHHCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MCRFHPARLCATMFFAVTCREQHNVFDLNHAGSRPRSQRRGRTPQSALIMNQINTQDSRL
CCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCHHHHHHHHCCCCHHHC
QPYLGTLQRIVSDMGPQRPFQSSLKSLLRTLAENHHFKRPHLVIFDPETQTLKLSLTHDP
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCC
TKAQDVEYSPGVGVTGQVFSSGQPVIVPRMKDHPSFLNKAFGRSEEELATLGFICVPVLG
CCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHEEECCC
PGEEASPREVIGTLSVDTPITDLPSLEGQCRFLQVVAGMIANQAAYLQEEMARQKHMMTQ
CCCCCCHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLIGGDVAENTVQAANIVAASKSMRLVLNQVAQVGPSRATALLRGESGTGKELLAEAIHQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCHHHHHHHHHH
ASPRRDMPLIKLNCAALPSDLVESELFGYQKGAFTGAVQNKKGLFELAHKGTLFLDEIGE
CCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHH
LSASAQAKVLRAIQEQEIQRLGSEQTISVDVRLICATHQHLEELVESGSFREDLYYRINV
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHCCCCCCCEEEEEEE
FPVFIPPLRERREDILPVAEHFLKEYAEEYQKSIKRISTPAIDLLTQYHWPGNIRELKNC
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHH
IERAVLVCDEQVIRTYHMPPSLQTAESTATDSSLSFCEAVAKFEQELLVDALKKARGNML
HHHHHHHHCHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
QAARDLRVSYRIVNYKVKKYGIDAKKFAVSKGRMGR
HHHHHHHHEEEEEEEEEHHHCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]