Click here to switch to the map view.

The map label for this gene is cobD [H]

Identifier: 78356572

GI number: 78356572

Start: 1547614

End: 1548567

Strand: Direct

Name: cobD [H]

Synonym: Dde_1529

Alternate gene names: 78356572

Gene position: 1547614-1548567 (Clockwise)

Preceding gene: 78356571

Following gene: 78356574

Centisome position: 41.49

GC content: 63.1

Gene sequence:

>954_bases
ATGGACTGGTGGTTGATGTTGGTTCCTTTTGTGGCGGTACTGCTGGATATGCTGGCGGGCGACCCCGCCTGTCTGCCGCA
TCCGGTACGGGCGGTGGGCAGACTGGCCGACATGGTGGAACGGCGCGTGCGCGGGTACGGCAGTTCGGTGCGTATGGGCG
CGCTGGCGGCCGGACTGGTGGTGCTGCCTGTGGCTGTGGTTGTCTGGGGGCTGTGCGCACTGCCTGTGGCCGGAGCATTG
GCCACGTTGTATTTTGCCTATGCCGGACTTGCACTGGGCAGCCTGCTGAAAGAAGGTGAGGCCGCTCTTGCTGCAGTGCA
GCACGGCACACTGGAAGAGGCCCGGCATGCCGTGGGCATGCTGGTAAGCCGCGATACTTCTGCCATGAACCGTGATGAAC
TGTACCGTTCTCTTGCCGAGTCGGTAAGCGAGAACAGCAATGATGGTTTTGTGGCACCTTTCTTTTATCTGGTTCTGGGG
GGCCCTGTGGTGCTGTGGGTGTACAAGGCTGTCAGCACGCTGGATTCCATGTGGGGCTACAGGACAGGACGCTGGGCCAG
TTTCGGCTGTGCAGCGGCACGCCTTGATGATGTTCTGGCGTACGTTCCGGCGCGTATCACCGCGTTTTTCATGCTGGCGG
CAGCCCGGCTGATGCTGGTTCCCACCGCGTTTGACCTGCGCGGAACATGGCGCAGGGCTGCATCTGATGCCGGACTTATG
GACAGCCCCAATGCCGGCTGGCCCATGGCCATGGCCGCGTGGCTTCTTTCCCGCCGTATGGGCGGACCTGCTGTCTATGA
CGGGGTGACGGTGAACAAGCCGCTGCTCGGGCCTGTCACCGGCGTGTGGCGGGCTGAGGACATCCGCATGCTGCTGCGGC
TTGTCCGCCTTTCCTGTGTGCTGGGGGCGTGGGGTCTCTGGTTGCTTGCGCTGTGGCTGCATGCCGCGTTGTAG

Upstream 100 bases:

>100_bases
AACGGAAGCCTGCGGCGCGGCATGGGACATGCCGCTTTGATGTGGTTATGCACACATGCTGTGCCTGATGTGGTGCGGGA
AAAGAAGAGAGGCTGGCGTT

Downstream 100 bases:

>100_bases
TTCTGGATTGCCGCCGAGGTGTCCGGCAGAAAACATCCCGCCGGCAGGCCGCGTTCATTCGCGTCAGGATTTCTGCTCTT
TCAGCGCTGTTCCTGCTGCA

Product: adenosylcobinamide-phosphate synthase

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MDWWLMLVPFVAVLLDMLAGDPACLPHPVRAVGRLADMVERRVRGYGSSVRMGALAAGLVVLPVAVVVWGLCALPVAGAL
ATLYFAYAGLALGSLLKEGEAALAAVQHGTLEEARHAVGMLVSRDTSAMNRDELYRSLAESVSENSNDGFVAPFFYLVLG
GPVVLWVYKAVSTLDSMWGYRTGRWASFGCAAARLDDVLAYVPARITAFFMLAAARLMLVPTAFDLRGTWRRAASDAGLM
DSPNAGWPMAMAAWLLSRRMGGPAVYDGVTVNKPLLGPVTGVWRAEDIRMLLRLVRLSCVLGAWGLWLLALWLHAAL

Sequences:

>Translated_317_residues
MDWWLMLVPFVAVLLDMLAGDPACLPHPVRAVGRLADMVERRVRGYGSSVRMGALAAGLVVLPVAVVVWGLCALPVAGAL
ATLYFAYAGLALGSLLKEGEAALAAVQHGTLEEARHAVGMLVSRDTSAMNRDELYRSLAESVSENSNDGFVAPFFYLVLG
GPVVLWVYKAVSTLDSMWGYRTGRWASFGCAAARLDDVLAYVPARITAFFMLAAARLMLVPTAFDLRGTWRRAASDAGLM
DSPNAGWPMAMAAWLLSRRMGGPAVYDGVTVNKPLLGPVTGVWRAEDIRMLLRLVRLSCVLGAWGLWLLALWLHAAL
>Mature_317_residues
MDWWLMLVPFVAVLLDMLAGDPACLPHPVRAVGRLADMVERRVRGYGSSVRMGALAAGLVVLPVAVVVWGLCALPVAGAL
ATLYFAYAGLALGSLLKEGEAALAAVQHGTLEEARHAVGMLVSRDTSAMNRDELYRSLAESVSENSNDGFVAPFFYLVLG
GPVVLWVYKAVSTLDSMWGYRTGRWASFGCAAARLDDVLAYVPARITAFFMLAAARLMLVPTAFDLRGTWRRAASDAGLM
DSPNAGWPMAMAAWLLSRRMGGPAVYDGVTVNKPLLGPVTGVWRAEDIRMLLRLVRLSCVLGAWGLWLLALWLHAAL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34192; Mature: 34192

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWWLMLVPFVAVLLDMLAGDPACLPHPVRAVGRLADMVERRVRGYGSSVRMGALAAGLV
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VLPVAVVVWGLCALPVAGALATLYFAYAGLALGSLLKEGEAALAAVQHGTLEEARHAVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LVSRDTSAMNRDELYRSLAESVSENSNDGFVAPFFYLVLGGPVVLWVYKAVSTLDSMWGY
HHHCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
RTGRWASFGCAAARLDDVLAYVPARITAFFMLAAARLMLVPTAFDLRGTWRRAASDAGLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
DSPNAGWPMAMAAWLLSRRMGGPAVYDGVTVNKPLLGPVTGVWRAEDIRMLLRLVRLSCV
CCCCCCCHHHHHHHHHHHHCCCCHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAWGLWLLALWLHAAL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDWWLMLVPFVAVLLDMLAGDPACLPHPVRAVGRLADMVERRVRGYGSSVRMGALAAGLV
CCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VLPVAVVVWGLCALPVAGALATLYFAYAGLALGSLLKEGEAALAAVQHGTLEEARHAVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LVSRDTSAMNRDELYRSLAESVSENSNDGFVAPFFYLVLGGPVVLWVYKAVSTLDSMWGY
HHHCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
RTGRWASFGCAAARLDDVLAYVPARITAFFMLAAARLMLVPTAFDLRGTWRRAASDAGLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
DSPNAGWPMAMAAWLLSRRMGGPAVYDGVTVNKPLLGPVTGVWRAEDIRMLLRLVRLSCV
CCCCCCCHHHHHHHHHHHHCCCCHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGAWGLWLLALWLHAAL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]