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The map label for this gene is trmL [H]

Identifier: 78356571

GI number: 78356571

Start: 1547057

End: 1547515

Strand: Direct

Name: trmL [H]

Synonym: Dde_1528

Alternate gene names: 78356571

Gene position: 1547057-1547515 (Clockwise)

Preceding gene: 78356568

Following gene: 78356572

Centisome position: 41.47

GC content: 57.3

Gene sequence:

>459_bases
ATGCATATAGTTCTTTTTCAGCCGGAAATTCCGCCCAATACGGGAAACGTCGCGCGGCTGTGCGCGGCCACGCGGACCAG
TCTGCATCTTATCGAACCGCTGGGGTTCAGTGTGGATGACAAACATGTACGTCGGGCCGGTCTGGATTACTGGCCGCATG
TGGACCTGACCGTGTGGCCGGGGTGGTATGCCTTTATGCGCGAGGTGGGCAGCAGAGTCCGTCTTGTGATGACCAGTGCA
CGCGGCGGCACAGCTGTGCAGGATTTCCGGTTCAACAAAAATGATGCGCTGGTGTTCGGGCCGGAAAGCAGAGGGCTGCC
TGCCCATATGCTGGACGGAAGCGGACATGTTGTGCGTATTCCCATATGGGGTGAAGTACGCAGCCTTAATATGTCGACAG
CGGCGGGTATCGTATTGTATCAGGCACTTGCGGCTTCCGGTGAACTGGCTGGCAGATAA

Upstream 100 bases:

>100_bases
GTAGCCGCCGGTGCGTACCCTTGCAGCACCTGCGGTACCGGCTTGATTCTCCTGCCGGTTCAGGTATGACCTGAACCGGC
TTTTTTTATGAGGAGCGTAT

Downstream 100 bases:

>100_bases
CGGAAGCCTGCGGCGCGGCATGGGACATGCCGCTTTGATGTGGTTATGCACACATGCTGTGCCTGATGTGGTGCGGGAAA
AGAAGAGAGGCTGGCGTTAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MHIVLFQPEIPPNTGNVARLCAATRTSLHLIEPLGFSVDDKHVRRAGLDYWPHVDLTVWPGWYAFMREVGSRVRLVMTSA
RGGTAVQDFRFNKNDALVFGPESRGLPAHMLDGSGHVVRIPIWGEVRSLNMSTAAGIVLYQALAASGELAGR

Sequences:

>Translated_152_residues
MHIVLFQPEIPPNTGNVARLCAATRTSLHLIEPLGFSVDDKHVRRAGLDYWPHVDLTVWPGWYAFMREVGSRVRLVMTSA
RGGTAVQDFRFNKNDALVFGPESRGLPAHMLDGSGHVVRIPIWGEVRSLNMSTAAGIVLYQALAASGELAGR
>Mature_152_residues
MHIVLFQPEIPPNTGNVARLCAATRTSLHLIEPLGFSVDDKHVRRAGLDYWPHVDLTVWPGWYAFMREVGSRVRLVMTSA
RGGTAVQDFRFNKNDALVFGPESRGLPAHMLDGSGHVVRIPIWGEVRSLNMSTAAGIVLYQALAASGELAGR

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=144, Percent_Identity=45.8333333333333, Blast_Score=128, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 16690; Mature: 16690

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIVLFQPEIPPNTGNVARLCAATRTSLHLIEPLGFSVDDKHVRRAGLDYWPHVDLTVWP
CEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCEEECC
GWYAFMREVGSRVRLVMTSARGGTAVQDFRFNKNDALVFGPESRGLPAHMLDGSGHVVRI
CHHHHHHHHCCEEEEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCEEEECCCCCEEEE
PIWGEVRSLNMSTAAGIVLYQALAASGELAGR
EECCCEEECCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MHIVLFQPEIPPNTGNVARLCAATRTSLHLIEPLGFSVDDKHVRRAGLDYWPHVDLTVWP
CEEEEECCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCEEECC
GWYAFMREVGSRVRLVMTSARGGTAVQDFRFNKNDALVFGPESRGLPAHMLDGSGHVVRI
CHHHHHHHHCCEEEEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCEEEECCCCCEEEE
PIWGEVRSLNMSTAAGIVLYQALAASGELAGR
EECCCEEECCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360 [H]